Lipase A from Proteus sp. SW1

Enzyme Description

Species

Proteus sp. SW1 ↗

Extremophile

No but "organic-solvent tolerant" enzyme

EC Number

3.1.1.3 ↗

Sequence

Length: 287 amino acids

MPTTYPIVLVHGLSGFDNVVGYPYFYGIADALEKDGHKVFTASLSAFNANEVRGEQLWGFVQKVLKETKAKKVNLIGHSQGPLACRYVAAKHAKNIASVTSINGVNHGSEIADLVRRIVRKDSVPEYIADAVMKAIGTIISTFSGHRSNPQDAVAALEALTTENVTEFNKKYPQGLPAIRGGEGKEVVNGVYYYSFGSYIQGLIVGEKGNLLDPTHAAMRVLSAFFTEHENDGLVGRTSMRLGKLIKDDYAEDHLDMVNQVAGLVGRGEDIVAIYTNHANFLASKKL

Wirongrong Whangsuk et al. (2012) — Gene Cloning and Characterization of a Novel Highly Organic Solvent Tolerant Lipase from Proteus sp. SW1 and its Application for Biodiesel Production

Molecular Biotechnology · doi:10.1007/s12033-012-9518-7 ↗ · Stability - Incubation

39 measurements ▼

Database ID

UniProt: F2Y9R8 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (39 measurements)

39 measurements — page 1 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (3 hours at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	n-Hexane	60%	0.0	1.8	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 55°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (4.5 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	60%	100.0	324.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 28°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), reaction in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (4.5 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	60%	100.0	969.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 28°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), reaction in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (6 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	60%	100.0	248.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 28°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), reaction in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (6 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	60%	100.0	887.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 28°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), reaction in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (0.5 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	60%	0.0	3.3	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 55°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (1.5 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	60%	0.0	4.3	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 55°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (3 hours at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	60%	0.0	0.3	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 55°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (0.5 hour at 55°C), measured by absorbance spectrophotometry	n-Hexane	60%	0.0	3.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 55°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (1.5 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	n-Hexane	60%	0.0	2.6	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 55°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (3 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	60%	100.0	1188.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 28°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), reaction in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (0.5 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	60%	0.0	4.8	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 55°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (1.5 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	60%	0.0	2.3	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 55°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (3 hours at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	60%	0.0	0.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 55°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (0.5 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	60%	0.0	0.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 55°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (1.5 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	60%	0.0	0.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 55°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (3 hours at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	60%	0.0	0.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 55°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (0.5 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isopropanol	60%	0.0	0.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 55°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (1.5 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isopropanol	60%	0.0	0.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 55°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (3 hours at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isopropanol	60%	0.0	0.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 55°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether assay done with or without organic solvent

Stability - Incubation	Activity after incubation (3 hours at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	n-Hexane	60%	0.0	1.8	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 55°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (4.5 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	60%	100.0	324.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 28°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), reaction in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (4.5 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	60%	100.0	969.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 28°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), reaction in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (6 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	60%	100.0	248.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 28°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), reaction in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (6 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	60%	100.0	887.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 28°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), reaction in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (0.5 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	60%	0.0	3.3	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 55°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (1.5 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	60%	0.0	4.3	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 55°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (3 hours at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	60%	0.0	0.3	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 55°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (0.5 hour at 55°C), measured by absorbance spectrophotometry	n-Hexane	60%	0.0	3.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 55°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (1.5 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	n-Hexane	60%	0.0	2.6	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 55°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (3 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	60%	100.0	1188.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 28°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), reaction in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (0.5 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	60%	0.0	4.8	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 55°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (1.5 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	60%	0.0	2.3	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 55°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (3 hours at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	60%	0.0	0.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 55°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (0.5 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	60%	0.0	0.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 55°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (1.5 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	60%	0.0	0.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 55°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (3 hours at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	60%	0.0	0.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 55°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (0.5 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isopropanol	60%	0.0	0.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 55°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (1.5 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isopropanol	60%	0.0	0.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 55°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (3 hours at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isopropanol	60%	0.0	0.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 55°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity in aqueous phase \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (0.5 hour at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isopropanol	60%	100.0	772.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 28°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), reaction in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (18 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	60%	100.0	235.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 28°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), reaction in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (0.5 hour at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	n-Hexane	60%	100.0	568.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 28°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), reaction in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (18 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	n-Hexane	60%	100.0	191.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 28°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), reaction in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (0.5 hour at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	60%	100.0	204.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 28°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), reaction in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (18 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	60%	100.0	30.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 28°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), reaction in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (0.5 hour at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	60%	100.0	99.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 28°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), reaction in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (18 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	60%	100.0	236.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 28°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), reaction in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (0.5 hour at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	100%	100.0	140.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 28°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), reaction in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (18 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	100%	100.0	24.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 28°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), reaction in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (0.5 hour at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	60%	100.0	40.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 28°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), reaction in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (18 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isopropanol	60%	100.0	245.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 28°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), reaction in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (0.5 hour at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isopropanol	100%	100.0	123.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 28°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), reaction in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (18 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isopropanol	100%	100.0	108.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 28°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), reaction in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (0.5 hour at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	60%	100.0	112.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 28°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), reaction in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (0.5 hour at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	60%	100.0	68.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 28°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), reaction in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (1.5 hour at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	60%	100.0	679.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 28°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), reaction in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (1.5 hour at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	60%	100.0	1184.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 28°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), reaction in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Stability - Incubation	Activity after incubation (3 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	60%	100.0	502.0	%	50 mM phosphate buffer incubation, 20 mM Tris–HCl, 150 mM NaCl, 0.01% v/v Triton X-100, and 0.01% w/v Gum Arabic	Incubation: 8, Assay: 8	Incubation: 28°C, Assay: 28°C	0.05 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), reaction in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent

