UDP-glucuronosyltransferase 1A3 from Homo sapiens

Enzyme Description

Species
Homo sapiens (human) β†—
Extremophile
No
EC Number
2.4.1.17 β†—

Sequence

Length: 534 amino acids
MATGLQVPLPWLATGLLLLLSVQPWAESGKVLVVPIDGSHWLSMREVLRELHARGHQAVVLTPEVNMHIKEENFFTLTTYAISWTQDEFDRHVLGHTQLYFETEHFLKKFFRSMAMLNNMSLVYHRSCVELLHNEALIRHLNATSFDVVLTDPVNLCAAVLAKYLSIPTVFFLRNIPCDLDFKGTQCPNPSSYIPRLLTTNSDHMTFMQRVKNMLYPLALSYICHAFSAPYASLASELFQREVSVVDILSHASVWLFRGDFVMDYPRPIMPNMVFIGGINCANRKPLSQEFEAYINASGEHGIVVFSLGSMVSEIPEKKAMAIADALGKIPQTVLWRYTGTRPSNLANNTILVKWLPQNDLLGHPMTRAFITHAGSHGVYESICNGVPMVMMPLFGDQMDNAKRMETKGAGVTLNVLEMTSEDLENALKAVINDKSYKENIMRLSSLHKDRPVEPLDLAVFWVEFVMRHKGAPHLRPAAHDLTWYQYHSLDVIGFLLAVVLTVAFITFKCCAYGYRKCLGKKGRVKKAHKSKTH
Verawan Uchaipichat et al. (2004) β€” Human udp-glucuronosyltransferases: isoform selectivity and kinetics of 4-methylumbelliferone and 1-naphthol glucuronidation, effects of organic solvents, and inhibition by diclofenac and probenecid
Drug Metabolism and Disposition  Β· doi:10.1124/dmd.32.4.413 β†—  Β· Activity - Classical
12 measurements ▼
Database ID
UniProt: P35503 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Recombinant, host human HEK293 cells

Experimental Data (12 measurements)

