Alcohol dehydrogenase (gene adh2) from Haloferax volcanii

Enzyme Description

Species
Haloferax volcanii β†—
Extremophile
Yes "halophilic" organism
EC Number
1.1.1.71 β†—

Sequence

Length: 349 amino acids
MKSAVYRGPGDVRIEEKPEPELQQPTDAVIRVTHTAICGSDLWFYRGHEEHEEGSGVGHEPMGIVEEVGEDVTSVQPGDRVLVPFAYSCGECEFCRKGIHTACVDGGFFGPHGDETGGAQAEKVRVLHADGTVVRVPERYADDEETLKSLLPLTDVMSTGHHAAVLADLEAGDTAVVIGDGAVGLCGVLAAKRIGAERIIAVGHHEDRLEVARELGATDTVSSRGQEAVEEVLELTHGGANHVLECVGAASALETAAQVARPGGSIGAVGVPHVESADFLQPIFYKNLSFSAGGAPVRNYIEELMDDVLQGTLDPSPILTKTVDLDGVPEGYQAMDDREAIKVMVKVDE
Leanne M. Timpson et al. (2012) β€” A comparison of two novel alcohol dehydrogenase enzymes (ADH1 and ADH2) from the extreme halophile Haloferax volcanii
Applied Microbiology and Biotechnology  Β· doi:10.1007/s00253-012-4074-4 β†—  Β· Stability - Incubation
14 measurements ▼
Database ID
UniProt: D4GP73 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host archaeon Haloferax volcanii

Experimental Data (14 measurements)

