Lipase (gene PCLase0801) from Pseudomonas sp. DS0801

Enzyme Description

Species
Pseudomonas sp. DS0801 β†—
Extremophile
No
EC Number
3.1.1.3 β†—

Sequence

Length: 311 amino acids
MKKKSLLPLGLAIGLAPLAASPLIQASTYTQTKYPNVLAHGMLGFDNILGVDYWFGIPSALRRDGAQVYVTEVSQLDTSEVRGEQLLLQVEEIVALSGQPKVNLIGHSHGGPTIRYVAAVRPDLIASATSVGAPHKGSDTADFLRQIPPGSAGEAVLSGLVNSLGALISFLSSGSAGTQNSLGSLESLNSEGAARFNAKYPQGIPTSACGEGAYKVNGVSYYSWSGSSPLTNFLDPSDAFLGASSLTFKNGTANDGLVGTCSSHLGMVIRDNYRMNHLDEVNQVFGLTSLFETSPVSVYRQHANRLKNASL
Yao Di et al. (2025) β€” Degradation and ring-opening polymerization of poly(Ξ΅-caprolactone) by a novel enzyme from Pseudomonas sp. DS0801
iScience  Β· doi:10.1016/j.isci.2025.114173 β†—  Β· Activity - Classical
6 measurements ▼
Database ID
NCBI: PX234425.1 β†—
Sequence Annotation
Explicit - Provided PDB Accession Number (1 mutation)
Protein Source
Purified, bacterium Pseudomonas sp. DS0801

Experimental Data (6 measurements)

6 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (reaction mixture turbidity absorbance measurement at 650 nm) in the presence of organic solvent Ethanol 1% 100.0 95.6 % ? Buffer, Plysurf A210G for substrate solubilization ? 40Β°C 0.1 % (w/v) Polycaprolactone Polycaprolactone hydrolysis reaction products β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (reaction mixture turbidity absorbance measurement at 650 nm) in the presence of organic solvent Methanol 1% 100.0 96.2 % ? Buffer, Plysurf A210G for substrate solubilization ? 40Β°C 0.1 % (w/v) Polycaprolactone Polycaprolactone hydrolysis reaction products β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (reaction mixture turbidity absorbance measurement at 650 nm) in the presence of organic solvent Glycerol 1% 100.0 97.9 % ? Buffer, Plysurf A210G for substrate solubilization ? 40Β°C 0.1 % (w/v) Polycaprolactone Polycaprolactone hydrolysis reaction products β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (reaction mixture turbidity absorbance measurement at 650 nm) in the presence of organic solvent Ethanol 10% 100.0 68.2 % ? Buffer, Plysurf A210G for substrate solubilization ? 40Β°C 0.1 % (w/v) Polycaprolactone Polycaprolactone hydrolysis reaction products β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (reaction mixture turbidity absorbance measurement at 650 nm) in the presence of organic solvent Methanol 10% 100.0 82.3 % ? Buffer, Plysurf A210G for substrate solubilization ? 40Β°C 0.1 % (w/v) Polycaprolactone Polycaprolactone hydrolysis reaction products β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (reaction mixture turbidity absorbance measurement at 650 nm) in the presence of organic solvent Glycerol 10% 100.0 78.4 % ? Buffer, Plysurf A210G for substrate solubilization ? 40Β°C 0.1 % (w/v) Polycaprolactone Polycaprolactone hydrolysis reaction products β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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