Aldehyde oxidase 1 from Oryctolagus cuniculus (rabbit)
Enzyme Description
Sequence
Length: 1334 amino acids
MEPAPELLFYVNGRKVVEKQVDPETMLLPYLRKKLRLTGTKYGCGGGGCGACTVMISRYNRVTKKIRHYPVNACLTPICSLYGAAVTTVEGIGSTTTRLHPVQERIAKFHGTQCGFCTPGMVMSMYALLRNHPEPTLDQLADALGGNLCRCTGYRPIIEAYKTFCKTSDCCQNKENGFCCLDQGINGLPEVEEENQTRPNLFSEEEYLPLDPTQELIFPPELMTMAEKQPQRTRVFSGERMMWISPVTLKALLEAKSTYPQAPVVMGNTSVGPGVKFKGIFHPVIISPDSIEELNVVSHTHSGLTLGAGLSLAQVKDILADVVQKVPEENAQTYRALLKHLGTLAGSQIRNMASLGGHIISRHLDSDLNPLLAVGNCTLNVLSKEGERQIPLDEQFLSRCPEADLKPQEILASVHIPYSRKWEFVLAFRQAQRKQNALAIVNSGMRVFFGEGDGIIRELAISYGGVGPTIICAKNSCQKLIGRSWNEEMLDTACRLILDEVSLPGSAPGGKVEFKRTLIISFLFKFYLEVSQILKRMAPGLSPHLADKYESALQDLHARYSWSTLKDQDVDARQLSQDPIGHPVMHLSGVKHATGEAIYLDDMPAVDQELFMAFVTSPRAHAKIVSTDLLEALSLPGVVDIVTAEHLQDGNTFYTEKLLAADEVLCVGQLVCAVIAESEVQAKQAAKQVKIVYEDLEPVILSIEEAIEQKSFFEPERKLEYGNVDEAFKVVDQILEGEIHMGGQEHFYMETQSVLVVPKGEDQEMDVYASTQFPKYIQDMVAAVLKLPVNKVMCHVKRVGGAFGGKVFKASIMAAIAAFAANKHGRAVRCILERGEDMLITGGRHPYLGKYKAGFMNDGRIVALDVEHYSNGGCSLDESLLVIEMGLLKMENAYKFPNLRCRGWACRTNLPSNTAFRGFGFPQAGLITECCITEVAAKCGLSPEKVRAINFYKEIDQTPYKQEINAKNLTQCWNECLAKSSYFQRKVAVEKFNAENYWKQRGLAIIPFKYPRGLGSVAYGQAAALVHVYLDGSVLVTHGGIEMGQGVHTKMIQVVSRELKMPMSNVHLRGTSTETVPNTNASGGSVVADLNGLAVKDACQTLLKRLEPIINKNPQGTWKEWAQAAFDKSISLSATGYFRGYDSNIDWDKGEGHPFEYFVYGAACSEVEIDCLTGDHKTIRTDIVMDVGYSINPALDIGQVEGAFIQGMGLYTIEELHYSPQGILYSRGPNQYKIPAICDIPAELNVTFLPPSEKSNTLYSSKGLGESGVFMGCSVFFAIREAVCAARQARGLSAPWKLSSPLTPEKIRMACEDKFTKMIPRDKPGSYVPWNVPV
Mohammad-Reza Rashidi et al. (2013)
β Aldehyde Oxidase Activity and Stability in Water-Miscible Organic Solvents
Applied Biochemistry and Biotechnology
Β· doi:10.1007/s12010-012-0036-7 β
Β· Activity - Classical Activity - Michaelis-Menten Stability - C50 Stability - Incubation
243 measurements
▼
Database ID
UniProt: P80456 β
Sequence Annotation
Inferred - from protein name
Protein Source
Purified, eukaryote Oryctolagus cuniculus (rabbit)
Experimental Data (243 measurements)
243 measurements β page 13 of 13
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (144 minutes at 35Β°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase | Methanol | 0.5% | 100.0 | 52.0 | % | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | Incubation: 7.4, Assay: 4 | Incubation: 35Β°C, Assay: 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Water-incubated control (in %), activity measured in aqueous phase |
| Stability - Incubation | Activity after incubation (144 minutes at 35Β°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase | 1,4-Dioxane | 6% | 100.0 | 52.0 | % | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | Incubation: 7.4, Assay: 4 | Incubation: 35Β°C, Assay: 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Water-incubated control (in %), activity measured in aqueous phase |
| Stability - Incubation | Activity after incubation (144 minutes at 35Β°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase | Acetonitrile | 5% | 100.0 | 54.0 | % | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | Incubation: 7.4, Assay: 4 | Incubation: 35Β°C, Assay: 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Water-incubated control (in %), activity measured in aqueous phase |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Tetrahydrofuran (THF) | 0.5% | 100.0 | 56.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Tetrahydrofuran (THF) | 1% | 100.0 | 42.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Tetrahydrofuran (THF) | 1.5% | 100.0 | 35.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Tetrahydrofuran (THF) | 2% | 100.0 | 30.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Tetrahydrofuran (THF) | 2.5% | 100.0 | 23.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Tetrahydrofuran (THF) | 5% | 100.0 | 10.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Tetrahydrofuran (THF) | 10% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Tetrahydrofuran (THF) | 15% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Tetrahydrofuran (THF) | 20% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Tetrahydrofuran (THF) | 25% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Tetrahydrofuran (THF) | 30% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Tetrahydrofuran (THF) | 35% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 0.5% | 100.0 | 98.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 1% | 100.0 | 94.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 1.5% | 100.0 | 90.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 2% | 100.0 | 87.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 2.5% | 100.0 | 82.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 5% | 100.0 | 73.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 10% | 100.0 | 63.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 15% | 100.0 | 48.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 20% | 100.0 | 39.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 25% | 100.0 | 25.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 30% | 100.0 | 10.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 35% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1-Propanol | 0.5% | 100.0 | 69.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1-Propanol | 1% | 100.0 | 60.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1-Propanol | 1.5% | 100.0 | 54.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1-Propanol | 2% | 100.0 | 49.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1-Propanol | 2.5% | 100.0 | 40.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1-Propanol | 5% | 100.0 | 30.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1-Propanol | 10% | 100.0 | 17.