Ξ²-mannosidase from Trichoderma reesei
Enzyme Description
Species
Extremophile
No
EC Number
3.2.1.25 β (hydrolysis activity),
3.2.1.25 β (transglycosylation activity)
Sequence
MGCIRPFLAALGICASVAVSTTEHAGKASIHDLGLQKWTVTNEYGNITVPGKFPSQAHLDLHAAGVINDPYHGLNDFDLRWIAAQNWTYTSKPISGLNKHSDTETWLVFDGLDTYATVKFCDHIVGTPDNQFRQWFYDVSSLLASCKDDPVLSINFGSVPRIINAINASDDVQHWPASVVYPFEYPNRQWVRKEQNDFGWDWGPAFSPVGPWQPGRIVQLSKGGELYTLNTAIDIFRKGQFNNFAPDQIAPWVVNASLDFVGTLPEHASMSVVITDAKDGRSVLYSGKLEDIAQSDMTVTGSVTIDAHKPKLWWPRDMGDQQLYNITVSVSSPGSKTPLLVSSRRVGFRTILFSSGNITDAQIASGITPGNNWHFEINGHEFYAKGANLIPPDAFWPRVTSDRMNRLFDSVEAQNFNMLRVWSSGTYLPDWIYDIADERGVLLWSEFQFSDTLYPDSPDFKENVAGEITYNVRRLNHHASLACWMGGNEFENLMLPIAQGADPETYPYVLGQYEDLFIKTMFNIIAANSHSISYSPCSANNGWLEIDLDLPVPIVERYYNTTPGHIYGDTDFYNYDTAVSFDTSVYPVGRFANEFGFISMPSIQTWKQAVDPEDLSFNSTTVILRNHHYPAGGLTRNIRNSTLGQVEMTLAVERYYPTPDKTDPVANFSSWCHATQLFQADMYKSEIQFYRRGSGLPERQLGSLYWQLNDIWQAPTWAGLEYDGRWKVLPYVSRRTYEHVIASAFWNYTSNDLEIWVTSDLWEAASGQVSLTWVDLKGKPIENNAGMPKSVDFHVGAINTTRIISTNISSNLKIPDPRDAVLIIQLTAEGKLPNASNKQLTTFTHHNHFLPVWPNQAKVTDPRLKLSYNKSTKKFTVEATAGVSLYTWLTHPAGVLGFFDDNAFVLRPGEKKEIGFTLQEDTTGGKWVEGVTVESLWDLTTP
Elena V. Eneyskaya et al. (2009)
β Transglycosylating and hydrolytic activities of the Ξ²-mannosidase from Trichoderma reesei
Biochimie
Β· doi:10.1016/j.biochi.2009.03.009 β
Β· Activity - Classical Specificity - Reaction Specificity
▼
Database ID
UniProt: A0A024RXG4 β
Sequence Annotation
Inferred - from protein name
Protein Source
Purified, fungus Trichoderma reesei
Experimental Data (28 measurements)
28 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent | Acetonitrile | 4% | 100.0 | 175.0 | % | 200 mM sodium phosphate buffer | 6 | 37Β°C | 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside | D-Mannose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent | Acetonitrile | 2% | 100.0 | 138.0 | % | 200 mM sodium phosphate buffer | 6 | 37Β°C | 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside | D-Mannose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 14% | 100.0 | 220.0 | % | 200 mM sodium phosphate buffer | 6 | 37Β°C | 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside | D-Mannose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 12% | 100.0 | 220.0 | % | 200 mM sodium phosphate buffer | 6 | 37Β°C | 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside | D-Mannose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 10% | 100.0 | 220.0 | % | 200 mM sodium phosphate buffer | 6 | 37Β°C | 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside | D-Mannose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 8% | 100.0 | 219.0 | % | 200 mM sodium phosphate buffer | 6 | 37Β°C | 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside | D-Mannose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 6% | 100.0 | 211.0 | % | 200 mM sodium phosphate buffer | 6 | 37Β°C | 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside | D-Mannose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 4% | 100.0 | 193.0 | % | 200 mM sodium phosphate buffer | 6 | 37Β°C | 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside | D-Mannose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 2% | 100.0 | 157.0 | % | 200 mM sodium phosphate buffer | 6 | 37Β°C | 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside | D-Mannose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent | Acetonitrile | 14% | 100.0 | 279.0 | % | 200 mM sodium phosphate buffer | 6 | 37Β°C | 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside | D-Mannose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent | Acetonitrile | 12% | 100.0 | 276.0 | % | 200 mM sodium phosphate buffer | 6 | 37Β°C | 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside | D-Mannose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent | Acetonitrile | 10% | 100.0 | 261.0 | % | 200 