Laccase DLac from Cerrena sp. RSD1

Enzyme Description

Species
Cerrena sp. RSD1 β†—
Extremophile
No
EC Number
1.10.3.2 β†—

Sequence

Length: 495 amino acids
AVGPVTDIHIVNKDIAPDGFSRPSVLAGGTFPGPLITGQKGDNFKLNVVDDLTDASMLKSTSIHWHGFFQKGTNWADGPAFVNQCPISTGNSFLYNFQVPDQAGTYWYHSHLSTQYCDGLRGAFVVYDPTDPHKALYDVDDESTVITLADWYHTLARQIVGVAIADTTLINGLGRNTNGPADAALAVINVEAGKRYRLRLVSISCDPNYVFSIDNHDFNIIEVDGVNSKPLNVDSIQIFAGQRYSAVLNANQPVGNYWVRANPNLGTTGFTGGINSAILRYKGAPVAEPTTTQTTSTKPLQEPNLRPLVSMPVPGSATPGGVDVVHNLILGFSAGKFTINGAAFTPPSVPVLLQILSGTTNAQDLLPSGSVITLPIGKTIELTLAAGVLGGPHPFHLHGHNFHVVRSAGQTTPNYVDPIVRDVVNTGGTGDNVTIRFTTDNPGPWFLHCHIDWHLEAGFAVVFAEGVNQTNAANPTPADWNNLCNIYNALADGDK
Meng-Hsuan Wu et al. (2019) β€” Enhancement of laccase activity by pre-incubation with organic solvents
Scientific Reports  Β· doi:10.1038/s41598-019-45118-x β†—  Β· Activity - Michaelis-Menten Activity + Stability - Incubation Stability - Tm
15 measurements ▼
Database ID
PDB: 5Z1X β†—
Sequence Annotation
Explicit - Provided PDB Accession Number
Protein Source
Purified, fungus Cerrena sp. RSD1

Experimental Data (15 measurements)

