Ξ±-amylase (gene amySTU) from Alteromonas macleodii B7

Enzyme Description

Species
Alteromonas macleodii B7 β†—
Extremophile
No but"cold-adapted" enzyme
EC Number
3.2.1.1 β†—

Sequence

Length: 469 amino acids
MKKHAAGVLIGAALSTLSYQSYADVILHAFNWTYDDVAAKAQEIADLGYTKVLVSPAYKSTGDQWWARYQPQDYRVIDNPLGDTADFQAMVNALNAKGVETYADIVFNHMANEAWKRSDLNYPGSEVLQQYSSNQGYYNGITLFGDVSSGLFGGGDFHGTYDQGGPKCISNYSDVGDVQYNRLCGAAPDPGLPDLDPNNWVVAQQKAYLQALKDMGVTGFRVDAAKHMTNYHINAVFDSNIKNGVHVFGEIITTGGAGSSEYDNFLAPYLSATGHSAYDFPLFAQIRSAFSFGGSMNALVDPGAYGQALSGDKAITFSITHDIPLNDGFRYQLLDPTDEHLANAYVLGRDGGVPLLYSDNNESGDNRWVDLYKRDDIKGMVKFHNGVQGSGMQVVSHNDCVIFFKRDHQGVVGINKCADGQDIWVDTSTDNLWWYRNYRDTLSNDVQYITTQWHKFYVPGRSARMWLME
Xuefeng Han et al. (2014) β€” Gene Cloning and Characterization of an Ξ±-Amylase from Alteromonas macleodii B7 for Enteromorpha Polysaccharide Degradation
Journal of Microbiology and Biotechnology  Β· doi:10.4014/jmb.1304.04036 β†—  Β· Stability - Incubation
26 measurements ▼
Database ID
UniParc: UPI0001AEBF4B β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (26 measurements)

26 measurements β€” page 2 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Isopropanol 20% 100.0 99.2 % Incubation: 20 mM Tris-HCl buffer, Assay: 20 mM phosphate buffer Incubation: 7.5, Assay: 7 Incubation: 25Β°C, Assay: 40Β°C 0.5% Starch Reducing sugars β€” Incubation: 150 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncerainty about control
Stability - Incubation Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Formaldehyde 20% 100.0 9.6 % Incubation: 20 mM Tris-HCl buffer, Assay: 20 mM phosphate buffer Incubation: 7.5, Assay: 7 Incubation: 25Β°C, Assay: 40Β°C 0.5% Starch Reducing sugars β€” Incubation: 150 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncerainty about control
Stability - Incubation Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Acetone 20% 100.0 104.7 % Incubation: 20 mM Tris-HCl buffer, Assay: 20 mM phosphate buffer Incubation: 7.5, Assay: 7 Incubation: 25Β°C, Assay: 40Β°C 0.5% Starch Reducing sugars β€” Incubation: 150 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncerainty about control
Stability - Incubation Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Ethanol 20% 100.0 109.1 % Incubation: 20 mM Tris-HCl buffer, Assay: 20 mM phosphate buffer Incubation: 7.5, Assay: 7 Incubation: 25Β°C, Assay: 40Β°C 0.5% Starch Reducing sugars β€” Incubation: 150 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncerainty about control
Stability - Incubation Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Acetonitrile 20% 100.0 37.3 % Incubation: 20 mM Tris-HCl buffer, Assay: 20 mM phosphate buffer Incubation: 7.5, Assay: 7 Incubation: 25Β°C, Assay: 40Β°C 0.5% Starch Reducing sugars β€” Incubation: 150 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncerainty about control
Stability - Incubation Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Methanol 20% 100.0 121.3 % Incubation: 20 mM Tris-HCl buffer, Assay: 20 mM phosphate buffer Incubation: 7.5, Assay: 7 Incubation: 25Β°C, Assay: 40Β°C 0.5% Starch Reducing sugars β€” Incubation: 150 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncerainty about control
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Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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