Ξ±-chymotrypsin from Bos taurus (bovine)
Enzyme Description
Sequence
CGVPAIQPVLSGLSRIVNGEEAVPGSWPWQVSLQDKTGFHFCGGSLINENWVVTAAHCGVTTSDVVVAGEFDQGSSSEKIQKLKIAKVFKNSKYNSLTINNDITLLKLSTAASFSQTVSAVCLPSASDDFAAGTTCVTTGWGLTRYTNANTPDRLQQASLPLLSNTNCKKYWGTKIKDAMICAGASGVSSCMGDSGGPLVCKKNGAWTLVGIVSWGSSTCSTSTPGVYARVTALVNWVQQTLAAN
Vadim V. Mozhaev et al. (1989)
β Catalytic activity and denaturation of enzymes in water/organic cosolvent mixtures
European Journal of Biochemistry
Β· doi:10.1111/j.1432-1033.1989.tb15055.x β
Β· Stability - C50
▼
Database ID
UniProt: P00766 β
Sequence Annotation
Inferred - from protein name
Protein Source
Commercially purchased
Experimental Data (16 measurements)
16 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - C50 | Volume of organic solvent (in %) at which 50% of initial activity is lost, as measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 375 nm) in the presence of organic solvent | Methanol | β | β | 32 | % (v/v) | 50 mM phosphate buffer | 6.5-7 | 25 Β°C | 50-400 micoMolar BTPNA (Benzoyl-L-tyrosine p-nitroanilide) | N-Benzoyl-L-tyrosine , p-Nitroaniline | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume of organic solvent (in %) at which 50% of initial activity is lost, as measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 375 nm) in the presence of organic solvent | Ethanol | β | β | 36 | % (v/v) | 50 mM phosphate buffer | 6.5-7 | 25 Β°C | 50-400 micoMolar BTPNA (Benzoyl-L-tyrosine p-nitroanilide) | N-Benzoyl-L-tyrosine , p-Nitroaniline | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume of organic solvent (in %) at which 50% of initial activity is lost, as measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 375 nm) in the presence of organic solvent | 1-Propanol | β | β | 27 | % (v/v) | 50 mM phosphate buffer | 6.5-7 | 25 Β°C | 50-400 micoMolar BTPNA (Benzoyl-L-tyrosine p-nitroanilide) | N-Benzoyl-L-tyrosine , p-Nitroaniline | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume of organic solvent (in %) at which 50% of initial activity is lost, as measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 375 nm) in the presence of organic solvent | Isopropanol | β | β | 33 | % (v/v) | 50 mM phosphate buffer | 6.5-7 | 25 Β°C | 50-400 micoMolar BTPNA (Benzoyl-L-tyrosine p-nitroanilide) | N-Benzoyl-L-tyrosine , p-Nitroaniline | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume of organic solvent (in %) at which 50% of initial activity is lost, as measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 375 nm) in the presence of organic solvent | Isobutanol | β | β | 10 | % (v/v) | 50 mM phosphate buffer | 6.5-7 | 25 Β°C | 50-400 micoMolar BTPNA (Benzoyl-L-tyrosine p-nitroanilide) | N-Benzoyl-L-tyrosine , p-Nitroaniline | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume of organic solvent (in %) at which 50% of initial activity is lost, as measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 375 nm) in the presence of organic solvent | Ethylene Glycol | β | β | 72 | % (v/v) | 50 mM phosphate buffer | 6.5-7 | 25 Β°C | 50-400 micoMolar BTPNA (Benzoyl-L-tyrosine p-nitroanilide) | N-Benzoyl-L-tyrosine , p-Nitroaniline | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume of organic solvent (in %) at which 50% of initial activity is lost, as measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 375 nm) in the presence of organic solvent | Propylene Glycol | β | β | 60 | % (v/v) | 50 mM phosphate buffer | 6.5-7 | 25 Β°C | 50-400 micoMolar BTPNA (Benzoyl-L-tyrosine p-nitroanilide) | N-Benzoyl-L-tyrosine , p-Nitroaniline | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume of organic solvent (in %) at which 50% of initial activity is lost, as measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 375 nm) in the presence of organic solvent | 1,4-Butanediol | β | β | 65 | % (v/v) | 50 mM phosphate buffer | 6.5-7 | 25 Β°C | 50-400 micoMolar BTPNA (Benzoyl-L-tyrosine p-nitroanilide) | N-Benzoyl-L-tyrosine , p-Nitroaniline | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume of organic solvent (in %) at which 50% of initial activity is lost, as measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 375 nm) in the presence of organic solvent | 1,3-Butanediol | β | β | 56 | % (v/v) | 50 mM phosphate buffer | 6.5-7 | 25 Β°C | 50-400 micoMolar BTPNA (Benzoyl-L-tyrosine p-nitroanilide) | N-Benzoyl-L-tyrosine , p-Nitroaniline | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume of organic solvent (in %) at which 50% of initial activity is lost, as measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 375 nm) in the presence of organic solvent | 2,3-Butanediol | β | β | 39 | % (v/v) | 50 mM phosphate buffer | 6.5-7 | 25 Β°C | 50-400 micoMolar BTPNA (Benzoyl-L-tyrosine p-nitroanilide) | N-Benzoyl-L-tyrosine , p-Nitroaniline | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume of organic solvent (in %) at which 50% of initial activity is lost, as measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 375 nm) in the presence of organic solvent | Hexylene Glycol | β | β | 50 | % (v/v) | 50 mM phosphate buffer | 6.5-7 | 25 Β°C | 50-400 micoMolar BTPNA (Benzoyl-L-tyrosine p-nitroanilide) | N-Benzoyl-L-tyrosine , p-Nitroaniline | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume of organic solvent (in %) at which 50% of initial activity is lost, as measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 375 nm) in the presence of organic solvent | Glycerol | β | β | 73 | % (v/v) | 50 mM phosphate buffer | 6.5-7 | 25 Β°C | 50-400 micoMolar BTPNA (Benzoyl-L-tyrosine p-nitroanilide) | N-Benzoyl-L-tyrosine , p-Nitroaniline | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume of organic solvent (in %) at which 50% of initial activity is lost, as measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 375 nm) in the presence of organic solvent | 1,2,6-Hexanetriol | β | β | 68 | % (v/v) | 50 mM phosphate buffer | 6.5-7 | 25 Β°C | 50-400 micoMolar BTPNA (Benzoyl-L-tyrosine p-nitroanilide) | N-Benzoyl-L-tyrosine , p-Nitroaniline | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume of organic solvent (in %) at which 50% of initial activity is lost, as measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 375 nm) in the presence of organic solvent | Formamide | β | β | 23 | % (v/v) | 50 mM phosphate buffer | 6.5-7 | 25 Β°C | 50-400 micoMolar BTPNA (Benzoyl-L-tyrosine p-nitroanilide) | N-Benzoyl-L-tyrosine , p-Nitroaniline | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume of organic solvent (in %) at which 50% of initial activity is lost, as measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 375 nm) in the presence of organic solvent | n-Methylformamide (NMF) | β | β | 29 | % (v/v) | 50 mM phosphate buffer | 6.5-7 | 25 Β°C | 50-400 micoMolar BTPNA (Benzoyl-L-tyrosine p-nitroanilide) | N-Benzoyl-L-tyrosine , p-Nitroaniline | β | β | Not applicable control (in % (v/v)) |
| Stability - C50 | Volume of organic solvent (in %) at which 50% of initial activity is lost, as measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 375 nm) in the presence of organic solvent | Dimethylformamide (DMF) | β | β | 26 | % (v/v) | 50 mM phosphate buffer | 6.5-7 | 25 Β°C | 50-400 micoMolar BTPNA (Benzoyl-L-tyrosine p-nitroanilide) | N-Benzoyl-L-tyrosine , p-Nitroaniline | β | β | Not applicable control (in % (v/v)) |
Visualization : Stability β C50
Vadim V. Mozhaev et al. (1989)
One bar per measurement. Colour = solvent, shade = temperature. Hover for details.
