Cyclohexanone monooxygenase from Thermocrispum municipale
Enzyme Description
Species
Extremophile
Yes
thermophilic organism
EC Number
Sequence
MSTTQTPDLDAIVIGAGFGGIYMLHKLRNDLGLSVRVFEKGGGVGGTWYWNKYPGAKSDTEGFVYRYSFDKELLREYDWTTRYLDQPDVLAYLEHVVERYDLARDIQLNTEVTDAIFDEETELWRVTTAGGETLTARFLVTALGLLSRSNIPDIPGRDSFAGRLVHTNAWPEDLDITGKRVGVIGTGSTGTQFIVAAAKMAEQLTVFQRTPQYCVPSGNGPMDPDEVARIKQNFDSIWDQVRSSTVAFGFEESTVEAMSVSESERQRVFQQAWDKGNGFRFMFGTFCDIATNPEANAAAAAFIRSKIAEIVKDPETARKLTPTDLYAKRPLCNEGYYETYNRDNVSLVSLKETPIEEIVPQGVRTSDGVVHELDVLVFATGFDAVDGNYRAMNLRGRDGRHINEHWTEGPTSYLGVTKAGFPNMFMILGPNGPFTNLPPSIEAQVEWISDLIDKATREGLTTVEPTADAEREWTETCAEIANMTLFPKADSWIFGANIPGKRHAVMFYLGGLGNYRRQLADVADGGYRGFQLRGERAQAVA
Gonzalo de Gonzalo et al. (2018)
β Enantioselective sulfoxidations employing the thermostable cyclohexanone monooxygenase from Thermocrispum municipale
Enzyme and Microbial Technology
Β· doi:10.1016/j.enzmictec.2018.02.006 β
Β· Activity + Stability - Conversion
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Database ID
UniProt: A0A1L1QK40 β
Sequence Annotation
Inferred - from protein name
Protein Source
Recombinant, host bacterium Escherichia coli NEB 10-Ξ²
Experimental Data (5 measurements)
5 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity + Stability - Conversion | Conversion after incubation (48 hours at 17Β°C) in the presence of organic solvent, measured by gas chromatography | Methanol | 30% | 100 | 96 | % | 50 mM Tris-HCl buffer, 20 mM phosphite | 7 | 17Β°C | 11 mM 2-Butanone | Ethyl Acetate | 150 Β΅M NADPH | 200 rpm | Classical aqueous control (in %) |
| Activity + Stability - Conversion | Conversion after incubation (48 hours at 17Β°C) in the presence of organic solvent, measured by gas chromatography | Ethanol | 30% | 100 | 56 | % | 50 mM Tris-HCl buffer, 20 mM phosphite | 7 | 17Β°C | 11 mM 2-Butanone | Ethyl Acetate | 150 Β΅M NADPH | 200 rpm | Classical aqueous control (in %) |
| Activity + Stability - Conversion | Conversion after incubation (48 hours at 17Β°C) in the presence of organic solvent, measured by gas chromatography | 1,4-Dioxane | 15% | 100 | 56 | % | 50 mM Tris-HCl buffer, 20 mM phosphite | 7 | 17Β°C | 11 mM 2-Butanone | Ethyl Acetate | 150 Β΅M NADPH | 200 rpm | Classical aqueous control (in %) |
| Activity + Stability - Conversion | Conversion after incubation (48 hours at 17Β°C) in the presence of organic solvent, measured by gas chromatography | 1,3-Dioxane | 15% | 100 | 51 | % | 50 mM Tris-HCl buffer, 20 mM phosphite | 7 | 17Β°C | 11 mM 2-Butanone | Ethyl Acetate | 150 Β΅M NADPH | 200 rpm | Classical aqueous control (in %) |
| Activity + Stability - Conversion | Conversion after incubation (48 hours at 17Β°C) in the presence of organic solvent, measured by gas chromatography | 2-Methyl-1,3-Dioxolane | 15% | 100 | 25 | % | 50 mM Tris-HCl buffer, 20 mM phosphite | 7 | 17Β°C | 11 mM 2-Butanone | Ethyl Acetate | 150 Β΅M NADPH | 200 rpm | Classical aqueous control (in %) |
Visualization : Activity + Stability β Conversion
One bar per measurement. Colour = solvent, shade = solvent volume.