Aldehyde oxidase 1 from Oryctolagus cuniculus (rabbit)

Enzyme Description

Species

Extremophile

EC Number

1.2.3.1 ↗

Sequence

Length: 1334 amino acids

MEPAPELLFYVNGRKVVEKQVDPETMLLPYLRKKLRLTGTKYGCGGGGCGACTVMISRYNRVTKKIRHYPVNACLTPICSLYGAAVTTVEGIGSTTTRLHPVQERIAKFHGTQCGFCTPGMVMSMYALLRNHPEPTLDQLADALGGNLCRCTGYRPIIEAYKTFCKTSDCCQNKENGFCCLDQGINGLPEVEEENQTRPNLFSEEEYLPLDPTQELIFPPELMTMAEKQPQRTRVFSGERMMWISPVTLKALLEAKSTYPQAPVVMGNTSVGPGVKFKGIFHPVIISPDSIEELNVVSHTHSGLTLGAGLSLAQVKDILADVVQKVPEENAQTYRALLKHLGTLAGSQIRNMASLGGHIISRHLDSDLNPLLAVGNCTLNVLSKEGERQIPLDEQFLSRCPEADLKPQEILASVHIPYSRKWEFVLAFRQAQRKQNALAIVNSGMRVFFGEGDGIIRELAISYGGVGPTIICAKNSCQKLIGRSWNEEMLDTACRLILDEVSLPGSAPGGKVEFKRTLIISFLFKFYLEVSQILKRMAPGLSPHLADKYESALQDLHARYSWSTLKDQDVDARQLSQDPIGHPVMHLSGVKHATGEAIYLDDMPAVDQELFMAFVTSPRAHAKIVSTDLLEALSLPGVVDIVTAEHLQDGNTFYTEKLLAADEVLCVGQLVCAVIAESEVQAKQAAKQVKIVYEDLEPVILSIEEAIEQKSFFEPERKLEYGNVDEAFKVVDQILEGEIHMGGQEHFYMETQSVLVVPKGEDQEMDVYASTQFPKYIQDMVAAVLKLPVNKVMCHVKRVGGAFGGKVFKASIMAAIAAFAANKHGRAVRCILERGEDMLITGGRHPYLGKYKAGFMNDGRIVALDVEHYSNGGCSLDESLLVIEMGLLKMENAYKFPNLRCRGWACRTNLPSNTAFRGFGFPQAGLITECCITEVAAKCGLSPEKVRAINFYKEIDQTPYKQEINAKNLTQCWNECLAKSSYFQRKVAVEKFNAENYWKQRGLAIIPFKYPRGLGSVAYGQAAALVHVYLDGSVLVTHGGIEMGQGVHTKMIQVVSRELKMPMSNVHLRGTSTETVPNTNASGGSVVADLNGLAVKDACQTLLKRLEPIINKNPQGTWKEWAQAAFDKSISLSATGYFRGYDSNIDWDKGEGHPFEYFVYGAACSEVEIDCLTGDHKTIRTDIVMDVGYSINPALDIGQVEGAFIQGMGLYTIEELHYSPQGILYSRGPNQYKIPAICDIPAELNVTFLPPSEKSNTLYSSKGLGESGVFMGCSVFFAIREAVCAARQARGLSAPWKLSSPLTPEKIRMACEDKFTKMIPRDKPGSYVPWNVPV

Mohammad-Reza Rashidi et al. (2013) — Aldehyde Oxidase Activity and Stability in Water-Miscible Organic Solvents

Applied Biochemistry and Biotechnology · doi:10.1007/s12010-012-0036-7 ↗ · Activity - Classical Activity - Michaelis-Menten Stability - C50 Stability - Incubation

243 measurements ▼

Database ID

UniProt: P80456 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Purified, eukaryote Oryctolagus cuniculus (rabbit)

Experimental Data (243 measurements)

