Ene-reductase KYE1 from Kluyveromyces lactis

Enzyme Description

Species

Extremophile

EC Number

1.3.1.31 ↗

Sequence

Length: 398 amino acids

MSFMNFEPKPLADTDIFKPIKIGNTELKHRVVMPALTRMRALHPGNVPNPDWAVEYYRQRSQYPGTMIITEGAFPSAQSGGYDNAPGVWSEEQLAQWRKIFKAIHDNKSFVWVQLWVLGRQAFADNLARDGLRYDSASDEVYMGEDEKERAIRSNNPQHGITKDEIKQYIRDYVDAAKKCIDAGADGVEIHSANGYLLNQFLDPISNKRTDEYGGSIENRARFVLEVVDAVVDAVGAERTSIRFSPYGVFGTMSGGSDPVLVAQFAYVLAELEKRAKAGKRLAYVDLVEPRVTSPFQPEFEGWYKGGTNEFVYSVWKGNVLRVGNYALDPDAAITDSKNPNTLIGYGRAFIANPDLVERLEKGLPLNQYDRPSFYKMSAEGYIDYPTYEEAVAKGYKK

Yanto Yanto et al. (2011) — Asymmetric bioreduction of alkenes using ene-reductases YersER and KYE1 and effects of organic solvents

Organic Letters · doi:10.1021/ol200394p ↗ · Stability - C50 Activity + Stability - Conversion Specificity - Enantioselectivity

84 measurements ▼

Database ID

UniProt: P40952 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21

Experimental Data (84 measurements)

84 measurements — page 5 of 5

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Specificity - Enantioselectivity	Enantiomeric excess (toward the R form) after incubation (12 hours at 30°C), measured by gas chromatography	Ethylene Glycol	20%	86.0	82.0	%	200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)	7.5	30°C	10 mM Citral	Citronellal	0.2 mM NADP+	—	Classical aqueous control (in %)
Specificity - Enantioselectivity	Enantiomeric excess (toward the R form) after incubation (12 hours at 30°C), measured by gas chromatography	Ethylene Glycol	10%	86.0	84.0	%	200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)	7.5	30°C	10 mM Citral	Citronellal	0.2 mM NADP+	—	Classical aqueous control (in %)
Stability - C50	Concentration (in % v/v) for which 50% of enzyme activity, assessed by incubation (12 hours at 30°C) in the presence of organic solvent and measured by gas chromatography	Ethylene Glycol	—	—	43.3	% (v/v)	200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)	7.5	30°C	10 mM 2-Cyclohexen-1-one	Cyclohexanone	0.2 mM NADP+	—	Not applicable control (in % (v/v))
Stability - C50	Concentration (in % v/v) for which 50% of enzyme activity, assessed by incubation (12 hours at 30°C) in the presence of organic solvent and measured by gas chromatography	Dimethyl Sulfoxide (DMSO)	—	—	27.5	% (v/v)	200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)	7.5	30°C	10 mM 2-Cyclohexen-1-one	Cyclohexanone	0.2 mM NADP+	—	Not applicable control (in % (v/v))

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Ethylene Glycol

50%

67.0

35.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Methanol

20%

100.0

23.7

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Propylene Glycol

20%

100.0

3.2

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Ethanol

20%

100.0

6.6

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Dimethyl Sulfoxide (DMSO)

20%

100.0

98.5

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Dimethylformamide (DMF)

20%

100.0

4.1

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Acetonitrile

20%

100.0

0.2

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Acetone

20%

100.0

5.3

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

1,4-Dioxane

20%

100.0

9.1

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Tetrahydrofuran (THF)

20%

100.0

0.7

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Toluene

20%

100.0

97.8

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

n-Hexane

20%

100.0

95.3

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Ethylene Glycol

10%

67.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Ethylene Glycol

20%

67.0

61.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Ethylene Glycol

30%

67.0

62.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Ethylene Glycol

40%

67.0

50.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Ethylene Glycol

20%

100.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Dimethyl Sulfoxide (DMSO)

10%

67.0

70.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Dimethyl Sulfoxide (DMSO)

20%

67.0

51.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Dimethyl Sulfoxide (DMSO)

30%

67.0

21.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Dimethyl Sulfoxide (DMSO)

40%

67.0

16.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Dimethyl Sulfoxide (DMSO)

50%

67.0

15.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

n-Hexane

10%

67.0

63.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

n-Hexane

20%

67.0

65.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

n-Hexane

30%

67.0

60.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

n-Hexane

40%

67.0

66.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

n-Hexane

50%

67.0

62.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Toluene

10%

67.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Toluene

20%

67.0

68.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Toluene

30%

67.0

69.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Toluene

40%

67.0

63.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Toluene

50%

67.0

64.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

n-Hexane

20%

100.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Ethylene Glycol

80%

100.0

0.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Ethylene Glycol

70%

100.0

0.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Dimethyl Sulfoxide (DMSO)

10%

100.0

89.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Dimethyl Sulfoxide (DMSO)

20%

100.0

90.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Ethylene Glycol

60%

100.0

7.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Dimethyl Sulfoxide (DMSO)

25%

100.0

61.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Dimethyl Sulfoxide (DMSO)

30%

100.0

22.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Ethylene Glycol

50%

100.0

12.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Dimethyl Sulfoxide (DMSO)

40%

100.0

0.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Dimethyl Sulfoxide (DMSO)

45%

100.0

0.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Ethylene Glycol

45%

100.0

28.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Dimethyl Sulfoxide (DMSO)

