Esterase (gene ST0071) from Sulfolobus tokodaii strain 7

Enzyme Description

Species
Sulfolobus tokodaii strain 7 β†—
Extremophile
Yes "thermoacidophilic" organism
EC Number
3.1.1.1 β†—

Sequence

Length: 303 amino acids
MIDPKIKKLLESTIQLPIGKASVEEIRSLFKQFSSLTPREEVGKIEDITIPGSETNIKARVYYPKTQGPYGVLVYYHGGGFVLGDIESYDPLCRAITNSCQCVTISVDYRLAPENKFPAAVVDSFDALKWVYNNSEKFNGKYGIAVGGDSAGGNLAAVTAILSKKENIKLKYQVLIYPAVSFDLITKSLYDNGEGFFLTREHIDWFGQQYLRSFADLLDFRFSPILADLNDLPPALIITAEHDPLRDQGEAYANKLLQSGVQVTSVRFNNVIHGFVSFFPFIEQGRDAIGLIGYVLRKVFYGK
Yoichi Suzuki et al. (2004) β€” A novel thermostable esterase from the thermoacidophilic archaeon Sulfolobus tokodaii strain 7
FEMS Microbiology Letters  Β· doi:10.1016/j.femsle.2004.05.026 β†—  Β· Activity - Classical
12 measurements ▼
Database ID
UniProt: Q976W8 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli bL21 (DE3)

Experimental Data (12 measurements)

12 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Acetonitrile 10% 100 96 % 50 mM HEPES 7.5 70Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Acetonitrile 20% 100 75 % 50 mM HEPES 7.5 70Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Acetonitrile 30% 100 50 % 50 mM HEPES 7.5 70Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent 1,4-Dioxane 10% 100 52 % 50 mM HEPES 7.5 70Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent 1,4-Dioxane 20% 100 30 % 50 mM HEPES 7.5 70Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent 1,4-Dioxane 30% 100 13 % 50 mM HEPES 7.5 70Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10% 100 80 % 50 mM HEPES 7.5 70Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 20% 100 70 % 50 mM HEPES 7.5 70Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 30% 100 41 % 50 mM HEPES 7.5 70Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent tert-Butanol 10% 100 26 % 50 mM HEPES 7.5 70Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent tert-Butanol 20% 100 11 % 50 mM HEPES 7.5 70Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent tert-Butanol 30% 100 2 % 50 mM HEPES 7.5 70Β°C 0.5 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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