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1-Propanol | 15% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1-Propanol | 20% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1-Propanol | 25% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1-Propanol | 30% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1-Propanol | 35% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Isopropanol | 0.5% | 100.0 | 80.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Isopropanol | 1% | 100.0 | 74.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Isopropanol | 1.5% | 100.0 | 70.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Isopropanol | 2% | 100.0 | 65.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Isopropanol | 2.5% | 100.0 | 56.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Isopropanol | 5% | 100.0 | 43.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Isopropanol | 10% | 100.0 | 26.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Isopropanol | 15% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Isopropanol | 20% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Isopropanol | 25% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Isopropanol | 30% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Isopropanol | 35% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1,4-Dioxane | 0.5% | 100.0 | 95.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1,4-Dioxane | 1% | 100.0 | 90.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1,4-Dioxane | 1.5% | 100.0 | 85.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1,4-Dioxane | 2% | 100.0 | 78.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1,4-Dioxane | 2.5% | 100.0 | 70.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1,4-Dioxane | 5% | 100.0 | 60.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1,4-Dioxane | 10% | 100.0 | 43.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1,4-Dioxane | 15% | 100.0 | 28.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1,4-Dioxane | 20% | 100.0 | 13.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1,4-Dioxane | 25% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1,4-Dioxane | 30% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1,4-Dioxane | 35% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Pyridine | 0.5% | 100.0 | 40.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Pyridine | 1% | 100.0 | 22.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Pyridine | 1.5% | 100.0 | 17.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Pyridine | 2% | 100.0 | 13.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Pyridine | 2.5% | 100.0 | 5.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Pyridine | 5% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Pyridine | 10% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Pyridine | 15% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Pyridine | 20% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Pyridine | 25% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Pyridine | 30% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Pyridine | 35% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Methanol | 0.5% | 100.0 | 65.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Methanol | 1% | 100.0 | 45.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Methanol | 1.5% | 100.0 | 38.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Methanol | 2% | 100.0 | 32.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Methanol | 2.5% | 100.0 | 26.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Methanol | 5% | 100.0 | 15.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Methanol | 10% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Methanol | 15% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Methanol | 20% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Methanol | 25% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Methanol | 30% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Methanol | 35% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Acetonitrile | 0.5% | 100.0 | 95.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Acetonitrile | 1% | 100.0 | 88.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Acetonitrile | 1.5% | 100.0 | 83.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Acetonitrile | 2% | 100.0 | 79.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Acetonitrile | 2.5% | 100.0 | 72.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Acetonitrile | 5% | 100.0 | 63.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Acetonitrile | 10% | 100.0 | 41.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Acetonitrile | 15% | 100.0 | 17.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Acetonitrile | 20% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Acetonitrile | 25% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Acetonitrile | 30% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Acetonitrile | 35% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Ethanol | 0.5% | 100.0 | 98.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Ethanol | 1% | 100.0 | 93.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Ethanol | 1.5% | 100.0 | 88.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Ethanol | 2% | 100.0 | 83.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Ethanol | 2.5% | 100.0 | 77.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Ethanol | 5% | 100.0 | 68.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Ethanol | 10% | 100.0 | 55.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Ethanol | 15% | 100.0 | 42.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Ethanol | 20% | 100.0 | 33.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Ethanol | 25% | 100.0 | 20.