Lipase A from Proteus sp. SW1

Enzyme Description

Species
Proteus sp. SW1 β†—
Extremophile
No but "organic-solvent tolerant" enzyme
EC Number
3.1.1.3 β†—

Sequence

Length: 287 amino acids
MPTTYPIVLVHGLSGFDNVVGYPYFYGIADALEKDGHKVFTASLSAFNANEVRGEQLWGFVQKVLKETKAKKVNLIGHSQGPLACRYVAAKHAKNIASVTSINGVNHGSEIADLVRRIVRKDSVPEYIADAVMKAIGTIISTFSGHRSNPQDAVAALEALTTENVTEFNKKYPQGLPAIRGGEGKEVVNGVYYYSFGSYIQGLIVGEKGNLLDPTHAAMRVLSAFFTEHENDGLVGRTSMRLGKLIKDDYAEDHLDMVNQVAGLVGRGEDIVAIYTNHANFLASKKL
Wirongrong Whangsuk et al. (2012) β€” Gene Cloning and Characterization of a Novel Highly Organic Solvent Tolerant Lipase from Proteus sp. SW1 and its Application for Biodiesel Production
Molecular Biotechnology  Β· doi:10.1007/s12033-012-9518-7 β†—  Β· Stability - Incubation
39 measurements ▼
Database ID
UniProt: F2Y9R8 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (39 measurements)

39 measurements β€” page 1 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (3 hours at 55Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase n-Hexane 60% 0.0 1.8 % 50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic Incubation: 8, Assay: 8 Incubation: 55Β°C, Assay: 28Β°C 0.05 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), activity in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (4.5 hours at 28Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 60% 100.0 324.0 % 50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic Incubation: 8, Assay: 8 Incubation: 28Β°C, Assay: 28Β°C 0.05 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), reaction in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (4.5 hours at 28Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetone 60% 100.0 969.0 % 50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic Incubation: 8, Assay: 8 Incubation: 28Β°C, Assay: 28Β°C 0.05 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), reaction in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (6 hours at 28Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 60% 100.0 248.0 % 50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic Incubation: 8, Assay: 8 Incubation: 28Β°C, Assay: 28Β°C 0.05 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), reaction in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (6 hours at 28Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetone 60% 100.0 887.0 % 50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic Incubation: 8, Assay: 8 Incubation: 28Β°C, Assay: 28Β°C 0.05 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), reaction in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (0.5 hour at 55Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetone 60% 0.0 3.3 % 50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic Incubation: 8, Assay: 8 Incubation: 55Β°C, Assay: 28Β°C 0.05 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), activity in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (1.5 hour at 55Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetone 60% 0.0 4.3 % 50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic Incubation: 8, Assay: 8 Incubation: 55Β°C, Assay: 28Β°C 0.05 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), activity in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (3 hours at 55Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetone 60% 0.0 0.3 % 50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic Incubation: 8, Assay: 8 Incubation: 55Β°C, Assay: 28Β°C 0.05 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), activity in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (0.5 hour at 55Β°C), measured by absorbance spectrophotometry n-Hexane 60% 0.0 3.0 % 50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic Incubation: 8, Assay: 8 Incubation: 55Β°C, Assay: 28Β°C 0.05 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), activity in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (1.5 hour at 55Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase n-Hexane 60% 0.0 2.6 % 50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic Incubation: 8, Assay: 8 Incubation: 55Β°C, Assay: 28Β°C 0.05 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), activity in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (3 hours at 28Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetone 60% 100.0 1188.0 % 50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic Incubation: 8, Assay: 8 Incubation: 28Β°C, Assay: 28Β°C 0.05 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), reaction in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (0.5 hour at 55Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Methanol 60% 0.0 4.8 % 50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic Incubation: 8, Assay: 8 Incubation: 55Β°C, Assay: 28Β°C 0.05 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), activity in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (1.5 hour at 55Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Methanol 60% 0.0 2.3 % 50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic Incubation: 8, Assay: 8 Incubation: 55Β°C, Assay: 28Β°C 0.05 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), activity in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (3 hours at 55Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Methanol 60% 0.0 0.0 % 50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic Incubation: 8, Assay: 8 Incubation: 55Β°C, Assay: 28Β°C 0.05 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), activity in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (0.5 hour at 55Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 60% 0.0 0.0 % 50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic Incubation: 8, Assay: 8 Incubation: 55Β°C, Assay: 28Β°C 0.05 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), activity in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (1.5 hour at 55Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 60% 0.0 0.0 % 50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic Incubation: 8, Assay: 8 Incubation: 55Β°C, Assay: 28Β°C 0.05 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), activity in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (3 hours at 55Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 60% 0.0 0.0 % 50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic Incubation: 8, Assay: 8 Incubation: 55Β°C, Assay: 28Β°C 0.05 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), activity in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (0.5 hour at 55Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Isopropanol 60% 0.0 0.0 % 50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic Incubation: 8, Assay: 8 Incubation: 55Β°C, Assay: 28Β°C 0.05 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), activity in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (1.5 hour at 55Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Isopropanol 60% 0.0 0.0 % 50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic Incubation: 8, Assay: 8 Incubation: 55Β°C, Assay: 28Β°C 0.05 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), activity in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (3 hours at 55Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Isopropanol 60% 0.0 0.0 % 50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic Incubation: 8, Assay: 8 Incubation: 55Β°C, Assay: 28Β°C 0.05 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Water-incubated control (in %), activity in aqueous phase | Uncertainty about whether assay done with or without organic solvent
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Visualization : Stability β€” Incubation

Wirongrong Whangsuk et al. (2012)

One bar per measurement. Colour = solvent, shade = solvent volume.

Yu Wang et al. (2018) β€” High-level expression and characterization of solvent-tolerant lipase
Journal of Bioscience and Bioengineering  Β· doi:10.1016/j.jbiosc.2017.06.012 β†—  Β· Stability - Incubation
34 measurements ▼

Structure

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