Ξ²-mannosidase from Trichoderma reesei

Enzyme Description

Extremophile
No
EC Number
3.2.1.25 β†— (hydrolysis activity), 3.2.1.25 β†— (transglycosylation activity)

Sequence

Length: 942 amino acids
MGCIRPFLAALGICASVAVSTTEHAGKASIHDLGLQKWTVTNEYGNITVPGKFPSQAHLDLHAAGVINDPYHGLNDFDLRWIAAQNWTYTSKPISGLNKHSDTETWLVFDGLDTYATVKFCDHIVGTPDNQFRQWFYDVSSLLASCKDDPVLSINFGSVPRIINAINASDDVQHWPASVVYPFEYPNRQWVRKEQNDFGWDWGPAFSPVGPWQPGRIVQLSKGGELYTLNTAIDIFRKGQFNNFAPDQIAPWVVNASLDFVGTLPEHASMSVVITDAKDGRSVLYSGKLEDIAQSDMTVTGSVTIDAHKPKLWWPRDMGDQQLYNITVSVSSPGSKTPLLVSSRRVGFRTILFSSGNITDAQIASGITPGNNWHFEINGHEFYAKGANLIPPDAFWPRVTSDRMNRLFDSVEAQNFNMLRVWSSGTYLPDWIYDIADERGVLLWSEFQFSDTLYPDSPDFKENVAGEITYNVRRLNHHASLACWMGGNEFENLMLPIAQGADPETYPYVLGQYEDLFIKTMFNIIAANSHSISYSPCSANNGWLEIDLDLPVPIVERYYNTTPGHIYGDTDFYNYDTAVSFDTSVYPVGRFANEFGFISMPSIQTWKQAVDPEDLSFNSTTVILRNHHYPAGGLTRNIRNSTLGQVEMTLAVERYYPTPDKTDPVANFSSWCHATQLFQADMYKSEIQFYRRGSGLPERQLGSLYWQLNDIWQAPTWAGLEYDGRWKVLPYVSRRTYEHVIASAFWNYTSNDLEIWVTSDLWEAASGQVSLTWVDLKGKPIENNAGMPKSVDFHVGAINTTRIISTNISSNLKIPDPRDAVLIIQLTAEGKLPNASNKQLTTFTHHNHFLPVWPNQAKVTDPRLKLSYNKSTKKFTVEATAGVSLYTWLTHPAGVLGFFDDNAFVLRPGEKKEIGFTLQEDTTGGKWVEGVTVESLWDLTTP
Elena V. Eneyskaya et al. (2009) β€” Transglycosylating and hydrolytic activities of the Ξ²-mannosidase from Trichoderma reesei
Biochimie  Β· doi:10.1016/j.biochi.2009.03.009 β†—  Β· Activity - Classical Specificity - Reaction Specificity
28 measurements
Database ID
Sequence Annotation
Inferred - from protein name
Protein Source
Purified, fungus Trichoderma reesei

Experimental Data (28 measurements)