Rosa M. Blanco et al. (1992)
β Effect of immiscible organic solvents on activity/stability of native chymotrypsin and immobilized-stabilized derivatives
Biotechnology and Bioengineering
Β· doi:10.1002/bit.260390112 β
Β· Stability - Incubation
▼
Database ID
UniProt: P00766 β
Sequence Annotation
Inferred - from protein name
Protein Source
Commercially purchased
Experimental Data (27 measurements)
27 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (20 days at 25Β°C), measured by absorbance spectrophotometry (benzoyl-L-tyrosine absorbance measurement, 258 nm) in aqueous phase | Dichloromethane | 1.5% | 100 | 20 | % | 0.1 M phosphate buffer | 7 | 25Β°C | 0.4 mM N-Benzoyl-L-tyrosine ethyl ester | Ethanol , N-Benzoyl-L-tyrosine | β | No, "unstirred" incubation | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("in the absence of organic solvents except the very small quantity taken with the 50-microL aliquot")/Deducted organic solvent volume (annotated as "saturating concentration") |
| Stability - Incubation | Activity after incubation (40 days at 25Β°C), measured by absorbance spectrophotometry (benzoyl-L-tyrosine absorbance measurement, 258 nm) in aqueous phase | Chloroform | 0.5% | 100 | 0 | % | 0.1 M phosphate buffer | 7 | 25Β°C | 0.4 mM N-Benzoyl-L-tyrosine ethyl ester | Ethanol , N-Benzoyl-L-tyrosine | β | No, "unstirred" incubation | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("in the absence of organic solvents except the very small quantity taken with the 50-microL aliquot")/Deducted organic solvent volume (annotated as "saturating concentration") |
| Stability - Incubation | Activity after incubation (30 days at 25Β°C), measured by absorbance spectrophotometry (benzoyl-L-tyrosine absorbance measurement, 258 nm) in aqueous phase | Chloroform | 0.5% | 100 | 0 | % | 0.1 M phosphate buffer | 7 | 25Β°C | 0.4 mM N-Benzoyl-L-tyrosine ethyl ester | Ethanol , N-Benzoyl-L-tyrosine | β | No, "unstirred" incubation | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("in the absence of organic solvents except the very small quantity taken with the 50-microL aliquot")/Deducted organic solvent volume (annotated as "saturating concentration") |
| Stability - Incubation | Activity after incubation (20 days at 25Β°C), measured by absorbance spectrophotometry (benzoyl-L-tyrosine absorbance measurement, 258 nm) in aqueous phase | Chloroform | 0.5% | 100 | 5 | % | 0.1 M phosphate buffer | 7 | 25Β°C | 0.4 mM N-Benzoyl-L-tyrosine ethyl ester | Ethanol , N-Benzoyl-L-tyrosine | β | No, "unstirred" incubation | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("in the absence of organic solvents except the very small quantity taken with the 50-microL aliquot")/Deducted organic solvent volume (annotated as "saturating concentration") |
| Stability - Incubation | Activity after incubation (15 days at 25Β°C), measured by absorbance spectrophotometry (benzoyl-L-tyrosine absorbance measurement, 258 nm) in aqueous phase | Chloroform | 0.5% | 100 | 15 | % | 0.1 M phosphate buffer | 7 | 25Β°C | 0.4 mM N-Benzoyl-L-tyrosine ethyl ester | Ethanol , N-Benzoyl-L-tyrosine | β | No, "unstirred" incubation | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("in the absence of organic solvents except the very small quantity taken with the 50-microL aliquot")/Deducted organic solvent volume (annotated as "saturating concentration") |
| Stability - Incubation | Activity after incubation (10 days at 25Β°C), measured by absorbance spectrophotometry (benzoyl-L-tyrosine absorbance measurement, 258 nm) in aqueous phase | Chloroform | 0.5% | 100 | 31 | % | 0.1 M phosphate buffer | 7 | 25Β°C | 0.4 mM N-Benzoyl-L-tyrosine ethyl ester | Ethanol , N-Benzoyl-L-tyrosine | β | No, "unstirred" incubation | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("in the absence of organic solvents except the very small quantity taken with the 50-microL aliquot")/Deducted organic solvent volume (annotated as "saturating concentration") |
| Stability - Incubation | Activity after incubation (7 days at 25Β°C), measured by absorbance spectrophotometry (benzoyl-L-tyrosine absorbance measurement, 258 nm) in aqueous phase | Chloroform | 0.5% | 100 | 50 | % | 0.1 M phosphate buffer | 7 | 25Β°C | 0.4 mM N-Benzoyl-L-tyrosine ethyl ester | Ethanol , N-Benzoyl-L-tyrosine | β | No, "unstirred" incubation | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("in the absence of organic solvents except the very small quantity taken with the 50-microL aliquot")/Deducted organic solvent volume (annotated as "saturating concentration") |
| Stability - Incubation | Activity after incubation (5 days at 25Β°C), measured by absorbance spectrophotometry (benzoyl-L-tyrosine absorbance measurement, 258 nm) in aqueous phase | Chloroform | 0.5% | 100 | 69 | % | 0.1 M phosphate buffer | 7 | 25Β°C | 0.4 mM N-Benzoyl-L-tyrosine ethyl ester | Ethanol , N-Benzoyl-L-tyrosine | β | No, "unstirred" incubation | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("in the absence of organic solvents except the very small quantity taken with the 50-microL aliquot")/Deducted organic solvent volume (annotated as "saturating concentration") |
| Stability - Incubation | Activity after incubation (4 days at 25Β°C), measured by absorbance spectrophotometry (benzoyl-L-tyrosine absorbance measurement, 258 nm) in aqueous phase | Chloroform | 0.5% | 100 | 79 | % | 0.1 M phosphate buffer | 7 | 25Β°C | 0.4 mM N-Benzoyl-L-tyrosine ethyl ester | Ethanol , N-Benzoyl-L-tyrosine | β | No, "unstirred" incubation | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("in the absence of organic solvents except the very small quantity taken with the 50-microL aliquot")/Deducted organic solvent volume (annotated as "saturating concentration") |
| Stability - Incubation | Activity after incubation (3 days at 25Β°C), measured by absorbance spectrophotometry (benzoyl-L-tyrosine absorbance measurement, 258 nm) in aqueous phase | Chloroform | 0.5% | 100 | 86 | % | 0.1 M phosphate buffer | 7 | 25Β°C | 0.4 mM N-Benzoyl-L-tyrosine ethyl ester | Ethanol , N-Benzoyl-L-tyrosine | β | No, "unstirred" incubation | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("in the absence of organic solvents except the very small quantity taken with the 50-microL aliquot")/Deducted organic solvent volume (annotated as "saturating concentration") |
| Stability - Incubation | Activity after incubation (2 days at 25Β°C), measured by absorbance spectrophotometry (benzoyl-L-tyrosine absorbance measurement, 258 nm) in aqueous phase | Chloroform | 0.5% | 100 | 93 | % | 0.1 M phosphate buffer | 7 | 25Β°C | 0.4 mM N-Benzoyl-L-tyrosine ethyl ester | Ethanol , N-Benzoyl-L-tyrosine | β | No, "unstirred" incubation | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("in the absence of organic solvents except the very small quantity taken with the 50-microL aliquot")/Deducted organic solvent volume (annotated as "saturating concentration") |
| Stability - Incubation | Activity after incubation (40 days at 25Β°C ), measured by absorbance spectrophotometry (benzoyl-L-tyrosine absorbance measurement, 258 nm) in aqueous phase | Dichloromethane | 1.5% | 100 | 10 | % | 0.1 M phosphate buffer | 7 | 25Β°C | 0.4 mM N-Benzoyl-L-tyrosine ethyl ester | Ethanol , N-Benzoyl-L-tyrosine | β | No, "unstirred" incubation | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("in the absence of organic solvents except the very small quantity taken with the 50-microL aliquot")/Deducted organic solvent volume (annotated as "saturating concentration") |