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Visualization : Stability — Incubation

Wirongrong Whangsuk et al. (2012)

One bar per measurement. Colour = solvent, shade = solvent volume.

Yu Wang et al. (2018) — High-level expression and characterization of solvent-tolerant lipase

Journal of Bioscience and Bioengineering · doi:10.1016/j.jbiosc.2017.06.012 ↗ · Stability - Incubation

34 measurements ▼

Database ID

UniProt: F2Y9R8 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host yeast Pichia pastoris GS115

Experimental Data (34 measurements)

34 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (24 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	80%	100	104	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (3 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	80%	100	153	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (24 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	80%	100	145	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (3 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isopropanol	80%	100	163	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (24 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isopropanol	80%	100	124	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (3 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetonitrile	80%	100	157	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (3 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	80%	100	66	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (3 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	n-Hexane	80%	100	22	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (24 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	n-Hexane	80%	100	1	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (3 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Benzene	80%	100	16	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (24 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Benzene	80%	100	0	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (2 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	10%	100	60	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (2 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	20%	100	38	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (2 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	30%	100	44	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear

Stability - Incubation	Activity after incubation (2 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	30%	100	38	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (2 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	50%	100	44	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (2 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	60%	100	74	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (2 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	70%	100	116	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (2 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	80%	100	126	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (2 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	90%	100	118	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (2 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	98%	100	117	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (2 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	10%	100	25	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (2 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	20%	100	42	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (2 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	40%	100	45	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (2 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	40%	100	53	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (2 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	50%	100	66	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (2 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	60%	100	154	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (2 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	70%	100	164	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (2 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	80%	100	191	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (2 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	90%	100	138	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (2 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	98%	100	112	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (24 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetonitrile	80%	100	104	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (24 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	80%	100	37	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (3 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	80%	100	106	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (24 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	80%	100	104	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (3 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	80%	100	153	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (24 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	80%	100	145	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (3 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isopropanol	80%	100	163	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (24 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isopropanol	80%	100	124	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (3 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetonitrile	80%	100	157	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (3 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	80%	100	66	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (3 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	n-Hexane	80%	100	22	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (24 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	n-Hexane	80%	100	1	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (3 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Benzene	80%	100	16	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (24 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Benzene	80%	100	0	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (2 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	10%	100	60	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (2 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	20%	100	38	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear
Stability - Incubation	Activity after incubation (2 hours at 28°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	30%	100	44	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 5% isopropanol	Incubation: ?, Assay: 8	Incubation: 28°C, Assay: 40°C	0.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear

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Visualization : Stability — Incubation

Yu Wang et al. (2018)

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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Lipase A from Proteus sp. SW1

Enzyme Description

Sequence

Experimental Data (39 measurements)

Visualization : Stability — Incubation

Experimental Data (34 measurements)

Visualization : Stability — Incubation

Structure

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