243 measurements — page 12 of 13

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - C50	Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Acetonitrile	—	—	10.0	% (v/v)	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Acetonitrile	—	—	15.0	% (v/v)	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	45°C	20 µM Phenanthridine	Phenanthridinone	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Ethanol	—	—	17.0	% (v/v)	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	25°C	20 µM Phenanthridine	Phenanthridinone	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Ethanol	—	—	15.0	% (v/v)	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Ethanol	—	—	10.0	% (v/v)	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	45°C	20 µM Phenanthridine	Phenanthridinone	—	—	Not applicable control (in % (v/v))
Stability - Incubation	Activity after incubation (60 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	Pyridine	0.5%	98.0	0.0	%	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	1,4-Dioxane	6%	98.0	21.0	%	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	Tetrahydrofuran (THF)	0.5%	98.0	43.0	%	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	Ethanol	5%	98.0	46.0	%	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	1-Propanol	1%	98.0	56.0	%	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	Isopropanol	2%	98.0	60.0	%	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	Dimethylformamide (DMF)	6%	98.0	63.0	%	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	Methanol	0.5%	98.0	63.0	%	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	Acetonitrile	5%	98.0	63.0	%	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (144 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	Pyridine	0.5%	100.0	0.0	%	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (144 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	Ethanol	5%	100.0	7.0	%	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (144 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	Dimethylformamide (DMF)	6%	100.0	8.0	%	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (144 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	Isopropanol	2%	100.0	12.0	%	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (144 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	Tetrahydrofuran (THF)	0.5%	100.0	13.0	%	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (144 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	1-Propanol	1%	100.0	20.0	%	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase

Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	0.5%	100.0	56.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	1%	100.0	42.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	1.5%	100.0	35.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	2%	100.0	30.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	2.5%	100.0	23.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	5%	100.0	10.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	10%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	15%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	20%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	25%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	30%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	35%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Dimethylformamide (DMF)	0.5%	100.0	98.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Dimethylformamide (DMF)	1%	100.0	94.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Dimethylformamide (DMF)	1.5%	100.0	90.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Dimethylformamide (DMF)	2%	100.0	87.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Dimethylformamide (DMF)	2.5%	100.0	82.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Dimethylformamide (DMF)	5%	100.0	73.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Dimethylformamide (DMF)	10%	100.0	63.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Dimethylformamide (DMF)	15%	100.0	48.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Dimethylformamide (DMF)	20%	100.0	39.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Dimethylformamide (DMF)	25%	100.0	25.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Dimethylformamide (DMF)	30%	100.0	10.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Dimethylformamide (DMF)	35%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1-Propanol	0.5%	100.0	69.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1-Propanol	1%	100.0	60.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1-Propanol	1.5%	100.0	54.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1-Propanol	2%	100.0	49.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1-Propanol	2.5%	100.0	40.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1-Propanol	5%	100.0	30.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1-Propanol	10%	100.0	17.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1-Propanol	15%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1-Propanol	20%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1-Propanol	25%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1-Propanol	30%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1-Propanol	35%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Isopropanol	0.5%	100.0	80.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Isopropanol	1%	100.0	74.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Isopropanol	1.5%	100.0	70.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Isopropanol	2%	100.0	65.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Isopropanol	2.5%	100.0	56.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Isopropanol	5%	100.0	43.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Isopropanol	10%	100.0	26.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Isopropanol	15%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Isopropanol	20%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Isopropanol	25%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Isopropanol	30%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Isopropanol	35%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1,4-Dioxane	0.5%	100.0	95.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1,4-Dioxane	1%	100.0	90.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1,4-Dioxane	1.5%	100.0	85.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1,4-Dioxane	2%	100.0	78.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1,4-Dioxane	2.5%	100.0	70.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1,4-Dioxane	5%	100.0	60.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1,4-Dioxane	10%	100.0	43.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1,4-Dioxane	15%	100.0	28.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1,4-Dioxane	20%	100.0	13.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1,4-Dioxane	25%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1,4-Dioxane	30%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1,4-Dioxane	35%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Pyridine	0.5%	100.0	40.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Pyridine	1%	100.0	22.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Pyridine	1.5%	100.0	17.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Pyridine	2%	100.0	13.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Pyridine	2.5%	100.0	5.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Pyridine	5%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Pyridine	10%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Pyridine	15%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Pyridine	20%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Pyridine	25%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Pyridine	30%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Pyridine	35%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Methanol	0.5%	100.0	65.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Methanol	1%	100.0	45.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Methanol	1.5%	100.0	38.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Methanol	2%	100.0	32.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Methanol	2.5%	100.0	26.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Methanol	5%	100.0	15.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Methanol	10%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Methanol	15%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Methanol	20%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Methanol	25%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Methanol	30%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Methanol	35%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Acetonitrile	0.5%	100.0	95.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Acetonitrile	1%	100.0	88.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Acetonitrile	1.5%	100.0	83.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Acetonitrile	2%	100.0	79.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Acetonitrile	2.5%	100.0	72.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Acetonitrile	5%	100.0	63.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Acetonitrile	10%	100.0	41.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Acetonitrile	15%	100.0	17.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Acetonitrile	20%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Acetonitrile	25%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Acetonitrile	30%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Acetonitrile	35%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Ethanol	0.5%	100.0	98.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Ethanol	1%	100.0	93.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Ethanol	1.5%	100.0	88.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Ethanol	2%	100.0	83.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Ethanol	2.5%	100.0	77.