Liver alcohol dehydrogenase from Equus caballus (horse)
Enzyme Description
Sequence
MSTAGKVIKCKAAVLWEEKKPFSIEEVEVAPPKAHEVRIKMVATGICRSDDHVVSGTLVTPLPVIAGHEAAGIVESIGEGVTTVRPGDKVIPLFTPQCGKCRVCKHPEGNFCLKNDLSMPRGTMQDGTSRFTCRGKPIHHFLGTSTFSQYTVVDEISVAKIDAASPLEKVCLIGCGFSTGYGSAVKVAKVTQGSTCAVFGLGGVGLSVIMGCKAAGAARIIGVDINKDKFAKAKEVGATECVNPQDYKKPIQEVLTEMSNGGVDFSFEVIGRLDTMVTALSCCQEAYGVSVIVGVPPDSQNLSMNPMLLLSGRTWKGAIFGGFKSKDSVPKLVADFMAKKFALDPLITHVLPFEKINEGFDLLRSGESIRTILTF
Annika Johansson et al. (1995)
β Horse Liver Alcohol Dehydrogenase can Accept NADP+ as Coenzyme in High Concentrations of Acetonitrile
European Journal of Biochemistry
Β· doi:10.1111/j.1432-1033.1995.tb20423.x β
Β· Activity - Classical
▼
Database ID
UniProt: P00327 β
Sequence Annotation
Inferred - from protein name
Protein Source
Commercially purchased
Experimental Data (8 measurements)
8 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity (in units of nmol*min^-1*mg*enzyme^-1) measured by HPLC in the presence of organic solvent | Acetonitrile | 98% | 1200.0 | 1.0 | nmol/(min*mg) | 10 mM Tris-HCl buffer, 10 mM octanal (cofactor recycling) | 7.4 | Room temperature | 10 mM Cinnamoyl alcohol | Cinnamaldehyde | NAD+ | β | Classical aqueous control (in nmol/(min*mg)) |
| Activity - Classical | Activity (in units of nmol*min^-1*mg*enzyme^-1) measured by HPLC in the presence of organic solvent | Acetonitrile | 95% | 1200.0 | 1.6 | nmol/(min*mg) | 10 mM Tris-HCl buffer, 10 mM octanal (cofactor recycling) | 7.4 | Room temperature | 10 mM Cinnamoyl alcohol | Cinnamaldehyde | NAD+ | β | Classical aqueous control (in nmol/(min*mg)) |
| Activity - Classical | Activity (in units of nmol*min^-1*mg*enzyme^-1) measured by HPLC in the presence of organic solvent | Acetonitrile | 90% | 1200.0 | 8.6 | nmol/(min*mg) | 10 mM Tris-HCl buffer, 10 mM octanal (cofactor recycling) | 7.4 | Room temperature | 10 mM Cinnamoyl alcohol | Cinnamaldehyde | NAD+ | β | Classical aqueous control (in nmol/(min*mg)) |
| Activity - Classical | Activity (in units of nmol*min^-1*mg*enzyme^-1) measured by HPLC in the presence of organic solvent | Acetonitrile | 75% | 1200.0 | 18.3 | nmol/(min*mg) | 10 mM Tris-HCl buffer, 10 mM octanal (cofactor recycling) | 7.4 | Room temperature | 10 mM Cinnamoyl alcohol | Cinnamaldehyde | NAD+ | β | Classical aqueous control (in nmol/(min*mg)) |
| Activity - Classical | Activity (in units of nmol*min^-1*mg*enzyme^-1) measured by HPLC in the presence of organic solvent | Acetonitrile | 98% | 45.0 | 2.4 | nmol/(min*mg) | 10 mM Tris-HCl buffer, 10 mM octanal (cofactor recycling) | 7.4 | Room temperature | 10 mM Cinnamoyl alcohol | Cinnamaldehyde | NADP+ | β | Classical aqueous control (in nmol/(min*mg)) |
| Activity - Classical | Activity (in units of nmol*min^-1*mg*enzyme^-1) measured by HPLC in the presence of organic solvent | Acetonitrile | 95% | 45.0 | 6.8 | nmol/(min*mg) | 10 mM Tris-HCl buffer, 10 mM octanal (cofactor recycling) | 7.4 | Room temperature | 10 mM Cinnamoyl alcohol | Cinnamaldehyde | NADP+ | β | Classical aqueous control (in nmol/(min*mg)) |
| Activity - Classical | Activity (in units of nmol*min^-1*mg*enzyme^-1) measured by HPLC in the presence of organic solvent | Acetonitrile | 90% | 45.0 | 11.8 | nmol/(min*mg) | 10 mM Tris-HCl buffer, 10 mM octanal (cofactor recycling) | 7.4 | Room temperature | 10 mM Cinnamoyl alcohol | Cinnamaldehyde | NADP+ | β | Classical aqueous control (in nmol/(min*mg)) |
