Esterase (gene E34Tt) from Thermus thermophilus HB27

Enzyme Description

Species

Thermus thermophilus HB27 ↗

Extremophile

Yes "thermophilic" organism

EC Number

3.1.1.1 ↗

Sequence

Length: 329 amino acids

MKRLIALVLLFALALAQGLEAFWKAVEVPGGVCADGSPYRFYVSPGDPRKVVVDFQGGGACWDQATCGPESRTYRKRVDVQELYLAQGIYNRMSVANPFFGWTHVFVPYCTGDLHVGRATVDYGGFKVHHQGARNAQAALEYVFRNHTNPERVFVTGCSAGAYGAVLWADKILATYKNAQIAVCGDAGVGVVTEDFPGFTAWNPRLPELPGLSSPPKVSEIYRALAQAYPKAVLAQYTTLLDGTQIYFYALMKKEAAPSEATAREWAVAAERAVGFPASEANYTYYLAPGSQHCILPRPELYTLKVGEVSFLDWLRALAEGRTPPRVRP

Roberto González-González et al. (2022) — Reactivity of a Recombinant Esterase from Thermus thermophilus HB27 in Aqueous and Organic Media

Microorganisms · doi:10.3390/microorganisms10050915 ↗ · Activity - Classical

4 measurements ▼

Database ID

UniProt: Q72J75 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host yeast Kluyveromyces lactis NRRL-Y1140

Experimental Data (4 measurements)

4 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent	Methanol	10%	100.0	125.4	%	50 mM Tris-HCl buffer, 40 mM CaCl2	8	40°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100.0	104.5	%	50 mM Tris-HCl buffer, 40 mM CaCl2	8	40°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent	Ethanol	10%	100.0	80.9	%	50 mM Tris-HCl buffer, 40 mM CaCl2	8	40°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent	Isopropanol	10%	100.0	21.9	%	50 mM Tris-HCl buffer, 40 mM CaCl2	8	40°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent	Methanol	10%	100.0	125.4	%	50 mM Tris-HCl buffer, 40 mM CaCl2	8	40°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100.0	104.5	%	50 mM Tris-HCl buffer, 40 mM CaCl2	8	40°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent	Ethanol	10%	100.0	80.9	%	50 mM Tris-HCl buffer, 40 mM CaCl2	8	40°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 400 nm) in the presence of organic solvent	Isopropanol	10%	100.0	21.9	%	50 mM Tris-HCl buffer, 40 mM CaCl2	8	40°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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