β-mannosidase from Trichoderma reesei

Enzyme Description

Species

Extremophile

EC Number

3.2.1.25 ↗ (hydrolysis activity), 3.2.1.25 ↗ (transglycosylation activity)

Sequence

Length: 942 amino acids

MGCIRPFLAALGICASVAVSTTEHAGKASIHDLGLQKWTVTNEYGNITVPGKFPSQAHLDLHAAGVINDPYHGLNDFDLRWIAAQNWTYTSKPISGLNKHSDTETWLVFDGLDTYATVKFCDHIVGTPDNQFRQWFYDVSSLLASCKDDPVLSINFGSVPRIINAINASDDVQHWPASVVYPFEYPNRQWVRKEQNDFGWDWGPAFSPVGPWQPGRIVQLSKGGELYTLNTAIDIFRKGQFNNFAPDQIAPWVVNASLDFVGTLPEHASMSVVITDAKDGRSVLYSGKLEDIAQSDMTVTGSVTIDAHKPKLWWPRDMGDQQLYNITVSVSSPGSKTPLLVSSRRVGFRTILFSSGNITDAQIASGITPGNNWHFEINGHEFYAKGANLIPPDAFWPRVTSDRMNRLFDSVEAQNFNMLRVWSSGTYLPDWIYDIADERGVLLWSEFQFSDTLYPDSPDFKENVAGEITYNVRRLNHHASLACWMGGNEFENLMLPIAQGADPETYPYVLGQYEDLFIKTMFNIIAANSHSISYSPCSANNGWLEIDLDLPVPIVERYYNTTPGHIYGDTDFYNYDTAVSFDTSVYPVGRFANEFGFISMPSIQTWKQAVDPEDLSFNSTTVILRNHHYPAGGLTRNIRNSTLGQVEMTLAVERYYPTPDKTDPVANFSSWCHATQLFQADMYKSEIQFYRRGSGLPERQLGSLYWQLNDIWQAPTWAGLEYDGRWKVLPYVSRRTYEHVIASAFWNYTSNDLEIWVTSDLWEAASGQVSLTWVDLKGKPIENNAGMPKSVDFHVGAINTTRIISTNISSNLKIPDPRDAVLIIQLTAEGKLPNASNKQLTTFTHHNHFLPVWPNQAKVTDPRLKLSYNKSTKKFTVEATAGVSLYTWLTHPAGVLGFFDDNAFVLRPGEKKEIGFTLQEDTTGGKWVEGVTVESLWDLTTP

Elena V. Eneyskaya et al. (2009) — Transglycosylating and hydrolytic activities of the β-mannosidase from Trichoderma reesei

Biochimie · doi:10.1016/j.biochi.2009.03.009 ↗ · Activity - Classical Specificity - Reaction Specificity

28 measurements ▼

Database ID

UniProt: A0A024RXG4 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Purified, fungus Trichoderma reesei

Experimental Data (28 measurements)

