Ene-reductase from Thermoanaerobacter pseudethanolicus E39

Enzyme Description

Species

Thermoanaerobacter pseudethanolicus E39 ↗

Extremophile

Yes "thermophilic" organism

EC Number

1.6.99.1 ↗

Sequence

Length: 337 amino acids

MSILHMPLKIKDITIKNRIMMSPMCMYSASTDGMPNDWHIVHYATRAIGGVGLIMQEATAVESRGRITDHDLGIWNDEQVKELKKIVDICKANGAVMGIQLAHAGRKCNISYEDVVGPSPIKAGDRYKLPRELSVEEIKSIVKAFGEAAKRANLAGYDVVEIHAAHGYLIHEFLSPLSNKRKDEYGNSIENRARFLIEVIDEVRKNWPENKPIFVRVSADDYMEGGINIDMMVEYINMIKDKVDLIDVSSGGLLNVDINLYPGYQVKYAETIKKRCNIKTSAVGLITTQELAEEILSNERADLVALGRELLRNPYWVLHTYTSKEDWPKQYERAFKK

Björn V. Adalbjörnsson et al. (2010) — Biocatalysis with Thermostable Enzymes: Structure and Properties of a Thermophilic 'ene '-Reductase related to Old Yellow Enzyme

ChemBioChem · doi:10.1002/cbic.200900570 ↗ · Activity + Stability - Product Formation Specificity - Enantioselectivity

24 measurements ▼

Database ID

UniProt: B0KAH1 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli

Experimental Data (24 measurements)

24 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Specificity - Enantioselectivity	Enantiomeric excess (toward R form) after incubation (2 hours at 30°C in the presence of organic solvent, measured by gas chromatography	Dimethylformamide (DMF)	20%	26	93	%	50 mM potassium phosphate buffer, 2% DMF (Dimethylformamide)	7	30°C	5 mM Ketoisophorone	Levodione	6 mM NADH	130 rpm	Classical aqueous control (in %)
Specificity - Enantioselectivity	Enantiomeric excess (toward R form) after incubation (2 hours at 30°C in the presence of organic solvent, measured by gas chromatography	Dimethylformamide (DMF)	15%	26	92	%	50 mM potassium phosphate buffer, 2% DMF (Dimethylformamide)	7	30°C	5 mM Ketoisophorone	Levodione	6 mM NADH	130 rpm	Classical aqueous control (in %)
Specificity - Enantioselectivity	Enantiomeric excess (toward R form) after incubation (2 hours at 30°C in the presence of organic solvent, measured by gas chromatography	Dimethylformamide (DMF)	10%	26	92	%	50 mM potassium phosphate buffer, 2% DMF (Dimethylformamide)	7	30°C	5 mM Ketoisophorone	Levodione	6 mM NADH	130 rpm	Classical aqueous control (in %)
Specificity - Enantioselectivity	Enantiomeric excess (toward R form) after incubation (2 hours at 30°C in the presence of organic solvent, measured by gas chromatography	Dimethylformamide (DMF)	5%	26	92	%	50 mM potassium phosphate buffer, 2% DMF (Dimethylformamide)	7	30°C	5 mM Ketoisophorone	Levodione	6 mM NADH	130 rpm	Classical aqueous control (in %)

Activity + Stability - Product Formation

Yield after incubation (2 hours at 30°C) in the presence of organic solvent, determined by gas chromatography

Dimethylformamide (DMF)

10%

50 mM potassium phosphate buffer, 2% DMF (Dimethylformamide)

30°C

5 mM Ketoisophorone

Levodione

6 mM NADH

130 rpm

Classical aqueous control (in %)

Activity + Stability - Product Formation

Yield after incubation (2 hours at 30°C) in the presence of organic solvent, determined by gas chromatography

Dimethylformamide (DMF)

50 mM potassium phosphate buffer, 2% DMF (Dimethylformamide)

30°C

5 mM Ketoisophorone

Levodione

6 mM NADH

130 rpm

Classical aqueous control (in %)

Activity + Stability - Product Formation

Yield after incubation (2 hours at 30°C) in the presence of organic solvent, determined by gas chromatography

Acetonitrile

10%

50 mM potassium phosphate buffer, 2% DMF (Dimethylformamide)

30°C

5 mM Ketoisophorone

Levodione

6 mM NADH

130 rpm

Classical aqueous control (in %)

