Alcohol dehydrogenase (gene adh2) from Haloferax volcanii
Enzyme Description
Sequence
MKSAVYRGPGDVRIEEKPEPELQQPTDAVIRVTHTAICGSDLWFYRGHEEHEEGSGVGHEPMGIVEEVGEDVTSVQPGDRVLVPFAYSCGECEFCRKGIHTACVDGGFFGPHGDETGGAQAEKVRVLHADGTVVRVPERYADDEETLKSLLPLTDVMSTGHHAAVLADLEAGDTAVVIGDGAVGLCGVLAAKRIGAERIIAVGHHEDRLEVARELGATDTVSSRGQEAVEEVLELTHGGANHVLECVGAASALETAAQVARPGGSIGAVGVPHVESADFLQPIFYKNLSFSAGGAPVRNYIEELMDDVLQGTLDPSPILTKTVDLDGVPEGYQAMDDREAIKVMVKVDE
Leanne M. Timpson et al. (2012)
β A comparison of two novel alcohol dehydrogenase enzymes (ADH1 and ADH2) from the extreme halophile Haloferax volcanii
Applied Microbiology and Biotechnology
Β· doi:10.1007/s00253-012-4074-4 β
Β· Stability - Incubation
▼
Database ID
UniProt: D4GP73 β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host archaeon Haloferax volcanii
Experimental Data (14 measurements)
14 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) | Dimethyl Sulfoxide (DMSO) | 10% | 100 | 85 | % | Incubation: 100 mM Tris-HCl, 2M KCl, Assay: 50 mM glycineβKOH, 4M KCl | Incubation: 8, Assay: 10 | Incubation: 4Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NAD+ | β | Non-incubated control (in %), control and assay in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) | Dimethyl Sulfoxide (DMSO) | 20% | 100 | 85 | % | Incubation: 100 mM Tris-HCl, 2M KCl, Assay: 50 mM glycineβKOH, 4M KCl | Incubation: 8, Assay: 10 | Incubation: 4Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NAD+ | β | Non-incubated control (in %), control and assay in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) | Acetonitrile | 10% | 100 | 87 | % | Incubation: 100 mM Tris-HCl, 2M KCl, Assay: 50 mM glycineβKOH, 4M KCl | Incubation: 8, Assay: 10 | Incubation: 4Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NAD+ | β | Non-incubated control (in %), control and assay in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) | Acetonitrile | 20% | 100 | 18 | % | Incubation: 100 mM Tris-HCl, 2M KCl, Assay: 50 mM glycineβKOH, 4M KCl | Incubation: 8, Assay: 10 | Incubation: 4Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NAD+ | β | Non-incubated control (in %), control and assay in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) | Tetrahydrofuran (THF) | 10% | 100 | 3 | % | Incubation: 100 mM Tris-HCl, 2M KCl, Assay: 50 mM glycineβKOH, 4M KCl | Incubation: 8, Assay: 10 | Incubation: 4Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NAD+ | β | Non-incubated control (in %), control and assay in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (72 hours at 4Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) | Dimethyl Sulfoxide (DMSO) | 5% | 100 | 74 | % | Incubation: 100 mM Tris-HCl, 2M KCl, Assay: 50 mM glycineβKOH, 4M KCl | Incubation: 8, Assay: 10 | Incubation: 4Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NAD+ | β | Non-incubated control (in %), control and assay in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (72 hours at 4Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) | Dimethyl Sulfoxide (DMSO) | 10% | 100 | 69 | % | Incubation: 100 mM Tris-HCl, 2M KCl, Assay: 50 mM glycineβKOH, 4M KCl | Incubation: 8, Assay: 10 | Incubation: 4Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NAD+ | β | Non-incubated control (in %), control and assay in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (72 hours at 4Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) | Dimethyl Sulfoxide (DMSO) | 30% | 100 | 47 | % | Incubation: 100 mM Tris-HCl, 2M KCl, Assay: 50 mM glycineβKOH, 4M KCl | Incubation: 8, Assay: 10 | Incubation: 4Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NAD+ | β | Non-incubated control (in %), control and assay in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (72 hours at 4Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) | Acetonitrile | 5% | 100 | 65 | % | Incubation: 100 mM Tris-HCl, 2M KCl, Assay: 50 mM glycineβKOH, 4M KCl | Incubation: 8, Assay: 10 | Incubation: 4Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NAD+ | β | Non-incubated control (in %), control and assay in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (72 hours at 4Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) | Acetonitrile | 10% | 100 | 63 | % | Incubation: 100 mM Tris-HCl, 2M KCl, Assay: 50 mM glycineβKOH, 4M KCl | Incubation: 8, Assay: 10 | Incubation: 4Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NAD+ | β | Non-incubated control (in %), control and assay in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (72 hours at 4Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) | Acetonitrile | 30% | 100 | 0 | % | Incubation: 100 mM Tris-HCl, 2M KCl, Assay: 50 mM glycineβKOH, 4M KCl | Incubation: 8, Assay: 10 | Incubation: 4Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NAD+ | β | Non-incubated control (in %), control and assay in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (72 hours at 4Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) | Methanol | 5% | 100 | 70 | % | Incubation: 100 mM Tris-HCl, 2M KCl, Assay: 50 mM glycineβKOH, 4M KCl | Incubation: 8, Assay: 10 | Incubation: 4Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NAD+ | β | Non-incubated control (in %), control and assay in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (72 hours at 4Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) | Methanol | 10% | 100 | 67 | % | Incubation: 100 mM Tris-HCl, 2M KCl, Assay: 50 mM glycineβKOH, 4M KCl | Incubation: 8, Assay: 10 | Incubation: 4Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NAD+ | β | Non-incubated control (in %), control and assay in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (72 hours at 4Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) | Methanol | 30% | 100 | 39 | % | Incubation: 100 mM Tris-HCl, 2M KCl, Assay: 50 mM glycineβKOH, 4M KCl | Incubation: 8, Assay: 10 | Incubation: 4Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NAD+ | β | Non-incubated control (in %), control and assay in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control |
Visualization : Stability β Incubation
Leanne M. Timpson et al. (2012)
One bar per measurement. Colour = solvent, shade = solvent volume.
Diya Alsafadi et al. (2012)
β Effect of organic solvents on the activity and stability of halophilic alcohol dehydrogenase (ADH2) from Haloferax volcanii
▼
Database ID
UniProt: D4GP73 β
Sequence Annotation
Inferred - from protein name
Protein Source
Recombinant, host archaeon Haloferax volcanii
Experimental Data (105 measurements)
105 measurements β page 5 of 6
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (72 hours at 50Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Acetonitrile | 10% | 52.0 | 34.0 | % | Incubation: 100 mM Tris-HCl, 3M KCl, Assay: 50 mM glycineβKOH, 4M KCl | Incubation: 8, Assay: 10 | Incubation: 50Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NADP+ | β | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (0 hour at 50Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Acetonitrile | 30% | 100.0 | 5.0 | % | Incubation: 100 mM Tris-HCl, 3M KCl, Assay: 50 mM glycineβKOH, 4M KCl | Incubation: 8, Assay: 10 | Incubation: 50Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NADP+ | β | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (24 hours at 50Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Acetonitrile | 30% | 77.0 | 0.0 | % | Incubation: 100 mM Tris-HCl, 3M KCl, Assay: 50 mM glycineβKOH, 4M KCl | Incubation: 8, Assay: 10 | Incubation: 50Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NADP+ | β | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (72 hours at 50Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Acetonitrile | 30% | 52.0 | 0.0 | % | Incubation: 100 mM Tris-HCl, 3M KCl, Assay: 50 mM glycineβKOH, 4M KCl | Incubation: 8, Assay: 10 | Incubation: 50Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NADP+ | β | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (0 hour at 50Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Methanol | 5% | 100.0 | 100.0 | % | Incubation: 100 mM Tris-HCl, 3M KCl, Assay: 50 mM glycineβKOH, 4M KCl | Incubation: 8, Assay: 10 | Incubation: 50Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NADP+ | β | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (24 hours at 50Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Methanol | 5% | 77.0 | 69.0 | % | Incubation: 100 mM Tris-HCl, 3M KCl, Assay: 50 mM glycineβKOH, 4M KCl | Incubation: 8, Assay: 10 | Incubation: 50Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NADP+ | β | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (72 hours at 50Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Methanol | 5% | 52.0 | 69.0 | % | Incubation: 100 mM Tris-HCl, 3M KCl, Assay: 50 mM glycineβKOH, 4M KCl | Incubation: 8, Assay: 10 | Incubation: 50Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NADP+ | β | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (0 hour at 50Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Methanol | 10% | 100.0 | 100.0 | % | Incubation: 100 mM Tris-HCl, 3M KCl, Assay: 50 mM glycineβKOH, 4M KCl | Incubation: 8, Assay: 10 | Incubation: 50Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NADP+ | β | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (24 hours at 50Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Methanol | 10% | 77.0 | 73.0 | % | Incubation: 100 mM Tris-HCl, 3M KCl, Assay: 50 mM glycineβKOH, 4M KCl | Incubation: 8, Assay: 10 | Incubation: 50Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NADP+ | β | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (72 hours at 50Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Methanol | 10% | 52.0 | 67.0 | % | Incubation: 100 mM Tris-HCl, 3M KCl, Assay: 50 mM glycineβKOH, 4M KCl | Incubation: 8, Assay: 10 | Incubation: 50Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NADP+ | β | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (0 hour at 50Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Methanol | 30% | 100.0 | 53.0 | % | Incubation: 100 mM Tris-HCl, 3M KCl, Assay: 50 mM glycineβKOH, 4M KCl | Incubation: 8, Assay: 10 | Incubation: 50Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NADP+ | β | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (24 hours at 50Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Methanol | 30% | 77.0 | 0.0 | % | Incubation: 100 mM Tris-HCl, 3M KCl, Assay: 50 mM glycineβKOH, 4M KCl | Incubation: 8, Assay: 10 | Incubation: 50Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NADP+ | β | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (72 hours at 50Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Methanol | 30% | 52.0 | 0.0 | % | Incubation: 100 mM Tris-HCl, 3M KCl, Assay: 50 mM glycineβKOH, 4M KCl | Incubation: 8, Assay: 10 | Incubation: 50Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NADP+ | β | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (20 days at 5Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 5% | 0.4 | 81.1 | % | Incubation: 50 mM citric acid-K2HPO4, 2M KCl, Assay: 50 mM citric acid-K2HPO4, 2M KCl | Incubation: 6, Assay: 10 | Incubation: 5Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NADP+ | β | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (20 days at 5Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 5% | 69.0 | 84.0 | % | Incubation: 50 mM Tris-HCl, 2M KCl, Assay: 50 mM Tris-HCl, 2M KCl | Incubation: 8, Assay: 10 | Incubation: 5Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NADP+ | β | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (20 days at 5Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 5% | 56.0 | 100.0 | % | Incubation: 50 mM glycine-KaOH, 2M KCl, Assay: 50 mM glycine-KaOH, 2M KCl | Incubation: 10, Assay: 10 | Incubation: 5Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NADP+ | β | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (20 days at 5Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 10% | 0.4 | 63.1 | % | Incubation: 50 mM citric acid-K2HPO4, 2M KCl, Assay: 50 mM citric acid-K2HPO4, 2M KCl | Incubation: 6, Assay: 10 | Incubation: 5Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NADP+ | β | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (20 days at 5Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 10% | 69.0 | 95.0 | % | Incubation: 50 mM Tris-HCl, 2M KCl, Assay: 50 mM Tris-HCl, 2M KCl | Incubation: 8, Assay: 10 | Incubation: 5Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NADP+ | β | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (20 days at 5Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 10% | 56.0 | 105.0 | % | Incubation: 50 mM glycine-KaOH, 2M KCl, Assay: 50 mM glycine-KaOH, 2M KCl | Incubation: 10, Assay: 10 | Incubation: 5Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NADP+ | β | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (20 days at 5Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Methanol | 5% | 0.4 | 1.0 | % | Incubation: 50 mM citric acid-K2HPO4, 2M KCl, Assay: 50 mM citric acid-K2HPO4, 2M KCl | Incubation: 6, Assay: 10 | Incubation: 5Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NADP+ | β | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether activity was measured with or without organic solvent |
Visualization : Activity β Classical
Diya Alsafadi et al. (2012)
One bar per measurement. Colour = solvent, shade = solvent volume.
Visualization : Stability β Incubation
Diya Alsafadi et al. (2012)
One bar per measurement. Colour = solvent, shade = solvent volume.