12 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by fluorescence spectrophotometry (4-methylumbelliferone glucuronide fluorescence measurement, excitation at 315 nm, absorbance measurement at 365 nm) in the presence of organic solvent Methanol 0.5% 100 99 % 0.1 M phosphate buffer, 5 mM MgClβ‚‚ 7.4 37Β°C 1000 Β΅M 4-Methylumbelliferyl Ξ²-D-glucuronide, 5 mM Uridine 5'-diphosphoglucuronic acid trisodium salt 4-methylumbelliferone glucuronide , UDP β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-methylumbelliferone glucuronide fluorescence measurement, excitation at 315 nm, absorbance measurement at 365 nm) in the presence of organic solvent Methanol 1% 100 93 % 0.1 M phosphate buffer, 5 mM MgClβ‚‚ 7.4 37Β°C 1000 Β΅M 4-Methylumbelliferyl Ξ²-D-glucuronide, 5 mM Uridine 5'-diphosphoglucuronic acid trisodium salt 4-methylumbelliferone glucuronide , UDP β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-methylumbelliferone glucuronide fluorescence measurement, excitation at 315 nm, absorbance measurement at 365 nm) in the presence of organic solvent Methanol 2% 100 93 % 0.1 M phosphate buffer, 5 mM MgClβ‚‚ 7.4 37Β°C 1000 Β΅M 4-Methylumbelliferyl Ξ²-D-glucuronide, 5 mM Uridine 5'-diphosphoglucuronic acid trisodium salt 4-methylumbelliferone glucuronide , UDP β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-methylumbelliferone glucuronide fluorescence measurement, excitation at 315 nm, absorbance measurement at 365 nm) in the presence of organic solvent Methanol 4% 100 88 % 0.1 M phosphate buffer, 5 mM MgClβ‚‚ 7.4 37Β°C 1000 Β΅M 4-Methylumbelliferyl Ξ²-D-glucuronide, 5 mM Uridine 5'-diphosphoglucuronic acid trisodium salt 4-methylumbelliferone glucuronide , UDP β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-methylumbelliferone glucuronide fluorescence measurement, excitation at 315 nm, absorbance measurement at 365 nm) in the presence of organic solvent Acetone 0.5% 100 96 % 0.1 M phosphate buffer, 5 mM MgClβ‚‚ 7.4 37Β°C 1000 Β΅M 4-Methylumbelliferyl Ξ²-D-glucuronide, 5 mM Uridine 5'-diphosphoglucuronic acid trisodium salt 4-methylumbelliferone glucuronide , UDP β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-methylumbelliferone glucuronide fluorescence measurement, excitation at 315 nm, absorbance measurement at 365 nm) in the presence of organic solvent Acetone 1% 100 94 % 0.1 M phosphate buffer, 5 mM MgClβ‚‚ 7.4 37Β°C 1000 Β΅M 4-Methylumbelliferyl Ξ²-D-glucuronide, 5 mM Uridine 5'-diphosphoglucuronic acid trisodium salt 4-methylumbelliferone glucuronide , UDP β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-methylumbelliferone glucuronide fluorescence measurement, excitation at 315 nm, absorbance measurement at 365 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 0.5% 100 99 % 0.1 M phosphate buffer, 5 mM MgClβ‚‚ 7.4 37Β°C 1000 Β΅M 4-Methylumbelliferyl Ξ²-D-glucuronide, 5 mM Uridine 5'-diphosphoglucuronic acid trisodium salt 4-methylumbelliferone glucuronide , UDP β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-methylumbelliferone glucuronide fluorescence measurement, excitation at 315 nm, absorbance measurement at 365 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 1% 100 94 % 0.1 M phosphate buffer, 5 mM MgClβ‚‚ 7.4 37Β°C 1000 Β΅M 4-Methylumbelliferyl Ξ²-D-glucuronide, 5 mM Uridine 5'-diphosphoglucuronic acid trisodium salt 4-methylumbelliferone glucuronide , UDP β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-methylumbelliferone glucuronide fluorescence measurement, excitation at 315 nm, absorbance measurement at 365 nm) in the presence of organic solvent Acetonitrile 0.5% 100 97 % 0.1 M phosphate buffer, 5 mM MgClβ‚‚ 7.4 37Β°C 1000 Β΅M 4-Methylumbelliferyl Ξ²-D-glucuronide, 5 mM Uridine 5'-diphosphoglucuronic acid trisodium salt 4-methylumbelliferone glucuronide , UDP β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-methylumbelliferone glucuronide fluorescence measurement, excitation at 315 nm, absorbance measurement at 365 nm) in the presence of organic solvent Acetonitrile 1% 100 94 % 0.1 M phosphate buffer, 5 mM MgClβ‚‚ 7.4 37Β°C 1000 Β΅M 4-Methylumbelliferyl Ξ²-D-glucuronide, 5 mM Uridine 5'-diphosphoglucuronic acid trisodium salt 4-methylumbelliferone glucuronide , UDP β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-methylumbelliferone glucuronide fluorescence measurement, excitation at 315 nm, absorbance measurement at 365 nm) in the presence of organic solvent Ethanol 0.5% 100 95 % 0.1 M phosphate buffer, 5 mM MgClβ‚‚ 7.4 37Β°C 1000 Β΅M 4-Methylumbelliferyl Ξ²-D-glucuronide, 5 mM Uridine 5'-diphosphoglucuronic acid trisodium salt 4-methylumbelliferone glucuronide , UDP β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by fluorescence spectrophotometry (4-methylumbelliferone glucuronide fluorescence measurement, excitation at 315 nm, absorbance measurement at 365 nm) in the presence of organic solvent Ethanol 1% 100 95 % 0.1 M phosphate buffer, 5 mM MgClβ‚‚ 7.4 37Β°C 1000 Β΅M 4-Methylumbelliferyl Ξ²-D-glucuronide, 5 mM Uridine 5'-diphosphoglucuronic acid trisodium salt 4-methylumbelliferone glucuronide , UDP β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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