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Ethanol | 30% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Ethanol | 35% | 100.0 | 0.0 | % | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Tetrahydrofuran (THF) | 0.5% | 57.0 | 28.0 | Β΅M/mg product/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 25Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Tetrahydrofuran (THF) | 0.5% | 71.0 | 49.0 | Β΅M/mg product/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Tetrahydrofuran (THF) | 0.5% | 94.0 | 65.0 | Β΅M/mg product/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 45Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 6% | 57.0 | 37.0 | Β΅M/mg product/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 25Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 6% | 71.0 | 50.0 | Β΅M/mg product/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 6% | 94.0 | 73.0 | Β΅M/mg product/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 45Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1-Propanol | 1% | 57.0 | 21.0 | Β΅M/mg product/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 25Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1-Propanol | 1% | 71.0 | 37.0 | Β΅M/mg product/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1-Propanol | 1% | 94.0 | 57.0 | Β΅M/mg product/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 45Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Isopropanol | 2% | 57.0 | 22.0 | Β΅M/mg product/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 25Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Isopropanol | 2% | 71.0 | 34.0 | Β΅M/mg product/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Isopropanol | 2% | 94.0 | 73.0 | Β΅M/mg product/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 45Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1,4-Dioxane | 6% | 57.0 | 20.0 | Β΅M/mg product/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 25Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1,4-Dioxane | 6% | 71.0 | 28.0 | Β΅M/mg product/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1,4-Dioxane | 6% | 94.0 | 33.0 | Β΅M/mg product/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 45Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Pyridine | 0.5% | 57.0 | 13.0 | Β΅M/mg product/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 25Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Pyridine | 0.5% | 71.0 | 25.0 | Β΅M/mg product/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Pyridine | 0.5% | 94.0 | 33.0 | Β΅M/mg product/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 45Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Methanol | 0.5% | 57.0 | 35.0 | Β΅M/mg product/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 25Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Methanol | 0.5% | 71.0 | 50.0 | Β΅M/mg product/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Methanol | 0.5% | 94.0 | 82.0 | Β΅M/mg product/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 45Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Acetonitrile | 5% | 57.0 | 21.0 | Β΅M/mg product/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 25Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Acetonitrile | 5% | 71.0 | 48.0 | Β΅M/mg product/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Acetonitrile | 5% | 94.0 | 67.0 | Β΅M/mg product/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 45Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Ethanol | 5% | 57.0 | 30.0 | Β΅M/mg product/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 25Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Ethanol | 5% | 71.0 | 45.0 | Β΅M/mg product/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Ethanol | 5% | 94.0 | 67.0 | Β΅M/mg product/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 45Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity (in Β΅M/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement) | Tetrahydrofuran (THF) | 0.5% | 17.0 | 10.0 | Β΅M/mg product/min | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 6 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity (in Β΅M/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement) | Tetrahydrofuran (THF) | 0.5% | 50.0 | 30.0 | Β΅M/mg product/min | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity (in Β΅M/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement) | Tetrahydrofuran (THF) | 0.5% | 51.0 | 34.0 | Β΅M/mg product/min | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 8 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity (in Β΅M/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement) | Tetrahydrofuran (THF) | 0.5% | 63.0 | 36.0 | Β΅M/mg product/min | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 9 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity (in Β΅M/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement) | Dimethylformamide (DMF) | 6% | 17.0 | 10.0 | Β΅M/mg product/min | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 6 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity (in Β΅M/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement) | Dimethylformamide (DMF) | 6% | 50.0 | 21.0 | Β΅M/mg product/min | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity (in Β΅M/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement) | Dimethylformamide (DMF) | 6% | 51.0 | 39.0 | Β΅M/mg product/min | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 8 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity (in Β΅M/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement) | Dimethylformamide (DMF) | 6% | 63.0 | 43.0 | Β΅M/mg product/min | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 9 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity (in Β΅M/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement) | 1-Propanol | 1% | 17.0 | 11.0 | Β΅M/mg product/min | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 6 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity (in Β΅M/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement) | 