Ann-Kathrin Liliensiek et al. (2013)
β Heterologous Overexpression, Purification and Characterisation of an Alcohol Dehydrogenase (ADH2) from Halobacterium sp. NRC-1
Molecular Biotechnology
Β· doi:10.1007/s12033-013-9666-4 β
Β· Stability - Incubation
▼
Database ID
UniProt: D4GP73 β
Sequence Annotation
Inferred - from protein name
Protein Source
Recombinant, host archaeon Haloferax volcanii (H1209-H1325)
Experimental Data (12 measurements)
12 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NAD(P)H absorbance measurment) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 5% | 100 | 99 | % | Incubation: 100 mM Tris-HCl, 2M NaCl, Assay: 50 mM glycine-KOH, 4M KCl | Incubation: 7.5, Assay: 10 | Incubation: 4Β°C, Assay: 50Β°C | 100 mM 1-Propanol | Propanal | 1 mM NAD(P)+ | β | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NAD(P)H absorbance measurment) in aqueous phase | Acetonitrile | 5% | 100 | 79 | % | Incubation: 100 mM Tris-HCl, 2M NaCl, Assay: 50 mM glycine-KOH, 4M KCl | Incubation: 7.5, Assay: 10 | Incubation: 4Β°C, Assay: 50Β°C | 100 mM 1-Propanol | Propanal | 1 mM NAD(P)+ | β | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NAD(P)H absorbance measurment) in aqueous phase | Methanol | 5% | 100 | 83 | % | Incubation: 100 mM Tris-HCl, 2M NaCl, Assay: 50 mM glycine-KOH, 4M KCl | Incubation: 7.5, Assay: 10 | Incubation: 4Β°C, Assay: 50Β°C | 100 mM 1-Propanol | Propanal | 1 mM NAD(P)+ | β | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NAD(P)H absorbance measurment) in aqueous phase | Tetrahydrofuran (THF) | 5% | 100 | 32 | % | Incubation: 100 mM Tris-HCl, 2M NaCl, Assay: 50 mM glycine-KOH, 4M KCl | Incubation: 7.5, Assay: 10 | Incubation: 4Β°C, Assay: 50Β°C | 100 mM 1-Propanol | Propanal | 1 mM NAD(P)+ | β | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NAD(P)H absorbance measurment) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 10% | 100 | 74 | % | Incubation: 100 mM Tris-HCl, 2M NaCl, Assay: 50 mM glycine-KOH, 4M KCl | Incubation: 7.5, Assay: 10 | Incubation: 4Β°C, Assay: 50Β°C | 100 mM 1-Propanol | Propanal | 1 mM NAD(P)+ | β | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NAD(P)H absorbance measurment) in aqueous phase | Acetonitrile | 10% | 100 | 74 | % | Incubation: 100 mM Tris-HCl, 2M NaCl, Assay: 50 mM glycine-KOH, 4M KCl | Incubation: 7.5, Assay: 10 | Incubation: 4Β°C, Assay: 50Β°C | 100 mM 1-Propanol | Propanal | 1 mM NAD(P)+ | β | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NAD(P)H absorbance measurment) in aqueous phase | Methanol | 10% | 100 | 77 | % | Incubation: 100 mM Tris-HCl, 2M NaCl, Assay: 50 mM glycine-KOH, 4M KCl | Incubation: 7.5, Assay: 10 | Incubation: 4Β°C, Assay: 50Β°C | 100 mM 1-Propanol | Propanal | 1 mM NAD(P)+ | β | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NAD(P)H absorbance measurment) in aqueous phase | Tetrahydrofuran (THF) | 10% | 100 | 12 | % | Incubation: 100 mM Tris-HCl, 2M NaCl, Assay: 50 mM glycine-KOH, 4M KCl | Incubation: 7.5, Assay: 10 | Incubation: 4Β°C, Assay: 50Β°C | 100 mM 1-Propanol | Propanal | 1 mM NAD(P)+ | β | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NAD(P)H absorbance measurment) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 30% | 100 | 54 | % | Incubation: 100 mM Tris-HCl, 2M NaCl, Assay: 50 mM glycine-KOH, 4M KCl | Incubation: 7.5, Assay: 10 | Incubation: 4Β°C, Assay: 50Β°C | 100 mM 1-Propanol | Propanal | 1 mM NAD(P)+ | β | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NAD(P)H absorbance measurment) in aqueous phase | Acetonitrile | 30% | 100 | 0 | % | Incubation: 100 mM Tris-HCl, 2M NaCl, Assay: 50 mM glycine-KOH, 4M KCl | Incubation: 7.5, Assay: 10 | Incubation: 4Β°C, Assay: 50Β°C | 100 mM 1-Propanol | Propanal | 1 mM NAD(P)+ | β | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NAD(P)H absorbance measurment) in aqueous phase | Methanol | 30% | 100 | 43 | % | Incubation: 100 mM Tris-HCl, 2M NaCl, Assay: 50 mM glycine-KOH, 4M KCl | Incubation: 7.5, Assay: 10 | Incubation: 4Β°C, Assay: 50Β°C | 100 mM 1-Propanol | Propanal | 1 mM NAD(P)+ | β | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NAD(P)H absorbance measurment) in aqueous phase | Tetrahydrofuran (THF) | 30% | 100 | 0 | % | Incubation: 100 mM Tris-HCl, 2M NaCl, Assay: 50 mM glycine-KOH, 4M KCl | Incubation: 7.5, Assay: 10 | Incubation: 4Β°C, Assay: 50Β°C | 100 mM 1-Propanol | Propanal | 1 mM NAD(P)+ | β | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
Visualization : Stability β Incubation
Ann-Kathrin Liliensiek et al. (2013)
One bar per measurement. Colour = solvent, shade = solvent volume.