Lipase (gene lip3) from Pseudomonas aeruginosa LST-03

Enzyme Description

Species
Pseudomonas aeruginosa LST-03 β†—
Extremophile
No
EC Number
3.1.1.3 β†—

Sequence

Length: 315 amino acids
MKRFLLGLVLLLAVAAGVLYFVPATLLASVRTVERGLAGLSEHSVQVDNLEIAYLEGGSEKNPTLLLIHGFGADKDNWLRFARPLTERYHVVALDLPGFGDSSKPQQASYDVGTQAERVANFAAAIGVRRLHLAGNSMGGHIAALYAARHPEQVLSLALIDNAGVMPARKSELFEDLERGENPLVVRQPEDFQKLLDFVFVQQPPLPAPLKRYLGERAVAASAFNAQIFEQLRQRYIPLEPELPKIEAPTLLLWGDRDRVLDVSSIEVMRPLLKRPSVVIMENCGHVPMVERPEETAQHYQAFLDGVRNAQVAGR
H. Ogino et al. (2004) β€” Cloning, expression and characterization of a lipase gene (lip3) from Pseudomonas aeruginosa LST-03
Molecular Genetics and Genomics  Β· doi:10.1007/s00438-003-0970-8 β†—  Β· Stability - Half-life
15 measurements ▼
Database ID
Q9KJG6_PSEAI
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli JM109

Experimental Data (15 measurements)