28 measurements β€” page 1 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Acetonitrile 4% 100.0 175.0 % 200 mM sodium phosphate buffer 6 37Β°C 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside D-Mannose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Acetonitrile 2% 100.0 138.0 % 200 mM sodium phosphate buffer 6 37Β°C 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside D-Mannose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Dimethylformamide (DMF) 14% 100.0 220.0 % 200 mM sodium phosphate buffer 6 37Β°C 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside D-Mannose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Dimethylformamide (DMF) 12% 100.0 220.0 % 200 mM sodium phosphate buffer 6 37Β°C 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside D-Mannose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Dimethylformamide (DMF) 10% 100.0 220.0 % 200 mM sodium phosphate buffer 6 37Β°C 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside D-Mannose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Dimethylformamide (DMF) 8% 100.0 219.0 % 200 mM sodium phosphate buffer 6 37Β°C 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside D-Mannose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Dimethylformamide (DMF) 6% 100.0 211.0 % 200 mM sodium phosphate buffer 6 37Β°C 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside D-Mannose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Dimethylformamide (DMF) 4% 100.0 193.0 % 200 mM sodium phosphate buffer 6 37Β°C 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside D-Mannose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Dimethylformamide (DMF) 2% 100.0 157.0 % 200 mM sodium phosphate buffer 6 37Β°C 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside D-Mannose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Acetonitrile 14% 100.0 279.0 % 200 mM sodium phosphate buffer 6 37Β°C 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside D-Mannose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Acetonitrile 12% 100.0 276.0 % 200 mM sodium phosphate buffer 6 37Β°C 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside D-Mannose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Acetonitrile 10% 100.0 261.0 % 200 mM sodium phosphate buffer 6 37Β°C 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside D-Mannose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Acetonitrile 8% 100.0 236.0 % 200 mM sodium phosphate buffer 6 37Β°C 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside D-Mannose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Acetonitrile 6% 100.0 207.0 % 200 mM sodium phosphate buffer 6 37Β°C 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside D-Mannose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Specificity - Reaction Specificity Transglycosylation product yield (in %, as [Transglycoslation products]/([Transglycoslation products]+[p-nitrophenol])) measured by HPLC in the presence of organic solvent Dimethylformamide (DMF) 14% 11.0 5.8 % transglycosylation product yield 200 mM sodium phosphate buffer 6 37Β°C 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside D-Mannose , p-Nitrophenol β€” β€” Classical aqueous control (in % transglycosylation product yield)
Specificity - Reaction Specificity Transglycosylation product yield (in %, as [Transglycoslation products]/([Transglycoslation products]+[p-nitrophenol])) measured by HPLC in the presence of organic solvent Dimethylformamide (DMF) 12% 11.0 6.5 % transglycosylation product yield 200 mM sodium phosphate buffer 6 37Β°C 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside D-Mannose , p-Nitrophenol β€” β€” Classical aqueous control (in % transglycosylation product yield)
Specificity - Reaction Specificity Transglycosylation product yield (in %, as [Transglycoslation products]/([Transglycoslation products]+[p-nitrophenol])) measured by HPLC in the presence of organic solvent Dimethylformamide (DMF) 10% 11.0 7.3 % transglycosylation product yield 200 mM sodium phosphate buffer 6 37Β°C 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside D-Mannose , p-Nitrophenol β€” β€” Classical aqueous control (in % transglycosylation product yield)
Specificity - Reaction Specificity Transglycosylation product yield (in %, as [Transglycoslation products]/([Transglycoslation products]+[p-nitrophenol])) measured by HPLC in the presence of organic solvent Dimethylformamide (DMF) 8% 11.0 8.0 % transglycosylation product yield 200 mM sodium phosphate buffer 6 37Β°C 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside D-Mannose , p-Nitrophenol β€” β€” Classical aqueous control (in % transglycosylation product yield)
Specificity - Reaction Specificity Transglycosylation product yield (in %, as [Transglycoslation products]/([Transglycoslation products]+[p-nitrophenol])) measured by HPLC in the presence of organic solvent Dimethylformamide (DMF) 6% 11.0 8.7 % transglycosylation product yield 200 mM sodium phosphate buffer 6 37Β°C 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside D-Mannose , p-Nitrophenol β€” β€” Classical aqueous control (in % transglycosylation product yield)
Specificity - Reaction Specificity Transglycosylation product yield (in %, as [Transglycoslation products]/([Transglycoslation products]+[p-nitrophenol])) measured by HPLC in the presence of organic solvent Dimethylformamide (DMF) 4% 11.0 9.4 % transglycosylation product yield 200 mM sodium phosphate buffer 6 37Β°C 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside D-Mannose , p-Nitrophenol β€” β€” Classical aqueous control (in % transglycosylation product yield)
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Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Specificity β€” Reaction Specificity

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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