15 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Michaelis-Menten kcat (10^4 s^-1), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) Acetone 30% 3.56 13.33 10^4 * 1/s 40 mM sodium phosphate buffer, 80 mM citric acid 3 30Β°C ABTS ABTS radical cation reaction product β€” β€” Classical aqueous control (in 10^4 * 1/s)
Activity - Michaelis-Menten Km (Β΅M), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) Acetone 30% 24.0 45.0 Β΅M 40 mM sodium phosphate buffer, 80 mM citric acid 3 30Β°C ABTS ABTS radical cation reaction product β€” β€” Classical aqueous control (in microM)
Activity - Michaelis-Menten kcat/Km (10^3*s^-1*Β΅M^-1), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) Acetone 30% 1.47 2.98 10^3 * s^-1 * Β΅M^-1 40 mM sodium phosphate buffer, 80 mM citric acid 3 30Β°C ABTS ABTS radical cation reaction product β€” β€” Classical aqueous control (in 10^3 * s^-1 * microM^-1)
Activity + Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm), in the presence of organic solvents Acetone 30% 0.41 1.21 U/mL Incubation: 4 mM Tris-HCl, 30 mM NaCl, Assay: 40 mM sodium phosphate buffer, 80 mM citric acid Incubation: 8, Assay: 3 Incubation: 25Β°C, Assay: 30Β°C 0.3 mM ABTS ABTS radical cation reaction product β€” β€” Water-incubated control (in U/mL), assay in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm), in the presence of organic solvents Methanol 30% 0.41 1.27 U/mL Incubation: 4 mM Tris-HCl, 30 mM NaCl, Assay: 40 mM sodium phosphate buffer, 80 mM citric acid Incubation: 8, Assay: 3 Incubation: 25Β°C, Assay: 30Β°C 0.3 mM ABTS ABTS radical cation reaction product β€” β€” Water-incubated control (in U/mL), assay in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm), in the presence of organic solvents Ethanol 30% 0.41 1.24 U/mL Incubation: 4 mM Tris-HCl, 30 mM NaCl, Assay: 40 mM sodium phosphate buffer, 80 mM citric acid Incubation: 8, Assay: 3 Incubation: 25Β°C, Assay: 30Β°C 0.3 mM ABTS ABTS radical cation reaction product β€” β€” Water-incubated control (in U/mL), assay in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm), in the presence of organic solvents Dimethyl Sulfoxide (DMSO) 30% 0.41 1.27 U/mL Incubation: 4 mM Tris-HCl, 30 mM NaCl, Assay: 40 mM sodium phosphate buffer, 80 mM citric acid Incubation: 8, Assay: 3 Incubation: 25Β°C, Assay: 30Β°C 0.3 mM ABTS ABTS radical cation reaction product β€” β€” Water-incubated control (in U/mL), assay in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm), in the presence of organic solvents Dimethylformamide (DMF) 30% 0.41 1.26 U/mL Incubation: 4 mM Tris-HCl, 30 mM NaCl, Assay: 40 mM sodium phosphate buffer, 80 mM citric acid Incubation: 8, Assay: 3 Incubation: 25Β°C, Assay: 30Β°C 0.3 mM ABTS ABTS radical cation reaction product β€” β€” Water-incubated control (in U/mL), assay in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm), in the presence of organic solvents 1-Propanol 30% 0.41 0.57 U/mL Incubation: 4 mM Tris-HCl, 30 mM NaCl, Assay: 40 mM sodium phosphate buffer, 80 mM citric acid Incubation: 8, Assay: 3 Incubation: 25Β°C, Assay: 30Β°C 0.3 mM ABTS ABTS radical cation reaction product β€” β€” Water-incubated control (in U/mL), assay in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm), in the presence of organic solvents Acetonitrile 30% 0.41 0.08 U/mL Incubation: 4 mM Tris-HCl, 30 mM NaCl, Assay: 40 mM sodium phosphate buffer, 80 mM citric acid Incubation: 8, Assay: 3 Incubation: 25Β°C, Assay: 30Β°C 0.3 mM ABTS ABTS radical cation reaction product β€” β€” Water-incubated control (in U/mL), assay in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm), in the presence of organic solvents Formaldehyde 30% 0.41 0.11 U/mL Incubation: 4 mM Tris-HCl, 30 mM NaCl, Assay: 40 mM sodium phosphate buffer, 80 mM citric acid Incubation: 8, Assay: 3 Incubation: 25Β°C, Assay: 30Β°C 0.3 mM ABTS ABTS radical cation reaction product β€” β€” Water-incubated control (in U/mL), assay in the presence of organic solvent
Stability - Tm Temperature for which activity after incubation (10 minutes at various temperatures), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase, is half conserved Acetone 10% 63.5 59.7 Β°C Incubation: 4 mM Tris-HCl, 30 mM NaCl, Assay: 40 mM sodium phosphate buffer, 80 mM citric acid Incubation: 8, Assay: 3 β€” 0.3 mM ABTS ABTS radical cation reaction product β€” β€” Classical aqueous control (in Β°C)
Stability - Tm Temperature for which activity after incubation (10 minutes at various temperatures), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase, is half conserved Acetone 25% 63.5 57.9 Β°C Incubation: 4 mM Tris-HCl, 30 mM NaCl, Assay: 40 mM sodium phosphate buffer, 80 mM citric acid Incubation: 8, Assay: 3 β€” 0.3 mM ABTS ABTS radical cation reaction product β€” β€” Classical aqueous control (in Β°C)
Stability - Tm Temperature for which activity after incubation (10 minutes at various temperatures), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase, is half conserved Acetone 50% 63.5 51.1 Β°C Incubation: 4 mM Tris-HCl, 30 mM NaCl, Assay: 40 mM sodium phosphate buffer, 80 mM citric acid Incubation: 8, Assay: 3 β€” 0.3 mM ABTS ABTS radical cation reaction product β€” β€” Classical aqueous control (in Β°C)
Stability - Tm Temperature for which activity after incubation (10 minutes at various temperatures), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase, is half conserved Acetone 75% 63.5 38.3 Β°C Incubation: 4 mM Tris-HCl, 30 mM NaCl, Assay: 40 mM sodium phosphate buffer, 80 mM citric acid Incubation: 8, Assay: 3 β€” 0.3 mM ABTS ABTS radical cation reaction product β€” β€” Classical aqueous control (in Β°C)

Visualization : Activity β€” Michaelis-Menten

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability β€” Tm

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Activity + Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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