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Ethanol	5%	100.0	68.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Ethanol	10%	100.0	55.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Ethanol	15%	100.0	42.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Ethanol	20%	100.0	33.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Ethanol	25%	100.0	20.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Ethanol	30%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Ethanol	35%	100.0	0.0	%	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	0.5%	57.0	28.0	µM/mg product/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	25°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	0.5%	71.0	49.0	µM/mg product/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	0.5%	94.0	65.0	µM/mg product/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	45°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Dimethylformamide (DMF)	6%	57.0	37.0	µM/mg product/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	25°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Dimethylformamide (DMF)	6%	71.0	50.0	µM/mg product/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Dimethylformamide (DMF)	6%	94.0	73.0	µM/mg product/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	45°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1-Propanol	1%	57.0	21.0	µM/mg product/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	25°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1-Propanol	1%	71.0	37.0	µM/mg product/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1-Propanol	1%	94.0	57.0	µM/mg product/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	45°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Isopropanol	2%	57.0	22.0	µM/mg product/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	25°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Isopropanol	2%	71.0	34.0	µM/mg product/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Isopropanol	2%	94.0	73.0	µM/mg product/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	45°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1,4-Dioxane	6%	57.0	20.0	µM/mg product/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	25°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1,4-Dioxane	6%	71.0	28.0	µM/mg product/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1,4-Dioxane	6%	94.0	33.0	µM/mg product/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	45°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Pyridine	0.5%	57.0	13.0	µM/mg product/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	25°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Pyridine	0.5%	71.0	25.0	µM/mg product/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Pyridine	0.5%	94.0	33.0	µM/mg product/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	45°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Methanol	0.5%	57.0	35.0	µM/mg product/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	25°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Methanol	0.5%	71.0	50.0	µM/mg product/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Methanol	0.5%	94.0	82.0	µM/mg product/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	45°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Acetonitrile	5%	57.0	21.0	µM/mg product/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	25°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Acetonitrile	5%	71.0	48.0	µM/mg product/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Acetonitrile	5%	94.0	67.0	µM/mg product/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	45°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Ethanol	5%	57.0	30.0	µM/mg product/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	25°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Ethanol	5%	71.0	45.0	µM/mg product/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Ethanol	5%	94.0	67.0	µM/mg product/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	45°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)	Tetrahydrofuran (THF)	0.5%	17.0	10.0	µM/mg product/min	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	6	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)	Tetrahydrofuran (THF)	0.5%	50.0	30.0	µM/mg product/min	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)	Tetrahydrofuran (THF)	0.5%	51.0	34.0	µM/mg product/min	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	8	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)	Tetrahydrofuran (THF)	0.5%	63.0	36.0	µM/mg product/min	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	9	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)	Dimethylformamide (DMF)	6%	17.0	10.0	µM/mg product/min	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	6	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)	Dimethylformamide (DMF)	6%	50.0	21.0	µM/mg product/min	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)	Dimethylformamide (DMF)	6%	51.0	39.0	µM/mg product/min	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	8	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)	Dimethylformamide (DMF)	6%	63.0	43.0	µM/mg product/min	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	9	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)	1-Propanol	1%	17.0	11.0	µM/mg product/min	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	6	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)	1-Propanol	1%	50.0	28.0	µM/mg product/min	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)	1-Propanol	1%	51.0	36.0	µM/mg product/min	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	8	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)	1-Propanol	1%	63.0	22.0	µM/mg product/min	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	9	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)	Isopropanol	2%	17.0	7.0	µM/mg product/min	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	6	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)	Isopropanol	2%	50.0	21.0	µM/mg product/min	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)	Isopropanol	2%	51.0	28.0	µM/mg product/min	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	8	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)	Isopropanol	2%	63.0	35.0	µM/mg product/min	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	9	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)	1,4-Dioxane	6%	17.0	10.0	µM/mg product/min	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	6	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)	1,4-Dioxane	6%	50.0	28.0	µM/mg product/min	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)	1,4-Dioxane	6%	51.0	30.0	µM/mg product/min	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	8	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)	1,4-Dioxane	6%	63.0	28.0	µM/mg product/min	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	9	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)	Pyridine	0.5%	17.0	8.0	µM/mg product/min	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	6	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)	Pyridine	0.5%	50.0	18.0	µM/mg product/min	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)	Pyridine	0.5%	51.0	27.0	µM/mg product/min	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	8	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)	Pyridine	0.5%	63.0	27.0	µM/mg product/min	