Lipase (gene Lip-SBRN2) from Bacillus sp. RN2

Enzyme Description

Species

Bacillus sp. RN2 ↗

Extremophile

Yes thermophilic organism

EC Number

3.1.1.3 ↗

Sequence

Length: 191 amino acids

MLSVVSVFSFRPSAASAASHNPVVMVHGIGGADYNFIGIKSYLQSQGWTSSELYAINFIDKTGNNINNAPRLSEYIKRVLNQTGASKVDIVAHSMGGANTLYYIKNLDGADKVGHVVTLGGANRLVTNTAPQNDKISYTSIYSTSDYIVLNSLSKLDGANNVQISGVSHVGLLFNNKVNALIKDGLTASGK

Pornpimon Kanjanavas et al. (2010) — Purification and Characterization of Organic Solvent and Detergent Tolerant Lipase from Thermotolerant Bacillus sp. RN2

International Journal of Molecular Sciences · doi:10.3390/ijms11103783 ↗ · Activity + Stability - Incubation

22 measurements ▼

Database ID

UniProt: B3F2Y4 ↗

Sequence Annotation

Inferred

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (22 measurements)

22 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity + Stability - Incubation	Activity after incubation (30 minutes at 37°C) measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in the presence of organic solvent	Acetone	25%	100.0	100.8	%	Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2	Incubation: 8, Assay: 8	Incubation: 37°C, Assay: 37°C	emulsion : 70% v/v Olive oil	free fatty acids , glycerol	—	Incubation: 150 rpm	Water-incubated control (in %), activity measured in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 minutes at 37°C) measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in the presence of organic solvent	Diethyl Ether	25%	100.0	102.4	%	Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2	Incubation: 8, Assay: 8	Incubation: 37°C, Assay: 37°C	emulsion : 70% v/v Olive oil	free fatty acids , glycerol	—	Incubation: 150 rpm	Water-incubated control (in %), activity measured in the presence of organic solvent

Activity + Stability - Incubation

Activity after incubation (2 hours at 37°C) measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in the presence of organic solvent

Benzene

25%

100.0

104.4

Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 37°C, Assay: 37°C

emulsion : 70% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Water-incubated control (in %), activity measured in the presence of organic solvent

Activity + Stability - Incubation

Isopropanol

25%

100.0

66.5

Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 37°C, Assay: 37°C

emulsion : 70% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Water-incubated control (in %), activity measured in the presence of organic solvent

Activity + Stability - Incubation

Toluene

25%

100.0

80.4

Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 37°C, Assay: 37°C

emulsion : 70% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Water-incubated control (in %), activity measured in the presence of organic solvent

Activity + Stability - Incubation

Acetonitrile

25%

100.0

82.5

Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 37°C, Assay: 37°C

emulsion : 70% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Water-incubated control (in %), activity measured in the presence of organic solvent

Activity + Stability - Incubation

Chloroform

25%

100.0

84.4

Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 37°C, Assay: 37°C

emulsion : 70% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Water-incubated control (in %), activity measured in the presence of organic solvent

Activity + Stability - Incubation

Dimethylformamide (DMF)

25%

100.0

86.9

Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 37°C, Assay: 37°C

emulsion : 70% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Water-incubated control (in %), activity measured in the presence of organic solvent

Activity + Stability - Incubation

Cyclohexane

25%

100.0

85.1

Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 37°C, Assay: 37°C

emulsion : 70% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Water-incubated control (in %), activity measured in the presence of organic solvent

Activity + Stability - Incubation

Dimethyl Sulfoxide (DMSO)

25%

100.0

96.4

Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 37°C, Assay: 37°C

emulsion : 70% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Water-incubated control (in %), activity measured in the presence of organic solvent

Activity + Stability - Incubation

n-Hexane

25%

100.0

94.5

Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 37°C, Assay: 37°C

emulsion : 70% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Water-incubated control (in %), activity measured in the presence of organic solvent

Activity + Stability - Incubation

Acetone

25%

100.0

82.2

Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 37°C, Assay: 37°C

emulsion : 70% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Water-incubated control (in %), activity measured in the presence of organic solvent

Activity + Stability - Incubation

Diethyl Ether

25%

100.0

103.6

Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 37°C, Assay: 37°C

emulsion : 70% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Water-incubated control (in %), activity measured in the presence of organic solvent

Activity + Stability - Incubation

Activity after incubation (30 minutes at 37°C) measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in the presence of organic solvent

Benzene

25%

100.0

108.0

Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 37°C, Assay: 37°C

emulsion : 70% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Water-incubated control (in %), activity measured in the presence of organic solvent

Activity + Stability - Incubation

Isopropanol

25%

100.0

60.8

Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 37°C, Assay: 37°C

emulsion : 70% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Water-incubated control (in %), activity measured in the presence of organic solvent

Activity + Stability - Incubation

Toluene

25%

100.0

68.4

Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 37°C, Assay: 37°C

emulsion : 70% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Water-incubated control (in %), activity measured in the presence of organic solvent

Activity + Stability - Incubation

Acetonitrile

25%

100.0

69.6

Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 37°C, Assay: 37°C

emulsion : 70% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Water-incubated control (in %), activity measured in the presence of organic solvent

Activity + Stability - Incubation

Chloroform

25%

100.0

80.0

Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 37°C, Assay: 37°C

emulsion : 70% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Water-incubated control (in %), activity measured in the presence of organic solvent

Activity + Stability - Incubation

Dimethylformamide (DMF)

25%

100.0

80.4

Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 37°C, Assay: 37°C

emulsion : 70% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Water-incubated control (in %), activity measured in the presence of organic solvent

Activity + Stability - Incubation

Cyclohexane

25%

100.0

84.4

Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 37°C, Assay: 37°C

emulsion : 70% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Water-incubated control (in %), activity measured in the presence of organic solvent

Activity + Stability - Incubation

Dimethyl Sulfoxide (DMSO)

25%

100.0

85.2

Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 37°C, Assay: 37°C

emulsion : 70% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Water-incubated control (in %), activity measured in the presence of organic solvent

Activity + Stability - Incubation

n-Hexane

25%

100.0

94.0

Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 37°C, Assay: 37°C

emulsion : 70% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Water-incubated control (in %), activity measured in the presence of organic solvent

Activity + Stability - Incubation

Acetone

25%

100.0

100.8

Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 37°C, Assay: 37°C

emulsion : 70% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Water-incubated control (in %), activity measured in the presence of organic solvent

Activity + Stability - Incubation

Diethyl Ether

25%

100.0

102.4

Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 37°C, Assay: 37°C

emulsion : 70% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Water-incubated control (in %), activity measured in the presence of organic solvent

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Visualization : Activity + Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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Lipase (gene Lip-SBRN2) from Bacillus sp. RN2

Enzyme Description

Sequence

Experimental Data (22 measurements)

Visualization : Activity + Stability — Incubation

Structure

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