| Stability - Incubation | Activity after incubation (30 days at 25Β°C), measured by absorbance spectrophotometry (benzoyl-L-tyrosine absorbance measurement, 258 nm) in aqueous phase | Dichloromethane | 1.5% | 100 | 17 | % | 0.1 M phosphate buffer | 7 | 25Β°C | 0.4 mM N-Benzoyl-L-tyrosine ethyl ester | Ethanol , N-Benzoyl-L-tyrosine | β | No, "unstirred" incubation | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("in the absence of organic solvents except the very small quantity taken with the 50-microL aliquot")/Deducted organic solvent volume (annotated as "saturating concentration") |
| Stability - Incubation | Activity after incubation (25 days at 25Β°C), measured by absorbance spectrophotometry (benzoyl-L-tyrosine absorbance measurement, 258 nm) in aqueous phase | Dichloromethane | 1.5% | 100 | 19 | % | 0.1 M phosphate buffer | 7 | 25Β°C | 0.4 mM N-Benzoyl-L-tyrosine ethyl ester | Ethanol , N-Benzoyl-L-tyrosine | β | No, "unstirred" incubation | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("in the absence of organic solvents except the very small quantity taken with the 50-microL aliquot")/Deducted organic solvent volume (annotated as "saturating concentration") |
| Stability - Incubation | Activity after incubation (3 days at 25Β°C), measured by absorbance spectrophotometry (benzoyl-L-tyrosine absorbance measurement, 258 nm) in aqueous phase | Ethyl Acetate | 7% | 100 | 100 | % | 0.1 M phosphate buffer | 7 | 25Β°C | 0.4 mM N-Benzoyl-L-tyrosine ethyl ester | Ethanol , N-Benzoyl-L-tyrosine | β | No, "unstirred" incubation | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("in the absence of organic solvents except the very small quantity taken with the 50-microL aliquot")/Deducted organic solvent volume (annotated as "saturating concentration") |
| Stability - Incubation | Activity after incubation (15 days at 25Β°C), measured by absorbance spectrophotometry (benzoyl-L-tyrosine absorbance measurement, 258 nm) in aqueous phase | Dichloromethane | 1.5% | 100 | 28 | % | 0.1 M phosphate buffer | 7 | 25Β°C | 0.4 mM N-Benzoyl-L-tyrosine ethyl ester | Ethanol , N-Benzoyl-L-tyrosine | β | No, "unstirred" incubation | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("in the absence of organic solvents except the very small quantity taken with the 50-microL aliquot")/Deducted organic solvent volume (annotated as "saturating concentration") |
| Stability - Incubation | Activity after incubation (10 days at 25Β°C), measured by absorbance spectrophotometry (benzoyl-L-tyrosine absorbance measurement, 258 nm) in aqueous phase | Dichloromethane | 1.5% | 100 | 53 | % | 0.1 M phosphate buffer | 7 | 25Β°C | 0.4 mM N-Benzoyl-L-tyrosine ethyl ester | Ethanol , N-Benzoyl-L-tyrosine | β | No, "unstirred" incubation | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("in the absence of organic solvents except the very small quantity taken with the 50-microL aliquot")/Deducted organic solvent volume (annotated as "saturating concentration") |
| Stability - Incubation | Activity after incubation (5 days at 25Β°C), measured by absorbance spectrophotometry (benzoyl-L-tyrosine absorbance measurement, 258 nm) in aqueous phase | Dichloromethane | 1.5% | 100 | 83 | % | 0.1 M phosphate buffer | 7 | 25Β°C | 0.4 mM N-Benzoyl-L-tyrosine ethyl ester | Ethanol , N-Benzoyl-L-tyrosine | β | No, "unstirred" incubation | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("in the absence of organic solvents except the very small quantity taken with the 50-microL aliquot")/Deducted organic solvent volume (annotated as "saturating concentration") |
| Stability - Incubation | Activity after incubation (4 days at 25Β°C), measured by absorbance spectrophotometry (benzoyl-L-tyrosine absorbance measurement, 258 nm) in aqueous phase | Dichloromethane | 1.5% | 100 | 93 | % | 0.1 M phosphate buffer | 7 | 25Β°C | 0.4 mM N-Benzoyl-L-tyrosine ethyl ester | Ethanol , N-Benzoyl-L-tyrosine | β | No, "unstirred" incubation | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("in the absence of organic solvents except the very small quantity taken with the 50-microL aliquot")/Deducted organic solvent volume (annotated as "saturating concentration") |
| Stability - Incubation | Activity after incubation (2 days at 25Β°C), measured by absorbance spectrophotometry (benzoyl-L-tyrosine absorbance measurement, 258 nm) in aqueous phase | Dichloromethane | 1.5% | 100 | 100 | % | 0.1 M phosphate buffer | 7 | 25Β°C | 0.4 mM N-Benzoyl-L-tyrosine ethyl ester | Ethanol , N-Benzoyl-L-tyrosine | β | No, "unstirred" incubation | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("in the absence of organic solvents except the very small quantity taken with the 50-microL aliquot")/Deducted organic solvent volume (annotated as "saturating concentration") |
Visualization : Stability β Incubation
Rosa M. Blanco et al. (1992)
One bar per measurement. Colour = solvent, shade = solvent volume.
Hiroyasu Ogino et al. (1999)
β Purification and characterization of organic solvent-stable protease from organic solvent-tolerant Pseudomonas aeruginosa PST-01
Journal of Bioscience and Bioengineering
Β· doi:10.1016/s1389-1723(99)80009-7 β
Β· Stability - Half-life
▼
Database ID
UniProt: P00766 β
Sequence Annotation
Inferred - from protein name
Protein Source
Commercially purchased
Experimental Data (16 measurements)
16 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Half-life | Half-life (in days) with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase | Ethylene Glycol | 25% | 13.2 | 6.9 | days | Incubation: 10 mM Borax-HCl buffer, Assay: 50 mM Borax-HCl buffer | Incubation: 8.5, Assay: 8.5 | Incubation: 30Β°C, Assay: 30Β°C | 0.6% (w/v) Casein | free amino acids , Peptides | β | Incubation: 160 rpm | Classical aqueous control (in days) |
| Stability - Half-life | Half-life (in days) with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase | 1,4-Butanediol | 25% | 13.2 | 0.7 | days | Incubation: 10 mM Borax-HCl buffer, Assay: 50 mM Borax-HCl buffer | Incubation: 8.5, Assay: 8.5 | Incubation: 30Β°C, Assay: 30Β°C | 0.6% (w/v) Casein | free amino acids , Peptides | β | Incubation: 160 rpm | Classical aqueous control (in days) |
| Stability - Half-life | Half-life (in days) with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase | 1,5-Pentanediol | 25% | 13.2 | 0.4 | days | Incubation: 10 mM Borax-HCl buffer, Assay: 50 mM Borax-HCl buffer | Incubation: 8.5, Assay: 8.5 | Incubation: 30Β°C, Assay: 30Β°C | 0.6% (w/v) Casein | free amino acids , Peptides | β | Incubation: 160 rpm | Classical aqueous control (in days) |
| Stability - Half-life | Half-life (in days) with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase | Ethanol | 25% | 13.2 | 27.0 | days | Incubation: 10 mM Borax-HCl buffer, Assay: 50 mM Borax-HCl buffer | Incubation: 8.5, Assay: 8.5 | Incubation: 30Β°C, Assay: 30Β°C | 0.6% (w/v) Casein | free amino acids , Peptides | β | Incubation: 160 rpm | Classical aqueous control (in days) |