1-Propanol | 1% | 50.0 | 28.0 | Β΅M/mg product/min | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity (in Β΅M/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement) | 1-Propanol | 1% | 51.0 | 36.0 | Β΅M/mg product/min | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 8 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity (in Β΅M/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement) | 1-Propanol | 1% | 63.0 | 22.0 | Β΅M/mg product/min | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 9 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity (in Β΅M/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement) | Isopropanol | 2% | 17.0 | 7.0 | Β΅M/mg product/min | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 6 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity (in Β΅M/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement) | Isopropanol | 2% | 50.0 | 21.0 | Β΅M/mg product/min | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity (in Β΅M/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement) | Isopropanol | 2% | 51.0 | 28.0 | Β΅M/mg product/min | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 8 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity (in Β΅M/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement) | Isopropanol | 2% | 63.0 | 35.0 | Β΅M/mg product/min | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 9 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity (in Β΅M/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement) | 1,4-Dioxane | 6% | 17.0 | 10.0 | Β΅M/mg product/min | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 6 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity (in Β΅M/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement) | 1,4-Dioxane | 6% | 50.0 | 28.0 | Β΅M/mg product/min | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity (in Β΅M/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement) | 1,4-Dioxane | 6% | 51.0 | 30.0 | Β΅M/mg product/min | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 8 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity (in Β΅M/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement) | 1,4-Dioxane | 6% | 63.0 | 28.0 | Β΅M/mg product/min | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 9 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity (in Β΅M/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement) | Pyridine | 0.5% | 17.0 | 8.0 | Β΅M/mg product/min | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 6 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity (in Β΅M/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement) | Pyridine | 0.5% | 50.0 | 18.0 | Β΅M/mg product/min | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity (in Β΅M/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement) | Pyridine | 0.5% | 51.0 | 27.0 | Β΅M/mg product/min | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 8 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity (in Β΅M/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement) | Pyridine | 0.5% | 63.0 | 27.0 | Β΅M/mg product/min | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 9 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity (in Β΅M/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement) | Methanol | 0.5% | 17.0 | 11.0 | Β΅M/mg product/min | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 6 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity (in Β΅M/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement) | Methanol | 0.5% | 50.0 | 29.0 | Β΅M/mg product/min | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity (in Β΅M/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement) | Methanol | 0.5% | 51.0 | 42.0 | Β΅M/mg product/min | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 8 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity (in Β΅M/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement) | Methanol | 0.5% | 63.0 | 35.0 | Β΅M/mg product/min | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 9 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity (in Β΅M/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement) | Acetonitrile | 5% | 17.0 | 3.0 | Β΅M/mg product/min | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 6 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity (in Β΅M/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement) | Acetonitrile | 5% | 50.0 | 32.0 | Β΅M/mg product/min | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity (in Β΅M/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement) | Acetonitrile | 5% | 51.0 | 29.0 | Β΅M/mg product/min | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 8 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity (in Β΅M/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement) | Acetonitrile | 5% | 63.0 | 27.0 | Β΅M/mg product/min | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 9 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity (in Β΅M/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement) | Ethanol | 5% | 17.0 | 11.0 | Β΅M/mg product/min | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 6 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity (in Β΅M/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement) | Ethanol | 5% | 50.0 | 26.0 | Β΅M/mg product/min | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity (in Β΅M/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement) | Ethanol | 5% | 51.0 | 34.0 | Β΅M/mg product/min | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 8 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Classical | Activity (in Β΅M/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement) | Ethanol | 5% | 63.0 | 28.0 | Β΅M/mg product/min | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 9 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/mg product/min) |
