L-Haloacid Dehalogenase (gene PinHAD) from Psychromonas ingrahamii

Enzyme Description

Species

Psychromonas ingrahamii ↗

Extremophile

Yes "psychorphilic" organism

EC Number

3.8.1.2 ↗

Sequence

Length: 225 amino acids

MSVILAFDVYGTLINTHGIVTILEKWLGKNAQAFSQTWRDKQLEYSFRRGLMQNYQSFAVCTRHALDYTCNFYRVSLSKEQKQALMDGYKTLPAFDDAANALAQLKGAGFKLYAFSNGTKEAVEELLTGAGIRAFFDGIVSCDDLKSFKPNPAVYCHFLRESGAKHNQAWLISSNPFDVIGAISTGMLSAWVQRSQDAIFDPWDVDPTAIITSLKELKGVLPKPD

Halina R. Novak et al. (2013) — Characterisation of an l-Haloacid Dehalogenase from the Marine Psychrophile Psychromonas ingrahamii with Potential Industrial Application

Marine Biotechnology · doi:10.1007/s10126-013-9522-3 ↗ · Stability - Incubation Stability - Tm

34 measurements ▼

Database ID

—

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli ArcticExpressTM (DE3)

Experimental Data (34 measurements)

34 measurements — page 1 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100.0	11.1	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion , Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Methanol	30%	100.0	95.1	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion , Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Methanol	40%	100.0	94.6	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion , Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Methanol	50%	100.0	20.5	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion , Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Methanol	60%	100.0	6.5	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion , Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	10%	100.0	94.6	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion , Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	20%	100.0	92.1	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion , Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	30%	100.0	88.4	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion , Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	40%	100.0	74.2	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion , Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Methanol	20%	100.0	95.1	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion , Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	60%	100.0	6.5	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion , Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Acetonitrile	10%	100.0	64.6	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion , Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Acetonitrile	20%	100.0	51.6	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion , Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Acetonitrile	30%	100.0	37.0	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion , Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Acetonitrile	40%	100.0	31.4	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion , Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Acetonitrile	50%	100.0	13.2	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion , Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Acetonitrile	60%	100.0	6.7	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion , Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Methanol	10%	100.0	93.5	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion , Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Ethanol	60%	100.0	7.0	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion , Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Ethanol	50%	100.0	12.3	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion , Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water

Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100.0	11.1	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion, Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Methanol	30%	100.0	95.1	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion, Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Methanol	40%	100.0	94.6	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion, Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Methanol	50%	100.0	20.5	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion, Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Methanol	60%	100.0	6.5	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion, Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	10%	100.0	94.6	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion, Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	20%	100.0	92.1	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion, Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	30%	100.0	88.4	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion, Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	40%	100.0	74.2	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion, Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Methanol	20%	100.0	95.1	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion, Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	60%	100.0	6.5	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion, Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Acetonitrile	10%	100.0	64.6	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion, Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Acetonitrile	20%	100.0	51.6	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion, Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Acetonitrile	30%	100.0	37.0	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion, Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Acetonitrile	40%	100.0	31.4	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion, Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Acetonitrile	50%	100.0	13.2	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion, Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Acetonitrile	60%	100.0	6.7	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion, Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Methanol	10%	100.0	93.5	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion, Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Ethanol	60%	100.0	7.0	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion, Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Ethanol	50%	100.0	12.3	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion, Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Ethanol	40%	100.0	56.5	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion, Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Ethanol	30%	100.0	81.2	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion, Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Ethanol	20%	100.0	95.3	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion, Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (decrease in phenol red absorbance measurement, 540 nm) in aqueous phase	Ethanol	10%	100.0	98.6	%	Incubation: ?, Assay: 1 mM HEPES buffer, 1 mM EDTA, 20 mM sodium sulphate, 20 μg/ml phenol red	Incubation: ?, Assay: 8.2	Incubation: Room temperature, Assay: 25°C	10 mM Chloroacetic acid	Chloride ion, Glycolic Acid	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about control, clear about assay in water
Stability - Tm	Melting temperature measured by DSF using using SYPRO Orange dye	Ethanol	20%	85.3	57.1	°C	5 mM potassium phosphate buffer, ? mM SYPRO Orange dye	7	—	—	—	—	—	Classical aqueous control (in °C) \| Uncertain pH value (usual potassium phosphate buffer pH)
Stability - Tm	Melting temperature measured by DSF using using SYPRO Orange dye	Ethanol	10%	85.3	70.7	°C	5 mM potassium phosphate buffer, ? mM SYPRO Orange dye	7	—	—	—	—	—	Classical aqueous control (in °C) \| Uncertain pH value (usual potassium phosphate buffer pH)
Stability - Tm	Melting temperature measured by DSF using using SYPRO Orange dye	Dimethyl Sulfoxide (DMSO)	10%	85.3	77.8	°C	5 mM potassium phosphate buffer, ? mM SYPRO Orange dye	7	—	—	—	—	—	Classical aqueous control (in °C) \| Uncertain pH value (usual potassium phosphate buffer pH)
Stability - Tm	Melting temperature measured by DSF using using SYPRO Orange dye	Dimethyl Sulfoxide (DMSO)	20%	85.3	73.3	°C	5 mM potassium phosphate buffer, ? mM SYPRO Orange dye	7	—	—	—	—	—	Classical aqueous control (in °C) \| Uncertain pH value (usual potassium phosphate buffer pH)
Stability - Tm	Melting temperature measured by DSF using using SYPRO Orange dye	Dimethyl Sulfoxide (DMSO)	30%	85.3	67.0	°C	5 mM potassium phosphate buffer, ? mM SYPRO Orange dye	7	—	—	—	—	—	Classical aqueous control (in °C) \| Uncertain pH value (usual potassium phosphate buffer pH)
Stability - Tm	Melting temperature measured by DSF using using SYPRO Orange dye	Acetonitrile	10%	85.3	71.4	°C	5 mM potassium phosphate buffer, ? mM SYPRO Orange dye	7	—	—	—	—	—	Classical aqueous control (in °C) \| Uncertain pH value (usual potassium phosphate buffer pH)
Stability - Tm	Melting temperature measured by DSF using using SYPRO Orange dye	Acetonitrile	20%	85.3	57.1	°C	5 mM potassium phosphate buffer, ? mM SYPRO Orange dye	7	—	—	—	—	—	Classical aqueous control (in °C) \| Uncertain pH value (usual potassium phosphate buffer pH)
Stability - Tm	Melting temperature measured by DSF using using SYPRO Orange dye	Methanol	10%	85.3	75.9	°C	5 mM potassium phosphate buffer, ? mM SYPRO Orange dye	7	—	—	—	—	—	Classical aqueous control (in °C) \| Uncertain pH value (usual potassium phosphate buffer pH)
Stability - Tm	Melting temperature measured by DSF using using SYPRO Orange dye	Methanol	20%	85.3	65.3	°C	5 mM potassium phosphate buffer, ? mM SYPRO Orange dye	7	—	—	—	—	—	Classical aqueous control (in °C) \| Uncertain pH value (usual potassium phosphate buffer pH)
Stability - Tm	Melting temperature measured by DSF using using SYPRO Orange dye	Methanol	30%	85.3	48.2	°C	5 mM potassium phosphate buffer, ? mM SYPRO Orange dye	7	—	—	—	—	—	Classical aqueous control (in °C) \| Uncertain pH value (usual potassium phosphate buffer pH)

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Visualization : Stability — T_m

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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