Ξ±-L-rhamnosidase (gene TsRha) from Thermotoga sp. 2812B
Enzyme Description
Sequence
MLRLENLRCEYMRNPINIDVEKPRFSWILISDQRDQFQRAYRIIVSSSYEKALNWIGDIWDSGKVLSGENINVEYGGKELESFTRYYWRVKCWTDNEEAESDIAFFETAALEEEDWKAKWITKKEFISFISNENPASGQDKCKQYYAAYFRKEFEITKNVKRARAYICGLGIYELRINGRKVGDNVLDPGQTDYSKIALYSTYDITDYLNEKNAIGVIVGNGRHIESYGYGKPRLIAQFLIEYEDGTREFLVTDENWRVSHGPLMENGIYYGERYDARLEMPGWDEYGFDDSSWEEVEATNRPKLKSQMMPPIKITETLKPKKMWSPKPGVYVFDFGQNFTGWVRLKVRGPRGTEIKIRYAELVDNDGTLNTSTLRSAESTDVYILKGAGEEIYEPRFTYHGFRYVEITGYPGVPTLESVEGRFVHTAVEKIGDFVCSNDLVNQIHKNIIWGQLSNLMSIPTDCPQRDERMGWLGDAQLTAEEAILNFDMAAFYTKYLMDIRLSQREDGSIPDVVPPYWKLYPADPAWGTAYITIAWYMYLYYNDKRVLEEHYDSMKKYVEFLKDNSDNYIIRNLGKYGDWCPPGSIHPKGTPLELTSTFYFYHDALLLSKIAKVLNKKIDVEKYKRLSENIKNAFNGEFLKFEKGKHIYSGCGCNKKSTNQTLCVLPIYSNIVPEEYKLEVFKSLEELVEVRSDGHLDTGIIGTRYLLEVLSDNGRFDLAYKIVTQETYPGWGYMIKEGATTLWERWEKLCNGGMNSHNHIMFGSIDAWFYKYVAGIRILEPGWKKFQVKPPINADLQFASARLIAIQGEIFVSWERLNGKFRMVVSIPANTQALVYVPLMSKKEVFESGRKVFPSENEGKICGISFVEMKDKFIVLKIGSGWYNFESF
Bin-Chun Li et al. (2025)
β Characterization of a Thermophilic and Acidophilic GH78 Ξ±-L-Rhamnosidase from Thermotoga sp. 2812B Capable of Efficiently Hydrolyzing a Variety of Natural Flavonoid Diglycosides
Biomolecules
Β· doi:10.3390/biom16010068 β
Β· Activity - Classical
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Database ID
NCBI: AIY87265.1 β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (6 measurements)
6 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by HPLC in the presence of organic solvent | Methanol | 10% | 100 | 54 | % | 45 mM citric-acid buffer, 5% DMSO to dissolve substrate | 5 | 90Β°C | 1 mM Naringin | L-Rhamnose , Prunin | β | 800 rpm | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by HPLC in the presence of organic solvent | Ethanol | 10% | 100 | 44 | % | 45 mM citric-acid buffer, 5% DMSO to dissolve substrate | 5 | 90Β°C | 1 mM Naringin | L-Rhamnose , Prunin | β | 800 rpm | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by HPLC in the presence of organic solvent | Ethylene Glycol | 10% | 100 | 55 | % | 45 mM citric-acid buffer, 5% DMSO to dissolve substrate | 5 | 90Β°C | 1 mM Naringin | L-Rhamnose , Prunin | β | 800 rpm | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by HPLC in the presence of organic solvent | Isopropanol | 10% | 100 | 60 | % | 45 mM citric-acid buffer, 5% DMSO to dissolve substrate | 5 | 90Β°C | 1 mM Naringin | L-Rhamnose , Prunin | β | 800 rpm | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by HPLC in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 10% | 100 | 47 | % | 45 mM citric-acid buffer, 5% DMSO to dissolve substrate | 5 | 90Β°C | 1 mM Naringin | L-Rhamnose , Prunin | β | 800 rpm | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by HPLC in the presence of organic solvent | Glycerol | 10% | 100 | 63 | % | 45 mM citric-acid buffer, 5% DMSO to dissolve substrate | 5 | 90Β°C | 1 mM Naringin | L-Rhamnose , Prunin | β | 800 rpm | Classical aqueous control (in %) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.