Thermolysin from Geobacillus stearothermophilus

Enzyme Description

Species
Geobacillus stearothermophilus β†—
Extremophile
Yes thermophilic organism
EC Number
3.4.24.27 β†—

Sequence

Length: 316 amino acids
ITGTSTVGVGRGVLGDQKNINTTYSTYYYLQDNTRGNGIFTYDAKYRTTLPGSLWADADNQFFASYDAPAVDAHYYAGVTYDYYKNVHNRLSYDGNNAAIRSSVHYSQGYNNAFWNGSQMVYGDGDGQTFIPLSGGIDVVAHELTHAVTDYTAGLIYQNESGAINEAISDIFGTLVEFYANKNPDWEIGEDVYTPGISGDSLRSMSDPAKYGDPDHYSKRYTGTQDNGGVHINSGIINKAAYLISQGGTHYGVSVVGIGRDKLGKIFYRALTQYLTPTSNFSQLRAAAVQSATDLYGSTSQEVASVKQAFDAVGVK
Hiroyasu Ogino et al. (1999) β€” Purification and characterization of organic solvent-stable protease from organic solvent-tolerant Pseudomonas aeruginosa PST-01
Journal of Bioscience and Bioengineering  Β· doi:10.1016/s1389-1723(99)80009-7 β†—  Β· Stability - Half-life
16 measurements ▼
Database ID
UniProt: P43133 β†—
Sequence Annotation
Inferred - from protein name (235 first residues from UniProt sequence absent from the mature protein)
Protein Source
Commercially purchased

Experimental Data (16 measurements)

