Aldehyde dehydrogenase (gene ALDH) from Sulfolobus tokodaii strain 7

Enzyme Description

Species

Sulfolobus tokodaii strain 7 ↗

Extremophile

Yes "hyperthermophilic" organism

EC Number

1.2.1.5 ↗

Sequence

Length: 468 amino acids

MSEVIEIKSPSNLKVIGTVKRMSKDEVRGEIEEAYKGFETISRMPLYKRTAILRKVSEILEREQERLARLLAMEAGKPIKDSRVEVMRASRLFRQAAEEAAIVLEGKNYRVDAYEYPPGNENRIVISTREPIGVVTAILPFNFPINSFAHKVAPAIAVGNSVVVKPSISTPLSAIEMKKILVEAGLPDSAVRIVTGYSNEIGDELITHPLVGLITLTGSTQTGLAIASKAVSLGKRIIMELGGSDPIIVLEDANIDRASSIAVRARYEYAGQNCNAGKRIIVREEIYDKFVKAFKEKVKALKVGDPLDESTDIGPVINQESVEKLNKALEDAQSKGGNVEVLNKGPETGYFFPLSLVTNPSLDMLVLKTEIFGPIAPIVSVKSDEEAINIANSTEYGLQSAIFSNDVNRALKIAKELKFGAIIINDSTRLRWDSLPFGGFKKTGIGREGVRDTMLEMTENKLIAITLL

Tianming Liu et al. (2013) — Molecular characterization of a thermostable aldehyde dehydrogenase (ALDH) from the hyperthermophilic archaeon Sulfolobus tokodaii strain 7

Extremophiles · doi:10.1007/s00792-012-0503-7 ↗ · Activity - Classical

6 measurements ▼

Database ID

UniProt: Q976X5 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (6 measurements)

6 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	10%	100	93	%	50 mM Tris-HCl buffer	8	80°C	1 mM Acetaldehyde	Acetate	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	10%	100	98	%	50 mM Tris-HCl buffer	8	80°C	1 mM Acetaldehyde	Acetate	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Ethylene Glycol	10%	100	40	%	50 mM Tris-HCl buffer	8	80°C	1 mM Acetaldehyde	Acetate	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Isopropanol	10%	100	92	%	50 mM Tris-HCl buffer	8	80°C	1 mM Acetaldehyde	Acetate	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetone	10%	100	79	%	50 mM Tris-HCl buffer	8	80°C	1 mM Acetaldehyde	Acetate	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	68	%	50 mM Tris-HCl buffer	8	80°C	1 mM Acetaldehyde	Acetate	1 mM NADP+	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	10%	100	93	%	50 mM Tris-HCl buffer	8	80°C	1 mM Acetaldehyde	Acetate	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	10%	100	98	%	50 mM Tris-HCl buffer	8	80°C	1 mM Acetaldehyde	Acetate	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Ethylene Glycol	10%	100	40	%	50 mM Tris-HCl buffer	8	80°C	1 mM Acetaldehyde	Acetate	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Isopropanol	10%	100	92	%	50 mM Tris-HCl buffer	8	80°C	1 mM Acetaldehyde	Acetate	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetone	10%	100	79	%	50 mM Tris-HCl buffer	8	80°C	1 mM Acetaldehyde	Acetate	1 mM NADP+	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	68	%	50 mM Tris-HCl buffer	8	80°C	1 mM Acetaldehyde	Acetate	1 mM NADP+	—	Classical aqueous control (in %)

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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