Endo-β-N-acetylglucosaminidase from Mucor hiemalis

Enzyme Description

Species

Extremophile

EC Number

3.2.1.96 ↗

Sequence

Length: 744 amino acids

MPSLQLQPDDKLAPVSFALKSMNELRDWTPDEKIKFNVSSVALQPRVKNALKPQLLLTHDMAGGYKEDKNIQGNNYKDIYNIQYWHLADTFVYFSHERVSIPPVNWTNACHRNGVKCLGTFLVEGNNQMHEMEALLHGPPLLNNTDDPMRLWSPYYADQLVAIAKHYGFDGWLFNIECEFFPFPTNPKFKAEELAKFLHYFKEKLHNEIPGSQLIWYDSMTNEGEIHWQNQLTWKNELFFKNTDGIFLNYWWKKEYPEMARRVAEGIGRSGLEVYFGTDVWGRHTYGGGGFKSYKGVKTAYSAMTSSALFGMAWTYEHFEKSEFEKMDRLFWCGGKYSDYPPPPPKNPDDEKEVESDDSEDELMYGHKKGIADTVESIPVPGTDWFVTNFDRGFGNRFYYRGKRLLSQPWSHLSHQAILPNKSYRNPEIYPTDQNIKITSSLDCDHGAFLGGTSLIIKGQRFNHRESHDVETEISIPLYKLSLDASKGCSLRYIYRTLLMKDVKLTVACHFSLKTNDSVNFFKVWQPDENFSFEYDDGMRATVTTENSTESRCFLLRTTEEDTGENDWITKTINVPAVPEGSQLYITRLEVSVVLDTAGLVGLVNQVIACLGYISIIPTINSGIKTDSSRIIQDLFWKDQKYTKIGKESLDDIAQEEVHRYYGTLNWENTANVVNAWEEIDYYNVFYKESDDSATRIFLGTAFCNQFRVSGLDIILSKLPKIVIEAVNKEGYISSSGSIDLSLN

Eri Akaike et al. (2004) — High efficiency of transferring a native sugar chain from a glycopeptide by a microbial endoglycosidase in organic solvents

Carbohydrate Research · doi:10.1016/j.carres.2003.12.007 ↗ · Activity + Stability - Product Formation

12 measurements ▼

Database ID

UniProt: Q9C1S6 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Recombinant, host yeast Candida boidinii

Experimental Data (12 measurements)