| Activity - Classical | Activity (in units of nmol*min^-1*mg*enzyme^-1) measured by HPLC in the presence of organic solvent | Acetonitrile | 75% | 45.0 | 1.1 | nmol/(min*mg) | 10 mM Tris-HCl buffer, 10 mM octanal (cofactor recycling) | 7.4 | Room temperature | 10 mM Cinnamoyl alcohol | Cinnamaldehyde | NADP+ | β | Classical aqueous control (in nmol/(min*mg)) |
Visualization : Activity β Classical
Annika Johansson et al. (1995)
One bar per measurement. Colour = solvent, shade = solvent volume.
Daria Giacomini et al. (2007)
β Highly efficient asymmetric reduction of arylpropionic aldehydes by Horse Liver Alcohol Dehydrogenase through dynamic kinetic resolution
Chemical Communications
Β· doi:10.1039/b712290j β
Β· Activity + Stability - Product Formation
▼
Experimental Data (7 measurements)
7 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity + Stability - Product Formation | Total yield in alcohol product after incubation (5 hours at room temperature) in the presence of organic solvent, measured by HPLC | Tetrahydrofuran (THF) | 10% | 48.8 | 36.6 | % | 0.1 M phosphate buffer, 0.5 M Ethanol | 7.5 | Room temperature | 0.5 mM Ibuprofenal | Ibuprofen Alcohol | 0.01 mM NADH | β | Classical aqueous control (in %) |
| Activity + Stability - Product Formation | Total yield in alcohol product after incubation (5 hours at room temperature) in the presence of organic solvent, measured by HPLC | Acetonitrile | 10% | 48.8 | 81.4 | % | 0.1 M phosphate buffer, 0.5 M Ethanol | 7.5 | Room temperature | 0.5 mM Ibuprofenal | Ibuprofen Alcohol | 0.01 mM NADH | β | Classical aqueous control (in %) |
| Activity + Stability - Product Formation | Total yield in alcohol product after incubation (24 hours at room temperature) in the presence of organic solvent, measured by HPLC | Tetrahydrofuran (THF) | 10% | 69.3 | 45.6 | % | 0.1 M phosphate buffer, 0.5 M Ethanol | 7.5 | Room temperature | 0.5 mM Ibuprofenal | Ibuprofen Alcohol | 0.01 mM NADH | β | Classical aqueous control (in %) |
| Activity + Stability - Product Formation | Total yield in alcohol product after incubation (24 hours at room temperature) in the presence of organic solvent, measured by HPLC | Acetonitrile | 10% | 69.3 | 93.0 | % | 0.1 M phosphate buffer, 0.5 M Ethanol | 7.5 | Room temperature | 0.5 mM Ibuprofenal | Ibuprofen Alcohol | 0.01 mM NADH | β | Classical aqueous control (in %) |
| Activity + Stability - Product Formation | Total yield in alcohol product after incubation (5 hours at room temperature) in the presence of organic solvent, measured by HPLC | Acetonitrile | 10% | 81.1 | 71.9 | % | 0.1 M phosphate buffer, 0.5 M Ethanol | 7.5 | Room temperature | 0.5 mM 2-Phenylpropanal | 2-Phenylpropanol | 1 mM NADH | β | Classical aqueous control (in %) |
| Activity + Stability - Product Formation | Total yield in alcohol product after incubation (5 hours at room temperature) in the presence of organic solvent, measured by HPLC | Tetrahydrofuran (THF) | 10% | 81.1 | 54.7 | % | 0.1 M phosphate buffer, 0.5 M Ethanol | 7.5 | Room temperature | 0.5 mM 2-Phenylpropanal | 2-Phenylpropanol | 1 mM NADH | β | Classical aqueous control (in %) |
| Activity + Stability - Product Formation | Total yield in alcohol product after incubation (5 hours at room temperature) in the presence of organic solvent, measured by HPLC | Tetrahydrofuran (THF) | 10% | 88.7 | 54.1 | % | 0.1 M phosphate buffer, 0.5 M Ethanol | 7.5 | Room temperature | 0.5 mM 2-Phenylpropanal | 2-Phenylpropanol | 0.01 mM NADH | β | Classical aqueous control (in %) |
Visualization : Activity + Stability β Product Formation
Daria Giacomini et al. (2007)
One bar per measurement. Colour = solvent, shade = solvent volume.
