Lipase (gene lipA) from Sorangium cellulosum So0157-2
Enzyme Description
Extremophile
No
but"cold-adapted" enzyme
EC Number
Sequence
MPADTFTFQADDGRELFVYRWLPDDGARGIFHIAHGMSEHAGRYARLAHALSAAGWAVYANDHRGHGRTARERGELGFFASQGGFQRVVRDLAALIAREQEAHPGLPVVLFGHSMGSSLVQEYLIERGGSIQGAVLSGSSGKPSPLVDAGRLVARAERRRLGERGKSQLLQSMSFDSFNKLFAPARTPFDWLSRDRAEVDGYIADPCCGFPATVSLWIDLLDAMVDIARPERQARIPKDLPVYVFSGSRDPVGGPLRSVTQMIEAYRAAGLRRVTPRIYPGGRHEMLNEINREEVVRDLLAWLDAEVKTEAAPARPARPSAGAAAG
Yuan-Yuan Cheng et al. (2011)
β A Novel Cold-Adapted Lipase from Sorangium cellulosum Strain So0157-2: Gene Cloning, Expression, and Enzymatic Characterization
International Journal of Molecular Sciences
Β· doi:10.3390/ijms12106765 β
Β· Stability - Incubation
▼
Database ID
UniProt: G8E7B4 β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (32 measurements)
32 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (2 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 25% | 100.0 | 88.1 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent |
| Stability - Incubation | Activity after incubation (2 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Isooctane | 25% | 100.0 | 132.8 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent |
| Stability - Incubation | Activity after incubation (2 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | n-Heptane | 25% | 100.0 | 167.1 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent |
| Stability - Incubation | Activity after incubation (2 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | n-Hexane | 25% | 100.0 | 130.8 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent |
| Stability - Incubation | Activity after incubation (2 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Cyclohexane | 25% | 100.0 | 136.9 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent |
| Stability - Incubation | Activity after incubation (2 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | p-Xylene | 25% | 100.0 | 152.9 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent |
| Stability - Incubation | Activity after incubation (2 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Toluene | 25% | 100.0 | 159.2 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent |
| Stability - Incubation | Activity after incubation (2 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Benzene | 25% | 100.0 | 160.2 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent |
| Stability - Incubation | Activity after incubation (2 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Chloroform | 25% | 100.0 | 142.8 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent |
| Stability - Incubation | Activity after incubation (2 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Diethyl Ether | 25% | 100.0 | 118.5 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent |
| Stability - Incubation | Activity after incubation (2 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | 1-Butanol | 25% | 100.0 | 82.6 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent |
| Stability - Incubation | Activity after incubation (2 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | 1-Propanol | 25% | 100.0 | 50.2 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent |
| Stability - Incubation | Activity after incubation (2 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Isopropanol | 25% | 100.0 | 46.6 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent |
| Stability - Incubation | Activity after incubation (2 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Acetone | 25% | 100.0 | 69.2 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent |
| Stability - Incubation | Activity after incubation (2 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Ethanol | 25% | 100.0 | 67.6 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent |
| Stability - Incubation | Activity after incubation (2 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Methanol | 25% | 100.0 | 90.2 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 25% | 100.0 | 97.1 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Isooctane | 25% | 100.0 | 163.7 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | n-Heptane | 25% | 100.0 | 159.1 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | n-Hexane | 25% | 100.0 | 153.0 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay done with or without organic solvent |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.