12 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity + Stability - Product Formation	Product yield after incubation (3 hours at 25°C) in the presence of organic solvent, measured by HPLC	Acetone	30%	14.7	34.1	%	60 mM phosphate buffer	6.25	25°C	10 mM p-Nitrophenyl N-acetyl-β-D-glucosaminide, 20 mM Sialylglycopeptide	p-Nitrophenyl-GlcNAc-oligosaccharide	—	—	Classical aqueous control (in %)
Activity + Stability - Product Formation	Product yield after incubation (3 hours at 25°C) in the presence of organic solvent, measured by HPLC	2-Butanone	30%	14.7	0.0	%	60 mM phosphate buffer	6.25	25°C	10 mM p-Nitrophenyl N-acetyl-β-D-glucosaminide, 20 mM Sialylglycopeptide	p-Nitrophenyl-GlcNAc-oligosaccharide	—	—	Classical aqueous control (in %)
Activity + Stability - Product Formation	Product yield after incubation (3 hours at 25°C) in the presence of organic solvent, measured by HPLC	Acetonitrile	30%	14.7	0.0	%	60 mM phosphate buffer	6.25	25°C	10 mM p-Nitrophenyl N-acetyl-β-D-glucosaminide, 20 mM Sialylglycopeptide	p-Nitrophenyl-GlcNAc-oligosaccharide	—	—	Classical aqueous control (in %)
Activity + Stability - Product Formation	Product yield after incubation (3 hours at 25°C) in the presence of organic solvent, measured by HPLC	1,4-Dioxane	30%	14.7	0.0	%	60 mM phosphate buffer	6.25	25°C	10 mM p-Nitrophenyl N-acetyl-β-D-glucosaminide, 20 mM Sialylglycopeptide	p-Nitrophenyl-GlcNAc-oligosaccharide	—	—	Classical aqueous control (in %)
Activity + Stability - Product Formation	Product yield after incubation (3 hours at 25°C) in the presence of organic solvent, measured by HPLC	Dimethylformamide (DMF)	30%	14.7	0.0	%	60 mM phosphate buffer	6.25	25°C	10 mM p-Nitrophenyl N-acetyl-β-D-glucosaminide, 20 mM Sialylglycopeptide	p-Nitrophenyl-GlcNAc-oligosaccharide	—	—	Classical aqueous control (in %)
Activity + Stability - Product Formation	Product yield after incubation (3 hours at 25°C) in the presence of organic solvent, measured by HPLC	Dimethyl Sulfoxide (DMSO)	30%	14.7	21.3	%	60 mM phosphate buffer	6.25	25°C	10 mM p-Nitrophenyl N-acetyl-β-D-glucosaminide, 20 mM Sialylglycopeptide	p-Nitrophenyl-GlcNAc-oligosaccharide	—	—	Classical aqueous control (in %)
Activity + Stability - Product Formation	Product yield after incubation (3 hours at 25°C) in the presence of organic solvent, measured by HPLC	Methanol	30%	14.7	24.7	%	60 mM phosphate buffer	6.25	25°C	10 mM p-Nitrophenyl N-acetyl-β-D-glucosaminide, 20 mM Sialylglycopeptide	p-Nitrophenyl-GlcNAc-oligosaccharide	—	—	Classical aqueous control (in %)
Activity + Stability - Product Formation	Product yield after incubation (3 hours at 25°C) in the presence of organic solvent, measured by HPLC	Tetrahydrofuran (THF)	30%	14.7	0.0	%	60 mM phosphate buffer	6.25	25°C	10 mM p-Nitrophenyl N-acetyl-β-D-glucosaminide, 20 mM Sialylglycopeptide	p-Nitrophenyl-GlcNAc-oligosaccharide	—	—	Classical aqueous control (in %)
Activity + Stability - Product Formation	Product yield after incubation (3 hours at 25°C) in the presence of organic solvent, measured by HPLC	Acetone	10%	14.7	19.8	%	60 mM phosphate buffer	6.25	25°C	10 mM p-Nitrophenyl N-acetyl-β-D-glucosaminide, 20 mM Sialylglycopeptide	p-Nitrophenyl-GlcNAc-oligosaccharide	—	—	Classical aqueous control (in %)
Activity + Stability - Product Formation	Product yield after incubation (3 hours at 25°C) in the presence of organic solvent, measured by HPLC	Acetone	20%	14.7	26.4	%	60 mM phosphate buffer	6.25	25°C	10 mM p-Nitrophenyl N-acetyl-β-D-glucosaminide, 20 mM Sialylglycopeptide	p-Nitrophenyl-GlcNAc-oligosaccharide	—	—	Classical aqueous control (in %)
Activity + Stability - Product Formation	Product yield after incubation (3 hours at 25°C) in the presence of organic solvent, measured by HPLC	Acetone	40%	14.7	29.8	%	60 mM phosphate buffer	6.25	25°C	10 mM p-Nitrophenyl N-acetyl-β-D-glucosaminide, 20 mM Sialylglycopeptide	p-Nitrophenyl-GlcNAc-oligosaccharide	—	—	Classical aqueous control (in %)
Activity + Stability - Product Formation	Product yield after incubation (3 hours at 25°C) in the presence of organic solvent, measured by HPLC	Acetone	50%	14.7	4.7	%	60 mM phosphate buffer	6.25	25°C	10 mM p-Nitrophenyl N-acetyl-β-D-glucosaminide, 20 mM Sialylglycopeptide	p-Nitrophenyl-GlcNAc-oligosaccharide	—	—	Classical aqueous control (in %)