| Activity - Michaelis-Menten | Km (in Β΅M), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Methanol | 0.5% | 11.0 | 5.0 | Β΅M | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M), activity measured in aqueous phase |
| Activity - Michaelis-Menten | Km (in Β΅M), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Isopropanol | 2% | 11.0 | 7.69 | Β΅M | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M), activity measured in aqueous phase |
| Activity - Michaelis-Menten | Km (in Β΅M), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Acetonitrile | 5% | 11.0 | 6.3 | Β΅M | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M), activity measured in aqueous phase |
| Activity - Michaelis-Menten | Km (in Β΅M), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1-Propanol | 1% | 11.0 | 8.33 | Β΅M | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M), activity measured in aqueous phase |
| Activity - Michaelis-Menten | Km (in Β΅M), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 6% | 11.0 | 10.0 | Β΅M | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M), activity measured in aqueous phase |
| Activity - Michaelis-Menten | Km (in Β΅M), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1,4-Dioxane | 6% | 11.0 | 6.66 | Β΅M | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M), activity measured in aqueous phase |
| Activity - Michaelis-Menten | Km (in Β΅M), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Ethanol | 5% | 11.0 | 8.33 | Β΅M | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M), activity measured in aqueous phase |
| Activity - Michaelis-Menten | Km (in Β΅M), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Tetrahydrofuran (THF) | 0.5% | 11.0 | 11.0 | Β΅M | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M), activity measured in aqueous phase |
| Activity - Michaelis-Menten | Km (in Β΅M), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Pyridine | 0.5% | 11.0 | 6.25 | Β΅M | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M), activity measured in aqueous phase |
| Activity - Michaelis-Menten | Vmax (in Β΅M/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Methanol | 0.5% | 0.039 | 0.018 | Β΅M/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/min), activity measured in aqueous phase |
| Activity - Michaelis-Menten | Vmax (in Β΅M/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Isopropanol | 2% | 0.039 | 0.02 | Β΅M/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/min), activity measured in aqueous phase |
| Activity - Michaelis-Menten | Vmax (in Β΅M/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Acetonitrile | 5% | 0.039 | 0.013 | Β΅M/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/min), activity measured in aqueous phase |
| Activity - Michaelis-Menten | Vmax (in Β΅M/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1-Propanol | 1% | 0.039 | 0.024 | Β΅M/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/min), activity measured in aqueous phase |
| Activity - Michaelis-Menten | Vmax (in Β΅M/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 6% | 0.039 | 0.014 | Β΅M/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/min), activity measured in aqueous phase |
| Activity - Michaelis-Menten | Vmax (in Β΅M/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1,4-Dioxane | 6% | 0.039 | 0.006 | Β΅M/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/min), activity measured in aqueous phase |
| Activity - Michaelis-Menten | Vmax (in Β΅M/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Ethanol | 5% | 0.039 | 0.022 | Β΅M/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/min), activity measured in aqueous phase |
| Activity - Michaelis-Menten | Vmax (in Β΅M/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Tetrahydrofuran (THF) | 0.5% | 0.039 | 0.025 | Β΅M/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/min), activity measured in aqueous phase |
| Activity - Michaelis-Menten | Vmax (in Β΅M/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Pyridine | 0.5% | 0.039 | 0.013 | Β΅M/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in Β΅M/min), activity measured in aqueous phase |
| Activity - Michaelis-Menten | Vmax/Km (*1000), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Methanol | 0.5% | 3.6 | 3.6 | 1000 * 1/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in 1000 * 1/min) |
| Activity - Michaelis-Menten | Vmax/Km (*1000), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Isopropanol | 2% | 3.6 | 2.6 | 1000 * 1/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in 1000 * 1/min) |
| Activity - Michaelis-Menten | Vmax/Km (*1000), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Acetonitrile | 5% | 3.6 | 2.1 | 1000 * 1/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in 1000 * 1/min) |
| Activity - Michaelis-Menten | Vmax/Km (*1000), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1-Propanol | 1% | 3.6 | 2.9 | 1000 * 1/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in 1000 * 1/min) |
| Activity - Michaelis-Menten | Vmax/Km (*1000), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 6% | 3.6 | 1.4 | 1000 * 1/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in 1000 * 1/min) |
| Activity - Michaelis-Menten | Vmax/Km (*1000), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1,4-Dioxane | 6% | 3.6 | 0.9 | 1000 * 1/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in 1000 * 1/min) |
| Activity - Michaelis-Menten | Vmax/Km (*1000), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Ethanol | 5% | 3.6 | 2.6 | 1000 * 1/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in 1000 * 1/min) |