28 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Specificity - Reaction Specificity	Transglycosylation product yield (in %, as [Transglycoslation products]/([Transglycoslation products]+[p-nitrophenol])) measured by HPLC in the presence of organic solvent	Dimethylformamide (DMF)	2%	11.0	10.1	% transglycosylation product yield	200 mM sodium phosphate buffer	6	37°C	50 mM p-Nitrophenyl β-D-mannopyranoside	D-Mannose , p-Nitrophenol	—	—	Classical aqueous control (in % transglycosylation product yield)
Specificity - Reaction Specificity	Transglycosylation product yield (in %, as [Transglycoslation products]/([Transglycoslation products]+[p-nitrophenol])) measured by HPLC in the presence of organic solvent	Acetonitrile	14%	11.0	5.4	% transglycosylation product yield	200 mM sodium phosphate buffer	6	37°C	50 mM p-Nitrophenyl β-D-mannopyranoside	D-Mannose , p-Nitrophenol	—	—	Classical aqueous control (in % transglycosylation product yield)
Specificity - Reaction Specificity	Transglycosylation product yield (in %, as [Transglycoslation products]/([Transglycoslation products]+[p-nitrophenol])) measured by HPLC in the presence of organic solvent	Acetonitrile	12%	11.0	6.2	% transglycosylation product yield	200 mM sodium phosphate buffer	6	37°C	50 mM p-Nitrophenyl β-D-mannopyranoside	D-Mannose , p-Nitrophenol	—	—	Classical aqueous control (in % transglycosylation product yield)
Specificity - Reaction Specificity	Transglycosylation product yield (in %, as [Transglycoslation products]/([Transglycoslation products]+[p-nitrophenol])) measured by HPLC in the presence of organic solvent	Acetonitrile	10%	11.0	7.0	% transglycosylation product yield	200 mM sodium phosphate buffer	6	37°C	50 mM p-Nitrophenyl β-D-mannopyranoside	D-Mannose , p-Nitrophenol	—	—	Classical aqueous control (in % transglycosylation product yield)
Specificity - Reaction Specificity	Transglycosylation product yield (in %, as [Transglycoslation products]/([Transglycoslation products]+[p-nitrophenol])) measured by HPLC in the presence of organic solvent	Acetonitrile	8%	11.0	7.7	% transglycosylation product yield	200 mM sodium phosphate buffer	6	37°C	50 mM p-Nitrophenyl β-D-mannopyranoside	D-Mannose , p-Nitrophenol	—	—	Classical aqueous control (in % transglycosylation product yield)
Specificity - Reaction Specificity	Transglycosylation product yield (in %, as [Transglycoslation products]/([Transglycoslation products]+[p-nitrophenol])) measured by HPLC in the presence of organic solvent	Acetonitrile	6%	11.0	8.5	% transglycosylation product yield	200 mM sodium phosphate buffer	6	37°C	50 mM p-Nitrophenyl β-D-mannopyranoside	D-Mannose , p-Nitrophenol	—	—	Classical aqueous control (in % transglycosylation product yield)
Specificity - Reaction Specificity	Transglycosylation product yield (in %, as [Transglycoslation products]/([Transglycoslation products]+[p-nitrophenol])) measured by HPLC in the presence of organic solvent	Acetonitrile	4%	11.0	9.2	% transglycosylation product yield	200 mM sodium phosphate buffer	6	37°C	50 mM p-Nitrophenyl β-D-mannopyranoside	D-Mannose , p-Nitrophenol	—	—	Classical aqueous control (in % transglycosylation product yield)
Specificity - Reaction Specificity	Transglycosylation product yield (in %, as [Transglycoslation products]/([Transglycoslation products]+[p-nitrophenol])) measured by HPLC in the presence of organic solvent	Acetonitrile	2%	11.0	10.0	% transglycosylation product yield	200 mM sodium phosphate buffer	6	37°C	50 mM p-Nitrophenyl β-D-mannopyranoside	D-Mannose , p-Nitrophenol	—	—	Classical aqueous control (in % transglycosylation product yield)