Activity + Stability - Product Formation	Product yield after incubation (3 hours at 25°C) in the presence of organic solvent, measured by HPLC	Acetone	30%	14.7	34.1	%	60 mM phosphate buffer	6.25	25°C	10 mM p-Nitrophenyl N-acetyl-β-D-glucosaminide, 20 mM Sialylglycopeptide	p-Nitrophenyl-GlcNAc-oligosaccharide	—	—	Classical aqueous control (in %)
Activity + Stability - Product Formation	Product yield after incubation (3 hours at 25°C) in the presence of organic solvent, measured by HPLC	2-Butanone	30%	14.7	0.0	%	60 mM phosphate buffer	6.25	25°C	10 mM p-Nitrophenyl N-acetyl-β-D-glucosaminide, 20 mM Sialylglycopeptide	p-Nitrophenyl-GlcNAc-oligosaccharide	—	—	Classical aqueous control (in %)
Activity + Stability - Product Formation	Product yield after incubation (3 hours at 25°C) in the presence of organic solvent, measured by HPLC	Acetonitrile	30%	14.7	0.0	%	60 mM phosphate buffer	6.25	25°C	10 mM p-Nitrophenyl N-acetyl-β-D-glucosaminide, 20 mM Sialylglycopeptide	p-Nitrophenyl-GlcNAc-oligosaccharide	—	—	Classical aqueous control (in %)
Activity + Stability - Product Formation	Product yield after incubation (3 hours at 25°C) in the presence of organic solvent, measured by HPLC	1,4-Dioxane	30%	14.7	0.0	%	60 mM phosphate buffer	6.25	25°C	10 mM p-Nitrophenyl N-acetyl-β-D-glucosaminide, 20 mM Sialylglycopeptide	p-Nitrophenyl-GlcNAc-oligosaccharide	—	—	Classical aqueous control (in %)
Activity + Stability - Product Formation	Product yield after incubation (3 hours at 25°C) in the presence of organic solvent, measured by HPLC	Dimethylformamide (DMF)	30%	14.7	0.0	%	60 mM phosphate buffer	6.25	25°C	10 mM p-Nitrophenyl N-acetyl-β-D-glucosaminide, 20 mM Sialylglycopeptide	p-Nitrophenyl-GlcNAc-oligosaccharide	—	—	Classical aqueous control (in %)
Activity + Stability - Product Formation	Product yield after incubation (3 hours at 25°C) in the presence of organic solvent, measured by HPLC	Dimethyl Sulfoxide (DMSO)	30%	14.7	21.3	%	60 mM phosphate buffer	6.25	25°C	10 mM p-Nitrophenyl N-acetyl-β-D-glucosaminide, 20 mM Sialylglycopeptide	p-Nitrophenyl-GlcNAc-oligosaccharide	—	—	Classical aqueous control (in %)
Activity + Stability - Product Formation	Product yield after incubation (3 hours at 25°C) in the presence of organic solvent, measured by HPLC	Methanol	30%	14.7	24.7	%	60 mM phosphate buffer	6.25	25°C	10 mM p-Nitrophenyl N-acetyl-β-D-glucosaminide, 20 mM Sialylglycopeptide	p-Nitrophenyl-GlcNAc-oligosaccharide	—	—	Classical aqueous control (in %)
Activity + Stability - Product Formation	Product yield after incubation (3 hours at 25°C) in the presence of organic solvent, measured by HPLC	Tetrahydrofuran (THF)	30%	14.7	0.0	%	60 mM phosphate buffer	6.25	25°C	10 mM p-Nitrophenyl N-acetyl-β-D-glucosaminide, 20 mM Sialylglycopeptide	p-Nitrophenyl-GlcNAc-oligosaccharide	—	—	Classical aqueous control (in %)
Activity + Stability - Product Formation	Product yield after incubation (3 hours at 25°C) in the presence of organic solvent, measured by HPLC	Acetone	10%	14.7	19.8	%	60 mM phosphate buffer	6.25	25°C	10 mM p-Nitrophenyl N-acetyl-β-D-glucosaminide, 20 mM Sialylglycopeptide	p-Nitrophenyl-GlcNAc-oligosaccharide	—	—	Classical aqueous control (in %)
Activity + Stability - Product Formation	Product yield after incubation (3 hours at 25°C) in the presence of organic solvent, measured by HPLC	Acetone	20%	14.7	26.4	%	60 mM phosphate buffer	6.25	25°C	10 mM p-Nitrophenyl N-acetyl-β-D-glucosaminide, 20 mM Sialylglycopeptide	p-Nitrophenyl-GlcNAc-oligosaccharide	—	—	Classical aqueous control (in %)
Activity + Stability - Product Formation	Product yield after incubation (3 hours at 25°C) in the presence of organic solvent, measured by HPLC	Acetone	40%	14.7	29.8	%	60 mM phosphate buffer	6.25	25°C	10 mM p-Nitrophenyl N-acetyl-β-D-glucosaminide, 20 mM Sialylglycopeptide	p-Nitrophenyl-GlcNAc-oligosaccharide	—	—	Classical aqueous control (in %)
Activity + Stability - Product Formation	Product yield after incubation (3 hours at 25°C) in the presence of organic solvent, measured by HPLC	Acetone	50%	14.7	4.7	%	60 mM phosphate buffer	6.25	25°C	10 mM p-Nitrophenyl N-acetyl-β-D-glucosaminide, 20 mM Sialylglycopeptide	p-Nitrophenyl-GlcNAc-oligosaccharide	—	—	Classical aqueous control (in %)

Visualization : Activity + Stability — Product Formation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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