| Activity - Michaelis-Menten | Vmax/Km (*1000), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Tetrahydrofuran (THF) | 0.5% | 3.6 | 2.3 | 1000 * 1/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in 1000 * 1/min) |
| Activity - Michaelis-Menten | Vmax/Km (*1000), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Pyridine | 0.5% | 3.6 | 2.1 | 1000 * 1/min | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | Phenanthridine | Phenanthridinone | β | β | Classical aqueous control (in 1000 * 1/min) |
| Stability - C50 | Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Tetrahydrofuran (THF) | β | β | 1.5 | % (v/v) | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 25Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Tetrahydrofuran (THF) | β | β | 0.5 | % (v/v) | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Tetrahydrofuran (THF) | β | β | 0.5 | % (v/v) | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 45Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Dimethylformamide (DMF) | β | β | 20.0 | % (v/v) | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 25Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Dimethylformamide (DMF) | β | β | 13.0 | % (v/v) | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Dimethylformamide (DMF) | β | β | 12.0 | % (v/v) | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 45Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1-Propanol | β | β | 2.0 | % (v/v) | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 25Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1-Propanol | β | β | 2.0 | % (v/v) | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1-Propanol | β | β | 1.5 | % (v/v) | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 45Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Isopropanol | β | β | 5.0 | % (v/v) | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 25Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Isopropanol | β | β | 5.0 | % (v/v) | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Isopropanol | β | β | 4.0 | % (v/v) | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 45Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1,4-Dioxane | β | β | 10.0 | % (v/v) | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 25Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1,4-Dioxane | β | β | 9.0 | % (v/v) | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | 1,4-Dioxane | β | β | 8.0 | % (v/v) | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 45Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Pyridine | β | β | 0.5 | % (v/v) | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 25Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Pyridine | β | β | 0.5 | % (v/v) | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Pyridine | β | β | 0.5 | % (v/v) | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 45Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Methanol | β | β | 0.5 | % (v/v) | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 25Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Methanol | β | β | 0.5 | % (v/v) | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Methanol | β | β | 1.5 | % (v/v) | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 45Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Acetonitrile | β | β | 5.0 | % (v/v) | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 25Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Acetonitrile | β | β | 10.0 | % (v/v) | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Acetonitrile | β | β | 15.0 | % (v/v) | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 45Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Ethanol | β | β | 17.0 | % (v/v) | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 25Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Ethanol | β | β | 15.0 | % (v/v) | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent | Ethanol | β | β | 10.0 | % (v/v) | 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | 7.4 | 45Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Not applicable control (in % (v/v)) |
| Stability - Incubation | Activity after incubation (60 minutes at 35Β°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase | Pyridine | 0.5% | 98.0 | 0.0 | % | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | Incubation: 7.4, Assay: 4 | Incubation: 35Β°C, Assay: 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Water-incubated control (in %), activity measured in aqueous phase |
| Stability - Incubation | Activity after incubation (60 minutes at 35Β°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase | 1,4-Dioxane | 6% | 98.0 | 21.0 | % | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | Incubation: 7.4, Assay: 4 | Incubation: 35Β°C, Assay: 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Water-incubated control (in %), activity measured in aqueous phase |
| Stability - Incubation | Activity after incubation (60 minutes at 35Β°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase | Tetrahydrofuran (THF) | 0.5% | 98.0 | 43.0 | % | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | Incubation: 7.4, Assay: 4 | Incubation: 35Β°C, Assay: 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Water-incubated control (in %), activity measured in aqueous phase |
| Stability - Incubation | Activity after incubation (60 minutes at 35Β°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase | Ethanol | 5% | 98.0 | 46.0 | % | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | Incubation: 7.4, Assay: 4 | Incubation: 35Β°C, Assay: 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Water-incubated control (in %), activity measured in aqueous phase |