| Stability - Half-life | Half-life (in days) with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase | 1-Hexanol | 25% | 13.2 | 14.2 | days | Incubation: 10 mM Borax-HCl buffer, Assay: 50 mM Borax-HCl buffer | Incubation: 8.5, Assay: 8.5 | Incubation: 30Β°C, Assay: 30Β°C | 0.6% (w/v) Casein | free amino acids , Peptides | β | Incubation: 160 rpm | Classical aqueous control (in days) |
| Stability - Half-life | Half-life (in days) with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase | Methanol | 25% | 13.2 | 6.0 | days | Incubation: 10 mM Borax-HCl buffer, Assay: 50 mM Borax-HCl buffer | Incubation: 8.5, Assay: 8.5 | Incubation: 30Β°C, Assay: 30Β°C | 0.6% (w/v) Casein | free amino acids , Peptides | β | Incubation: 160 rpm | Classical aqueous control (in days) |
| Stability - Half-life | Half-life (in days) with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 25% | 13.2 | 33.6 | days | Incubation: 10 mM Borax-HCl buffer, Assay: 50 mM Borax-HCl buffer | Incubation: 8.5, Assay: 8.5 | Incubation: 30Β°C, Assay: 30Β°C | 0.6% (w/v) Casein | free amino acids , Peptides | β | Incubation: 160 rpm | Classical aqueous control (in days) |
| Stability - Half-life | Half-life (in days) with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase | Isopropanol | 25% | 13.2 | 0.6 | days | Incubation: 10 mM Borax-HCl buffer, Assay: 50 mM Borax-HCl buffer | Incubation: 8.5, Assay: 8.5 | Incubation: 30Β°C, Assay: 30Β°C | 0.6% (w/v) Casein | free amino acids , Peptides | β | Incubation: 160 rpm | Classical aqueous control (in days) |
| Stability - Half-life | Half-life (in days) with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase | Triethylene Glycol | 25% | 13.2 | 11.1 | days | Incubation: 10 mM Borax-HCl buffer, Assay: 50 mM Borax-HCl buffer | Incubation: 8.5, Assay: 8.5 | Incubation: 30Β°C, Assay: 30Β°C | 0.6% (w/v) Casein | free amino acids , Peptides | β | Incubation: 160 rpm | Classical aqueous control (in days) |
| Stability - Half-life | Half-life (in days) with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase | tert-Butanol | 25% | 13.2 | 0.5 | days | Incubation: 10 mM Borax-HCl buffer, Assay: 50 mM Borax-HCl buffer | Incubation: 8.5, Assay: 8.5 | Incubation: 30Β°C, Assay: 30Β°C | 0.6% (w/v) Casein | free amino acids , Peptides | β | Incubation: 160 rpm | Classical aqueous control (in days) |
| Stability - Half-life | Half-life (in days) with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase | 1-Heptanol | 25% | 13.2 | 3.8 | days | Incubation: 10 mM Borax-HCl buffer, Assay: 50 mM Borax-HCl buffer | Incubation: 8.5, Assay: 8.5 | Incubation: 30Β°C, Assay: 30Β°C | 0.6% (w/v) Casein | free amino acids , Peptides | β | Incubation: 160 rpm | Classical aqueous control (in days) |
| Stability - Half-life | Half-life (in days) with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase | Dimethylformamide (DMF) | 25% | 13.2 | 2.2 | days | Incubation: 10 mM Borax-HCl buffer, Assay: 50 mM Borax-HCl buffer | Incubation: 8.5, Assay: 8.5 | Incubation: 30Β°C, Assay: 30Β°C | 0.6% (w/v) Casein | free amino acids , Peptides | β | Incubation: 160 rpm | Classical aqueous control (in days) |
| Stability - Half-life | Half-life (in days) with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase | 1-Butanol | 25% | 13.2 | 0.3 | days | Incubation: 10 mM Borax-HCl buffer, Assay: 50 mM Borax-HCl buffer | Incubation: 8.5, Assay: 8.5 | Incubation: 30Β°C, Assay: 30Β°C | 0.6% (w/v) Casein | free amino acids , Peptides | β | Incubation: 160 rpm | Classical aqueous control (in days) |
| Stability - Half-life | Half-life (in days) with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase | Acetone | 25% | 13.2 | 0.6 | days | Incubation: 10 mM Borax-HCl buffer, Assay: 50 mM Borax-HCl buffer | Incubation: 8.5, Assay: 8.5 | Incubation: 30Β°C, Assay: 30Β°C | 0.6% (w/v) Casein | free amino acids , Peptides | β | Incubation: 160 rpm | Classical aqueous control (in days) |
| Stability - Half-life | Half-life (in days) with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase | 1,4-Dioxane | 25% | 13.2 | 0.5 | days | Incubation: 10 mM Borax-HCl buffer, Assay: 50 mM Borax-HCl buffer | Incubation: 8.5, Assay: 8.5 | Incubation: 30Β°C, Assay: 30Β°C | 0.6% (w/v) Casein | free amino acids , Peptides | β | Incubation: 160 rpm | Classical aqueous control (in days) |
| Stability - Half-life | Half-life (in days) with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase | Toluene | 25% | 13.2 | >100.0 | days | Incubation: 10 mM Borax-HCl buffer, Assay: 50 mM Borax-HCl buffer | Incubation: 8.5, Assay: 8.5 | Incubation: 30Β°C, Assay: 30Β°C | 0.6% (w/v) Casein | free amino acids , Peptides | β | Incubation: 160 rpm | Classical aqueous control (in days) |
Visualization : Stability β Half-life
Hiroyasu Ogino et al. (1999)
One bar per measurement. Colour = solvent, shade = solvent volume.
Guillermo R. Castro et al. (1999)
β Enzymatic activities of proteases dissolved in organic solvents
Enzyme and Microbial Technology
Β· doi:10.1016/S0141-0229(99)00099-X β
Β· Activity - Michaelis-Menten
▼
Database ID
UniProt: P00766 β
Sequence Annotation
Inferred - from protein name
Protein Source
Commercially purchased
Experimental Data (3 measurements)
3 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Michaelis-Menten | Km (mM) measured by HPLC in the presence of organic solvent | Glycerol | 99% | 4.4 | 41.9 | mM | 20 mM phosphate buffer | 7.8 | 30Β°C | Phenyl acetate | Acetic Acid , Phenol | β | β | Classical aqueous control (in mM) |
| Activity - Michaelis-Menten | Vmax (Β΅M/s mg) measured by HPLC in the presence of organic solvent | Glycerol | 99% | 0.38 | 0.2 | Β΅M/(s * mg) | 20 mM phosphate buffer | 7.8 | 30Β°C | Phenyl acetate | Acetic Acid , Phenol | β | β | Classical aqueous control (in microM/(s * mg)) |
| Activity - Michaelis-Menten | Vm/Km (1/s mg) measured by HPLC in the presence of organic solvent | Glycerol | 99% | 0.086 | 0.0048 | 1/(s * mg) | 20 mM phosphate buffer | 7.8 | 30Β°C | Phenyl acetate | Acetic Acid , Phenol | β | β | Classical aqueous control (in 1/(s * mg)) |
Visualization : Activity β Michaelis-Menten
Guillermo R. Castro et al. (1999)
One bar per measurement. Colour = solvent, shade = solvent volume.
L.M. Simon et al. (2001)
β Structure and Activity of Ξ±-Chymotrypsin and Trypsin in Aqueous Organic Media.
Biochemical and Biophysical Research Communications
Β· doi:10.1006/bbrc.2001.4282 β
Β· Activity - Michaelis-Menten Stability - Incubation
▼
Database ID
UniProt: P00766 β
Sequence Annotation
Inferred - from protein name
Protein Source
Commercially purchased
Experimental Data (53 measurements)
53 measurements β page 1 of 3
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Michaelis-Menten | Km (10^-2 * mM) measured by absorbance spectrophotometry (N-Acetyl-L-tyrosine absorbance measurement, 237 nm) in the presence of organic solvent | Ethanol | 50% | 1.07 | 0.65 | 10^-2 * mM | 40 mM Tris-HCl | 7 | 25Β°C | N-Acetyl-L-tyrosine ethyl ester | Ethanol , N-Acetyl-L-tyrosine | β | β | Classical aqueous control (in 10^-2 * mM) |
| Activity - Michaelis-Menten | Km (10^-2 * mM) measured by absorbance spectrophotometry (N-Acetyl-L-tyrosine absorbance measurement, 237 nm) in the presence of organic solvent | Ethanol | 20% | 1.07 | 0.62 | 10^-2 * mM | 40 mM Tris-HCl | 7 | 25Β°C | N-Acetyl-L-tyrosine ethyl ester | Ethanol , N-Acetyl-L-tyrosine | β | β | Classical aqueous control (in 10^-2 * mM) |