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	9	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)	Methanol	0.5%	17.0	11.0	µM/mg product/min	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	6	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)	Methanol	0.5%	50.0	29.0	µM/mg product/min	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)	Methanol	0.5%	51.0	42.0	µM/mg product/min	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	8	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)	Methanol	0.5%	63.0	35.0	µM/mg product/min	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	9	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)	Acetonitrile	5%	17.0	3.0	µM/mg product/min	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	6	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)	Acetonitrile	5%	50.0	32.0	µM/mg product/min	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)	Acetonitrile	5%	51.0	29.0	µM/mg product/min	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	8	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)	Acetonitrile	5%	63.0	27.0	µM/mg product/min	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	9	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)	Ethanol	5%	17.0	11.0	µM/mg product/min	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	6	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)	Ethanol	5%	50.0	26.0	µM/mg product/min	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)	Ethanol	5%	51.0	34.0	µM/mg product/min	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	8	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Classical	Activity (in µM/mg protein/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement)	Ethanol	5%	63.0	28.0	µM/mg product/min	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	9	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/mg product/min)
Activity - Michaelis-Menten	Km (in µM), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Methanol	0.5%	11.0	5.0	µM	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM), activity measured in aqueous phase
Activity - Michaelis-Menten	Km (in µM), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Isopropanol	2%	11.0	7.69	µM	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM), activity measured in aqueous phase
Activity - Michaelis-Menten	Km (in µM), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Acetonitrile	5%	11.0	6.3	µM	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM), activity measured in aqueous phase
Activity - Michaelis-Menten	Km (in µM), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1-Propanol	1%	11.0	8.33	µM	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM), activity measured in aqueous phase
Activity - Michaelis-Menten	Km (in µM), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Dimethylformamide (DMF)	6%	11.0	10.0	µM	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM), activity measured in aqueous phase
Activity - Michaelis-Menten	Km (in µM), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1,4-Dioxane	6%	11.0	6.66	µM	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM), activity measured in aqueous phase
Activity - Michaelis-Menten	Km (in µM), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Ethanol	5%	11.0	8.33	µM	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM), activity measured in aqueous phase
Activity - Michaelis-Menten	Km (in µM), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	0.5%	11.0	11.0	µM	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM), activity measured in aqueous phase
Activity - Michaelis-Menten	Km (in µM), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Pyridine	0.5%	11.0	6.25	µM	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM), activity measured in aqueous phase
Activity - Michaelis-Menten	Vmax (in µM/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Methanol	0.5%	0.039	0.018	µM/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/min), activity measured in aqueous phase
Activity - Michaelis-Menten	Vmax (in µM/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Isopropanol	2%	0.039	0.02	µM/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/min), activity measured in aqueous phase
Activity - Michaelis-Menten	Vmax (in µM/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Acetonitrile	5%	0.039	0.013	µM/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/min), activity measured in aqueous phase
Activity - Michaelis-Menten	Vmax (in µM/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1-Propanol	1%	0.039	0.024	µM/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/min), activity measured in aqueous phase
Activity - Michaelis-Menten	Vmax (in µM/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Dimethylformamide (DMF)	6%	0.039	0.014	µM/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/min), activity measured in aqueous phase
Activity - Michaelis-Menten	Vmax (in µM/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1,4-Dioxane	6%	0.039	0.006	µM/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/min), activity measured in aqueous phase
Activity - Michaelis-Menten	Vmax (in µM/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Ethanol	5%	0.039	0.022	µM/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/min), activity measured in aqueous phase
Activity - Michaelis-Menten	Vmax (in µM/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	0.5%	0.039	0.025	µM/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/min), activity measured in aqueous phase
Activity - Michaelis-Menten	Vmax (in µM/min), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Pyridine	0.5%	0.039	0.013	µM/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in µM/min), activity measured in aqueous phase
Activity - Michaelis-Menten	Vmax/Km (*1000), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Methanol	0.5%	3.6	3.6	1000 * 1/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in 1000 * 1/min)
Activity - Michaelis-Menten	Vmax/Km (*1000), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Isopropanol	2%	3.6	2.6	1000 * 1/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in 1000 * 1/min)
Activity - Michaelis-Menten	Vmax/Km (*1000), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Acetonitrile	5%	3.6	2.1	1000 * 1/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in 1000 * 1/min)
Activity - Michaelis-Menten	Vmax/Km (*1000), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1-Propanol	1%	3.6	2.9	1000 * 1/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in 1000 * 1/min)
Activity - Michaelis-Menten	Vmax/Km (*1000), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Dimethylformamide (DMF)	6%	3.6	1.4	1000 * 1/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in 1000 * 1/min)
Activity - Michaelis-Menten	Vmax/Km (*1000), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1,4-Dioxane	6%	3.6	0.9	1000 * 1/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in 1000 * 1/min)
Activity - Michaelis-Menten	Vmax/Km (*1000), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Ethanol	5%	3.6	2.6	1000 * 1/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in 1000 * 1/min)
Activity - Michaelis-Menten	Vmax/Km (*1000), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	0.5%	3.6	2.3	1000 * 1/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in 1000 * 1/min)