14 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) Dimethyl Sulfoxide (DMSO) 10% 100 85 % Incubation: 100 mM Tris-HCl, 2M KCl, Assay: 50 mM glycine–KOH, 4M KCl Incubation: 8, Assay: 10 Incubation: 4Β°C, Assay: 50Β°C 100 mM Ethanol Acetaldehyde 1 mM NAD+ β€” Non-incubated control (in %), control and assay in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) Dimethyl Sulfoxide (DMSO) 20% 100 85 % Incubation: 100 mM Tris-HCl, 2M KCl, Assay: 50 mM glycine–KOH, 4M KCl Incubation: 8, Assay: 10 Incubation: 4Β°C, Assay: 50Β°C 100 mM Ethanol Acetaldehyde 1 mM NAD+ β€” Non-incubated control (in %), control and assay in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) Acetonitrile 10% 100 87 % Incubation: 100 mM Tris-HCl, 2M KCl, Assay: 50 mM glycine–KOH, 4M KCl Incubation: 8, Assay: 10 Incubation: 4Β°C, Assay: 50Β°C 100 mM Ethanol Acetaldehyde 1 mM NAD+ β€” Non-incubated control (in %), control and assay in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) Acetonitrile 20% 100 18 % Incubation: 100 mM Tris-HCl, 2M KCl, Assay: 50 mM glycine–KOH, 4M KCl Incubation: 8, Assay: 10 Incubation: 4Β°C, Assay: 50Β°C 100 mM Ethanol Acetaldehyde 1 mM NAD+ β€” Non-incubated control (in %), control and assay in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) Tetrahydrofuran (THF) 10% 100 3 % Incubation: 100 mM Tris-HCl, 2M KCl, Assay: 50 mM glycine–KOH, 4M KCl Incubation: 8, Assay: 10 Incubation: 4Β°C, Assay: 50Β°C 100 mM Ethanol Acetaldehyde 1 mM NAD+ β€” Non-incubated control (in %), control and assay in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (72 hours at 4Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) Dimethyl Sulfoxide (DMSO) 5% 100 74 % Incubation: 100 mM Tris-HCl, 2M KCl, Assay: 50 mM glycine–KOH, 4M KCl Incubation: 8, Assay: 10 Incubation: 4Β°C, Assay: 50Β°C 100 mM Ethanol Acetaldehyde 1 mM NAD+ β€” Non-incubated control (in %), control and assay in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (72 hours at 4Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) Dimethyl Sulfoxide (DMSO) 10% 100 69 % Incubation: 100 mM Tris-HCl, 2M KCl, Assay: 50 mM glycine–KOH, 4M KCl Incubation: 8, Assay: 10 Incubation: 4Β°C, Assay: 50Β°C 100 mM Ethanol Acetaldehyde 1 mM NAD+ β€” Non-incubated control (in %), control and assay in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (72 hours at 4Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) Dimethyl Sulfoxide (DMSO) 30% 100 47 % Incubation: 100 mM Tris-HCl, 2M KCl, Assay: 50 mM glycine–KOH, 4M KCl Incubation: 8, Assay: 10 Incubation: 4Β°C, Assay: 50Β°C 100 mM Ethanol Acetaldehyde 1 mM NAD+ β€” Non-incubated control (in %), control and assay in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (72 hours at 4Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) Acetonitrile 5% 100 65 % Incubation: 100 mM Tris-HCl, 2M KCl, Assay: 50 mM glycine–KOH, 4M KCl Incubation: 8, Assay: 10 Incubation: 4Β°C, Assay: 50Β°C 100 mM Ethanol Acetaldehyde 1 mM NAD+ β€” Non-incubated control (in %), control and assay in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (72 hours at 4Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) Acetonitrile 10% 100 63 % Incubation: 100 mM Tris-HCl, 2M KCl, Assay: 50 mM glycine–KOH, 4M KCl Incubation: 8, Assay: 10 Incubation: 4Β°C, Assay: 50Β°C 100 mM Ethanol Acetaldehyde 1 mM NAD+ β€” Non-incubated control (in %), control and assay in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (72 hours at 4Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) Acetonitrile 30% 100 0 % Incubation: 100 mM Tris-HCl, 2M KCl, Assay: 50 mM glycine–KOH, 4M KCl Incubation: 8, Assay: 10 Incubation: 4Β°C, Assay: 50Β°C 100 mM Ethanol Acetaldehyde 1 mM NAD+ β€” Non-incubated control (in %), control and assay in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (72 hours at 4Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) Methanol 5% 100 70 % Incubation: 100 mM Tris-HCl, 2M KCl, Assay: 50 mM glycine–KOH, 4M KCl Incubation: 8, Assay: 10 Incubation: 4Β°C, Assay: 50Β°C 100 mM Ethanol Acetaldehyde 1 mM NAD+ β€” Non-incubated control (in %), control and assay in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (72 hours at 4Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) Methanol 10% 100 67 % Incubation: 100 mM Tris-HCl, 2M KCl, Assay: 50 mM glycine–KOH, 4M KCl Incubation: 8, Assay: 10 Incubation: 4Β°C, Assay: 50Β°C 100 mM Ethanol Acetaldehyde 1 mM NAD+ β€” Non-incubated control (in %), control and assay in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (72 hours at 4Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) Methanol 30% 100 39 % Incubation: 100 mM Tris-HCl, 2M KCl, Assay: 50 mM glycine–KOH, 4M KCl Incubation: 8, Assay: 10 Incubation: 4Β°C, Assay: 50Β°C 100 mM Ethanol Acetaldehyde 1 mM NAD+ β€” Non-incubated control (in %), control and assay in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control

Visualization : Stability β€” Incubation

Leanne M. Timpson et al. (2012)

One bar per measurement. Colour = solvent, shade = solvent volume.

Diya Alsafadi et al. (2012) β€” Effect of organic solvents on the activity and stability of halophilic alcohol dehydrogenase (ADH2) from Haloferax volcanii
Extremophiles  Β· doi:10.1007/s00792-012-0498-0 β†—  Β· Activity - Classical Stability - Incubation
105 measurements ▼
Ann-Kathrin Liliensiek et al. (2013) β€” Heterologous Overexpression, Purification and Characterisation of an Alcohol Dehydrogenase (ADH2) from Halobacterium sp. NRC-1
Molecular Biotechnology  Β· doi:10.1007/s12033-013-9666-4 β†—  Β· Stability - Incubation
12 measurements ▼

Structure

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