16 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase Ethylene Glycol 25% 10.8 >50.0 days Incubation: 10 mM Borax-HCl buffer, Assay: 50 mM Borax-HCl buffer Incubation: 8.5, Assay: 8.5 Incubation: 30Β°C, Assay: 30Β°C 0.6% (w/v) Casein free amino acids , Peptides β€” Incubation: 160 rpm Classical aqueous control (in days) | Uncertainty about the species of thermolysin
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase 1,4-Butanediol 25% 10.8 4.4 days Incubation: 10 mM Borax-HCl buffer, Assay: 50 mM Borax-HCl buffer Incubation: 8.5, Assay: 8.5 Incubation: 30Β°C, Assay: 30Β°C 0.6% (w/v) Casein free amino acids , Peptides β€” Incubation: 160 rpm Classical aqueous control (in days) | Uncertainty about the species of thermolysin
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase 1,5-Pentanediol 25% 10.8 1.7 days Incubation: 10 mM Borax-HCl buffer, Assay: 50 mM Borax-HCl buffer Incubation: 8.5, Assay: 8.5 Incubation: 30Β°C, Assay: 30Β°C 0.6% (w/v) Casein free amino acids , Peptides β€” Incubation: 160 rpm Classical aqueous control (in days) | Uncertainty about the species of thermolysin
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase Ethanol 25% 10.8 3.0 days Incubation: 10 mM Borax-HCl buffer, Assay: 50 mM Borax-HCl buffer Incubation: 8.5, Assay: 8.5 Incubation: 30Β°C, Assay: 30Β°C 0.6% (w/v) Casein free amino acids , Peptides β€” Incubation: 160 rpm Classical aqueous control (in days) | Uncertainty about the species of thermolysin
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase 1-Hexanol 25% 10.8 18.2 days Incubation: 10 mM Borax-HCl buffer, Assay: 50 mM Borax-HCl buffer Incubation: 8.5, Assay: 8.5 Incubation: 30Β°C, Assay: 30Β°C 0.6% (w/v) Casein free amino acids , Peptides β€” Incubation: 160 rpm Classical aqueous control (in days) | Uncertainty about the species of thermolysin
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase Methanol 25% 10.8 4.6 days Incubation: 10 mM Borax-HCl buffer, Assay: 50 mM Borax-HCl buffer Incubation: 8.5, Assay: 8.5 Incubation: 30Β°C, Assay: 30Β°C 0.6% (w/v) Casein free amino acids , Peptides β€” Incubation: 160 rpm Classical aqueous control (in days) | Uncertainty about the species of thermolysin
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 25% 10.8 2.6 days Incubation: 10 mM Borax-HCl buffer, Assay: 50 mM Borax-HCl buffer Incubation: 8.5, Assay: 8.5 Incubation: 30Β°C, Assay: 30Β°C 0.6% (w/v) Casein free amino acids , Peptides β€” Incubation: 160 rpm Classical aqueous control (in days) | Uncertainty about the species of thermolysin
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase Isopropanol 25% 10.8 1.2 days Incubation: 10 mM Borax-HCl buffer, Assay: 50 mM Borax-HCl buffer Incubation: 8.5, Assay: 8.5 Incubation: 30Β°C, Assay: 30Β°C 0.6% (w/v) Casein free amino acids , Peptides β€” Incubation: 160 rpm Classical aqueous control (in days) | Uncertainty about the species of thermolysin
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase Triethylene Glycol 25% 10.8 5.1 days Incubation: 10 mM Borax-HCl buffer, Assay: 50 mM Borax-HCl buffer Incubation: 8.5, Assay: 8.5 Incubation: 30Β°C, Assay: 30Β°C 0.6% (w/v) Casein free amino acids , Peptides β€” Incubation: 160 rpm Classical aqueous control (in days) | Uncertainty about the species of thermolysin
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase tert-Butanol 25% 10.8 0.8 days Incubation: 10 mM Borax-HCl buffer, Assay: 50 mM Borax-HCl buffer Incubation: 8.5, Assay: 8.5 Incubation: 30Β°C, Assay: 30Β°C 0.6% (w/v) Casein free amino acids , Peptides β€” Incubation: 160 rpm Classical aqueous control (in days) | Uncertainty about the species of thermolysin
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase 1-Heptanol 25% 10.8 13.1 days Incubation: 10 mM Borax-HCl buffer, Assay: 50 mM Borax-HCl buffer Incubation: 8.5, Assay: 8.5 Incubation: 30Β°C, Assay: 30Β°C 0.6% (w/v) Casein free amino acids , Peptides β€” Incubation: 160 rpm Classical aqueous control (in days) | Uncertainty about the species of thermolysin
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase Dimethylformamide (DMF) 25% 10.8 0.9 days Incubation: 10 mM Borax-HCl buffer, Assay: 50 mM Borax-HCl buffer Incubation: 8.5, Assay: 8.5 Incubation: 30Β°C, Assay: 30Β°C 0.6% (w/v) Casein free amino acids , Peptides β€” Incubation: 160 rpm Classical aqueous control (in days) | Uncertainty about the species of thermolysin
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase 1-Butanol 25% 10.8 0.9 days Incubation: 10 mM Borax-HCl buffer, Assay: 50 mM Borax-HCl buffer Incubation: 8.5, Assay: 8.5 Incubation: 30Β°C, Assay: 30Β°C 0.6% (w/v) Casein free amino acids , Peptides β€” Incubation: 160 rpm Classical aqueous control (in days) | Uncertainty about the species of thermolysin
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase Acetone 25% 10.8 0.7 days Incubation: 10 mM Borax-HCl buffer, Assay: 50 mM Borax-HCl buffer Incubation: 8.5, Assay: 8.5 Incubation: 30Β°C, Assay: 30Β°C 0.6% (w/v) Casein free amino acids , Peptides β€” Incubation: 160 rpm Classical aqueous control (in days) | Uncertainty about the species of thermolysin
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase 1,4-Dioxane 25% 10.8 0.8 days Incubation: 10 mM Borax-HCl buffer, Assay: 50 mM Borax-HCl buffer Incubation: 8.5, Assay: 8.5 Incubation: 30Β°C, Assay: 30Β°C 0.6% (w/v) Casein free amino acids , Peptides β€” Incubation: 160 rpm Classical aqueous control (in days) | Uncertainty about the species of thermolysin
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase Toluene 25% 10.8 22.5 days Incubation: 10 mM Borax-HCl buffer, Assay: 50 mM Borax-HCl buffer Incubation: 8.5, Assay: 8.5 Incubation: 30Β°C, Assay: 30Β°C 0.6% (w/v) Casein free amino acids , Peptides β€” Incubation: 160 rpm Classical aqueous control (in days) | Uncertainty about the species of thermolysin

Visualization : Stability β€” Half-life

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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