Carboxylesterase (gene bioHx) from an aquaculture pond sediment metagenomic library

Enzyme Description

Species
Na (aquaculture pond sediment metagenomic library)
Extremophile
No
EC Number
3.1.1.1 β†—

Sequence

Length: 259 amino acids
MHIEVTGRGPDLVLIHGWALQGGVFAPLVQRLADQFTLHLVDLPGHGHSREDTTPLRLPFVVNAIAAATPPAVWCGWSMGGLFALHAAATLPKVRGLATIAATPRFVRGEDWPHAVEPAVFEQFGRELATDFGGTLERFLALDVMGSAHAREELRTLRQRLVERXAPTERALLEGLRLLESTDLRGALPTLGKPSLWIAGQRDRLVSPAAMQAAAALAPGAQALTIAHGGHAPFLGHADEVATALQHFVAGLSPAVGGQ
Yuping Shi et al. (2013) β€” Molecular cloning of a novel bioH gene from an environmental metagenome encoding a carboxylesterase with exceptional tolerance to organic solvents
BMC Biotechnology  Β· doi:10.1186/1472-6750-13-13 β†—  Β· Stability - Incubation
32 measurements ▼
Database ID
UniParc: UPI0002B8B2EE β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (32 measurements)

32 measurements β€” page 1 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase Acetonitrile 20% 100.0 74.4 % Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 10 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase Dimethylformamide (DMF) 50% 100.0 19.7 % Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 10 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase Dimethylformamide (DMF) 40% 100.0 72.3 % Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 10 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase Dimethylformamide (DMF) 30% 100.0 97.3 % Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 10 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase Dimethylformamide (DMF) 20% 100.0 97.7 % Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 10 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 50% 100.0 29.7 % Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 10 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 40% 100.0 54.2 % Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 10 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 30% 100.0 95.3 % Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 10 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 20% 100.0 95.7 % Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 10 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase Acetone 50% 100.0 15.9 % Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 10 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase Acetone 40% 100.0 62.1 % Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 10 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase Acetone 30% 100.0 78.0 % Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 10 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase Acetone 20% 100.0 79.6 % Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 10 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase Acetonitrile 50% 100.0 44.3 % Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 10 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase Acetonitrile 40% 100.0 65.0 % Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 10 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase Acetonitrile 30% 100.0 74.4 % Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 10 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase Methanol 20% 100.0 94.6 % Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 10 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase 1-Butanol 50% 100.0 23.5 % Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 10 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase 1-Butanol 40% 100.0 30.8 % Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 10 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase 1-Butanol 30% 100.0 47.9 % Incubation: ?, Assay: 50 mM Tris-HCl, 5% Ethanol Incubation: ?, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 10 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), activity measured in aqueous phase
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Visualization : Stability β€” Incubation

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Structure

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