Leanne M. Timpson et al. (2011)
β Characterization of alcohol dehydrogenase (ADH12) from Haloarcula marismortui, an extreme halophile from the Dead Sea
Extremophiles
Β· doi:10.1007/s00792-011-0405-0 β
Β· Stability - Incubation
▼
Database ID
UniProt: P00327 β
Sequence Annotation
Inferred - from protein name
Protein Source
Commercially purchased
Experimental Data (2 measurements)
2 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Tetrahydrofuran (THF) | 10% | 100 | 0 | % | Incubation: ?, Assay: 100 mM sodium pyrophosphate buffer | Incubation: ?, Assay: 8.8 | Incubation: 4Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NAD+ | β | Non-incubated control (in %), control and residual activity measured in the presence of organic solvent | High uncertainty about whether activity measured with or without organic solvent, as well as for control |
| Stability - Incubation | Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Acetonitrile | 10% | 100 | 73 | % | Incubation: ?, Assay: 100 mM sodium pyrophosphate buffer | Incubation: ?, Assay: 8.8 | Incubation: 4Β°C, Assay: 50Β°C | 100 mM Ethanol | Acetaldehyde | 1 mM NAD+ | β | Non-incubated control (in %), control and residual activity measured in the presence of organic solvent | High uncertainty about whether activity measured with or without organic solvent, as well as for control |
Visualization : Stability β Incubation
Leanne M. Timpson et al. (2011)
One bar per measurement. Colour = solvent, shade = solvent volume.
Leanne M. Timpson et al. (2012)
β A comparison of two novel alcohol dehydrogenase enzymes (ADH1 and ADH2) from the extreme halophile Haloferax volcanii
Applied Microbiology and Biotechnology
Β· doi:10.1007/s00253-012-4074-4 β
Β· Stability - Incubation
▼
Database ID
UniProt: P00327 β
Sequence Annotation
Inferred - from protein name
Protein Source
Commercially purchased
Experimental Data (5 measurements)
5 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) | Dimethyl Sulfoxide (DMSO) | 10% | 100 | 10 | % | Incubation: 100 mM Tris-HCl, 2M KCl, Assay: 100 mM pyrophosphate buffer | Incubation: 8, Assay: 8.8 | Incubation: 4Β°C, Assay: ? | 100 mM Ethanol | Acetaldehyde | 1 mM NAD+ | β | Non-incubated control (in %), control and assay in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) | Dimethyl Sulfoxide (DMSO) | 20% | 100 | 7 | % | Incubation: 100 mM Tris-HCl, 2M KCl, Assay: 100 mM pyrophosphate buffer | Incubation: 8, Assay: 8.8 | Incubation: 4Β°C, Assay: ? | 100 mM Ethanol | Acetaldehyde | 1 mM NAD+ | β | Non-incubated control (in %), control and assay in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) | Acetonitrile | 10% | 100 | 73 | % | Incubation: 100 mM Tris-HCl, 2M KCl, Assay: 100 mM pyrophosphate buffer | Incubation: 8, Assay: 8.8 | Incubation: 4Β°C, Assay: ? | 100 mM Ethanol | Acetaldehyde | 1 mM NAD+ | β | Non-incubated control (in %), control and assay in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) | Acetonitrile | 20% | 100 | 0 | % | Incubation: 100 mM Tris-HCl, 2M KCl, Assay: 100 mM pyrophosphate buffer | Incubation: 8, Assay: 8.8 | Incubation: 4Β°C, Assay: ? | 100 mM Ethanol | Acetaldehyde | 1 mM NAD+ | β | Non-incubated control (in %), control and assay in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Tetrahydrofuran (THF) | 10% | 100 | 0 | % | Incubation: 100 mM Tris-HCl, 2M KCl, Assay: 100 mM pyrophosphate buffer | Incubation: 8, Assay: 8.8 | Incubation: 4Β°C, Assay: ? | 100 mM Ethanol | Acetaldehyde | 1 mM NAD+ | β | Non-incubated control (in %), control and assay in the presence of organic solvent | High uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control |
Visualization : Stability β Incubation
Leanne M. Timpson et al. (2012)
One bar per measurement. Colour = solvent, shade = solvent volume.