| Activity - Michaelis-Menten | Km (10^-2 * mM) measured by absorbance spectrophotometry (N-Acetyl-L-tyrosine absorbance measurement, 237 nm) in the presence of organic solvent | Acetonitrile | 95% | 1.07 | 0.56 | 10^-2 * mM | 40 mM Tris-HCl | 7 | 25Β°C | N-Acetyl-L-tyrosine ethyl ester | Ethanol , N-Acetyl-L-tyrosine | β | β | Classical aqueous control (in 10^-2 * mM) |
| Activity - Michaelis-Menten | Km (10^-2 * mM) measured by absorbance spectrophotometry (N-Acetyl-L-tyrosine absorbance measurement, 237 nm) in the presence of organic solvent | Acetonitrile | 80% | 1.07 | 0.42 | 10^-2 * mM | 40 mM Tris-HCl | 7 | 25Β°C | N-Acetyl-L-tyrosine ethyl ester | Ethanol , N-Acetyl-L-tyrosine | β | β | Classical aqueous control (in 10^-2 * mM) |
| Activity - Michaelis-Menten | Km (10^-2 * mM) measured by absorbance spectrophotometry (N-Acetyl-L-tyrosine absorbance measurement, 237 nm) in the presence of organic solvent | Acetonitrile | 50% | 1.07 | 0.34 | 10^-2 * mM | 40 mM Tris-HCl | 7 | 25Β°C | N-Acetyl-L-tyrosine ethyl ester | Ethanol , N-Acetyl-L-tyrosine | β | β | Classical aqueous control (in 10^-2 * mM) |
| Activity - Michaelis-Menten | Km (10^-2 * mM) measured by absorbance spectrophotometry (N-Acetyl-L-tyrosine absorbance measurement, 237 nm) in the presence of organic solvent | Acetonitrile | 20% | 1.07 | 0.38 | 10^-2 * mM | 40 mM Tris-HCl | 7 | 25Β°C | N-Acetyl-L-tyrosine ethyl ester | Ethanol , N-Acetyl-L-tyrosine | β | β | Classical aqueous control (in 10^-2 * mM) |
| Activity - Michaelis-Menten | Km (10^-2 * mM) measured by absorbance spectrophotometry (N-Acetyl-L-tyrosine absorbance measurement, 237 nm) in the presence of organic solvent | 1,4-Dioxane | 95% | 1.07 | 0.55 | 10^-2 * mM | 40 mM Tris-HCl | 7 | 25Β°C | N-Acetyl-L-tyrosine ethyl ester | Ethanol , N-Acetyl-L-tyrosine | β | β | Classical aqueous control (in 10^-2 * mM) |
| Activity - Michaelis-Menten | Km (10^-2 * mM) measured by absorbance spectrophotometry (N-Acetyl-L-tyrosine absorbance measurement, 237 nm) in the presence of organic solvent | 1,4-Dioxane | 80% | 1.07 | 0.39 | 10^-2 * mM | 40 mM Tris-HCl | 7 | 25Β°C | N-Acetyl-L-tyrosine ethyl ester | Ethanol , N-Acetyl-L-tyrosine | β | β | Classical aqueous control (in 10^-2 * mM) |
| Activity - Michaelis-Menten | Km (10^-2 * mM) measured by absorbance spectrophotometry (N-Acetyl-L-tyrosine absorbance measurement, 237 nm) in the presence of organic solvent | 1,4-Dioxane | 50% | 1.07 | 0.24 | 10^-2 * mM | 40 mM Tris-HCl | 7 | 25Β°C | N-Acetyl-L-tyrosine ethyl ester | Ethanol , N-Acetyl-L-tyrosine | β | β | Classical aqueous control (in 10^-2 * mM) |
| Activity - Michaelis-Menten | Km (10^-2 * mM) measured by absorbance spectrophotometry (N-Acetyl-L-tyrosine absorbance measurement, 237 nm) in the presence of organic solvent | 1,4-Dioxane | 20% | 1.07 | 0.34 | 10^-2 * mM | 40 mM Tris-HCl | 7 | 25Β°C | N-Acetyl-L-tyrosine ethyl ester | Ethanol , N-Acetyl-L-tyrosine | β | β | Classical aqueous control (in 10^-2 * mM) |
| Activity - Michaelis-Menten | Km (10^-2 * mM) measured by absorbance spectrophotometry (N-Acetyl-L-tyrosine absorbance measurement, 237 nm) in the presence of organic solvent | Ethanol | 95% | 1.07 | 0.65 | 10^-2 * mM | 40 mM Tris-HCl | 7 | 25Β°C | N-Acetyl-L-tyrosine ethyl ester | Ethanol , N-Acetyl-L-tyrosine | β | β | Classical aqueous control (in 10^-2 * mM) |
| Activity - Michaelis-Menten | Km (10^-2 * mM) measured by absorbance spectrophotometry (N-Acetyl-L-tyrosine absorbance measurement, 237 nm) in the presence of organic solvent | Ethanol | 80% | 1.07 | 0.63 | 10^-2 * mM | 40 mM Tris-HCl | 7 | 25Β°C | N-Acetyl-L-tyrosine ethyl ester | Ethanol , N-Acetyl-L-tyrosine | β | β | Classical aqueous control (in 10^-2 * mM) |
| Stability - Incubation | Activity after incubation (20 minutes at 25Β°C), measured by absorbance spectrophotometry (N-Acetyl-L-tyrosine absorbance measurement, 237 nm) in aqueous phase | 1,4-Dioxane | 70% | 100.0 | 0.0 | % | Incubation: Distilled water, Assay: 40 mM Tris-HCl | Incubation: 3, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 0.5 mM N-Acetyl-L-tyrosine ethyl ester | Ethanol , N-Acetyl-L-tyrosine | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (20 minutes at 25Β°C), measured by absorbance spectrophotometry (N-Acetyl-L-tyrosine absorbance measurement, 237 nm) in aqueous phase | Acetonitrile | 95% | 100.0 | 64.0 | % | Incubation: Distilled water, Assay: 40 mM Tris-HCl | Incubation: 3, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 0.5 mM N-Acetyl-L-tyrosine ethyl ester | Ethanol , N-Acetyl-L-tyrosine | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (20 minutes at 25Β°C), measured by absorbance spectrophotometry (N-Acetyl-L-tyrosine absorbance measurement, 237 nm) in aqueous phase | 1,4-Dioxane | 75% | 100.0 | 0.0 | % | Incubation: Distilled water, Assay: 40 mM Tris-HCl | Incubation: 3, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 0.5 mM N-Acetyl-L-tyrosine ethyl ester | Ethanol , N-Acetyl-L-tyrosine | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (20 minutes at 25Β°C), measured by absorbance spectrophotometry (N-Acetyl-L-tyrosine absorbance measurement, 237 nm) in aqueous phase | 1,4-Dioxane | 80% | 100.0 | 0.0 | % | Incubation: Distilled water, Assay: 40 mM Tris-HCl | Incubation: 3, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 0.5 mM N-Acetyl-L-tyrosine ethyl ester | Ethanol , N-Acetyl-L-tyrosine | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (20 minutes at 25Β°C), measured by absorbance spectrophotometry (N-Acetyl-L-tyrosine absorbance measurement, 237 nm) in aqueous phase | 1,4-Dioxane | 85% | 100.0 | 0.0 | % | Incubation: Distilled water, Assay: 40 mM Tris-HCl | Incubation: 3, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 0.5 mM N-Acetyl-L-tyrosine ethyl ester | Ethanol , N-Acetyl-L-tyrosine | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (20 minutes at 25Β°C), measured by absorbance spectrophotometry (N-Acetyl-L-tyrosine absorbance measurement, 237 nm) in aqueous phase | 1,4-Dioxane | 90% | 100.0 | 0.0 | % | Incubation: Distilled water, Assay: 40 mM Tris-HCl | Incubation: 3, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 0.5 mM N-Acetyl-L-tyrosine ethyl ester | Ethanol , N-Acetyl-L-tyrosine | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (20 minutes at 25Β°C), measured by absorbance spectrophotometry (N-Acetyl-L-tyrosine absorbance measurement, 237 nm) in aqueous phase | 1,4-Dioxane | 95% | 100.0 | 18.0 | % | Incubation: Distilled water, Assay: 40 mM Tris-HCl | Incubation: 3, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 0.5 mM N-Acetyl-L-tyrosine ethyl ester | Ethanol , N-Acetyl-L-tyrosine | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (20 minutes at 25Β°C), measured by absorbance spectrophotometry (N-Acetyl-L-tyrosine absorbance measurement, 237 nm) in aqueous phase | Acetonitrile | 10% | 100.0 | 94.0 | % | Incubation: Distilled water, Assay: 40 mM Tris-HCl | Incubation: 3, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 0.5 mM N-Acetyl-L-tyrosine ethyl ester | Ethanol , N-Acetyl-L-tyrosine | β | β | Non-incubated control (in %), assay in aqueous phase |
Visualization : Activity β Michaelis-Menten
L.M. Simon et al. (2001)
One bar per measurement. Colour = solvent, shade = solvent volume.
Visualization : Stability β Incubation
L.M. Simon et al. (2001)
One bar per measurement. Colour = solvent, shade = solvent volume.