50%

100.0

0.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

n-Hexane

10%

100.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Ethylene Glycol

40%

100.0

91.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

n-Hexane

30%

100.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Toluene

10%

100.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Ethylene Glycol

20%

100.0

98.9

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Ethylene Glycol

10%

100.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Toluene

70%

100.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Toluene

60%

100.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Toluene

50%

100.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Toluene

40%

100.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Toluene

30%

100.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Toluene

20%

100.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

Ethylene Glycol

30%

100.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

n-Hexane

70%

100.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

n-Hexane

60%

100.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

n-Hexane

50%

100.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion after incubation (12 hours at 30°C) in the presence of organic solvent, measured by gas chromatography

n-Hexane

40%

100.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Classical aqueous control (in %)

Specificity - Enantioselectivity

Enantiomeric excess (toward the R form) after incubation (12 hours at 30°C), measured by gas chromatography

Toluene

10%

86.0

80.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Specificity - Enantioselectivity

Enantiomeric excess (toward the R form) after incubation (12 hours at 30°C), measured by gas chromatography

n-Hexane

50%

86.0

84.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Specificity - Enantioselectivity

Enantiomeric excess (toward the R form) after incubation (12 hours at 30°C), measured by gas chromatography

Toluene

20%

86.0

83.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Specificity - Enantioselectivity

Enantiomeric excess (toward the R form) after incubation (12 hours at 30°C), measured by gas chromatography

Toluene

40%

86.0

83.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Specificity - Enantioselectivity

Enantiomeric excess (toward the R form) after incubation (12 hours at 30°C), measured by gas chromatography

n-Hexane

40%

86.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Specificity - Enantioselectivity

Enantiomeric excess (toward the R form) after incubation (12 hours at 30°C), measured by gas chromatography

Toluene

50%

86.0

83.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Specificity - Enantioselectivity

Enantiomeric excess (toward the R form) after incubation (12 hours at 30°C), measured by gas chromatography

Toluene

30%

86.0

82.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Specificity - Enantioselectivity

Enantiomeric excess (toward the R form) after incubation (12 hours at 30°C), measured by gas chromatography

n-Hexane

30%

86.0

84.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Specificity - Enantioselectivity

Enantiomeric excess (toward the R form) after incubation (12 hours at 30°C), measured by gas chromatography

n-Hexane

20%

86.0

82.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Specificity - Enantioselectivity

Enantiomeric excess (toward the R form) after incubation (12 hours at 30°C), measured by gas chromatography

n-Hexane

10%

86.0

89.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Specificity - Enantioselectivity

Enantiomeric excess (toward the R form) after incubation (12 hours at 30°C), measured by gas chromatography

Dimethyl Sulfoxide (DMSO)

50%

86.0

54.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Specificity - Enantioselectivity

Enantiomeric excess (toward the R form) after incubation (12 hours at 30°C), measured by gas chromatography

Dimethyl Sulfoxide (DMSO)

40%

86.0

70.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Specificity - Enantioselectivity

Enantiomeric excess (toward the R form) after incubation (12 hours at 30°C), measured by gas chromatography

Dimethyl Sulfoxide (DMSO)

30%

86.0

76.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Specificity - Enantioselectivity

Enantiomeric excess (toward the R form) after incubation (12 hours at 30°C), measured by gas chromatography

Dimethyl Sulfoxide (DMSO)

20%

86.0

81.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Specificity - Enantioselectivity

Enantiomeric excess (toward the R form) after incubation (12 hours at 30°C), measured by gas chromatography

Dimethyl Sulfoxide (DMSO)

10%

86.0

82.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Specificity - Enantioselectivity

Enantiomeric excess (toward the R form) after incubation (12 hours at 30°C), measured by gas chromatography

Ethylene Glycol

50%

86.0

60.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Specificity - Enantioselectivity

Enantiomeric excess (toward the R form) after incubation (12 hours at 30°C), measured by gas chromatography

Ethylene Glycol

40%

86.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Specificity - Enantioselectivity

Enantiomeric excess (toward the R form) after incubation (12 hours at 30°C), measured by gas chromatography

Ethylene Glycol

30%

86.0

87.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Specificity - Enantioselectivity

Enantiomeric excess (toward the R form) after incubation (12 hours at 30°C), measured by gas chromatography

Ethylene Glycol

20%

86.0

82.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Specificity - Enantioselectivity

Enantiomeric excess (toward the R form) after incubation (12 hours at 30°C), measured by gas chromatography

Ethylene Glycol

10%

86.0

84.0

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM Citral

Citronellal

0.2 mM NADP+

—

Classical aqueous control (in %)

Stability - C50

Concentration (in % v/v) for which 50% of enzyme activity, assessed by incubation (12 hours at 30°C) in the presence of organic solvent and measured by gas chromatography

Ethylene Glycol

—

43.3

% (v/v)

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Not applicable control (in % (v/v))

Stability - C50

Concentration (in % v/v) for which 50% of enzyme activity, assessed by incubation (12 hours at 30°C) in the presence of organic solvent and measured by gas chromatography

Dimethyl Sulfoxide (DMSO)

—

27.5

% (v/v)

200 mM phosphate buffern 20 mM glucose, 10 U/ml GDH (cofactor recycling)

7.5

30°C

10 mM 2-Cyclohexen-1-one

Cyclohexanone

0.2 mM NADP+

—

Not applicable control (in % (v/v))

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Visualization : Stability — C₅₀

One bar per measurement. Colour = solvent, shade = temperature. Hover for details.

Visualization : Activity + Stability — Conversion

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Specificity — Enantioselectivity

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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Ene-reductase KYE1 from Kluyveromyces lactis

Enzyme Description

Sequence

Experimental Data (84 measurements)

Visualization : Stability — C50

Visualization : Activity + Stability — Conversion

Visualization : Specificity — Enantioselectivity

Structure

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Visualization : Stability — C₅₀