Cytochrome c from Equus caballus (horse)

Enzyme Description

Species

Equus caballus (horse) ↗

Extremophile

EC Number

1.11.1.5 ↗

Sequence

Length: 105 amino acids

MGDVEKGKKIFVQKCAQCHTVEKGGKHKTGPNLHGLFGRKTGQAPGFTYTDANKNKGITWKEETLMEYLENPKKYIPGTKMIFAGIKKKTEREDLIAYLKKATNE

Rafael Vazquez-Duhalt et al. (1993) — Effect of water-miscible organic solvents on the catalytic activity of cytochrome c

Enzyme and Microbial Technology · doi:10.1016/0141-0229(93)90169-3 ↗ · Activity - Classical Activity - Michaelis-Menten

72 measurements ▼

Database ID

UniProt: P00004 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Commercially purchased

Experimental Data (72 measurements)

72 measurements — page 3 of 4

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Methanol	50%	1.768	3.259	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% methanol \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Methanol	60%	1.768	2.76874	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% methanol \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Methanol	70%	1.768	2.563	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% methanol \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Methanol	80%	1.768	0.87756	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% methanol \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Methanol	85%	1.768	0.33339	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% methanol \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Methanol	90%	1.768	0.2829	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% methanol \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Methanol	100%	1.768	0.051	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% methanol \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Methanol	75%	1.768	1.421839	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% methanol \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten	Km (mM)	Tetrahydrofuran (THF)	50%	21.5	1.2	mM	6 mM phosphate buffer	6.1	Room temperature	H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in mM) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten	Km (mM)	Tetrahydrofuran (THF)	40%	21.5	1.7	mM	6 mM phosphate buffer	6.1	Room temperature	H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in mM) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten	Km (mM)	Tetrahydrofuran (THF)	30%	21.5	6.2	mM	6 mM phosphate buffer	6.1	Room temperature	H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in mM) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten	Km (mM)	Tetrahydrofuran (THF)	20%	21.5	23.7	mM	6 mM phosphate buffer	6.1	Room temperature	H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in mM) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten	kcat (deltaA603/(min*nmol))	Tetrahydrofuran (THF)	90%	15.5	3.7	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten	kcat (deltaA603/(min*nmol))	Tetrahydrofuran (THF)	80%	15.5	2.6	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten	kcat (deltaA603/(min*nmol))	Tetrahydrofuran (THF)	70%	15.5	0.3	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten	kcat (deltaA603/(min*nmol))	Tetrahydrofuran (THF)	60%	15.5	1.9	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten	kcat (deltaA603/(min*nmol))	Tetrahydrofuran (THF)	50%	15.5	2.1	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten	kcat (deltaA603/(min*nmol))	Tetrahydrofuran (THF)	40%	15.5	2.7	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten	kcat (deltaA603/(min*nmol))	Tetrahydrofuran (THF)	20%	15.5	21.0	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten	kcat (deltaA603/(min*nmol))	Tetrahydrofuran (THF)	30%	15.5	6.5	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride	H2O , Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Acetonitrile	20%	1.72972	2.36036	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% acetonitrile \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Dimethylformamide (DMF)	30%	1.38541	1.40625	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% DMF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Dimethylformamide (DMF)	20%	1.38541	1.385416	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% DMF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Acetonitrile	80%	1.72972	0.036	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% acetonitrile \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Acetonitrile	70%	1.72972	0.25225	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% acetonitrile \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Acetonitrile	60%	1.72972	1.15315	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% acetonitrile \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Acetonitrile	50%	1.72972	1.333333	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% acetonitrile \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Acetonitrile	40%	1.72972	1.765765	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% acetonitrile \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Acetonitrile	30%	1.72972	2.0	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% acetonitrile \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Dimethylformamide (DMF)	35%	1.38541	1.3541	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% DMF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Isopropanol	100%	0.9615	0.0769	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% isopropanol \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Isopropanol	80%	0.9615	0.2499999	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% isopropanol \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Isopropanol	75%	0.9615	0.326923	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% isopropanol \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Isopropanol	70%	0.9615	0.96153	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% isopropanol \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Isopropanol	65%	0.9615	2.61538461	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% isopropanol \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Isopropanol	60%	0.9615	2.34615384	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% isopropanol \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Isopropanol	50%	0.9615	2.365384615	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% isopropanol \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Isopropanol	40%	0.9615	2.4615384	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% isopropanol \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	30%	0.87857	0.84285	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	100%	0.87857	0.3428571428	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	90%	0.87857	0.59285714	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	80%	0.87857	0.2857142	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	70%	0.87857	0.1999	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	65%	0.87857	0.721428	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	60%	0.87857	0.75	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	50%	0.87857	0.73571	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	40%	0.87857	0.72142	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Isopropanol	30%	0.9615	2.019	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% isopropanol \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	20%	0.87857	0.72142	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Dimethylformamide (DMF)	100%	1.38541	0.0	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% DMF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Dimethylformamide (DMF)	80%	1.38541	0.0208333	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% DMF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Dimethylformamide (DMF)	60%	1.38541	0.114	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% DMF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Dimethylformamide (DMF)	55%	1.38541	0.18749999	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% DMF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Dimethylformamide (DMF)	50%	1.38541	0.854166	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% DMF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Dimethylformamide (DMF)	45%	1.38541	0.9166	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% DMF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Dimethylformamide (DMF)	40%	1.38541	1.28125	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% DMF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Isopropanol	20%	0.9615	1.846	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% isopropanol \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Methanol	20%	1.768	1.66745	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% methanol \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Methanol	30%	1.768	2.3973328	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% methanol \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Methanol	40%	1.768	2.607	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% methanol \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Methanol	50%	1.768	3.259	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% methanol \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Methanol	60%	1.768	2.76874	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% methanol \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Methanol	70%	1.768	2.563	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% methanol \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Methanol	80%	1.768	0.87756	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% methanol \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Methanol	85%	1.768	0.33339	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% methanol \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Methanol	90%	1.768	0.2829	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% methanol \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Methanol	100%	1.768	0.051	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% methanol \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Classical	Initial reaction rate measured by absorbance spectrophotometry (pinacyanol absorbance measurement, 603 nm) in the presence of organic solvent	Methanol	75%	1.768	1.421839	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	? mM H₂O₂ (Hydrogen Peroxide), 5 µgramme/ml Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% methanol \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten	Km (mM)	Tetrahydrofuran (THF)	50%	21.5	1.2	mM	6 mM phosphate buffer	6.1	Room temperature	H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in mM) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten	Km (mM)	Tetrahydrofuran (THF)	40%	21.5	1.7	mM	6 mM phosphate buffer	6.1	Room temperature	H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in mM) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten	Km (mM)	Tetrahydrofuran (THF)	30%	21.5	6.2	mM	6 mM phosphate buffer	6.1	Room temperature	H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in mM) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten	Km (mM)	Tetrahydrofuran (THF)	20%	21.5	23.7	mM	6 mM phosphate buffer	6.1	Room temperature	H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in mM) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten	kcat (deltaA603/(min*nmol))	Tetrahydrofuran (THF)	90%	15.5	3.7	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten	kcat (deltaA603/(min*nmol))	Tetrahydrofuran (THF)	80%	15.5	2.6	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten	kcat (deltaA603/(min*nmol))	Tetrahydrofuran (THF)	70%	15.5	0.3	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten	kcat (deltaA603/(min*nmol))	Tetrahydrofuran (THF)	60%	15.5	1.9	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten	kcat (deltaA603/(min*nmol))	Tetrahydrofuran (THF)	50%	15.5	2.1	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten	kcat (deltaA603/(min*nmol))	Tetrahydrofuran (THF)	40%	15.5	2.7	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten	kcat (deltaA603/(min*nmol))	Tetrahydrofuran (THF)	20%	15.5	21.0	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten	kcat (deltaA603/(min*nmol))	Tetrahydrofuran (THF)	30%	15.5	6.5	deltaA603/(min*nmol)	6 mM phosphate buffer	6.1	Room temperature	H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(min*nmol)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten	Km (mM)	Tetrahydrofuran (THF)	60%	21.5	1.3	mM	6 mM phosphate buffer	6.1	Room temperature	H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in mM) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten	Km (mM)	Tetrahydrofuran (THF)	70%	21.5	0.7	mM	6 mM phosphate buffer	6.1	Room temperature	H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in mM) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten	Km (mM)	Tetrahydrofuran (THF)	80%	21.5	11.8	mM	6 mM phosphate buffer	6.1	Room temperature	H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in mM) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten	Km (mM)	Tetrahydrofuran (THF)	90%	21.5	10.8	mM	6 mM phosphate buffer	6.1	Room temperature	H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in mM) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten	kcat/Km	Tetrahydrofuran (THF)	20%	0.72	0.88	deltaA603/(minnmolmM)	6 mM phosphate buffer	6.1	Room temperature	H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(minnmolmM)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten	kcat/Km	Tetrahydrofuran (THF)	30%	0.72	1.05	deltaA603/(minnmolmM)	6 mM phosphate buffer	6.1	Room temperature	H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(minnmolmM)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten	kcat/Km	Tetrahydrofuran (THF)	40%	0.72	1.6	deltaA603/(minnmolmM)	6 mM phosphate buffer	6.1	Room temperature	H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(minnmolmM)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten	kcat/Km	Tetrahydrofuran (THF)	50%	0.72	1.71	deltaA603/(minnmolmM)	6 mM phosphate buffer	6.1	Room temperature	H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(minnmolmM)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten	kcat/Km	Tetrahydrofuran (THF)	60%	0.72	1.46	deltaA603/(minnmolmM)	6 mM phosphate buffer	6.1	Room temperature	H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(minnmolmM)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten	kcat/Km	Tetrahydrofuran (THF)	70%	0.72	0.39	deltaA603/(minnmolmM)	6 mM phosphate buffer	6.1	Room temperature	H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(minnmolmM)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten	kcat/Km	Tetrahydrofuran (THF)	80%	0.72	0.22	deltaA603/(minnmolmM)	6 mM phosphate buffer	6.1	Room temperature	H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(minnmolmM)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten	kcat/Km	Tetrahydrofuran (THF)	90%	0.72	0.34	deltaA603/(minnmolmM)	6 mM phosphate buffer	6.1	Room temperature	H₂O₂ (Hydrogen Peroxide), Pinacyanol chloride	H2O, Oxidized pinacyanol	—	—	Classical aqueous control (in deltaA603/(minnmolmM)) but in the presence of 10% THF \| Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Activity — Michaelis-Menten