| Stability - Incubation | Activity after incubation (60 minutes at 35Β°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase | 1-Propanol | 1% | 98.0 | 56.0 | % | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | Incubation: 7.4, Assay: 4 | Incubation: 35Β°C, Assay: 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Water-incubated control (in %), activity measured in aqueous phase |
| Stability - Incubation | Activity after incubation (60 minutes at 35Β°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase | Isopropanol | 2% | 98.0 | 60.0 | % | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | Incubation: 7.4, Assay: 4 | Incubation: 35Β°C, Assay: 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Water-incubated control (in %), activity measured in aqueous phase |
| Stability - Incubation | Activity after incubation (60 minutes at 35Β°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase | Dimethylformamide (DMF) | 6% | 98.0 | 63.0 | % | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | Incubation: 7.4, Assay: 4 | Incubation: 35Β°C, Assay: 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Water-incubated control (in %), activity measured in aqueous phase |
| Stability - Incubation | Activity after incubation (60 minutes at 35Β°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase | Methanol | 0.5% | 98.0 | 63.0 | % | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | Incubation: 7.4, Assay: 4 | Incubation: 35Β°C, Assay: 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Water-incubated control (in %), activity measured in aqueous phase |
| Stability - Incubation | Activity after incubation (60 minutes at 35Β°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase | Acetonitrile | 5% | 98.0 | 63.0 | % | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | Incubation: 7.4, Assay: 4 | Incubation: 35Β°C, Assay: 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Water-incubated control (in %), activity measured in aqueous phase |
| Stability - Incubation | Activity after incubation (144 minutes at 35Β°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase | Pyridine | 0.5% | 100.0 | 0.0 | % | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | Incubation: 7.4, Assay: 4 | Incubation: 35Β°C, Assay: 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Water-incubated control (in %), activity measured in aqueous phase |
| Stability - Incubation | Activity after incubation (144 minutes at 35Β°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase | Ethanol | 5% | 100.0 | 7.0 | % | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | Incubation: 7.4, Assay: 4 | Incubation: 35Β°C, Assay: 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Water-incubated control (in %), activity measured in aqueous phase |
| Stability - Incubation | Activity after incubation (144 minutes at 35Β°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase | Dimethylformamide (DMF) | 6% | 100.0 | 8.0 | % | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | Incubation: 7.4, Assay: 4 | Incubation: 35Β°C, Assay: 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Water-incubated control (in %), activity measured in aqueous phase |
| Stability - Incubation | Activity after incubation (144 minutes at 35Β°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase | Isopropanol | 2% | 100.0 | 12.0 | % | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | Incubation: 7.4, Assay: 4 | Incubation: 35Β°C, Assay: 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Water-incubated control (in %), activity measured in aqueous phase |
| Stability - Incubation | Activity after incubation (144 minutes at 35Β°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase | Tetrahydrofuran (THF) | 0.5% | 100.0 | 13.0 | % | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | Incubation: 7.4, Assay: 4 | Incubation: 35Β°C, Assay: 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Water-incubated control (in %), activity measured in aqueous phase |
| Stability - Incubation | Activity after incubation (144 minutes at 35Β°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase | 1-Propanol | 1% | 100.0 | 20.0 | % | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | Incubation: 7.4, Assay: 4 | Incubation: 35Β°C, Assay: 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Water-incubated control (in %), activity measured in aqueous phase |
| Stability - Incubation | Activity after incubation (144 minutes at 35Β°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase | Methanol | 0.5% | 100.0 | 52.0 | % | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | Incubation: 7.4, Assay: 4 | Incubation: 35Β°C, Assay: 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Water-incubated control (in %), activity measured in aqueous phase |
| Stability - Incubation | Activity after incubation (144 minutes at 35Β°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase | 1,4-Dioxane | 6% | 100.0 | 52.0 | % | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | Incubation: 7.4, Assay: 4 | Incubation: 35Β°C, Assay: 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Water-incubated control (in %), activity measured in aqueous phase |
| Stability - Incubation | Activity after incubation (144 minutes at 35Β°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase | Acetonitrile | 5% | 100.0 | 54.0 | % | Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA | Incubation: 7.4, Assay: 4 | Incubation: 35Β°C, Assay: 35Β°C | 20 Β΅M Phenanthridine | Phenanthridinone | β | β | Water-incubated control (in %), activity measured in aqueous phase |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.
Visualization : Activity β Michaelis-Menten
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Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.
Visualization : Stability β C50
One bar per measurement. Colour = solvent, shade = temperature. Hover for details.
Structure
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