Activity - Michaelis-Menten	Vmax/Km (*1000), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Pyridine	0.5%	3.6	2.1	1000 * 1/min	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	Phenanthridine	Phenanthridinone	—	—	Classical aqueous control (in 1000 * 1/min)
Stability - C50	Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	—	—	1.5	% (v/v)	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	25°C	20 µM Phenanthridine	Phenanthridinone	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	—	—	0.5	% (v/v)	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	—	—	0.5	% (v/v)	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	45°C	20 µM Phenanthridine	Phenanthridinone	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Dimethylformamide (DMF)	—	—	20.0	% (v/v)	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	25°C	20 µM Phenanthridine	Phenanthridinone	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Dimethylformamide (DMF)	—	—	13.0	% (v/v)	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Dimethylformamide (DMF)	—	—	12.0	% (v/v)	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	45°C	20 µM Phenanthridine	Phenanthridinone	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1-Propanol	—	—	2.0	% (v/v)	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	25°C	20 µM Phenanthridine	Phenanthridinone	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1-Propanol	—	—	2.0	% (v/v)	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1-Propanol	—	—	1.5	% (v/v)	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	45°C	20 µM Phenanthridine	Phenanthridinone	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Isopropanol	—	—	5.0	% (v/v)	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	25°C	20 µM Phenanthridine	Phenanthridinone	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Isopropanol	—	—	5.0	% (v/v)	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Isopropanol	—	—	4.0	% (v/v)	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	45°C	20 µM Phenanthridine	Phenanthridinone	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1,4-Dioxane	—	—	10.0	% (v/v)	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	25°C	20 µM Phenanthridine	Phenanthridinone	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1,4-Dioxane	—	—	9.0	% (v/v)	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	1,4-Dioxane	—	—	8.0	% (v/v)	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	45°C	20 µM Phenanthridine	Phenanthridinone	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Pyridine	—	—	0.5	% (v/v)	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	25°C	20 µM Phenanthridine	Phenanthridinone	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Pyridine	—	—	0.5	% (v/v)	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Pyridine	—	—	0.5	% (v/v)	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	45°C	20 µM Phenanthridine	Phenanthridinone	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Methanol	—	—	0.5	% (v/v)	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	25°C	20 µM Phenanthridine	Phenanthridinone	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Methanol	—	—	0.5	% (v/v)	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Methanol	—	—	1.5	% (v/v)	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	45°C	20 µM Phenanthridine	Phenanthridinone	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Acetonitrile	—	—	5.0	% (v/v)	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	25°C	20 µM Phenanthridine	Phenanthridinone	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Acetonitrile	—	—	10.0	% (v/v)	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Acetonitrile	—	—	15.0	% (v/v)	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	45°C	20 µM Phenanthridine	Phenanthridinone	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Ethanol	—	—	17.0	% (v/v)	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	25°C	20 µM Phenanthridine	Phenanthridinone	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Ethanol	—	—	15.0	% (v/v)	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent volume for which half of the activity is lost, as measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in the presence of organic solvent	Ethanol	—	—	10.0	% (v/v)	67 mM Sorenson phosphate buffer, 0.1 MM EDTA	7.4	45°C	20 µM Phenanthridine	Phenanthridinone	—	—	Not applicable control (in % (v/v))
Stability - Incubation	Activity after incubation (60 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	Pyridine	0.5%	98.0	0.0	%	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	1,4-Dioxane	6%	98.0	21.0	%	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	Tetrahydrofuran (THF)	0.5%	98.0	43.0	%	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	Ethanol	5%	98.0	46.0	%	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	1-Propanol	1%	98.0	56.0	%	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	Isopropanol	2%	98.0	60.0	%	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	Dimethylformamide (DMF)	6%	98.0	63.0	%	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	Methanol	0.5%	98.0	63.0	%	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	Acetonitrile	5%	98.0	63.0	%	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (144 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	Pyridine	0.5%	100.0	0.0	%	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (144 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	Ethanol	5%	100.0	7.0	%	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (144 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	Dimethylformamide (DMF)	6%	100.0	8.0	%	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (144 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	Isopropanol	2%	100.0	12.0	%	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (144 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	Tetrahydrofuran (THF)	0.5%	100.0	13.0	%	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (144 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	1-Propanol	1%	100.0	20.0	%	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (144 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	Methanol	0.5%	100.0	52.0	%	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (144 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	1,4-Dioxane	6%	100.0	52.0	%	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase
Stability - Incubation	Activity after incubation (144 minutes at 35°C), measured by absorbance spectrophotometry (phenathridinone absorbance measurement, 322 nm) in aqueous phase	Acetonitrile	5%	100.0	54.0	%	Incubation: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA, Assay: 67 mM Sorenson phosphate buffer, 0.1 MM EDTA	Incubation: 7.4, Assay: 4	Incubation: 35°C, Assay: 35°C	20 µM Phenanthridine	Phenanthridinone	—	—	Water-incubated control (in %), activity measured in aqueous phase

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Activity — Michaelis-Menten

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — C₅₀

One bar per measurement. Colour = solvent, shade = temperature. Hover for details.

Structure

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Aldehyde oxidase 1 from Oryctolagus cuniculus (rabbit)

Enzyme Description

Sequence

Experimental Data (243 measurements)

Visualization : Activity — Classical

Visualization : Activity — Michaelis-Menten

Visualization : Stability — Incubation

Visualization : Stability — C50

Structure

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Visualization : Stability — C₅₀