Lipase (gene lipA) from Sorangium cellulosum So0157-2

Enzyme Description

Species
Sorangium cellulosum So0157-2 β†—
Extremophile
No but"cold-adapted" enzyme
EC Number
3.1.1.3 β†—

Sequence

Length: 326 amino acids
MPADTFTFQADDGRELFVYRWLPDDGARGIFHIAHGMSEHAGRYARLAHALSAAGWAVYANDHRGHGRTARERGELGFFASQGGFQRVVRDLAALIAREQEAHPGLPVVLFGHSMGSSLVQEYLIERGGSIQGAVLSGSSGKPSPLVDAGRLVARAERRRLGERGKSQLLQSMSFDSFNKLFAPARTPFDWLSRDRAEVDGYIADPCCGFPATVSLWIDLLDAMVDIARPERQARIPKDLPVYVFSGSRDPVGGPLRSVTQMIEAYRAAGLRRVTPRIYPGGRHEMLNEINREEVVRDLLAWLDAEVKTEAAPARPARPSAGAAAG
Yuan-Yuan Cheng et al. (2011) β€” A Novel Cold-Adapted Lipase from Sorangium cellulosum Strain So0157-2: Gene Cloning, Expression, and Enzymatic Characterization
International Journal of Molecular Sciences  Β· doi:10.3390/ijms12106765 β†—  Β· Stability - Incubation
32 measurements ▼
Database ID
UniProt: G8E7B4 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (32 measurements)

32 measurements β€” page 2 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Cyclohexane 25% 100.0 156.0 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 1% Ethanol Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 0.5 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase p-Xylene 25% 100.0 153.7 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 1% Ethanol Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 0.5 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Toluene 25% 100.0 150.9 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 1% Ethanol Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 0.5 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Benzene 25% 100.0 140.5 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 1% Ethanol Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 0.5 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Chloroform 25% 100.0 139.0 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 1% Ethanol Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 0.5 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Diethyl Ether 25% 100.0 113.7 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 1% Ethanol Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 0.5 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase 1-Butanol 25% 100.0 99.2 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 1% Ethanol Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 0.5 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase 1-Propanol 25% 100.0 76.3 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 1% Ethanol Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 0.5 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Isopropanol 25% 100.0 57.2 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 1% Ethanol Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 0.5 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetone 25% 100.0 78.8 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 1% Ethanol Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 0.5 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 25% 100.0 75.4 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 1% Ethanol Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 0.5 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Methanol 25% 100.0 97.7 % Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 1% Ethanol Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 0.5 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent
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Visualization : Stability β€” Incubation

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Structure

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