Lipase from Bacillus sphaericus 205y
Enzyme Description
Sequence
MNQITNWRPRGFLQWLLTILSVIVCFIIIIVAYYIFNPSNMDKIMSLLAWGTSIFPIMLVVTAFIIIVLLALSFWKKTFIALTVLFPILLLLMFLTVQPISTMKSYAKSENVSVALSSHFFYNQNISTKPSVDVVYGKTTDGIELKLDVWPAKKKSEDVLTPVIVQVHGGGWVSGDKGQVQDWNQWMNDQGYTVFDVQYRMPPVAGWKDEVGDVKSAIGWIVQHADTYKIDPNRIILMGESAGGNLAMLAAYSLGDKHLPPSTDVPDVPIKAVINMYGPSDMTAFYKNNPSKRYVQDVLDQYIGGSPSDYPARYKKLSPISYIQEHTPPTIMFLGTGDRIVPVEQANVLDDKLTTSGVAHELYLLPKVDHGFDANPGSLSTQFAKEKVKAFLQKYNK
R. N. Z. A. Rahman et al. (2003)
β Cloning and expression of a novel lipase gene from Bacillus sphaericus 205y
Molecular Genetics and Genomics
Β· doi:10.1007/s00438-003-0831-5 β
Β· Stability - Incubation
▼
Database ID
UniProt: Q8VQP2 β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21
Experimental Data (5 measurements)
5 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (30 minutes at 37Β°C) measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 25% | 100 | 10 | % | Incubation: Crude enzyme, Assay: Crude enzyme (1ml), Olive oil emulsion (2.5ml), 0.01 M calcium chloride | ? | Incubation: 37Β°C, Assay: 37Β°C | ? mM Olive oil | free fatty acids , glycerol | β | Incubation: 200 rpm, Assay: 200 rpm | Water-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 37Β°C) measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in aqueous phase | Acetonitrile | 25% | 100 | 0 | % | Incubation: Crude enzyme, Assay: Crude enzyme (1ml), Olive oil emulsion (2.5ml), 0.01 M calcium chloride | ? | Incubation: 37Β°C, Assay: 37Β°C | ? mM Olive oil | free fatty acids , glycerol | β | Incubation: 200 rpm, Assay: 200 rpm | Water-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 37Β°C) measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in aqueous phase | p-Xylene | 25% | 100 | 90 | % | Incubation: Crude enzyme, Assay: Crude enzyme (1ml), Olive oil emulsion (2.5ml), 0.01 M calcium chloride | ? | Incubation: 37Β°C, Assay: 37Β°C | ? mM Olive oil | free fatty acids , glycerol | β | Incubation: 200 rpm, Assay: 200 rpm | Water-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 37Β°C) measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in aqueous phase | n-Hexane | 25% | 100 | 110 | % | Incubation: Crude enzyme, Assay: Crude enzyme (1ml), Olive oil emulsion (2.5ml), 0.01 M calcium chloride | ? | Incubation: 37Β°C, Assay: 37Β°C | ? mM Olive oil | free fatty acids , glycerol | β | Incubation: 200 rpm, Assay: 200 rpm | Water-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 37Β°C) measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in aqueous phase | n-Hexadecane | 25% | 100 | 0 | % | Incubation: Crude enzyme, Assay: Crude enzyme (1ml), Olive oil emulsion (2.5ml), 0.01 M calcium chloride | ? | Incubation: 37Β°C, Assay: 37Β°C | ? mM Olive oil | free fatty acids , glycerol | β | Incubation: 200 rpm, Assay: 200 rpm | Water-incubated control (in %), assay in aqueous phase |
Visualization : Stability β Incubation
R. N. Z. A. Rahman et al. (2003)
One bar per measurement. Colour = solvent, shade = solvent volume.
Moohamad Ropaning Sulong et al. (2006)
β A novel organic solvent tolerant lipase from Bacillus sphaericus 205y: Extracellular expression of a novel OST-lipase gene
Protein Expression and Purification
Β· doi:10.1016/j.pep.2006.04.015 β
Β· Stability - Incubation
▼
Database ID
UniProt: Q8VQP2 β
Sequence Annotation
Inferred - from protein name
Protein Source
Recombinant, host bacterium Escherichia coli TOP10
Experimental Data (11 measurements)
11 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (30 minutes at 37Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product) in aqueous phase | Dimethyl Sulfoxide (DMSO) | ?% | 100 | 176 | % | ? | ? | Incubation: 30Β°C, Assay: 37Β°C | ? mM Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 37Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product) in aqueous phase | Methanol | ?% | 100 | 120 | % | ? | ? | Incubation: 30Β°C, Assay: 37Β°C | ? mM Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 37Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product) in aqueous phase | Ethanol | ?% | 100 | 96 | % | ? | ? | Incubation: 30Β°C, Assay: 37Β°C | ? mM Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 37Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product) in aqueous phase | Acetonitrile | ?% | 100 | 26 | % | ? | ? | Incubation: 30Β°C, Assay: 37Β°C | ? mM Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 37Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product) in aqueous phase | 1-Butanol | ?% | 100 | 23 | % | ? | ? | Incubation: 30Β°C, Assay: 37Β°C | ? mM Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 37Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product) in aqueous phase | Toluene | ?% | 100 | 40 | % | ? | ? | Incubation: 30Β°C, Assay: 37Β°C | ? mM Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 37Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product) in aqueous phase | p-Xylene | ?% | 100 | 120 | % | ? | ? | Incubation: 30Β°C, Assay: 37Β°C | ? mM Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 37Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product) in aqueous phase | 1-Decanol | ?% | 100 | 55 | % | ? | ? | Incubation: 30Β°C, Assay: 37Β°C | ? mM Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 37Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product) in aqueous phase | n-Decane | ?% | 100 | 110 | % | ? | ? | Incubation: 30Β°C, Assay: 37Β°C | ? mM Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 37Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product) in aqueous phase | n-Dodecane | ?% | 100 | 43 | % | ? | ? | Incubation: 30Β°C, Assay: 37Β°C | ? mM Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 37Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product) in aqueous phase | n-Hexadecane | ?% | 100 | 60 | % | ? | ? | Incubation: 30Β°C, Assay: 37Β°C | ? mM Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether the assay really was in aqueous phase |
Visualization : Stability β Incubation
Moohamad Ropaning Sulong et al. (2006)
One bar per measurement. Colour = solvent, shade = solvent volume.