NADP-dependent malic enzyme from Sulfolobus solfataricus

Enzyme Description

Species
Sulfolobus solfataricus β†—
Extremophile
Yes "extreme thermoacidophilic" organism
EC Number
1.1.1.40 β†—

Sequence

Length: 427 amino acids
MVDAVELSRKYEGKIEIYPKVPITSYNDFALIYTPGVAEVSKAIYKDPDKSFELTSRWNTIAVVTDGTRVLGLGNIGPTAAMPVMEGKSLLFKYLGGVDAIPLPINVKSADEFTNVVKALETSFGGINLEDIESPKCFYILEKLQSMLNIPVWHDDQQGTAAAILAGLINAMILTNKDPKESKVVFYGAGASNIAAARILAKYGFKYENIVMIDSKGPLYADREDVDHLMLHHKWKYELAIKTNKWEAKEIKDAFSGADAVVAASTPGPNVIKKEWISLMNKEAIVFALANPVPEIWPQDAKEAGAKIVATGRSDFPNQVNNSLIFPAIFRGVLDSRAKAITDEMTIAASEALAKYARDRGINENYIIPRMDEWEAFYEEAAAVASKAVELGLARVKKSREEFREIAKERIIRARKIMNLVISTWSP
Annamaria Guagliardi et al. (1989) β€” Stability and activity of a thermostable malic enzyme in denaturants and water-miscible organic solvents
European Journal of Biochemistry  Β· doi:10.1111/j.1432-1033.1989.tb14891.x β†—  Β· Activity - Classical Stability - Incubation
71 measurements ▼
Database ID
UniProt: A0A7S9IJ09 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Purified, archaeon Sulfolobus solfataricus

Experimental Data (71 measurements)

71 measurements β€” page 1 of 4
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Methanol 50% 100.0 138.6 % 20 mM Tris/HCl, 50 mM ammonium sulfate, 0.1 MM MnCl2 8 60 Β°C 1 mM L-Malic acid Carbon Dioxide (CO2) , Pyruvate 0.05 mM NADP+ β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Methanol 5% 100.0 105.5 % 20 mM Tris/HCl, 50 mM ammonium sulfate, 0.1 MM MnCl2 8 25 Β°C 1 mM L-Malic acid Carbon Dioxide (CO2) , Pyruvate 0.05 mM NADP+ β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Methanol 10% 100.0 126.8 % 20 mM Tris/HCl, 50 mM ammonium sulfate, 0.1 MM MnCl2 8 25 Β°C 1 mM L-Malic acid Carbon Dioxide (CO2) , Pyruvate 0.05 mM NADP+ β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Methanol 30% 100.0 141.6 % 20 mM Tris/HCl, 50 mM ammonium sulfate, 0.1 MM MnCl2 8 25 Β°C 1 mM L-Malic acid Carbon Dioxide (CO2) , Pyruvate 0.05 mM NADP+ β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Methanol 40% 100.0 159.7 % 20 mM Tris/HCl, 50 mM ammonium sulfate, 0.1 MM MnCl2 8 25 Β°C 1 mM L-Malic acid Carbon Dioxide (CO2) , Pyruvate 0.05 mM NADP+ β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Methanol 50% 100.0 199.4 % 20 mM Tris/HCl, 50 mM ammonium sulfate, 0.1 MM MnCl2 8 25 Β°C 1 mM L-Malic acid Carbon Dioxide (CO2) , Pyruvate 0.05 mM NADP+ β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Methanol 60% 100.0 209.3 % 20 mM Tris/HCl, 50 mM ammonium sulfate, 0.1 MM MnCl2 8 25 Β°C 1 mM L-Malic acid Carbon Dioxide (CO2) , Pyruvate 0.05 mM NADP+ β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Methanol 70% 100.0 203.6 % 20 mM Tris/HCl, 50 mM ammonium sulfate, 0.1 MM MnCl2 8 25 Β°C 1 mM L-Malic acid Carbon Dioxide (CO2) , Pyruvate 0.05 mM NADP+ β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Ethanol 10% 100.0 122.7 % 20 mM Tris/HCl, 50 mM ammonium sulfate, 0.1 MM MnCl2 8 25 Β°C 1 mM L-Malic acid Carbon Dioxide (CO2) , Pyruvate 0.05 mM NADP+ β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Ethanol 30% 100.0 135.6 % 20 mM Tris/HCl, 50 mM ammonium sulfate, 0.1 MM MnCl2 8 25 Β°C 1 mM L-Malic acid Carbon Dioxide (CO2) , Pyruvate 0.05 mM NADP+ β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Ethanol 40% 100.0 153.4 % 20 mM Tris/HCl, 50 mM ammonium sulfate, 0.1 MM MnCl2 8 25 Β°C 1 mM L-Malic acid Carbon Dioxide (CO2) , Pyruvate 0.05 mM NADP+ β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Ethanol 50% 100.0 172.3 % 20 mM Tris/HCl, 50 mM ammonium sulfate, 0.1 MM MnCl2 8 25 Β°C 1 mM L-Malic acid Carbon Dioxide (CO2) , Pyruvate 0.05 mM NADP+ β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Ethanol 60% 100.0 157.3 % 20 mM Tris/HCl, 50 mM ammonium sulfate, 0.1 MM MnCl2 8 25 Β°C 1 mM L-Malic acid Carbon Dioxide (CO2) , Pyruvate 0.05 mM NADP+ β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Ethanol 70% 100.0 142.2 % 20 mM Tris/HCl, 50 mM ammonium sulfate, 0.1 MM MnCl2 8 25 Β°C 1 mM L-Malic acid Carbon Dioxide (CO2) , Pyruvate 0.05 mM NADP+ β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Acetone 10% 100.0 120.0 % 20 mM Tris/HCl, 50 mM ammonium sulfate, 0.1 MM MnCl2 8 25 Β°C 1 mM L-Malic acid Carbon Dioxide (CO2) , Pyruvate 0.05 mM NADP+ β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Acetone 30% 100.0 146.0 % 20 mM Tris/HCl, 50 mM ammonium sulfate, 0.1 MM MnCl2 8 25 Β°C 1 mM L-Malic acid Carbon Dioxide (CO2) , Pyruvate 0.05 mM NADP+ β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Acetone 50% 100.0 133.4 % 20 mM Tris/HCl, 50 mM ammonium sulfate, 0.1 MM MnCl2 8 25 Β°C 1 mM L-Malic acid Carbon Dioxide (CO2) , Pyruvate 0.05 mM NADP+ β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Methanol 30% 100.0 126.0 % 20 mM Tris/HCl, 50 mM ammonium sulfate, 0.1 MM MnCl2 8 60 Β°C 1 mM L-Malic acid Carbon Dioxide (CO2) , Pyruvate 0.05 mM NADP+ β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Methanol 10% 100.0 113.7 % 20 mM Tris/HCl, 50 mM ammonium sulfate, 0.1 MM MnCl2 8 60 Β°C 1 mM L-Malic acid Carbon Dioxide (CO2) , Pyruvate 0.05 mM NADP+ β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Methanol 50% 100.0 159.5 % 20 mM Tris/HCl, 50 mM ammonium sulfate, 0.1 MM MnCl2 8 45 Β°C 1 mM L-Malic acid Carbon Dioxide (CO2) , Pyruvate 0.05 mM NADP+ β€” Classical aqueous control (in %)
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Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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