Ene-reductase XenA from Pseudomonas putida

Enzyme Description

Extremophile
No
EC Number

Sequence

Length: 363 amino acids
MSALFEPYTLKDVTLRNRIAIPPMCQYMAEDGLINDWHQVHYASMARGGAGLLVVEATAVAPEGRITPGCAGIWSDAHAQAFVPVVQAIKAAGSVPGIQIAHAGRKASANRPWEGDDHIGADDARGWETIAPSAIAFGAHLPNVPRAMTLDDIARVKQDFVDAARRARDAGFEWIELHFAHGYLGQSFFSEHSNKRTDAYGGSFDNRSRFLLETLAAVREVWPENLPLTARFGVLEYDGRDEQTLEESIELARRFKAGGLDLLSVSVGFTIPETNIPWGPAFMGPIAERVRREAKLPVTSAWGFGTPQLAEAALQANQLDLVSVGRAHLADPHWAYFAAKELGVEKASWTLPAPYAHWLERYR
Frieda A. Sorgenfrei et al. (2024) β€” Solvent concentration at 50% protein unfolding may reform enzyme stability ranking and process window identification
Nature Communications  Β· doi:10.1038/s41467-024-49774-0 β†—  Β· Activity - Classical Stability - Tm
60 measurements
Database ID
UniProt: Q9R9V9 β†—
Sequence Annotation
Explicit - Provided UniProt Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (60 measurements)

60 measurements β€” page 3 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Tm Melting temperature measured by ThermoFMN in the presence of organic solvent Ethanol 25% 49.0 34.0 Β°C 50 mM sodium phosphate 7.4 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C)
Stability - Tm Melting temperature measured by ThermoFMN in the presence of organic solvent Ethanol 30% 49.0 30.33 Β°C 50 mM sodium phosphate 7.4 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C)
Stability - Tm Melting temperature measured by ThermoFMN in the presence of organic solvent Methanol 5% 49.0 47.33 Β°C 50 mM sodium phosphate 7.4 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C)
Stability - Tm Melting temperature measured by ThermoFMN in the presence of organic solvent Methanol 10% 49.0 44.33 Β°C 50 mM sodium phosphate 7.4 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C)
Stability - Tm Melting temperature measured by ThermoFMN in the presence of organic solvent Methanol 15% 49.0 42.33 Β°C 50 mM sodium phosphate 7.4 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C)
Stability - Tm Melting temperature measured by ThermoFMN in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 5% 49.0 48.0 Β°C 50 mM sodium phosphate 7.4 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C)
Stability - Tm Melting temperature measured by ThermoFMN in the presence of organic solvent Methanol 25% 49.0 35.67 Β°C 50 mM sodium phosphate 7.4 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C)
Stability - Tm Melting temperature measured by ThermoFMN in the presence of organic solvent Methanol 30% 49.0 33.0 Β°C 50 mM sodium phosphate 7.4 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C)
Stability - Tm Melting temperature measured by ThermoFMN in the presence of organic solvent Isopropanol 5% 49.0 44.0 Β°C 50 mM sodium phosphate 7.4 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C)
Stability - Tm Melting temperature measured by ThermoFMN in the presence of organic solvent Isopropanol 10% 49.0 38.0 Β°C 50 mM sodium phosphate 7.4 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C)
Stability - Tm Melting temperature measured by ThermoFMN in the presence of organic solvent Isopropanol 15% 49.0 34.33 Β°C 50 mM sodium phosphate 7.4 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C)
Stability - Tm Melting temperature measured by ThermoFMN in the presence of organic solvent Isopropanol 20% 49.0 30.33 Β°C 50 mM sodium phosphate 7.4 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C)
Stability - Tm Melting temperature measured by ThermoFMN in the presence of organic solvent Isopropanol 25% 49.0 27.67 Β°C 50 mM sodium phosphate 7.4 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C)
Stability - Tm Melting temperature measured by ThermoFMN in the presence of organic solvent Isopropanol 30% 49.0 23.33 Β°C 50 mM sodium phosphate 7.4 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C)
Stability - Tm Melting temperature measured by ThermoFMN in the presence of organic solvent 1-Propanol 5% 49.0 41.67 Β°C 50 mM sodium phosphate 7.4 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C)
Stability - Tm Melting temperature measured by ThermoFMN in the presence of organic solvent 1-Propanol 10% 49.0 32.33 Β°C 50 mM sodium phosphate 7.4 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C)
Stability - Tm Melting temperature measured by ThermoFMN in the presence of organic solvent 1-Propanol 15% 49.0 29.0 Β°C 50 mM sodium phosphate 7.4 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C)
Stability - Tm Melting temperature measured by ThermoFMN in the presence of organic solvent 1-Propanol 20% 49.0 28.0 Β°C 50 mM sodium phosphate 7.4 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C)
Stability - Tm Melting temperature measured by ThermoFMN in the presence of organic solvent 1-Propanol 25% 49.0 27.33 Β°C 50 mM sodium phosphate 7.4 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C)
Stability - Tm Melting temperature measured by ThermoFMN in the presence of organic solvent 1-Propanol 30% 49.0 24.67 Β°C 50 mM sodium phosphate 7.4 β€” β€” β€” β€” β€” Classical aqueous control (in Β°C)
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Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability β€” Tm

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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