Activity - Classical	Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent	Acetonitrile	4%	100.0	175.0	%	200 mM sodium phosphate buffer	6	37°C	50 mM p-Nitrophenyl β-D-mannopyranoside	D-Mannose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent	Acetonitrile	2%	100.0	138.0	%	200 mM sodium phosphate buffer	6	37°C	50 mM p-Nitrophenyl β-D-mannopyranoside	D-Mannose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent	Dimethylformamide (DMF)	14%	100.0	220.0	%	200 mM sodium phosphate buffer	6	37°C	50 mM p-Nitrophenyl β-D-mannopyranoside	D-Mannose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent	Dimethylformamide (DMF)	12%	100.0	220.0	%	200 mM sodium phosphate buffer	6	37°C	50 mM p-Nitrophenyl β-D-mannopyranoside	D-Mannose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent	Dimethylformamide (DMF)	10%	100.0	220.0	%	200 mM sodium phosphate buffer	6	37°C	50 mM p-Nitrophenyl β-D-mannopyranoside	D-Mannose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent	Dimethylformamide (DMF)	8%	100.0	219.0	%	200 mM sodium phosphate buffer	6	37°C	50 mM p-Nitrophenyl β-D-mannopyranoside	D-Mannose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent	Dimethylformamide (DMF)	6%	100.0	211.0	%	200 mM sodium phosphate buffer	6	37°C	50 mM p-Nitrophenyl β-D-mannopyranoside	D-Mannose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent	Dimethylformamide (DMF)	4%	100.0	193.0	%	200 mM sodium phosphate buffer	6	37°C	50 mM p-Nitrophenyl β-D-mannopyranoside	D-Mannose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent	Dimethylformamide (DMF)	2%	100.0	157.0	%	200 mM sodium phosphate buffer	6	37°C	50 mM p-Nitrophenyl β-D-mannopyranoside	D-Mannose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent	Acetonitrile	14%	100.0	279.0	%	200 mM sodium phosphate buffer	6	37°C	50 mM p-Nitrophenyl β-D-mannopyranoside	D-Mannose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent	Acetonitrile	12%	100.0	276.0	%	200 mM sodium phosphate buffer	6	37°C	50 mM p-Nitrophenyl β-D-mannopyranoside	D-Mannose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent	Acetonitrile	10%	100.0	261.0	%	200 mM sodium phosphate buffer	6	37°C	50 mM p-Nitrophenyl β-D-mannopyranoside	D-Mannose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent	Acetonitrile	8%	100.0	236.0	%	200 mM sodium phosphate buffer	6	37°C	50 mM p-Nitrophenyl β-D-mannopyranoside	D-Mannose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent	Acetonitrile	6%	100.0	207.0	%	200 mM sodium phosphate buffer	6	37°C	50 mM p-Nitrophenyl β-D-mannopyranoside	D-Mannose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Specificity - Reaction Specificity	Transglycosylation product yield (in %, as [Transglycoslation products]/([Transglycoslation products]+[p-nitrophenol])) measured by HPLC in the presence of organic solvent	Dimethylformamide (DMF)	14%	11.0	5.8	% transglycosylation product yield	200 mM sodium phosphate buffer	6	37°C	50 mM p-Nitrophenyl β-D-mannopyranoside	D-Mannose, p-Nitrophenol	—	—	Classical aqueous control (in % transglycosylation product yield)
Specificity - Reaction Specificity	Transglycosylation product yield (in %, as [Transglycoslation products]/([Transglycoslation products]+[p-nitrophenol])) measured by HPLC in the presence of organic solvent	Dimethylformamide (DMF)	12%	11.0	6.5	% transglycosylation product yield	200 mM sodium phosphate buffer	6	37°C	50 mM p-Nitrophenyl β-D-mannopyranoside	D-Mannose, p-Nitrophenol	—	—	Classical aqueous control (in % transglycosylation product yield)
Specificity - Reaction Specificity	Transglycosylation product yield (in %, as [Transglycoslation products]/([Transglycoslation products]+[p-nitrophenol])) measured by HPLC in the presence of organic solvent	Dimethylformamide (DMF)	10%	11.0	7.3	% transglycosylation product yield	200 mM sodium phosphate buffer	6	37°C	50 mM p-Nitrophenyl β-D-mannopyranoside	D-Mannose, p-Nitrophenol	—	—	Classical aqueous control (in % transglycosylation product yield)
Specificity - Reaction Specificity	Transglycosylation product yield (in %, as [Transglycoslation products]/([Transglycoslation products]+[p-nitrophenol])) measured by HPLC in the presence of organic solvent	Dimethylformamide (DMF)	8%	11.0	8.0	% transglycosylation product yield	200 mM sodium phosphate buffer	6	37°C	50 mM p-Nitrophenyl β-D-mannopyranoside	D-Mannose, p-Nitrophenol	—	—	Classical aqueous control (in % transglycosylation product yield)
