Lipase (gene lipA) from Ralstonia sp. M1
Enzyme Description
Sequence
MSKPSPLRAVRGFLRTHAAAAVVAASTVTIAAFAPAQAASTYAATKYPIVLVHGLSGTDKFLGTVDYWYQIPEDLRANGATVYVANVSAFNDETVRGEQLVSQIRSVLATTGAAKVNLIGHSQGGLTSRYAAAVIPDKVASVTTIGTPHKGSEFADFVESAPDPLKALLNIGADLAGTVLSWFNGNSNPQDGYAALRILSTAGAADFNTAFPSAGLASGCNTGSSTDVRNGNVQKLYSWTGRSTATNILDLFDPALVFTGTVMQARGSGTNDGLVSVCSAKFGQVLSTDYAWNHLDEVNQLLGLIGPFAADPVAVIRTQANRLKTAGL
Dinh Thi Quyen et al. (2005)
β High-level heterologous expression and properties of a novel lipase from Ralstonia sp. M1
Protein Expression and Purification
Β· doi:10.1016/j.pep.2004.10.001 β
Β· Stability - Incubation
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Database ID
UniProt: Q67DX2 β
Sequence Annotation
Inferred - from protein name
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (26 measurements)
26 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C) measured by pH-metry (free fatty acid titration with NaOH) in aqueous phase | Methanol | 30% | 100 | 104 | % | 0.1M phosphate buffer | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 55Β°C | 1% (v/v) Olive oil | free fatty acids , glycerol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C) measured by pH-metry (free fatty acid titration with NaOH) in aqueous phase | Dimethylformamide (DMF) | 30% | 100 | 43 | % | 0.1M phosphate buffer | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 55Β°C | 1% (v/v) Olive oil | free fatty acids , glycerol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C) measured by pH-metry (free fatty acid titration with NaOH) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 30% | 100 | 100 | % | 0.1M phosphate buffer | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 55Β°C | 1% (v/v) Olive oil | free fatty acids , glycerol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C) measured by pH-metry (free fatty acid titration with NaOH) in aqueous phase | n-Hexane | 30% | 100 | 153 | % | 0.1M phosphate buffer | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 55Β°C | 1% (v/v) Olive oil | free fatty acids , glycerol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C) measured by pH-metry (free fatty acid titration with NaOH) in aqueous phase | Ethanolamine | 30% | 100 | 334 | % | 0.1M phosphate buffer | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 55Β°C | 1% (v/v) Olive oil | free fatty acids , glycerol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C) measured by pH-metry (free fatty acid titration with NaOH) in aqueous phase | Acetone | 30% | 100 | 55 | % | 0.1M phosphate buffer | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 55Β°C | 1% (v/v) Olive oil | free fatty acids , glycerol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C) measured by pH-metry (free fatty acid titration with NaOH) in aqueous phase | Acetonitrile | 30% | 100 | 40 | % | 0.1M phosphate buffer | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 55Β°C | 1% (v/v) Olive oil | free fatty acids , glycerol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C) measured by pH-metry (free fatty acid titration with NaOH) in aqueous phase | Ethyl Acetate | 30% | 100 | 198 | % | 0.1M phosphate buffer | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 55Β°C | 1% (v/v) Olive oil | free fatty acids , glycerol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C) measured by pH-metry (free fatty acid titration with NaOH) in aqueous phase | Dichloromethane | 30% | 100 | 155 | % | 0.1M phosphate buffer | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 55Β°C | 1% (v/v) Olive oil | free fatty acids , glycerol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C) measured by pH-metry (free fatty acid titration with NaOH) in aqueous phase | tert-Butanol | 30% | 100 | 141 | % | 0.1M phosphate buffer | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 55Β°C | 1% (v/v) Olive oil | free fatty acids , glycerol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C) measured by pH-metry (free fatty acid titration with NaOH) in aqueous phase | 1-Butanol | 30% | 100 | 71 | % | 0.1M phosphate buffer | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 55Β°C | 1% (v/v) Olive oil | free fatty acids , glycerol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C) measured by pH-metry (free fatty acid titration with NaOH) in aqueous phase | Isopropanol | 30% | 100 | 56 | % | 0.1M phosphate buffer | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 55Β°C | 1% (v/v) Olive oil | free fatty acids , glycerol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C) measured by pH-metry (free fatty acid titration with NaOH) in aqueous phase | Ethanol | 30% | 100 | 56 | % | 0.1M phosphate buffer | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 55Β°C | 1% (v/v) Olive oil | free fatty acids , glycerol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C) measured by pH-metry (free fatty acid titration with NaOH) in aqueous phase | Methanol | 10% | 100 | 110 | % | 0.1M phosphate buffer | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 55Β°C | 1% (v/v) Olive oil | free fatty acids , glycerol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C) measured by pH-metry (free fatty acid titration with NaOH) in aqueous phase | Dimethylformamide (DMF) | 10% | 100 | 128 | % | 0.1M phosphate buffer | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 55Β°C | 1% (v/v) Olive oil | free fatty acids , glycerol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C) measured by pH-metry (free fatty acid titration with NaOH) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 10% | 100 | 164 | % | 0.1M phosphate buffer | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 55Β°C | 1% (v/v) Olive oil | free fatty acids , glycerol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C) measured by pH-metry (free fatty acid titration with NaOH) in aqueous phase | n-Hexane | 10% | 100 | 152 | % | 0.1M phosphate buffer | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 55Β°C | 1% (v/v) Olive oil | free fatty acids , glycerol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C) measured by pH-metry (free fatty acid titration with NaOH) in aqueous phase | Ethanolamine | 10% | 100 | 137 | % | 0.1M phosphate buffer | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 55Β°C | 1% (v/v) Olive oil | free fatty acids , glycerol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C) measured by pH-metry (free fatty acid titration with NaOH) in aqueous phase | Acetone | 10% | 100 | 116 | % | 0.1M phosphate buffer | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 55Β°C | 1% (v/v) Olive oil | free fatty acids , glycerol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C) measured by pH-metry (free fatty acid titration with NaOH) in aqueous phase | Acetonitrile | 10% | 100 | 86 | % | 0.1M phosphate buffer | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 55Β°C | 1% (v/v) Olive oil | free fatty acids , glycerol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.