Amidase PsAmiA from Pseudomonas stutzeri CGMCC 22915
Enzyme Description
Sequence
MSSGANLPSLREIRELSEKFGLELSDDDVKSYQTLMRGPILSTRRIEELVEFRPPVKYPRTPGYRPSAEQNPYNAWYWRCNIEGAPSGILKGYEVGVKDPIGVAGLPLMNGSRMLEGYISEVDATVVTRVLDAGATIVGKTTTEETSLSAGGHTCAYGPIRNPRKPTHSPGGSSGGSAAAVAAGDVKMCLGADQAGSIRLPASRCGIVGLKATHGLVPYTGAMMIEMTMDHIGPMADTVENTARLLTAIAGPDPLDPRQRGVIPADYVRDYMPSIGRGVKGLRIGVLKEGFGQKPWADLGFPGSDEIVDQKCRAAVKALEKQGAIVSEVSIPMHLDGPHLWNGICIEGAASFMILGNNHGTNWRGFYNTPLLDAVGRGFNTRAKDLSVQAKLVLMMGEYLRGEYHGRYYAKAQNTRHLLNAAYDRALQEVDVLVMPTIPFVTPELAKPDCSIEEDMMAALSCIANTCPFDVSGHPAISVPCGTVDGLPVGLMIVGRHFEDIVVLQAADAVEQTGDWRSQ
Huoyong Jiang et al. (2023)
β Novel biodegradation pathway of insecticide flonicamid mediated by an amidase and its unusual substrate spectrum
Journal of Hazardous Materials
Β· doi:10.1016/j.jhazmat.2022.129952 β
Β· Activity - Classical
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Database ID
UniParc: UPI00206A42D3 β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli Rosetta
Experimental Data (8 measurements)
8 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by HPLC in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 1% | 100.0 | 88.6 | % | PBS buffer | 7 | 30Β°C | 200 mg/L 4-trifluoromethylnicotinamide | 5-trifluoromethylnicotinic acid , NH3β | β | 800 rpm | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by HPLC in the presence of organic solvent | Dichloromethane | 1% | 100.0 | 34.8 | % | PBS buffer | 7 | 30Β°C | 200 mg/L 4-trifluoromethylnicotinamide | 5-trifluoromethylnicotinic acid , NH3β | β | 800 rpm | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by HPLC in the presence of organic solvent | Cyclohexane | 1% | 100.0 | 99.3 | % | PBS buffer | 7 | 30Β°C | 200 mg/L 4-trifluoromethylnicotinamide | 5-trifluoromethylnicotinic acid , NH3β | β | 800 rpm | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by HPLC in the presence of organic solvent | Methanol | 1% | 100.0 | 86.2 | % | PBS buffer | 7 | 30Β°C | 200 mg/L 4-trifluoromethylnicotinamide | 5-trifluoromethylnicotinic acid , NH3β | β | 800 rpm | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by HPLC in the presence of organic solvent | Ethanol | 1% | 100.0 | 79.7 | % | PBS buffer | 7 | 30Β°C | 200 mg/L 4-trifluoromethylnicotinamide | 5-trifluoromethylnicotinic acid , NH3β | β | 800 rpm | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by HPLC in the presence of organic solvent | Isopropanol | 1% | 100.0 | 78.0 | % | PBS buffer | 7 | 30Β°C | 200 mg/L 4-trifluoromethylnicotinamide | 5-trifluoromethylnicotinic acid , NH3β | β | 800 rpm | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by HPLC in the presence of organic solvent | 1-Butanol | 1% | 100.0 | 30.0 | % | PBS buffer | 7 | 30Β°C | 200 mg/L 4-trifluoromethylnicotinamide | 5-trifluoromethylnicotinic acid , NH3β | β | 800 rpm | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by HPLC in the presence of organic solvent | Isoamyl Alcohol | 1% | 100.0 | 15.4 | % | PBS buffer | 7 | 30Β°C | 200 mg/L 4-trifluoromethylnicotinamide | 5-trifluoromethylnicotinic acid , NH3β | β | 800 rpm | Classical aqueous control (in %) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.