Lipase (gene Lip-SBRN2) from Bacillus sp. RN2
Enzyme Description
Sequence
MLSVVSVFSFRPSAASAASHNPVVMVHGIGGADYNFIGIKSYLQSQGWTSSELYAINFIDKTGNNINNAPRLSEYIKRVLNQTGASKVDIVAHSMGGANTLYYIKNLDGADKVGHVVTLGGANRLVTNTAPQNDKISYTSIYSTSDYIVLNSLSKLDGANNVQISGVSHVGLLFNNKVNALIKDGLTASGK
Pornpimon Kanjanavas et al. (2010)
β Purification and Characterization of Organic Solvent and Detergent Tolerant Lipase from Thermotolerant Bacillus sp. RN2
International Journal of Molecular Sciences
Β· doi:10.3390/ijms11103783 β
Β· Activity + Stability - Incubation
▼
Database ID
UniProt: B3F2Y4 β
Sequence Annotation
Inferred
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (22 measurements)
22 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity + Stability - Incubation | Activity after incubation (2 hours at 37Β°C) measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | Benzene | 25% | 100.0 | 104.4 | % | Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2 | Incubation: 8, Assay: 8 | Incubation: 37Β°C, Assay: 37Β°C | emulsion : 70% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent |
| Activity + Stability - Incubation | Activity after incubation (2 hours at 37Β°C) measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | Isopropanol | 25% | 100.0 | 66.5 | % | Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2 | Incubation: 8, Assay: 8 | Incubation: 37Β°C, Assay: 37Β°C | emulsion : 70% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent |
| Activity + Stability - Incubation | Activity after incubation (2 hours at 37Β°C) measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | Toluene | 25% | 100.0 | 80.4 | % | Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2 | Incubation: 8, Assay: 8 | Incubation: 37Β°C, Assay: 37Β°C | emulsion : 70% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent |
| Activity + Stability - Incubation | Activity after incubation (2 hours at 37Β°C) measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | Acetonitrile | 25% | 100.0 | 82.5 | % | Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2 | Incubation: 8, Assay: 8 | Incubation: 37Β°C, Assay: 37Β°C | emulsion : 70% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent |
| Activity + Stability - Incubation | Activity after incubation (2 hours at 37Β°C) measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | Chloroform | 25% | 100.0 | 84.4 | % | Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2 | Incubation: 8, Assay: 8 | Incubation: 37Β°C, Assay: 37Β°C | emulsion : 70% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent |
| Activity + Stability - Incubation | Activity after incubation (2 hours at 37Β°C) measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 25% | 100.0 | 86.9 | % | Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2 | Incubation: 8, Assay: 8 | Incubation: 37Β°C, Assay: 37Β°C | emulsion : 70% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent |
| Activity + Stability - Incubation | Activity after incubation (2 hours at 37Β°C) measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | Cyclohexane | 25% | 100.0 | 85.1 | % | Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2 | Incubation: 8, Assay: 8 | Incubation: 37Β°C, Assay: 37Β°C | emulsion : 70% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent |
| Activity + Stability - Incubation | Activity after incubation (2 hours at 37Β°C) measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 25% | 100.0 | 96.4 | % | Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2 | Incubation: 8, Assay: 8 | Incubation: 37Β°C, Assay: 37Β°C | emulsion : 70% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent |
| Activity + Stability - Incubation | Activity after incubation (2 hours at 37Β°C) measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | n-Hexane | 25% | 100.0 | 94.5 | % | Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2 | Incubation: 8, Assay: 8 | Incubation: 37Β°C, Assay: 37Β°C | emulsion : 70% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent |
| Activity + Stability - Incubation | Activity after incubation (2 hours at 37Β°C) measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | Acetone | 25% | 100.0 | 82.2 | % | Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2 | Incubation: 8, Assay: 8 | Incubation: 37Β°C, Assay: 37Β°C | emulsion : 70% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent |
| Activity + Stability - Incubation | Activity after incubation (2 hours at 37Β°C) measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | Diethyl Ether | 25% | 100.0 | 103.6 | % | Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2 | Incubation: 8, Assay: 8 | Incubation: 37Β°C, Assay: 37Β°C | emulsion : 70% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 37Β°C) measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | Benzene | 25% | 100.0 | 108.0 | % | Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2 | Incubation: 8, Assay: 8 | Incubation: 37Β°C, Assay: 37Β°C | emulsion : 70% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 37Β°C) measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | Isopropanol | 25% | 100.0 | 60.8 | % | Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2 | Incubation: 8, Assay: 8 | Incubation: 37Β°C, Assay: 37Β°C | emulsion : 70% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 37Β°C) measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | Toluene | 25% | 100.0 | 68.4 | % | Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2 | Incubation: 8, Assay: 8 | Incubation: 37Β°C, Assay: 37Β°C | emulsion : 70% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 37Β°C) measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | Acetonitrile | 25% | 100.0 | 69.6 | % | Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2 | Incubation: 8, Assay: 8 | Incubation: 37Β°C, Assay: 37Β°C | emulsion : 70% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 37Β°C) measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | Chloroform | 25% | 100.0 | 80.0 | % | Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2 | Incubation: 8, Assay: 8 | Incubation: 37Β°C, Assay: 37Β°C | emulsion : 70% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 37Β°C) measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 25% | 100.0 | 80.4 | % | Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2 | Incubation: 8, Assay: 8 | Incubation: 37Β°C, Assay: 37Β°C | emulsion : 70% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 37Β°C) measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | Cyclohexane | 25% | 100.0 | 84.4 | % | Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2 | Incubation: 8, Assay: 8 | Incubation: 37Β°C, Assay: 37Β°C | emulsion : 70% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 37Β°C) measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 25% | 100.0 | 85.2 | % | Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2 | Incubation: 8, Assay: 8 | Incubation: 37Β°C, Assay: 37Β°C | emulsion : 70% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 37Β°C) measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | n-Hexane | 25% | 100.0 | 94.0 | % | Incubation: Supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, Assay: supernatant of 50 mM NaH2PO4 [pH 8], 300 mM NaCl, 0.4 MM CaCl2 | Incubation: 8, Assay: 8 | Incubation: 37Β°C, Assay: 37Β°C | emulsion : 70% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent |
Visualization : Activity + Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.