E. A. Belyaeva et al. (2002)
β On the Mechanism of Interaction of Organic Solvents with the Active Site of Ξ±-Chymotrypsin
Biochemistry (Moscow)
Β· doi:10.1023/a:1020530220774 β
Β· Activity - Michaelis-Menten
▼
Database ID
UniProt: P00766 β
Sequence Annotation
Inferred - from protein name
Protein Source
Commercially purchased
Experimental Data (31 measurements)
31 measurements β page 2 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Michaelis-Menten | Kcat (in /s) measured by pH-stat in the presence of organic solvent | Ethanol | 0.35M | 0.04 | 0.067 | 1/s | 0.05 M sodium phosphate | 8 | 20Β°C | N-Acetyl-L-tyrosine ethyl ester | Ethanol , N-Acetyl-L-tyrosine | β | β | Classical aqueous control (in 1/s) |
| Activity - Michaelis-Menten | Kcat (in /s) measured by pH-stat in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 3.6 M | 0.04 | 0.036 | 1/s | 0.05 M sodium phosphate | 8 | 20Β°C | N-Acetyl-L-tyrosine ethyl ester | Ethanol , N-Acetyl-L-tyrosine | β | β | Classical aqueous control (in 1/s) |
| Activity - Michaelis-Menten | Kcat (in /s) measured by pH-stat in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 3.2 M | 0.04 | 0.039 | 1/s | 0.05 M sodium phosphate | 8 | 20Β°C | N-Acetyl-L-tyrosine ethyl ester | Ethanol , N-Acetyl-L-tyrosine | β | β | Classical aqueous control (in 1/s) |
| Activity - Michaelis-Menten | Kcat (in /s) measured by pH-stat in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 2.9 M | 0.04 | 0.038 | 1/s | 0.05 M sodium phosphate | 8 | 20Β°C | N-Acetyl-L-tyrosine ethyl ester | Ethanol , N-Acetyl-L-tyrosine | β | β | Classical aqueous control (in 1/s) |
| Activity - Michaelis-Menten | Kcat (in /s) measured by pH-stat in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 2.5 M | 0.04 | 0.054 | 1/s | 0.05 M sodium phosphate | 8 | 20Β°C | N-Acetyl-L-tyrosine ethyl ester | Ethanol , N-Acetyl-L-tyrosine | β | β | Classical aqueous control (in 1/s) |
| Activity - Michaelis-Menten | Kcat (in /s) measured by pH-stat in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 2.2 M | 0.04 | 0.074 | 1/s | 0.05 M sodium phosphate | 8 | 20Β°C | N-Acetyl-L-tyrosine ethyl ester | Ethanol , N-Acetyl-L-tyrosine | β | β | Classical aqueous control (in 1/s) |
| Activity - Michaelis-Menten | Kcat (in /s) measured by pH-stat in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 1.5 M | 0.04 | 0.088 | 1/s | 0.05 M sodium phosphate | 8 | 20Β°C | N-Acetyl-L-tyrosine ethyl ester | Ethanol , N-Acetyl-L-tyrosine | β | β | Classical aqueous control (in 1/s) |
| Activity - Michaelis-Menten | Kcat (in /s) measured by pH-stat in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 1 M | 0.04 | 0.08 | 1/s | 0.05 M sodium phosphate | 8 | 20Β°C | N-Acetyl-L-tyrosine ethyl ester | Ethanol , N-Acetyl-L-tyrosine | β | β | Classical aqueous control (in 1/s) |
| Activity - Michaelis-Menten | Kcat (in /s) measured by pH-stat in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 0.75 M | 0.04 | 0.085 | 1/s | 0.05 M sodium phosphate | 8 | 20Β°C | N-Acetyl-L-tyrosine ethyl ester | Ethanol , N-Acetyl-L-tyrosine | β | β | Classical aqueous control (in 1/s) |
| Activity - Michaelis-Menten | Kcat (in /s) measured by pH-stat in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 0.45 M | 0.04 | 0.07 | 1/s | 0.05 M sodium phosphate | 8 | 20Β°C | N-Acetyl-L-tyrosine ethyl ester | Ethanol , N-Acetyl-L-tyrosine | β | β | Classical aqueous control (in 1/s) |
| Activity - Michaelis-Menten | Kcat (in /s) measured by pH-stat in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 0.3 M | 0.04 | 0.067 | 1/s | 0.05 M sodium phosphate | 8 | 20Β°C | N-Acetyl-L-tyrosine ethyl ester | Ethanol , N-Acetyl-L-tyrosine | β | β | Classical aqueous control (in 1/s) |
Visualization : Activity β Michaelis-Menten
E. A. Belyaeva et al. (2002)
One bar per measurement. Colour = solvent, shade = solvent volume.
Linus Olofsson et al. (2006)
β Influence of Water Miscible Organic Solvents on Ξ±-chymotrypsin in Solution and Immobilized on Eupergit CM
Biotechnology Letters
Β· doi:10.1007/s10529-006-9025-7 β
Β· Activity + Stability - Incubation
▼
Database ID
UniProt: P00766 β
Sequence Annotation
Inferred - from protein name
Protein Source
Commercially purchased
Experimental Data (45 measurements)
45 measurements β page 1 of 3
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity + Stability - Incubation | Activity after incubation (6 hours at 20Β°C), measured by absorbance spectrophotometry (colorimetric assay, Ξ²-naphtol and Fast Blue B salt reaction product absorbance measurement, 540 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 30% | 100 | 736 | % | Incubation: 0.1M sodium phosphate, Assay: 0.1M sodium phosphate, 0.75 mg of Fast blue B salt | Incubation: 7.8, Assay: 7.8 | Incubation: 20Β°C, Assay: 20Β°C | 0.75 mg Acetyl-DL-phenylalanine Ξ²-naphthyl ester | N-Acetyl-DL-phenylalanine , Ξ²-Naphthol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Activity + Stability - Incubation | Activity after incubation (6 hours at 20Β°C), measured by absorbance spectrophotometry (colorimetric assay, Ξ²-naphtol and Fast Blue B salt reaction product absorbance measurement, 540 nm) in the presence of organic solvent | Acetonitrile | 50% | 100 | 0 | % | Incubation: 0.1M sodium phosphate, Assay: 0.1M sodium phosphate, 0.75 mg of Fast blue B salt | Incubation: 7.8, Assay: 7.8 | Incubation: 20Β°C, Assay: 20Β°C | 0.75 mg Acetyl-DL-phenylalanine Ξ²-naphthyl ester | N-Acetyl-DL-phenylalanine , Ξ²-Naphthol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Activity + Stability - Incubation | Activity after incubation (6 hours at 20Β°C), measured by absorbance spectrophotometry (colorimetric assay, Ξ²-naphtol and Fast Blue B salt reaction product absorbance measurement, 540 nm) in the presence of organic solvent | Acetonitrile | 55% | 100 | 0 | % | Incubation: 0.1M sodium phosphate, Assay: 0.1M sodium phosphate, 0.75 mg of Fast blue B salt | Incubation: 7.8, Assay: 7.8 | Incubation: 20Β°C, Assay: 20Β°C | 0.75 mg Acetyl-DL-phenylalanine Ξ²-naphthyl ester | N-Acetyl-DL-phenylalanine , Ξ²-Naphthol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Activity + Stability - Incubation | Activity after incubation (6 hours at 20Β°C), measured by absorbance spectrophotometry (colorimetric assay, Ξ²-naphtol and Fast Blue B salt reaction product absorbance measurement, 540 nm) in the presence of organic solvent | Acetonitrile | 60% | 100 | 22 | % | Incubation: 0.1M sodium phosphate, Assay: 0.1M sodium phosphate, 0.75 mg of Fast blue B salt | Incubation: 7.8, Assay: 7.8 | Incubation: 20Β°C, Assay: 20Β°C | 0.75 mg Acetyl-DL-phenylalanine Ξ²-naphthyl ester | N-Acetyl-DL-phenylalanine , Ξ²-Naphthol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Activity + Stability - Incubation | Activity after incubation (6 hours at 20Β°C), measured by absorbance spectrophotometry (colorimetric assay, Ξ²-naphtol and Fast Blue B salt reaction product absorbance measurement, 540 nm) in the presence of organic solvent | Acetonitrile | 70% | 100 | 0 | % | Incubation: 0.1M sodium phosphate, Assay: 0.1M sodium phosphate, 0.75 mg of Fast blue B salt | Incubation: 7.8, Assay: 7.8 | Incubation: 20Β°C, Assay: 20Β°C | 0.75 mg Acetyl-DL-phenylalanine Ξ²-naphthyl ester | N-Acetyl-DL-phenylalanine , Ξ²-Naphthol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Activity + Stability - Incubation | Activity after incubation (6 hours at 20Β°C), measured by absorbance spectrophotometry (colorimetric assay, Ξ²-naphtol and Fast Blue B salt reaction product absorbance measurement, 540 nm) in the presence of organic solvent | Acetonitrile | 80% | 100 | 0 | % | Incubation: 0.1M sodium phosphate, Assay: 0.1M sodium phosphate, 0.75 mg of Fast blue B salt | Incubation: 7.8, Assay: 7.8 | Incubation: 20Β°C, Assay: 20Β°C | 0.75 mg Acetyl-DL-phenylalanine Ξ²-naphthyl ester | N-Acetyl-DL-phenylalanine , Ξ²-Naphthol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Activity + Stability - Incubation | Activity after incubation (6 hours at 20Β°C), measured by absorbance spectrophotometry (colorimetric assay, Ξ²-naphtol and Fast Blue B salt reaction product absorbance measurement, 540 nm) in the presence of organic solvent | Acetonitrile | 90% | 100 | 0 | % | Incubation: 0.1M sodium