Ningning Zhang et al. (2022)
β Unraveling Alcohol Dehydrogenase Catalysis in OrganicβAqueous Biphasic Systems Combining Experiments and Molecular Dynamics Simulations
ACS Catalysis
Β· doi:10.1021/acscatal.2c02881 β
Β· Activity - Classical Activity + Stability - Product Formation Specificity - Enantioselectivity Stability - Half-life
▼
Database ID
UniProt: P00327 β
Sequence Annotation
Explicit - Provided PDB Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (35 measurements)
35 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by gas chromatography in the presence of organic solvent | 2-Methyltetrahydrofuran (Me-THF) | 50% | 100.0 | 2.1 | % | 50 mM TrisβHCl buffer, 100 mM 1.4-butanediol | 7.5 | 25 | 100 mM Cyclohexanone | Cyclohexanol | 1 mM NAD+ | 1200 rpm | Classical aqueous control (in %) | 1,4-butanediol added to recycle the NAD+/NADH cofactor |
| Activity - Classical | Activity measured by gas chromatography in the presence of organic solvent | tert-Butyl Methyl Ether (MTBE) | 50% | 100.0 | 2.8 | % | 50 mM TrisβHCl buffer, 100 mM 1.4-butanediol | 7.5 | 25 | 100 mM Cyclohexanone | Cyclohexanol | 1 mM NAD+ | 1200 rpm | Classical aqueous control (in %) | 1,4-butanediol added to recycle the NAD+/NADH cofactor |
| Activity - Classical | Activity measured by gas chromatography in the presence of organic solvent | Cyclopentyl Methyl Ether (CPME) | 50% | 100.0 | 6.8 | % | 50 mM TrisβHCl buffer, 100 mM 1.4-butanediol | 7.5 | 25 | 100 mM Cyclohexanone | Cyclohexanol | 1 mM NAD+ | 1200 rpm | Classical aqueous control (in %) | 1,4-butanediol added to recycle the NAD+/NADH cofactor |
| Activity - Classical | Activity measured by gas chromatography in the presence of organic solvent | Toluene | 50% | 100.0 | 0.8 | % | 50 mM TrisβHCl buffer, 100 mM 1.4-butanediol | 7.5 | 25 | 100 mM Cyclohexanone | Cyclohexanol | 1 mM NAD+ | 1200 rpm | Classical aqueous control (in %) | 1,4-butanediol added to recycle the NAD+/NADH cofactor |
| Activity - Classical | Activity measured by gas chromatography in the presence of organic solvent | Cyclohexane | 50% | 100.0 | 21.1 | % | 50 mM TrisβHCl buffer, 100 mM 1.4-butanediol | 7.5 | 25 | 100 mM Cyclohexanone | Cyclohexanol | 1 mM NAD+ | 1200 rpm | Classical aqueous control (in %) | 1,4-butanediol added to recycle the NAD+/NADH cofactor |
| Activity - Classical | Activity measured by gas chromatography in the presence of organic solvent | n-Heptane | 50% | 100.0 | 45.8 | % | 50 mM TrisβHCl buffer, 100 mM 1.4-butanediol | 7.5 | 25 | 100 mM Cyclohexanone | Cyclohexanol | 1 mM NAD+ | 1200 rpm | Classical aqueous control (in %) | 1,4-butanediol added to recycle the NAD+/NADH cofactor |
| Activity - Classical | Activity measured by gas chromatography in the presence of organic solvent | n-Dodecane | 50% | 100.0 | 35.2 | % | 50 mM TrisβHCl buffer, 100 mM 1.4-butanediol | 7.5 | 25 | 100 mM Cyclohexanone | Cyclohexanol | 1 mM NAD+ | 1200 rpm | Classical aqueous control (in %) | 1,4-butanediol added to recycle the NAD+/NADH cofactor |