15 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Half-life Half-life in organic solvent, measured by residual activity with absorbance spectrophotometry (colorimetric assay, 2.3-dimercaptopropan-1-ol and DTNB reaction product absorbance measurement, 412 nm) Ethylene Glycol 25% 12.3 84.6 hours 100 mM Tris-HCl 8 Incubation: 30Β°C, Assay: 30Β°C 2 mM BALB 2,3-Dimercapto-1-propanol tributyrate 2,3-dimercaptopropan-1-ol,3butyric acid β€” β€” Classical aqueous control (in hours)
Stability - Half-life Half-life in organic solvent, measured by residual activity with absorbance spectrophotometry (colorimetric assay, 2.3-dimercaptopropan-1-ol and DTNB reaction product absorbance measurement, 412 nm) Glycerol 25% 12.3 37.0 hours 100 mM Tris-HCl 8 Incubation: 30Β°C, Assay: 30Β°C 2 mM BALB 2,3-Dimercapto-1-propanol tributyrate 2,3-dimercaptopropan-1-ol,3butyric acid β€” β€” Classical aqueous control (in hours)
Stability - Half-life Half-life in organic solvent, measured by residual activity with absorbance spectrophotometry (colorimetric assay, 2.3-dimercaptopropan-1-ol and DTNB reaction product absorbance measurement, 412 nm) n-Octane 25% 12.3 9.2 hours 100 mM Tris-HCl 8 Incubation: 30Β°C, Assay: 30Β°C 2 mM BALB 2,3-Dimercapto-1-propanol tributyrate 2,3-dimercaptopropan-1-ol,3butyric acid β€” β€” Classical aqueous control (in hours)
Stability - Half-life Half-life in organic solvent, measured by residual activity with absorbance spectrophotometry (colorimetric assay, 2.3-dimercaptopropan-1-ol and DTNB reaction product absorbance measurement, 412 nm) n-Heptane 25% 12.3 7.9 hours 100 mM Tris-HCl 8 Incubation: 30Β°C, Assay: 30Β°C 2 mM BALB 2,3-Dimercapto-1-propanol tributyrate 2,3-dimercaptopropan-1-ol,3butyric acid β€” β€” Classical aqueous control (in hours)
Stability - Half-life Half-life in organic solvent, measured by residual activity with absorbance spectrophotometry (colorimetric assay, 2.3-dimercaptopropan-1-ol and DTNB reaction product absorbance measurement, 412 nm) Dimethylformamide (DMF) 25% 12.3 7.9 hours 100 mM Tris-HCl 8 Incubation: 30Β°C, Assay: 30Β°C 2 mM BALB 2,3-Dimercapto-1-propanol tributyrate 2,3-dimercaptopropan-1-ol,3butyric acid β€” β€” Classical aqueous control (in hours)
Stability - Half-life Half-life in organic solvent, measured by residual activity with absorbance spectrophotometry (colorimetric assay, 2.3-dimercaptopropan-1-ol and DTNB reaction product absorbance measurement, 412 nm) n-Hexane 25% 12.3 7.7 hours 100 mM Tris-HCl 8 Incubation: 30Β°C, Assay: 30Β°C 2 mM BALB 2,3-Dimercapto-1-propanol tributyrate 2,3-dimercaptopropan-1-ol,3butyric acid β€” β€” Classical aqueous control (in hours)
Stability - Half-life Half-life in organic solvent, measured by residual activity with absorbance spectrophotometry (colorimetric assay, 2.3-dimercaptopropan-1-ol and DTNB reaction product absorbance measurement, 412 nm) 1,4-Butanediol 25% 12.3 7.4 hours 100 mM Tris-HCl 8 Incubation: 30Β°C, Assay: 30Β°C 2 mM BALB 2,3-Dimercapto-1-propanol tributyrate 2,3-dimercaptopropan-1-ol,3butyric acid β€” β€” Classical aqueous control (in hours)
Stability - Half-life Half-life in organic solvent, measured by residual activity with absorbance spectrophotometry (colorimetric assay, 2.3-dimercaptopropan-1-ol and DTNB reaction product absorbance measurement, 412 nm) Dimethyl Sulfoxide (DMSO) 25% 12.3 3.4 hours 100 mM Tris-HCl 8 Incubation: 30Β°C, Assay: 30Β°C 2 mM BALB 2,3-Dimercapto-1-propanol tributyrate 2,3-dimercaptopropan-1-ol,3butyric acid β€” β€” Classical aqueous control (in hours)
Stability - Half-life Half-life in organic solvent, measured by residual activity with absorbance spectrophotometry (colorimetric assay, 2.3-dimercaptopropan-1-ol and DTNB reaction product absorbance measurement, 412 nm) Cyclohexane 25% 12.3 0.48 hours 100 mM Tris-HCl 8 Incubation: 30Β°C, Assay: 30Β°C 2 mM BALB 2,3-Dimercapto-1-propanol tributyrate 2,3-dimercaptopropan-1-ol,3butyric acid β€” β€” Classical aqueous control (in hours)
Stability - Half-life Half-life in organic solvent, measured by residual activity with absorbance spectrophotometry (colorimetric assay, 2.3-dimercaptopropan-1-ol and DTNB reaction product absorbance measurement, 412 nm) n-Decane 25% 12.3 0.4 hours 100 mM Tris-HCl 8 Incubation: 30Β°C, Assay: 30Β°C 2 mM BALB 2,3-Dimercapto-1-propanol tributyrate 2,3-dimercaptopropan-1-ol,3butyric acid β€” β€” Classical aqueous control (in hours)
Stability - Half-life Half-life in organic solvent, measured by residual activity with absorbance spectrophotometry (colorimetric assay, 2.3-dimercaptopropan-1-ol and DTNB reaction product absorbance measurement, 412 nm) Toluene 25% 12.3 0.37 hours 100 mM Tris-HCl 8 Incubation: 30Β°C, Assay: 30Β°C 2 mM BALB 2,3-Dimercapto-1-propanol tributyrate 2,3-dimercaptopropan-1-ol,3butyric acid β€” β€” Classical aqueous control (in hours)
Stability - Half-life Half-life in organic solvent, measured by residual activity with absorbance spectrophotometry (colorimetric assay, 2.3-dimercaptopropan-1-ol and DTNB reaction product absorbance measurement, 412 nm) Methanol 25% 12.3 0.29 hours 100 mM Tris-HCl 8 Incubation: 30Β°C, Assay: 30Β°C 2 mM BALB 2,3-Dimercapto-1-propanol tributyrate 2,3-dimercaptopropan-1-ol,3butyric acid β€” β€” Classical aqueous control (in hours)
Stability - Half-life Half-life in organic solvent, measured by residual activity with absorbance spectrophotometry (colorimetric assay, 2.3-dimercaptopropan-1-ol and DTNB reaction product absorbance measurement, 412 nm) Ethanol 25% 12.3 0.2 hours 100 mM Tris-HCl 8 Incubation: 30Β°C, Assay: 30Β°C 2 mM BALB 2,3-Dimercapto-1-propanol tributyrate 2,3-dimercaptopropan-1-ol,3butyric acid β€” β€” Classical aqueous control (in hours)
Stability - Half-life Half-life in organic solvent, measured by residual activity with absorbance spectrophotometry (colorimetric assay, 2.3-dimercaptopropan-1-ol and DTNB reaction product absorbance measurement, 412 nm) 1,5-Pentanediol 25% 12.3 0.19 hours 100 mM Tris-HCl 8 Incubation: 30Β°C, Assay: 30Β°C 2 mM BALB 2,3-Dimercapto-1-propanol tributyrate 2,3-dimercaptopropan-1-ol,3butyric acid β€” β€” Classical aqueous control (in hours)
Stability - Half-life Half-life in organic solvent, measured by residual activity with absorbance spectrophotometry (colorimetric assay, 2.3-dimercaptopropan-1-ol and DTNB reaction product absorbance measurement, 412 nm) tert-Butanol 25% 12.3 0.17 hours 100 mM Tris-HCl 8 Incubation: 30Β°C, Assay: 30Β°C 2 mM BALB 2,3-Dimercapto-1-propanol tributyrate 2,3-dimercaptopropan-1-ol,3butyric acid β€” β€” Classical aqueous control (in hours)

Visualization : Stability β€” Half-life

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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