phosphate, Assay: 0.1M sodium phosphate, 0.75 mg of Fast blue B salt | Incubation: 7.8, Assay: 7.8 | Incubation: 20Β°C, Assay: 20Β°C | 0.75 mg Acetyl-DL-phenylalanine Ξ²-naphthyl ester | N-Acetyl-DL-phenylalanine , Ξ²-Naphthol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Activity + Stability - Incubation | Activity after incubation (6 hours at 20Β°C), measured by absorbance spectrophotometry (colorimetric assay, Ξ²-naphtol and Fast Blue B salt reaction product absorbance measurement, 540 nm) in the presence of organic solvent | Acetonitrile | 95% | 100 | 0 | % | Incubation: 0.1M sodium phosphate, Assay: 0.1M sodium phosphate, 0.75 mg of Fast blue B salt | Incubation: 7.8, Assay: 7.8 | Incubation: 20Β°C, Assay: 20Β°C | 0.75 mg Acetyl-DL-phenylalanine Ξ²-naphthyl ester | N-Acetyl-DL-phenylalanine , Ξ²-Naphthol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Activity + Stability - Incubation | Activity after incubation (6 hours at 20Β°C), measured by absorbance spectrophotometry (colorimetric assay, Ξ²-naphtol and Fast Blue B salt reaction product absorbance measurement, 540 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 10% | 100 | 317 | % | Incubation: 0.1M sodium phosphate, Assay: 0.1M sodium phosphate, 0.75 mg of Fast blue B salt | Incubation: 7.8, Assay: 7.8 | Incubation: 20Β°C, Assay: 20Β°C | 0.75 mg Acetyl-DL-phenylalanine Ξ²-naphthyl ester | N-Acetyl-DL-phenylalanine , Ξ²-Naphthol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Activity + Stability - Incubation | Activity after incubation (6 hours at 20Β°C), measured by absorbance spectrophotometry (colorimetric assay, Ξ²-naphtol and Fast Blue B salt reaction product absorbance measurement, 540 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 15% | 100 | 529 | % | Incubation: 0.1M sodium phosphate, Assay: 0.1M sodium phosphate, 0.75 mg of Fast blue B salt | Incubation: 7.8, Assay: 7.8 | Incubation: 20Β°C, Assay: 20Β°C | 0.75 mg Acetyl-DL-phenylalanine Ξ²-naphthyl ester | N-Acetyl-DL-phenylalanine , Ξ²-Naphthol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Activity + Stability - Incubation | Activity after incubation (6 hours at 20Β°C), measured by absorbance spectrophotometry (colorimetric assay, Ξ²-naphtol and Fast Blue B salt reaction product absorbance measurement, 540 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 20% | 100 | 690 | % | Incubation: 0.1M sodium phosphate, Assay: 0.1M sodium phosphate, 0.75 mg of Fast blue B salt | Incubation: 7.8, Assay: 7.8 | Incubation: 20Β°C, Assay: 20Β°C | 0.75 mg Acetyl-DL-phenylalanine Ξ²-naphthyl ester | N-Acetyl-DL-phenylalanine , Ξ²-Naphthol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Activity + Stability - Incubation | Activity after incubation (6 hours at 20Β°C), measured by absorbance spectrophotometry (colorimetric assay, Ξ²-naphtol and Fast Blue B salt reaction product absorbance measurement, 540 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 25% | 100 | 782 | % | Incubation: 0.1M sodium phosphate, Assay: 0.1M sodium phosphate, 0.75 mg of Fast blue B salt | Incubation: 7.8, Assay: 7.8 | Incubation: 20Β°C, Assay: 20Β°C | 0.75 mg Acetyl-DL-phenylalanine Ξ²-naphthyl ester | N-Acetyl-DL-phenylalanine , Ξ²-Naphthol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Activity + Stability - Incubation | Activity after incubation (6 hours at 20Β°C), measured by absorbance spectrophotometry (colorimetric assay, Ξ²-naphtol and Fast Blue B salt reaction product absorbance measurement, 540 nm) in the presence of organic solvent | Acetonitrile | 45% | 100 | 0 | % | Incubation: 0.1M sodium phosphate, Assay: 0.1M sodium phosphate, 0.75 mg of Fast blue B salt | Incubation: 7.8, Assay: 7.8 | Incubation: 20Β°C, Assay: 20Β°C | 0.75 mg Acetyl-DL-phenylalanine Ξ²-naphthyl ester | N-Acetyl-DL-phenylalanine , Ξ²-Naphthol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Activity + Stability - Incubation | Activity after incubation (6 hours at 20Β°C), measured by absorbance spectrophotometry (colorimetric assay, Ξ²-naphtol and Fast Blue B salt reaction product absorbance measurement, 540 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 35% | 100 | 625 | % | Incubation: 0.1M sodium phosphate, Assay: 0.1M sodium phosphate, 0.75 mg of Fast blue B salt | Incubation: 7.8, Assay: 7.8 | Incubation: 20Β°C, Assay: 20Β°C | 0.75 mg Acetyl-DL-phenylalanine Ξ²-naphthyl ester | N-Acetyl-DL-phenylalanine , Ξ²-Naphthol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Activity + Stability - Incubation | Activity after incubation (6 hours at 20Β°C), measured by absorbance spectrophotometry (colorimetric assay, Ξ²-naphtol and Fast Blue B salt reaction product absorbance measurement, 540 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 40% | 100 | 377 | % | Incubation: 0.1M sodium phosphate, Assay: 0.1M sodium phosphate, 0.75 mg of Fast blue B salt | Incubation: 7.8, Assay: 7.8 | Incubation: 20Β°C, Assay: 20Β°C | 0.75 mg Acetyl-DL-phenylalanine Ξ²-naphthyl ester | N-Acetyl-DL-phenylalanine , Ξ²-Naphthol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Activity + Stability - Incubation | Activity after incubation (6 hours at 20Β°C), measured by absorbance spectrophotometry (colorimetric assay, Ξ²-naphtol and Fast Blue B salt reaction product absorbance measurement, 540 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 45% | 100 | 74 | % | Incubation: 0.1M sodium phosphate, Assay: 0.1M sodium phosphate, 0.75 mg of Fast blue B salt | Incubation: 7.8, Assay: 7.8 | Incubation: 20Β°C, Assay: 20Β°C | 0.75 mg Acetyl-DL-phenylalanine Ξ²-naphthyl ester | N-Acetyl-DL-phenylalanine , Ξ²-Naphthol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Activity + Stability - Incubation | Activity after incubation (6 hours at 20Β°C), measured by absorbance spectrophotometry (colorimetric assay, Ξ²-naphtol and Fast Blue B salt reaction product absorbance measurement, 540 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 50% | 100 | 9 | % | Incubation: 0.1M sodium phosphate, Assay: 0.1M sodium phosphate, 0.75 mg of Fast blue B salt | Incubation: 7.8, Assay: 7.8 | Incubation: 20Β°C, Assay: 20Β°C | 0.75 mg Acetyl-DL-phenylalanine Ξ²-naphthyl ester | N-Acetyl-DL-phenylalanine , Ξ²-Naphthol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Activity + Stability - Incubation | Activity after incubation (6 hours at 20Β°C), measured by absorbance spectrophotometry (colorimetric assay, Ξ²-naphtol and Fast Blue B salt reaction product absorbance measurement, 540 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 55% | 100 | 0 | % | Incubation: 0.1M sodium phosphate, Assay: 0.1M sodium phosphate, 0.75 mg of Fast blue B salt | Incubation: 7.8, Assay: 7.8 | Incubation: 20Β°C, Assay: 20Β°C | 0.75 mg Acetyl-DL-phenylalanine Ξ²-naphthyl ester | N-Acetyl-DL-phenylalanine , Ξ²-Naphthol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Activity + Stability - Incubation | Activity after incubation (6 hours at 20Β°C), measured by absorbance spectrophotometry (colorimetric assay, Ξ²-naphtol and Fast Blue B salt reaction product absorbance measurement, 540 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 60% | 100 | 0 | % | Incubation: 0.1M sodium phosphate, Assay: 0.1M sodium phosphate, 0.75 mg of Fast blue B salt | Incubation: 7.8, Assay: 7.8 | Incubation: 20Β°C, Assay: 20Β°C | 0.75 mg Acetyl-DL-phenylalanine Ξ²-naphthyl ester | N-Acetyl-DL-phenylalanine , Ξ²-Naphthol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Activity + Stability - Incubation | Activity after incubation (6 hours at 20Β°C), measured by absorbance spectrophotometry (colorimetric assay, Ξ²-naphtol and Fast Blue B salt reaction product absorbance measurement, 540 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 70% | 100 | 0 | % | Incubation: 0.1M sodium phosphate, Assay: 0.1M sodium phosphate, 0.75 mg of Fast blue B salt | Incubation: 7.8, Assay: 7.8 | Incubation: 20Β°C, Assay: 20Β°C | 0.75 mg Acetyl-DL-phenylalanine Ξ²-naphthyl ester | N-Acetyl-DL-phenylalanine , Ξ²-Naphthol | β | β | Non-incubated control (in %), assay in aqueous phase |
Visualization : Activity + Stability β Incubation
Linus Olofsson et al. (2006)
One bar per measurement. Colour = solvent, shade = solvent volume.