Activity + Stability - Product Formation

Yield after incubation (2 hours at 30°C) in the presence of organic solvent, determined by gas chromatography

Acetone

10%

50 mM potassium phosphate buffer, 2% DMF (Dimethylformamide)

30°C

5 mM Ketoisophorone

Levodione

6 mM NADH

130 rpm

Classical aqueous control (in %)

Activity + Stability - Product Formation

Yield after incubation (2 hours at 30°C) in the presence of organic solvent, determined by gas chromatography

1-Propanol

10%

50 mM potassium phosphate buffer, 2% DMF (Dimethylformamide)

30°C

5 mM Ketoisophorone

Levodione

6 mM NADH

130 rpm

Classical aqueous control (in %)

Activity + Stability - Product Formation

Yield after incubation (2 hours at 30°C) in the presence of organic solvent, determined by gas chromatography

Ethanol

10%

50 mM potassium phosphate buffer, 2% DMF (Dimethylformamide)

30°C

5 mM Ketoisophorone

Levodione

6 mM NADH

130 rpm

Classical aqueous control (in %)

Activity + Stability - Product Formation

Yield after incubation (2 hours at 30°C) in the presence of organic solvent, determined by gas chromatography

Methanol

10%

50 mM potassium phosphate buffer, 2% DMF (Dimethylformamide)

30°C

5 mM Ketoisophorone

Levodione

6 mM NADH

130 rpm

Classical aqueous control (in %)

Activity + Stability - Product Formation

Yield after incubation (2 hours at 30°C) in the presence of organic solvent, determined by gas chromatography

Tetrahydrofuran (THF)

10%

50 mM potassium phosphate buffer, 2% DMF (Dimethylformamide)

30°C

5 mM Ketoisophorone

Levodione

6 mM NADH

130 rpm

Classical aqueous control (in %)

Activity + Stability - Product Formation

Yield after incubation (2 hours at 30°C) in the presence of organic solvent, determined by gas chromatography

Dimethylformamide (DMF)

10%

50 mM potassium phosphate buffer, 2% DMF (Dimethylformamide)

30°C

5 mM Ketoisophorone

Levodione

6 mM NADH

130 rpm

Classical aqueous control (in %)

Activity + Stability - Product Formation

Yield after incubation (2 hours at 30°C) in the presence of organic solvent, determined by gas chromatography

Dimethylformamide (DMF)

30%

50 mM potassium phosphate buffer, 2% DMF (Dimethylformamide)

30°C

5 mM Ketoisophorone

Levodione

6 mM NADH

130 rpm

Classical aqueous control (in %)

Activity + Stability - Product Formation

Yield after incubation (2 hours at 30°C) in the presence of organic solvent, determined by gas chromatography

Dimethylformamide (DMF)

20%

50 mM potassium phosphate buffer, 2% DMF (Dimethylformamide)

30°C

5 mM Ketoisophorone

Levodione

6 mM NADH

130 rpm

Classical aqueous control (in %)

Activity + Stability - Product Formation

Yield after incubation (2 hours at 30°C) in the presence of organic solvent, determined by gas chromatography

Dimethylformamide (DMF)

15%

50 mM potassium phosphate buffer, 2% DMF (Dimethylformamide)

30°C

5 mM Ketoisophorone

Levodione

6 mM NADH

130 rpm

Classical aqueous control (in %)

Specificity - Enantioselectivity

Enantiomeric excess (toward R form) after incubation (2 hours at 30°C in the presence of organic solvent, measured by gas chromatography

Acetonitrile

10%

50 mM potassium phosphate buffer, 2% DMF (Dimethylformamide)

30°C

5 mM Ketoisophorone

Levodione

6 mM NADH

130 rpm

Classical aqueous control (in %)

Specificity - Enantioselectivity

Enantiomeric excess (toward R form) after incubation (2 hours at 30°C in the presence of organic solvent, measured by gas chromatography

Acetone

10%

50 mM potassium phosphate buffer, 2% DMF (Dimethylformamide)

30°C

5 mM Ketoisophorone

Levodione

6 mM NADH

130 rpm

Classical aqueous control (in %)

Specificity - Enantioselectivity

Enantiomeric excess (toward R form) after incubation (2 hours at 30°C in the presence of organic solvent, measured by gas chromatography

