Ξ±-amylase (gene amySTU) from Alteromonas macleodii B7
Enzyme Description
Sequence
MKKHAAGVLIGAALSTLSYQSYADVILHAFNWTYDDVAAKAQEIADLGYTKVLVSPAYKSTGDQWWARYQPQDYRVIDNPLGDTADFQAMVNALNAKGVETYADIVFNHMANEAWKRSDLNYPGSEVLQQYSSNQGYYNGITLFGDVSSGLFGGGDFHGTYDQGGPKCISNYSDVGDVQYNRLCGAAPDPGLPDLDPNNWVVAQQKAYLQALKDMGVTGFRVDAAKHMTNYHINAVFDSNIKNGVHVFGEIITTGGAGSSEYDNFLAPYLSATGHSAYDFPLFAQIRSAFSFGGSMNALVDPGAYGQALSGDKAITFSITHDIPLNDGFRYQLLDPTDEHLANAYVLGRDGGVPLLYSDNNESGDNRWVDLYKRDDIKGMVKFHNGVQGSGMQVVSHNDCVIFFKRDHQGVVGINKCADGQDIWVDTSTDNLWWYRNYRDTLSNDVQYITTQWHKFYVPGRSARMWLME
Xuefeng Han et al. (2014)
β Gene Cloning and Characterization of an Ξ±-Amylase from Alteromonas macleodii B7 for Enteromorpha Polysaccharide Degradation
Journal of Microbiology and Biotechnology
Β· doi:10.4014/jmb.1304.04036 β
Β· Stability - Incubation
▼
Database ID
UniParc: UPI0001AEBF4B β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (26 measurements)
26 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Dimethylformamide (DMF) | 50% | 100.0 | 81.8 | % | Incubation: 20 mM Tris-HCl buffer, Assay: 20 mM phosphate buffer | Incubation: 7.5, Assay: 7 | Incubation: 25Β°C, Assay: 40Β°C | 0.5% Starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncerainty about control |
| Stability - Incubation | Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | n-Hexane | 50% | 100.0 | 135.8 | % | Incubation: 20 mM Tris-HCl buffer, Assay: 20 mM phosphate buffer | Incubation: 7.5, Assay: 7 | Incubation: 25Β°C, Assay: 40Β°C | 0.5% Starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncerainty about control |
| Stability - Incubation | Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Xylene | 50% | 100.0 | 17.3 | % | Incubation: 20 mM Tris-HCl buffer, Assay: 20 mM phosphate buffer | Incubation: 7.5, Assay: 7 | Incubation: 25Β°C, Assay: 40Β°C | 0.5% Starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncerainty about control |
| Stability - Incubation | Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Toluene | 50% | 100.0 | 39.6 | % | Incubation: 20 mM Tris-HCl buffer, Assay: 20 mM phosphate buffer | Incubation: 7.5, Assay: 7 | Incubation: 25Β°C, Assay: 40Β°C | 0.5% Starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncerainty about control |
| Stability - Incubation | Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Chloroform | 50% | 100.0 | 20.2 | % | Incubation: 20 mM Tris-HCl buffer, Assay: 20 mM phosphate buffer | Incubation: 7.5, Assay: 7 | Incubation: 25Β°C, Assay: 40Β°C | 0.5% Starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncerainty about control |
| Stability - Incubation | Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Isoamyl Alcohol | 50% | 100.0 | 112.6 | % | Incubation: 20 mM Tris-HCl buffer, Assay: 20 mM phosphate buffer | Incubation: 7.5, Assay: 7 | Incubation: 25Β°C, Assay: 40Β°C | 0.5% Starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncerainty about control |
| Stability - Incubation | Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | 1-Butanol | 50% | 100.0 | 79.0 | % | Incubation: 20 mM Tris-HCl buffer, Assay: 20 mM phosphate buffer | Incubation: 7.5, Assay: 7 | Incubation: 25Β°C, Assay: 40Β°C | 0.5% Starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncerainty about control |
| Stability - Incubation | Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Isopropanol | 50% | 100.0 | 96.0 | % | Incubation: 20 mM Tris-HCl buffer, Assay: 20 mM phosphate buffer | Incubation: 7.5, Assay: 7 | Incubation: 25Β°C, Assay: 40Β°C | 0.5% Starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncerainty about control |
| Stability - Incubation | Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Formaldehyde | 50% | 100.0 | 18.0 | % | Incubation: 20 mM Tris-HCl buffer, Assay: 20 mM phosphate buffer | Incubation: 7.5, Assay: 7 | Incubation: 25Β°C, Assay: 40Β°C | 0.5% Starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncerainty about control |
