Carboxylesterase (gene bioHx) from an aquaculture pond sediment metagenomic library

Enzyme Description

Species

Na (aquaculture pond sediment metagenomic library)

Extremophile

EC Number

3.1.1.1 ↗

Sequence

Length: 259 amino acids

MHIEVTGRGPDLVLIHGWALQGGVFAPLVQRLADQFTLHLVDLPGHGHSREDTTPLRLPFVVNAIAAATPPAVWCGWSMGGLFALHAAATLPKVRGLATIAATPRFVRGEDWPHAVEPAVFEQFGRELATDFGGTLERFLALDVMGSAHAREELRTLRQRLVERXAPTERALLEGLRLLESTDLRGALPTLGKPSLWIAGQRDRLVSPAAMQAAAALAPGAQALTIAHGGHAPFLGHADEVATALQHFVAGLSPAVGGQ

Yuping Shi et al. (2013) — Molecular cloning of a novel bioH gene from an environmental metagenome encoding a carboxylesterase with exceptional tolerance to organic solvents

BMC Biotechnology · doi:10.1186/1472-6750-13-13 ↗ · Stability - Incubation

32 measurements ▼

Database ID

UniParc: UPI0002B8B2EE ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (32 measurements)

32 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	1-Butanol	20%	100.0	48.9	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Isopropanol	50%	100.0	76.2	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Isopropanol	40%	100.0	76.8	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Isopropanol	30%	100.0	86.3	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Isopropanol	20%	100.0	86.3	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Ethanol	50%	100.0	75.4	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Ethanol	40%	100.0	77.9	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Ethanol	30%	100.0	82.2	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Ethanol	20%	100.0	85.5	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Methanol	50%	100.0	57.0	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Methanol	40%	100.0	85.5	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Methanol	30%	100.0	93.6	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase

Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Acetonitrile	20%	100.0	74.4	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Dimethylformamide (DMF)	50%	100.0	19.7	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Dimethylformamide (DMF)	40%	100.0	72.3	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Dimethylformamide (DMF)	30%	100.0	97.3	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Dimethylformamide (DMF)	20%	100.0	97.7	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100.0	29.7	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	40%	100.0	54.2	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	30%	100.0	95.3	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	20%	100.0	95.7	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Acetone	50%	100.0	15.9	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Acetone	40%	100.0	62.1	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Acetone	30%	100.0	78.0	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Acetone	20%	100.0	79.6	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Acetonitrile	50%	100.0	44.3	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Acetonitrile	40%	100.0	65.0	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Acetonitrile	30%	100.0	74.4	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Methanol	20%	100.0	94.6	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	1-Butanol	50%	100.0	23.5	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	1-Butanol	40%	100.0	30.8	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	1-Butanol	30%	100.0	47.9	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	1-Butanol	20%	100.0	48.9	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Isopropanol	50%	100.0	76.2	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Isopropanol	40%	100.0	76.8	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Isopropanol	30%	100.0	86.3	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Isopropanol	20%	100.0	86.3	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Ethanol	50%	100.0	75.4	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Ethanol	40%	100.0	77.9	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Ethanol	30%	100.0	82.2	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Ethanol	20%	100.0	85.5	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Methanol	50%	100.0	57.0	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Methanol	40%	100.0	85.5	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Methanol	30%	100.0	93.6	%	Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase

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Visualization : Stability — Incubation

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