Specificity - Reaction Specificity	Transglycosylation product yield (in %, as [Transglycoslation products]/([Transglycoslation products]+[p-nitrophenol])) measured by HPLC in the presence of organic solvent	Dimethylformamide (DMF)	6%	11.0	8.7	% transglycosylation product yield	200 mM sodium phosphate buffer	6	37°C	50 mM p-Nitrophenyl β-D-mannopyranoside	D-Mannose, p-Nitrophenol	—	—	Classical aqueous control (in % transglycosylation product yield)
Specificity - Reaction Specificity	Transglycosylation product yield (in %, as [Transglycoslation products]/([Transglycoslation products]+[p-nitrophenol])) measured by HPLC in the presence of organic solvent	Dimethylformamide (DMF)	4%	11.0	9.4	% transglycosylation product yield	200 mM sodium phosphate buffer	6	37°C	50 mM p-Nitrophenyl β-D-mannopyranoside	D-Mannose, p-Nitrophenol	—	—	Classical aqueous control (in % transglycosylation product yield)
Specificity - Reaction Specificity	Transglycosylation product yield (in %, as [Transglycoslation products]/([Transglycoslation products]+[p-nitrophenol])) measured by HPLC in the presence of organic solvent	Dimethylformamide (DMF)	2%	11.0	10.1	% transglycosylation product yield	200 mM sodium phosphate buffer	6	37°C	50 mM p-Nitrophenyl β-D-mannopyranoside	D-Mannose, p-Nitrophenol	—	—	Classical aqueous control (in % transglycosylation product yield)
Specificity - Reaction Specificity	Transglycosylation product yield (in %, as [Transglycoslation products]/([Transglycoslation products]+[p-nitrophenol])) measured by HPLC in the presence of organic solvent	Acetonitrile	14%	11.0	5.4	% transglycosylation product yield	200 mM sodium phosphate buffer	6	37°C	50 mM p-Nitrophenyl β-D-mannopyranoside	D-Mannose, p-Nitrophenol	—	—	Classical aqueous control (in % transglycosylation product yield)
Specificity - Reaction Specificity	Transglycosylation product yield (in %, as [Transglycoslation products]/([Transglycoslation products]+[p-nitrophenol])) measured by HPLC in the presence of organic solvent	Acetonitrile	12%	11.0	6.2	% transglycosylation product yield	200 mM sodium phosphate buffer	6	37°C	50 mM p-Nitrophenyl β-D-mannopyranoside	D-Mannose, p-Nitrophenol	—	—	Classical aqueous control (in % transglycosylation product yield)
Specificity - Reaction Specificity	Transglycosylation product yield (in %, as [Transglycoslation products]/([Transglycoslation products]+[p-nitrophenol])) measured by HPLC in the presence of organic solvent	Acetonitrile	10%	11.0	7.0	% transglycosylation product yield	200 mM sodium phosphate buffer	6	37°C	50 mM p-Nitrophenyl β-D-mannopyranoside	D-Mannose, p-Nitrophenol	—	—	Classical aqueous control (in % transglycosylation product yield)
Specificity - Reaction Specificity	Transglycosylation product yield (in %, as [Transglycoslation products]/([Transglycoslation products]+[p-nitrophenol])) measured by HPLC in the presence of organic solvent	Acetonitrile	8%	11.0	7.7	% transglycosylation product yield	200 mM sodium phosphate buffer	6	37°C	50 mM p-Nitrophenyl β-D-mannopyranoside	D-Mannose, p-Nitrophenol	—	—	Classical aqueous control (in % transglycosylation product yield)
Specificity - Reaction Specificity	Transglycosylation product yield (in %, as [Transglycoslation products]/([Transglycoslation products]+[p-nitrophenol])) measured by HPLC in the presence of organic solvent	Acetonitrile	6%	11.0	8.5	% transglycosylation product yield	200 mM sodium phosphate buffer	6	37°C	50 mM p-Nitrophenyl β-D-mannopyranoside	D-Mannose, p-Nitrophenol	—	—	Classical aqueous control (in % transglycosylation product yield)
Specificity - Reaction Specificity	Transglycosylation product yield (in %, as [Transglycoslation products]/([Transglycoslation products]+[p-nitrophenol])) measured by HPLC in the presence of organic solvent	Acetonitrile	4%	11.0	9.2	% transglycosylation product yield	200 mM sodium phosphate buffer	6	37°C	50 mM p-Nitrophenyl β-D-mannopyranoside	D-Mannose, p-Nitrophenol	—	—	Classical aqueous control (in % transglycosylation product yield)
Specificity - Reaction Specificity	Transglycosylation product yield (in %, as [Transglycoslation products]/([Transglycoslation products]+[p-nitrophenol])) measured by HPLC in the presence of organic solvent	Acetonitrile	2%	11.0	10.0	% transglycosylation product yield	200 mM sodium phosphate buffer	6	37°C	50 mM p-Nitrophenyl β-D-mannopyranoside	D-Mannose, p-Nitrophenol	—	—	Classical aqueous control (in % transglycosylation product yield)

‹ Prev

1 2

Next ›

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Specificity — Reaction Specificity