mM sodium phosphate buffer | 6 | 37Β°C | 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside | D-Mannose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent | Acetonitrile | 8% | 100.0 | 236.0 | % | 200 mM sodium phosphate buffer | 6 | 37Β°C | 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside | D-Mannose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity (for hydrolysis rate) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent | Acetonitrile | 6% | 100.0 | 207.0 | % | 200 mM sodium phosphate buffer | 6 | 37Β°C | 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside | D-Mannose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Specificity - Reaction Specificity | Transglycosylation product yield (in %, as [Transglycoslation products]/([Transglycoslation products]+[p-nitrophenol])) measured by HPLC in the presence of organic solvent | Dimethylformamide (DMF) | 14% | 11.0 | 5.8 | % transglycosylation product yield | 200 mM sodium phosphate buffer | 6 | 37Β°C | 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside | D-Mannose , p-Nitrophenol | β | β | Classical aqueous control (in % transglycosylation product yield) |
| Specificity - Reaction Specificity | Transglycosylation product yield (in %, as [Transglycoslation products]/([Transglycoslation products]+[p-nitrophenol])) measured by HPLC in the presence of organic solvent | Dimethylformamide (DMF) | 12% | 11.0 | 6.5 | % transglycosylation product yield | 200 mM sodium phosphate buffer | 6 | 37Β°C | 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside | D-Mannose , p-Nitrophenol | β | β | Classical aqueous control (in % transglycosylation product yield) |
| Specificity - Reaction Specificity | Transglycosylation product yield (in %, as [Transglycoslation products]/([Transglycoslation products]+[p-nitrophenol])) measured by HPLC in the presence of organic solvent | Dimethylformamide (DMF) | 10% | 11.0 | 7.3 | % transglycosylation product yield | 200 mM sodium phosphate buffer | 6 | 37Β°C | 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside | D-Mannose , p-Nitrophenol | β | β | Classical aqueous control (in % transglycosylation product yield) |
| Specificity - Reaction Specificity | Transglycosylation product yield (in %, as [Transglycoslation products]/([Transglycoslation products]+[p-nitrophenol])) measured by HPLC in the presence of organic solvent | Dimethylformamide (DMF) | 8% | 11.0 | 8.0 | % transglycosylation product yield | 200 mM sodium phosphate buffer | 6 | 37Β°C | 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside | D-Mannose , p-Nitrophenol | β | β | Classical aqueous control (in % transglycosylation product yield) |
| Specificity - Reaction Specificity | Transglycosylation product yield (in %, as [Transglycoslation products]/([Transglycoslation products]+[p-nitrophenol])) measured by HPLC in the presence of organic solvent | Dimethylformamide (DMF) | 6% | 11.0 | 8.7 | % transglycosylation product yield | 200 mM sodium phosphate buffer | 6 | 37Β°C | 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside | D-Mannose , p-Nitrophenol | β | β | Classical aqueous control (in % transglycosylation product yield) |
| Specificity - Reaction Specificity | Transglycosylation product yield (in %, as [Transglycoslation products]/([Transglycoslation products]+[p-nitrophenol])) measured by HPLC in the presence of organic solvent | Dimethylformamide (DMF) | 4% | 11.0 | 9.4 | % transglycosylation product yield | 200 mM sodium phosphate buffer | 6 | 37Β°C | 50 mM p-Nitrophenyl Ξ²-D-mannopyranoside | D-Mannose , p-Nitrophenol | β | β | Classical aqueous control (in % transglycosylation product yield) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.
Visualization : Specificity β Reaction Specificity
One bar per measurement. Colour = solvent, shade = solvent volume.