| Activity - Classical | Activity measured by gas chromatography in the presence of organic solvent | Ethyl Acetate | 50% | 100.0 | 9.3 | % | 50 mM TrisβHCl buffer, 100 mM 1.4-butanediol | 7.5 | 25 | 100 mM Cyclohexanone | Cyclohexanol | 1 mM NAD+ | 1200 rpm | Classical aqueous control (in %) | 1,4-butanediol added to recycle the NAD+/NADH cofactor |
| Activity + Stability - Product Formation | Final production of cyclohexanol after incubation (360 hours at 25Β°C) in the presence of organic solvent, measured by gas chromatography | Toluene | 50% | 73.18 | 10.07 | mM | 50 mM TrisβHCl buffer, 100 mM 1.4-butanediol | 7.5 | 25 | 100 mM, 100 mM Cyclohexanone | Cyclohexanol | 1 mM NAD+ | 1200 rpm | Classical aqueous control (in mM) | 1,4-butanediol added to recycle the NAD+/NADH cofactor |
| Activity + Stability - Product Formation | Final production of cyclohexanol after incubation (360 hours at 25Β°C) in the presence of organic solvent, measured by gas chromatography | n-Dodecane | 50% | 73.18 | 73.78 | mM | 50 mM TrisβHCl buffer, 100 mM 1.4-butanediol | 7.5 | 25 | 100 mM, 100 mM Cyclohexanone | Cyclohexanol | 1 mM NAD+ | 1200 rpm | Classical aqueous control (in mM) | 1,4-butanediol added to recycle the NAD+/NADH cofactor |
| Activity + Stability - Product Formation | Final production of cyclohexanol after incubation (360 hours at 25Β°C) in the presence of organic solvent, measured by gas chromatography | n-Heptane | 50% | 73.18 | 48.3 | mM | 50 mM TrisβHCl buffer, 100 mM 1.4-butanediol | 7.5 | 25 | 100 mM, 100 mM Cyclohexanone | Cyclohexanol | 1 mM NAD+ | 1200 rpm | Classical aqueous control (in mM) | 1,4-butanediol added to recycle the NAD+/NADH cofactor |
| Activity + Stability - Product Formation | Final production of cyclohexanol after incubation (360 hours at 25Β°C) in the presence of organic solvent, measured by gas chromatography | Cyclohexane | 50% | 73.18 | 74.07 | mM | 50 mM TrisβHCl buffer, 100 mM 1.4-butanediol | 7.5 | 25 | 100 mM, 100 mM Cyclohexanone | Cyclohexanol | 1 mM NAD+ | 1200 rpm | Classical aqueous control (in mM) | 1,4-butanediol added to recycle the NAD+/NADH cofactor |
| Activity + Stability - Product Formation | Final production of cyclohexanol after incubation (360 hours at 25Β°C) in the presence of organic solvent, measured by gas chromatography | Cyclopentyl Methyl Ether (CPME) | 50% | 73.18 | 58.96 | mM | 50 mM TrisβHCl buffer, 100 mM 1.4-butanediol | 7.5 | 25 | 100 mM, 100 mM Cyclohexanone | Cyclohexanol | 1 mM NAD+ | 1200 rpm | Classical aqueous control (in mM) | 1,4-butanediol added to recycle the NAD+/NADH cofactor |
| Activity + Stability - Product Formation | Final production of cyclohexanol after incubation (360 hours at 25Β°C) in the presence of organic solvent, measured by gas chromatography | tert-Butyl Methyl Ether (MTBE) | 50% | 73.18 | 50.37 | mM | 50 mM TrisβHCl buffer, 100 mM 1.4-butanediol | 7.5 | 25 | 100 mM, 100 mM Cyclohexanone | Cyclohexanol | 1 mM NAD+ | 1200 rpm | Classical aqueous control (in mM) | 1,4-butanediol added to recycle the NAD+/NADH cofactor |
