Lipase from Pseudomonas fluorescens HK44

Enzyme Description

Species

Extremophile

EC Number

3.1.1.3 ↗

Sequence

Length: 290 amino acids

MNMDASTQYPIVLVHGLFGFDTIAGYPYFFDIKEALEYAGARVFAVNIPAVNGNEERGEKLLEQVNRILQETGAARVNLIGHSQGPLAARYVAALYPEKVASVTSVSCPNHGSEIADQLHKALTPGELPEAFALALLSAVGTFISLISGHPKNPQDPQAAFESLTSAGLAEFNRKYPQGVPKVWGGEGNEIENGVYYYSWSGILQNFQTPQILDPTHINCIVLSKLFYKEKDANDGLVGRYSSHLGKVIRSDYPMDHFDAVNQMAGITSWNVSPVSLYVEHAVRLKSKGL

Qiong Wang et al. (2025) — Data-Driven Mining of a Thermostable Lipase with Molecular Insights into Mechanisms of Its Substrate Specificity

Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.5c02801 ↗ · Activity + Stability - Incubation

5 measurements ▼

Database ID

UniProt: A0A010RUQ6 ↗

Sequence Annotation

Explicit - Provided UniProt Accesion Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (5 measurements)

5 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity + Stability - Incubation	Activity after incubation (1 hour at ?°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	Methanol	10%	100.0	71.7	%	Incubation: 50 mM Tris-HCl buffer, Assay: ? mM Tris-HCl buffer	Incubation: 7, Assay: 7	Incubation: ?, Assay: 37°C	? mM p-Nitrophenyl palmitate	p-Nitrophenol , Palmitic Acid	—	—	Non-incubated control (in %), assay with organic solvent \| Uncertainty about control and whether assay was with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (1 hour at ?°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	Ethanol	10%	100.0	64.7	%	Incubation: 50 mM Tris-HCl buffer, Assay: ? mM Tris-HCl buffer	Incubation: 7, Assay: 7	Incubation: ?, Assay: 37°C	? mM p-Nitrophenyl palmitate	p-Nitrophenol , Palmitic Acid	—	—	Non-incubated control (in %), assay with organic solvent \| Uncertainty about control and whether assay was with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (1 hour at ?°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	Isopropanol	10%	100.0	62.9	%	Incubation: 50 mM Tris-HCl buffer, Assay: ? mM Tris-HCl buffer	Incubation: 7, Assay: 7	Incubation: ?, Assay: 37°C	? mM p-Nitrophenyl palmitate	p-Nitrophenol , Palmitic Acid	—	—	Non-incubated control (in %), assay with organic solvent \| Uncertainty about control and whether assay was with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (1 hour at ?°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1-Butanol	10%	100.0	75.2	%	Incubation: 50 mM Tris-HCl buffer, Assay: ? mM Tris-HCl buffer	Incubation: 7, Assay: 7	Incubation: ?, Assay: 37°C	? mM p-Nitrophenyl palmitate	p-Nitrophenol , Palmitic Acid	—	—	Non-incubated control (in %), assay with organic solvent \| Uncertainty about control and whether assay was with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (1 hour at ?°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	Isoamyl Alcohol	10%	100.0	87.2	%	Incubation: 50 mM Tris-HCl buffer, Assay: ? mM Tris-HCl buffer	Incubation: 7, Assay: 7	Incubation: ?, Assay: 37°C	? mM p-Nitrophenyl palmitate	p-Nitrophenol , Palmitic Acid	—	—	Non-incubated control (in %), assay with organic solvent \| Uncertainty about control and whether assay was with or without organic solvent

Activity + Stability - Incubation	Activity after incubation (1 hour at ?°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	Methanol	10%	100.0	71.7	%	Incubation: 50 mM Tris-HCl buffer, Assay: ? mM Tris-HCl buffer	Incubation: 7, Assay: 7	Incubation: ?, Assay: 37°C	? mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay with organic solvent \| Uncertainty about control and whether assay was with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (1 hour at ?°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	Ethanol	10%	100.0	64.7	%	Incubation: 50 mM Tris-HCl buffer, Assay: ? mM Tris-HCl buffer	Incubation: 7, Assay: 7	Incubation: ?, Assay: 37°C	? mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay with organic solvent \| Uncertainty about control and whether assay was with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (1 hour at ?°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	Isopropanol	10%	100.0	62.9	%	Incubation: 50 mM Tris-HCl buffer, Assay: ? mM Tris-HCl buffer	Incubation: 7, Assay: 7	Incubation: ?, Assay: 37°C	? mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay with organic solvent \| Uncertainty about control and whether assay was with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (1 hour at ?°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1-Butanol	10%	100.0	75.2	%	Incubation: 50 mM Tris-HCl buffer, Assay: ? mM Tris-HCl buffer	Incubation: 7, Assay: 7	Incubation: ?, Assay: 37°C	? mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay with organic solvent \| Uncertainty about control and whether assay was with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (1 hour at ?°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	Isoamyl Alcohol	10%	100.0	87.2	%	Incubation: 50 mM Tris-HCl buffer, Assay: ? mM Tris-HCl buffer	Incubation: 7, Assay: 7	Incubation: ?, Assay: 37°C	? mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay with organic solvent \| Uncertainty about control and whether assay was with or without organic solvent

Visualization : Activity + Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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