Superoxide dismutase from Cohnella sp. A01

Enzyme Description

Species
Cohnella sp. A01 β†—
Extremophile
Yes "thermophilic" organism
EC Number
1.15.1.1 β†—

Sequence

Length: 204 amino acids
MAHVLPPLPYAKDALEPYIDATTMEIHHDRHHNTYVTNLNKALESAPELANKSVEELISDLNAVPESIRTAVRNNGGGHANHSLFWETIGPNGGGAPTGALAAAIDSELGGFEKFKETFSNAAATRFGSGWAWLALGKDGKLKVYSLPNQDSPLMEGDTPLLGLDVWEHAYYLKYQNKRPDYIAAFWNVVNWEKVGALYDAAKK
Zahra Karimi Mazraeh Shahi et al. (2021) β€” Thermophilic iron containing type superoxide dismutase from Cohnella sp. A01
International Journal of Biological Macromolecules  Β· doi:10.1016/j.ijbiomac.2021.07.150 β†—  Β· Activity - Classical
15 measurements ▼
Database ID
NCBI: KX857493.1 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (15 measurements)

15 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (measurement of NBT-formazan absorbance decline due to superoxide radicals production, 560 nm) in the presence of organic solvent Methanol 5% 100.0 91.9 % 100 mM potassium phosphate buffer, 0.5 MM EDTA, 7 Β΅M riboflavin, 25 mM methionine, 90 Β΅M NBT 8.2 45Β°C ? mM H+, ? mM O₂⁻ Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) , Oβ‚‚ β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurement of NBT-formazan absorbance decline due to superoxide radicals production, 560 nm) in the presence of organic solvent Methanol 10% 100.0 106.7 % 100 mM potassium phosphate buffer, 0.5 MM EDTA, 7 Β΅M riboflavin, 25 mM methionine, 90 Β΅M NBT 8.2 45Β°C ? mM H+, ? mM O₂⁻ Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) , Oβ‚‚ β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurement of NBT-formazan absorbance decline due to superoxide radicals production, 560 nm) in the presence of organic solvent Ethanol 5% 100.0 107.4 % 100 mM potassium phosphate buffer, 0.5 MM EDTA, 7 Β΅M riboflavin, 25 mM methionine, 90 Β΅M NBT 8.2 45Β°C ? mM H+, ? mM O₂⁻ Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) , Oβ‚‚ β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurement of NBT-formazan absorbance decline due to superoxide radicals production, 560 nm) in the presence of organic solvent Ethanol 10% 100.0 108.0 % 100 mM potassium phosphate buffer, 0.5 MM EDTA, 7 Β΅M riboflavin, 25 mM methionine, 90 Β΅M NBT 8.2 45Β°C ? mM H+, ? mM O₂⁻ Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) , Oβ‚‚ β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurement of NBT-formazan absorbance decline due to superoxide radicals production, 560 nm) in the presence of organic solvent Isopropanol 5% 100.0 106.0 % 100 mM potassium phosphate buffer, 0.5 MM EDTA, 7 Β΅M riboflavin, 25 mM methionine, 90 Β΅M NBT 8.2 45Β°C ? mM H+, ? mM O₂⁻ Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) , Oβ‚‚ β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurement of NBT-formazan absorbance decline due to superoxide radicals production, 560 nm) in the presence of organic solvent Isopropanol 10% 100.0 104.7 % 100 mM potassium phosphate buffer, 0.5 MM EDTA, 7 Β΅M riboflavin, 25 mM methionine, 90 Β΅M NBT 8.2 45Β°C ? mM H+, ? mM O₂⁻ Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) , Oβ‚‚ β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurement of NBT-formazan absorbance decline due to superoxide radicals production, 560 nm) in the presence of organic solvent Isobutanol 5% 100.0 118.1 % 100 mM potassium phosphate buffer, 0.5 MM EDTA, 7 Β΅M riboflavin, 25 mM methionine, 90 Β΅M NBT 8.2 45Β°C ? mM H+, ? mM O₂⁻ Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) , Oβ‚‚ β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurement of NBT-formazan absorbance decline due to superoxide radicals production, 560 nm) in the presence of organic solvent Glycerol 5% 100.0 110.1 % 100 mM potassium phosphate buffer, 0.5 MM EDTA, 7 Β΅M riboflavin, 25 mM methionine, 90 Β΅M NBT 8.2 45Β°C ? mM H+, ? mM O₂⁻ Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) , Oβ‚‚ β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurement of NBT-formazan absorbance decline due to superoxide radicals production, 560 nm) in the presence of organic solvent Glycerol 10% 100.0 130.2 % 100 mM potassium phosphate buffer, 0.5 MM EDTA, 7 Β΅M riboflavin, 25 mM methionine, 90 Β΅M NBT 8.2 45Β°C ? mM H+, ? mM O₂⁻ Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) , Oβ‚‚ β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurement of NBT-formazan absorbance decline due to superoxide radicals production, 560 nm) in the presence of organic solvent Acetone 5% 100.0 94.0 % 100 mM potassium phosphate buffer, 0.5 MM EDTA, 7 Β΅M riboflavin, 25 mM methionine, 90 Β΅M NBT 8.2 45Β°C ? mM H+, ? mM O₂⁻ Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) , Oβ‚‚ β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurement of NBT-formazan absorbance decline due to superoxide radicals production, 560 nm) in the presence of organic solvent Acetone 10% 100.0 108.7 % 100 mM potassium phosphate buffer, 0.5 MM EDTA, 7 Β΅M riboflavin, 25 mM methionine, 90 Β΅M NBT 8.2 45Β°C ? mM H+, ? mM O₂⁻ Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) , Oβ‚‚ β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurement of NBT-formazan absorbance decline due to superoxide radicals production, 560 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 5% 100.0 112.7 % 100 mM potassium phosphate buffer, 0.5 MM EDTA, 7 Β΅M riboflavin, 25 mM methionine, 90 Β΅M NBT 8.2 45Β°C ? mM H+, ? mM O₂⁻ Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) , Oβ‚‚ β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurement of NBT-formazan absorbance decline due to superoxide radicals production, 560 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10% 100.0 118.8 % 100 mM potassium phosphate buffer, 0.5 MM EDTA, 7 Β΅M riboflavin, 25 mM methionine, 90 Β΅M NBT 8.2 45Β°C ? mM H+, ? mM O₂⁻ Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) , Oβ‚‚ β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurement of NBT-formazan absorbance decline due to superoxide radicals production, 560 nm) in the presence of organic solvent n-Hexane 5% 100.0 41.6 % 100 mM potassium phosphate buffer, 0.5 MM EDTA, 7 Β΅M riboflavin, 25 mM methionine, 90 Β΅M NBT 8.2 45Β°C ? mM H+, ? mM O₂⁻ Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) , Oβ‚‚ β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (measurement of NBT-formazan absorbance decline due to superoxide radicals production, 560 nm) in the presence of organic solvent n-Hexane 10% 100.0 40.3 % 100 mM potassium phosphate buffer, 0.5 MM EDTA, 7 Β΅M riboflavin, 25 mM methionine, 90 Β΅M NBT 8.2 45Β°C ? mM H+, ? mM O₂⁻ Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) , Oβ‚‚ β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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