Tyrosinase from Bacillus megaterium

Enzyme Description

Species

Extremophile

EC Number

1.14.18.1 ↗

Sequence

Length: 297 amino acids

MSNKYRVRKNVLHLTDTEKRDFVRTVLILKEKGIYDRYIAWHGAAGKFHTPPGSDRNAAHMSSAFLPWHREYLLRFERDLQSINPEVTLPYWEWETDAQMQDPSQSQIWSADFMGGNGNPIKDFIVDTGPFAAGRWTTIDEQGNPSGGLKRNFGATKEAPTLPTRDDVLNALKITQYDTPPWDMTSQNSFRNQLEGFINGPQLHNRVHRWVGGQMGVVPTAPNDPVFFLHHANVDRIWAVWQIIHRNQNYQPMKNGPFGQNFRDPMYPWNTTPEDVMNHRKLGYVYDIELRKSKRSS

Vered Shuster et al. (2009) — Isolation, Cloning and Characterization of a Tyrosinase with Improved Activity in Organic Solvents from Bacillus megaterium ;

Microbial Physiology · doi:10.1159/000233506 ↗ · Activity - Classical Activity - Michaelis-Menten Stability - Incubation Specificity - Reaction Specificity

66 measurements ▼

Database ID

UniProt: B2ZB02 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (66 measurements)

66 measurements — page 2 of 4

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	60%	100.0	0.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	50%	100.0	0.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	100.0	109.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100.0	170.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100.0	158.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100.0	136.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Acetone	70%	100.0	47.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Acetone	60%	100.0	66.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Ethanol	20%	100.0	136.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Ethanol	30%	100.0	155.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Ethanol	40%	100.0	111.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Ethanol	50%	100.0	113.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Ethanol	60%	100.0	116.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Ethanol	70%	100.0	96.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Ethanol	80%	100.0	88.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Methanol	10%	100.0	120.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Michaelis-Menten	Vmax (µmol/(mg*min) measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	17.2	36.2	µmol/(mg * min)	50 mM potassium phosphate buffer	7	25°C	L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in micromol/(mg * min))
Activity - Michaelis-Menten	kcat/Km (1/(mM*s)) measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	5100.0	7100.0	(1/(mM * s)	50 mM potassium phosphate buffer	7	25°C	D-DOPA (D-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in (1/(mM * s))
Activity - Michaelis-Menten	kcat (/s) measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	6.0	12.12	1/s	50 mM potassium phosphate buffer	7	25°C	D-DOPA (D-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in 1/s)
Activity - Michaelis-Menten	Vmax (µmol/(mg*min) measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	10.2	20.6	µmol/(mg * min)	50 mM potassium phosphate buffer	7	25°C	D-DOPA (D-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in micromol/(mg * min))

Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Isopropanol	40%	100.0	140.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Methanol	30%	100.0	139.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Methanol	40%	100.0	129.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Methanol	50%	100.0	102.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Methanol	60%	100.0	105.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Methanol	70%	100.0	98.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Methanol	80%	100.0	55.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Isopropanol	10%	100.0	115.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Isopropanol	20%	100.0	124.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Isopropanol	30%	100.0	152.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Methanol	20%	100.0	129.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Isopropanol	50%	100.0	123.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Isopropanol	60%	100.0	57.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Isopropanol	70%	100.0	52.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Acetone	10%	100.0	118.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Acetone	20%	100.0	113.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Acetone	30%	100.0	94.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Acetone	40%	100.0	82.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Acetone	50%	100.0	81.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Ethanol	10%	100.0	122.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	60%	100.0	0.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	50%	100.0	0.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	100.0	109.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100.0	170.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100.0	158.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100.0	136.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Acetone	70%	100.0	47.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Acetone	60%	100.0	66.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Ethanol	20%	100.0	136.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Ethanol	30%	100.0	155.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Ethanol	40%	100.0	111.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Ethanol	50%	100.0	113.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Ethanol	60%	100.0	116.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Ethanol	70%	100.0	96.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Ethanol	80%	100.0	88.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Methanol	10%	100.0	120.0	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Michaelis-Menten	Vmax (µmol/(mg*min) measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	17.2	36.2	µmol/(mg * min)	50 mM potassium phosphate buffer	7	25°C	L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in µmol/(mg * min))
Activity - Michaelis-Menten	kcat/Km (1/(mM*s)) measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	5100.0	7100.0	(1/(mM * s)	50 mM potassium phosphate buffer	7	25°C	D-DOPA (D-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in (1/(mM * s))
Activity - Michaelis-Menten	kcat (/s) measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	6.0	12.12	1/s	50 mM potassium phosphate buffer	7	25°C	D-DOPA (D-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in 1/s)
Activity - Michaelis-Menten	Vmax (µmol/(mg*min) measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	10.2	20.6	µmol/(mg * min)	50 mM potassium phosphate buffer	7	25°C	D-DOPA (D-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in µmol/(mg * min))
Activity - Michaelis-Menten	Km (mM) measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	1.18	1.7	mM	50 mM potassium phosphate buffer	7	25°C	D-DOPA (D-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in mM)
Activity - Michaelis-Menten	kcat/Km (1/(mM*s)) measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	28900.0	29200.0	1/(mM * s)	50 mM potassium phosphate buffer	7	25°C	L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in 1/(mM * s))
Activity - Michaelis-Menten	kcat (/s) measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	10.1	21.3	1/s	50 mM potassium phosphate buffer	7	25°C	L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in 1/s)
Activity - Michaelis-Menten	Km (mM) measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	0.35	0.73	mM	50 mM potassium phosphate buffer	7	25°C	L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in mM)
Specificity - Reaction Specificity	(kcat/kM)L/(kcat/kM)D measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	5.7	4.1	ratio (kcat/kM)L/(kcat/kM	50 mM potassium phosphate buffer	7	25°C	D-DOPA (D-3,4-Dihydroxyphenylalanine), L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in ratio (kcat/kM)L/(kcat/kM)D)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Methanol	50%	61.0	66.0	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	70%	61.0	0.0	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Methanol	60%	61.0	64.0	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Methanol	70%	61.0	52.0	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	61.0	54.0	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	61.0	59.0	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	61.0	48.0	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	61.0	43.0	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	50%	61.0	7.0	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	60%	61.0	0.0	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Methanol	40%	61.0	69.0	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Methanol	30%	61.0	62.0	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Methanol	20%	61.0	58.0	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Methanol	10%	61.0	64.0	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Ethanol	70%	61.0	7.0	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Ethanol	60%	61.0	29.0	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Ethanol	50%	61.0	60.0	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Ethanol	40%	61.0	76.0	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Ethanol	30%	61.0	83.0	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Ethanol	20%	61.0	90.0	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Ethanol	10%	61.0	74.0	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Activity — Michaelis-Menten

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Specificity — Reaction Specificity

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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Tyrosinase from Bacillus megaterium

Enzyme Description

Sequence

Experimental Data (66 measurements)

Visualization : Activity — Classical

Visualization : Activity — Michaelis-Menten

Visualization : Stability — Incubation

Visualization : Specificity — Reaction Specificity

Structure

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