L-Haloacid Dehalogenase (gene PinHAD) from Psychromonas ingrahamii

Enzyme Description

Species
Psychromonas ingrahamii β†—
Extremophile
Yes "psychorphilic" organism
EC Number
3.8.1.2 β†—

Sequence

Length: 225 amino acids
MSVILAFDVYGTLINTHGIVTILEKWLGKNAQAFSQTWRDKQLEYSFRRGLMQNYQSFAVCTRHALDYTCNFYRVSLSKEQKQALMDGYKTLPAFDDAANALAQLKGAGFKLYAFSNGTKEAVEELLTGAGIRAFFDGIVSCDDLKSFKPNPAVYCHFLRESGAKHNQAWLISSNPFDVIGAISTGMLSAWVQRSQDAIFDPWDVDPTAIITSLKELKGVLPKPD
Halina R. Novak et al. (2013) β€” Characterisation of an l-Haloacid Dehalogenase from the Marine Psychrophile Psychromonas ingrahamii with Potential Industrial Application
Marine Biotechnology  Β· doi:10.1007/s10126-013-9522-3 β†—  Β· Stability - Incubation Stability - Tm
34 measurements ▼
Database ID
β€”
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli ArcticExpressTM (DE3)

Experimental Data (34 measurements)

34 measurements β€” page 2 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase Ethanol 40% 100.0 56.5 % Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 ΞΌg/ml phenol red Incubation: ?, Assay: 8.2 Incubation: Room temperature, Assay: 25Β°C 10 mM Chloroacetic acid Chloride ion , Glycolic Acid β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about control, clear about assay in water
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase Ethanol 30% 100.0 81.2 % Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 ΞΌg/ml phenol red Incubation: ?, Assay: 8.2 Incubation: Room temperature, Assay: 25Β°C 10 mM Chloroacetic acid Chloride ion , Glycolic Acid β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about control, clear about assay in water
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase Ethanol 20% 100.0 95.3 % Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 ΞΌg/ml phenol red Incubation: ?, Assay: 8.2 Incubation: Room temperature, Assay: 25Β°C 10 mM Chloroacetic acid Chloride ion , Glycolic Acid β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about control, clear about assay in water
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase Ethanol 10% 100.0 98.6 % Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 ΞΌg/ml phenol red Incubation: ?, Assay: 8.2 Incubation: Room temperature, Assay: 25Β°C 10 mM Chloroacetic acid Chloride ion , Glycolic Acid β€” β€” Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about control, clear about assay in water
Stability - Tm Melting temperature measured by DSF using using SYPRO Orange dye Ethanol 20% 85.3 57.1 Β°C 5 mM potassium phosphate buffer, ? mM SYPRO Orange dye 7 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C) | Uncertain pH value (usual potassium phosphate buffer pH)
Stability - Tm Melting temperature measured by DSF using using SYPRO Orange dye Ethanol 10% 85.3 70.7 Β°C 5 mM potassium phosphate buffer, ? mM SYPRO Orange dye 7 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C) | Uncertain pH value (usual potassium phosphate buffer pH)
Stability - Tm Melting temperature measured by DSF using using SYPRO Orange dye Dimethyl Sulfoxide (DMSO) 10% 85.3 77.8 Β°C 5 mM potassium phosphate buffer, ? mM SYPRO Orange dye 7 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C) | Uncertain pH value (usual potassium phosphate buffer pH)
Stability - Tm Melting temperature measured by DSF using using SYPRO Orange dye Dimethyl Sulfoxide (DMSO) 20% 85.3 73.3 Β°C 5 mM potassium phosphate buffer, ? mM SYPRO Orange dye 7 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C) | Uncertain pH value (usual potassium phosphate buffer pH)
Stability - Tm Melting temperature measured by DSF using using SYPRO Orange dye Dimethyl Sulfoxide (DMSO) 30% 85.3 67.0 Β°C 5 mM potassium phosphate buffer, ? mM SYPRO Orange dye 7 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C) | Uncertain pH value (usual potassium phosphate buffer pH)
Stability - Tm Melting temperature measured by DSF using using SYPRO Orange dye Acetonitrile 10% 85.3 71.4 Β°C 5 mM potassium phosphate buffer, ? mM SYPRO Orange dye 7 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C) | Uncertain pH value (usual potassium phosphate buffer pH)
Stability - Tm Melting temperature measured by DSF using using SYPRO Orange dye Acetonitrile 20% 85.3 57.1 Β°C 5 mM potassium phosphate buffer, ? mM SYPRO Orange dye 7 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C) | Uncertain pH value (usual potassium phosphate buffer pH)
Stability - Tm Melting temperature measured by DSF using using SYPRO Orange dye Methanol 10% 85.3 75.9 Β°C 5 mM potassium phosphate buffer, ? mM SYPRO Orange dye 7 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C) | Uncertain pH value (usual potassium phosphate buffer pH)
Stability - Tm Melting temperature measured by DSF using using SYPRO Orange dye Methanol 20% 85.3 65.3 Β°C 5 mM potassium phosphate buffer, ? mM SYPRO Orange dye 7 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C) | Uncertain pH value (usual potassium phosphate buffer pH)
Stability - Tm Melting temperature measured by DSF using using SYPRO Orange dye Methanol 30% 85.3 48.2 Β°C 5 mM potassium phosphate buffer, ? mM SYPRO Orange dye 7 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C) | Uncertain pH value (usual potassium phosphate buffer pH)
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Visualization : Stability β€” Tm

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Visualization : Stability β€” Incubation

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Structure

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