| Activity + Stability - Product Formation | Final production of cyclohexanol after incubation (360 hours at 25Β°C) in the presence of organic solvent, measured by gas chromatography | 2-Methyltetrahydrofuran (Me-THF) | 50% | 73.18 | 8.3 | mM | 50 mM TrisβHCl buffer, 100 mM 1.4-butanediol | 7.5 | 25 | 100 mM, 100 mM Cyclohexanone | Cyclohexanol | 1 mM NAD+ | 1200 rpm | Classical aqueous control (in mM) | 1,4-butanediol added to recycle the NAD+/NADH cofactor |
| Activity + Stability - Product Formation | Final production of cyclohexanol after incubation (360 hours at 25Β°C) in the presence of organic solvent, measured by gas chromatography | Ethyl Acetate | 50% | 73.18 | 10.37 | mM | 50 mM TrisβHCl buffer, 100 mM 1.4-butanediol | 7.5 | 25 | 100 mM, 100 mM Cyclohexanone | Cyclohexanol | 1 mM NAD+ | 1200 rpm | Classical aqueous control (in mM) | 1,4-butanediol added to recycle the NAD+/NADH cofactor |
| Specificity - Enantioselectivity | Enantioselectivity after incubation (48 hours at 25Β°C) in the presence of organic solvent, measured by gas chromatography | n-Dodecane | 50% | β₯99.0 | β₯99.0 | % | 50 mM TrisβHCl buffer, 25 mM 1.4-Butanediol | 7.5 | 25 | 50 mM Acetophenone | (S)-1-Phenylethanol | 2 mM NAD+ | 1200 rpm | Classical aqueous control (in %) | 1,4-butanediol added to recycle the NAD+/NADH cofactor |
| Specificity - Enantioselectivity | Enantioselectivity after incubation (48 hours at 25Β°C) in the presence of organic solvent, measured by gas chromatography | Toluene | 50% | β₯99.0 | β₯99.0 | % | 50 mM TrisβHCl buffer, 25 mM 1.4-Butanediol | 7.5 | 25 | 50 mM Acetophenone | (S)-1-Phenylethanol | 2 mM NAD+ | 1200 rpm | Classical aqueous control (in %) | 1,4-butanediol added to recycle the NAD+/NADH cofactor |
| Specificity - Enantioselectivity | Enantioselectivity after incubation (48 hours at 25Β°C) in the presence of organic solvent, measured by gas chromatography | tert-Butyl Methyl Ether (MTBE) | 50% | β₯99.0 | β₯99.0 | % | 50 mM TrisβHCl buffer, 25 mM 1.4-Butanediol | 7.5 | 25 | 50 mM Acetophenone | (S)-1-Phenylethanol | 2 mM NAD+ | 1200 rpm | Classical aqueous control (in %) | 1,4-butanediol added to recycle the NAD+/NADH cofactor |
| Specificity - Enantioselectivity | Enantioselectivity after incubation (48 hours at 25Β°C) in the presence of organic solvent, measured by gas chromatography | Ethyl Acetate | 50% | β₯99.0 | β₯99.0 | % | 50 mM TrisβHCl buffer, 25 mM 1.4-Butanediol | 7.5 | 25 | 50 mM Acetophenone | (S)-1-Phenylethanol | 2 mM NAD+ | 1200 rpm | Classical aqueous control (in %) | 1,4-butanediol added to recycle the NAD+/NADH cofactor |
Visualization : Activity β Classical
Ningning Zhang et al. (2022)
One bar per measurement. Colour = solvent, shade = solvent volume.
Visualization : Stability β Half-life
Ningning Zhang et al. (2022)
One bar per measurement. Colour = solvent, shade = solvent volume.
Visualization : Activity + Stability β Product Formation
Ningning Zhang et al. (2022)
One bar per measurement. Colour = solvent, shade = solvent volume.
Visualization : Specificity β Enantioselectivity
Ningning Zhang et al. (2022)
One bar per measurement. Colour = solvent, shade = solvent volume.