| Stability - Incubation | Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Acetone | 50% | 100.0 | 82.5 | % | Incubation: 20 mM Tris-HCl buffer, Assay: 20 mM phosphate buffer | Incubation: 7.5, Assay: 7 | Incubation: 25Β°C, Assay: 40Β°C | 0.5% Starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncerainty about control |
| Stability - Incubation | Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Ethanol | 50% | 100.0 | 109.8 | % | Incubation: 20 mM Tris-HCl buffer, Assay: 20 mM phosphate buffer | Incubation: 7.5, Assay: 7 | Incubation: 25Β°C, Assay: 40Β°C | 0.5% Starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncerainty about control |
| Stability - Incubation | Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Acetonitrile | 50% | 100.0 | 6.87 | % | Incubation: 20 mM Tris-HCl buffer, Assay: 20 mM phosphate buffer | Incubation: 7.5, Assay: 7 | Incubation: 25Β°C, Assay: 40Β°C | 0.5% Starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncerainty about control |
| Stability - Incubation | Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Methanol | 50% | 100.0 | 123.3 | % | Incubation: 20 mM Tris-HCl buffer, Assay: 20 mM phosphate buffer | Incubation: 7.5, Assay: 7 | Incubation: 25Β°C, Assay: 40Β°C | 0.5% Starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncerainty about control |
| Stability - Incubation | Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Dimethylformamide (DMF) | 20% | 100.0 | 95.0 | % | Incubation: 20 mM Tris-HCl buffer, Assay: 20 mM phosphate buffer | Incubation: 7.5, Assay: 7 | Incubation: 25Β°C, Assay: 40Β°C | 0.5% Starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncerainty about control |
| Stability - Incubation | Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | n-Hexane | 20% | 100.0 | 124.4 | % | Incubation: 20 mM Tris-HCl buffer, Assay: 20 mM phosphate buffer | Incubation: 7.5, Assay: 7 | Incubation: 25Β°C, Assay: 40Β°C | 0.5% Starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncerainty about control |
| Stability - Incubation | Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Xylene | 20% | 100.0 | 112.5 | % | Incubation: 20 mM Tris-HCl buffer, Assay: 20 mM phosphate buffer | Incubation: 7.5, Assay: 7 | Incubation: 25Β°C, Assay: 40Β°C | 0.5% Starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncerainty about control |
| Stability - Incubation | Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Toluene | 20% | 100.0 | 90.4 | % | Incubation: 20 mM Tris-HCl buffer, Assay: 20 mM phosphate buffer | Incubation: 7.5, Assay: 7 | Incubation: 25Β°C, Assay: 40Β°C | 0.5% Starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncerainty about control |
| Stability - Incubation | Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Chloroform | 20% | 100.0 | 68.6 | % | Incubation: 20 mM Tris-HCl buffer, Assay: 20 mM phosphate buffer | Incubation: 7.5, Assay: 7 | Incubation: 25Β°C, Assay: 40Β°C | 0.5% Starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncerainty about control |
| Stability - Incubation | Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Isoamyl Alcohol | 20% | 100.0 | 118.3 | % | Incubation: 20 mM Tris-HCl buffer, Assay: 20 mM phosphate buffer | Incubation: 7.5, Assay: 7 | Incubation: 25Β°C, Assay: 40Β°C | 0.5% Starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncerainty about control |
| Stability - Incubation | Activity after incubation (2 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | 1-Butanol | 20% | 100.0 | 100.2 | % | Incubation: 20 mM Tris-HCl buffer, Assay: 20 mM phosphate buffer | Incubation: 7.5, Assay: 7 | Incubation: 25Β°C, Assay: 40Β°C | 0.5% Starch | Reducing sugars | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncerainty about control |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.