1-Propanol

10%

50 mM potassium phosphate buffer, 2% DMF (Dimethylformamide)

30°C

5 mM Ketoisophorone

Levodione

6 mM NADH

130 rpm

Classical aqueous control (in %)

Specificity - Enantioselectivity

Enantiomeric excess (toward R form) after incubation (2 hours at 30°C in the presence of organic solvent, measured by gas chromatography

Ethanol

10%

50 mM potassium phosphate buffer, 2% DMF (Dimethylformamide)

30°C

5 mM Ketoisophorone

Levodione

6 mM NADH

130 rpm

Classical aqueous control (in %)

Specificity - Enantioselectivity

Enantiomeric excess (toward R form) after incubation (2 hours at 30°C in the presence of organic solvent, measured by gas chromatography

Methanol

10%

50 mM potassium phosphate buffer, 2% DMF (Dimethylformamide)

30°C

5 mM Ketoisophorone

Levodione

6 mM NADH

130 rpm

Classical aqueous control (in %)

Specificity - Enantioselectivity

Enantiomeric excess (toward R form) after incubation (2 hours at 30°C in the presence of organic solvent, measured by gas chromatography

Tetrahydrofuran (THF)

10%

50 mM potassium phosphate buffer, 2% DMF (Dimethylformamide)

30°C

5 mM Ketoisophorone

Levodione

6 mM NADH

130 rpm

Classical aqueous control (in %)

Specificity - Enantioselectivity

Enantiomeric excess (toward R form) after incubation (2 hours at 30°C in the presence of organic solvent, measured by gas chromatography

Dimethylformamide (DMF)

10%

50 mM potassium phosphate buffer, 2% DMF (Dimethylformamide)

30°C

5 mM Ketoisophorone

Levodione

6 mM NADH

130 rpm

Classical aqueous control (in %)

Specificity - Enantioselectivity

Enantiomeric excess (toward R form) after incubation (2 hours at 30°C in the presence of organic solvent, measured by gas chromatography

Dimethylformamide (DMF)

30%

50 mM potassium phosphate buffer, 2% DMF (Dimethylformamide)

30°C

5 mM Ketoisophorone

Levodione

6 mM NADH

130 rpm

Classical aqueous control (in %)

Specificity - Enantioselectivity

Enantiomeric excess (toward R form) after incubation (2 hours at 30°C in the presence of organic solvent, measured by gas chromatography

Dimethylformamide (DMF)

20%

50 mM potassium phosphate buffer, 2% DMF (Dimethylformamide)

30°C

5 mM Ketoisophorone

Levodione

6 mM NADH

130 rpm

Classical aqueous control (in %)

Specificity - Enantioselectivity

Enantiomeric excess (toward R form) after incubation (2 hours at 30°C in the presence of organic solvent, measured by gas chromatography

Dimethylformamide (DMF)

15%

50 mM potassium phosphate buffer, 2% DMF (Dimethylformamide)

30°C

5 mM Ketoisophorone

Levodione

6 mM NADH

130 rpm

Classical aqueous control (in %)

Specificity - Enantioselectivity

Enantiomeric excess (toward R form) after incubation (2 hours at 30°C in the presence of organic solvent, measured by gas chromatography

Dimethylformamide (DMF)

10%

50 mM potassium phosphate buffer, 2% DMF (Dimethylformamide)

30°C

5 mM Ketoisophorone

Levodione

6 mM NADH

130 rpm

Classical aqueous control (in %)

Specificity - Enantioselectivity

Enantiomeric excess (toward R form) after incubation (2 hours at 30°C in the presence of organic solvent, measured by gas chromatography

Dimethylformamide (DMF)

50 mM potassium phosphate buffer, 2% DMF (Dimethylformamide)

30°C

5 mM Ketoisophorone

Levodione

6 mM NADH

130 rpm

Classical aqueous control (in %)

‹ Prev

1 2

Next ›

Visualization : Activity + Stability — Product Formation

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Specificity — Enantioselectivity

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

Drag to rotate · Scroll to zoom · Right-click drag to pan · Powered by Mol*

Ene-reductase from Thermoanaerobacter pseudethanolicus E39

Enzyme Description

Sequence

Experimental Data (24 measurements)

Visualization : Activity + Stability — Product Formation

Visualization : Specificity — Enantioselectivity

Structure

Loading…