Hiroyasu Ogino et al. (2007)
β Stabilities and Conformational Transitions of Various Proteases in the Presence of an Organic Solvent
Biotechnology Progress
Β· doi:10.1021/bp060252p β
Β· Stability - Half-life
▼
Database ID
UniProt: P00766 β
Sequence Annotation
Inferred - from protein name
Protein Source
Commercially purchased
Experimental Data (1 measurement)
1 measurement
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Half-life | Half-life (in hours) at 30Β°C measured using absorbance spectrophotometry (free aromatic residues absorbance measurement, 280 nm) | Methanol | 25% | 1.26 | 0.15 | hours | Incubation: 10 mM sodium phosphate buffer, Assay: 50 mM borax-HCl | Incubation: ?, Assay: 8.5 | Incubation: 30Β°C, Assay: 30Β°C | 0.6% (w/v) Casein | free amino acids , Peptides | β | β | Classical aqueous control (in hours) |
Visualization : Stability β Half-life
Hiroyasu Ogino et al. (2007)
One bar per measurement. Colour = solvent, shade = solvent volume.
Lena Ostermeier et al. (2020)
β The multifaceted effects of DMSO and high hydrostatic pressure on the kinetic constants of hydrolysis reactions catalyzed by Ξ±-chymotrypsin
Physical Chemistry Chemical Physics
Β· doi:10.1039/d0cp03062g β
Β· Activity - Classical Activity - Michaelis-Menten
▼
Database ID
UniProt: P00766 β
Sequence Annotation
Inferred - from protein name
Protein Source
Commercially purchased
Experimental Data (12 measurements)
12 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | 1/turnover time (in 1/s), measured by fluorescence spectrophotometry (7-amino-4-methylcoumarin fluorescence measurement, excitation at 370 nm and absorbance at 460 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 5% | 0.075 | 0.05 | 1/s | 100 mM Tris-HCl buffer, 10 mM CaCl2 | 7.8 | 20Β°C | 20 Β΅M AlanineβAlanineβPhenylalanineβ7-amido-4-methylcoumarin | 7-amino-4-methylcoumarin , AlaβAlaβPhe | β | β | Classical aqueous control (in 1/s) |
| Activity - Classical | 1/turnover time (in 1/s), measured by fluorescence spectrophotometry (7-amino-4-methylcoumarin fluorescence measurement, excitation at 370 nm and absorbance at 460 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 10% | 0.075 | 0.035 | 1/s | 100 mM Tris-HCl buffer, 10 mM CaCl2 | 7.8 | 20Β°C | 20 Β΅M AlanineβAlanineβPhenylalanineβ7-amido-4-methylcoumarin | 7-amino-4-methylcoumarin , AlaβAlaβPhe | β | β | Classical aqueous control (in 1/s) |
| Activity - Classical | 1/turnover time (in 1/s), measured by fluorescence spectrophotometry (7-amino-4-methylcoumarin fluorescence measurement, excitation at 370 nm and absorbance at 460 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 20% | 0.075 | 0.014 | 1/s | 100 mM Tris-HCl buffer, 10 mM CaCl2 | 7.8 | 20Β°C | 20 Β΅M AlanineβAlanineβPhenylalanineβ7-amido-4-methylcoumarin | 7-amino-4-methylcoumarin , AlaβAlaβPhe | β | β | Classical aqueous control (in 1/s) |
| Activity - Michaelis-Menten | Km (Β΅M), measured by fluorescence spectrophotometry (7-amino-4-methylcoumarin fluorescence measurement, excitation at 370 nm and absorbance at 460 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 5% | 316.0 | 397.0 | Β΅M | 100 mM Tris-HCl buffer, 10 mM CaCl2 | 7.8 | 20Β°C | AlanineβAlanineβPhenylalanineβ7-amido-4-methylcoumarin | 7-amino-4-methylcoumarin , AlaβAlaβPhe | β | β | Classical aqueous control (in microM) |
| Activity - Michaelis-Menten | Km (Β΅M), measured by fluorescence spectrophotometry (7-amino-4-methylcoumarin fluorescence measurement, excitation at 370 nm and absorbance at 460 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 10% | 316.0 | 481.0 | Β΅M | 100 mM Tris-HCl buffer, 10 mM CaCl2 | 7.8 | 20Β°C | AlanineβAlanineβPhenylalanineβ7-amido-4-methylcoumarin | 7-amino-4-methylcoumarin , AlaβAlaβPhe | β | β | Classical aqueous control (in microM) |
| Activity - Michaelis-Menten | Km (Β΅M), measured by fluorescence spectrophotometry (7-amino-4-methylcoumarin fluorescence measurement, excitation at 370 nm and absorbance at 460 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 20% | 316.0 | 328.0 | Β΅M | 100 mM Tris-HCl buffer, 10 mM CaCl2 | 7.8 | 20Β°C | AlanineβAlanineβPhenylalanineβ7-amido-4-methylcoumarin | 7-amino-4-methylcoumarin , AlaβAlaβPhe | β | β | Classical aqueous control (in microM) |
| Activity - Michaelis-Menten | kcat (1/s), measured by fluorescence spectrophotometry (7-amino-4-methylcoumarin fluorescence measurement, excitation at 370 nm and absorbance at 460 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 5% | 1.16 | 1.0 | 1/s | 100 mM Tris-HCl buffer, 10 mM CaCl2 | 7.8 | 20Β°C | AlanineβAlanineβPhenylalanineβ7-amido-4-methylcoumarin | 7-amino-4-methylcoumarin , AlaβAlaβPhe | β | β | Classical aqueous control (in 1/s) |
| Activity - Michaelis-Menten | kcat (1/s), measured by fluorescence spectrophotometry (7-amino-4-methylcoumarin fluorescence measurement, excitation at 370 nm and absorbance at 460 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 10% | 1.16 | 0.88 | 1/s | 100 mM Tris-HCl buffer, 10 mM CaCl2 | 7.8 | 20Β°C | AlanineβAlanineβPhenylalanineβ7-amido-4-methylcoumarin | 7-amino-4-methylcoumarin , AlaβAlaβPhe | β | β | Classical aqueous control (in 1/s) |
| Activity - Michaelis-Menten | kcat (1/s), measured by fluorescence spectrophotometry (7-amino-4-methylcoumarin fluorescence measurement, excitation at 370 nm and absorbance at 460 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 20% | 1.16 | 0.25 | 1/s | 100 mM Tris-HCl buffer, 10 mM CaCl2 | 7.8 | 20Β°C | AlanineβAlanineβPhenylalanineβ7-amido-4-methylcoumarin | 7-amino-4-methylcoumarin , AlaβAlaβPhe | β | β | Classical aqueous control (in 1/s) |
| Activity - Michaelis-Menten | kcat/Km (10^3*s^-1*Β΅M^-1), measured by fluorescence spectrophotometry (7-amino-4-methylcoumarin fluorescence measurement, excitation at 370 nm and absorbance at 460 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 5% | 3.67 | 2.52 | 10^3 * s^-1 * Β΅M^-1 | 100 mM Tris-HCl buffer, 10 mM CaCl2 | 7.8 | 20Β°C | AlanineβAlanineβPhenylalanineβ7-amido-4-methylcoumarin | 7-amino-4-methylcoumarin , AlaβAlaβPhe | β | β | Classical aqueous control (in 10^3 * s^-1 * microM^-1) |
| Activity - Michaelis-Menten | kcat/Km (10^3*s^-1*Β΅M^-1), measured by fluorescence spectrophotometry (7-amino-4-methylcoumarin fluorescence measurement, excitation at 370 nm and absorbance at 460 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 10% | 3.67 | 1.83 | 10^3 * s^-1 * Β΅M^-1 | 100 mM Tris-HCl buffer, 10 mM CaCl2 | 7.8 | 20Β°C | AlanineβAlanineβPhenylalanineβ7-amido-4-methylcoumarin | 7-amino-4-methylcoumarin , AlaβAlaβPhe | β | β | Classical aqueous control (in 10^3 * s^-1 * microM^-1) |
| Activity - Michaelis-Menten | kcat/Km (10^3*s^-1*Β΅M^-1), measured by fluorescence spectrophotometry (7-amino-4-methylcoumarin fluorescence measurement, excitation at 370 nm and absorbance at 460 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 20% | 3.67 | 0.76 | 10^3 * s^-1 * Β΅M^-1 | 100 mM Tris-HCl buffer, 10 mM CaCl2 | 7.8 | 20Β°C | AlanineβAlanineβPhenylalanineβ7-amido-4-methylcoumarin | 7-amino-4-methylcoumarin , AlaβAlaβPhe | β | β | Classical aqueous control (in 10^3 * s^-1 * microM^-1) |
Visualization : Activity β Classical
Lena Ostermeier et al. (2020)
One bar per measurement. Colour = solvent, shade = solvent volume.
Visualization : Activity β Michaelis-Menten
Lena Ostermeier et al. (2020)
One bar per measurement. Colour = solvent, shade = solvent volume.