Imine reductase from Streptomyces turgidiscabies

Enzyme Description

Species

Extremophile

EC Number

1.5.1.20 ↗

Sequence

Length: 294 amino acids

MGDNHTSVSVIGLGLMGQALAAAFLKAGHATTVWNRSADKADGVVANGAVLAAAPADAVAASDLVVVCVSTYDVVHDVIGSLGDALRGKTVVNLTSGSSEQARQTAEWAEKNGARYLDGAIMITPPGIGTETAVLFYAGDQAVFDAHEPVLKLLGAGTTYLGTDHGKPALFDVSLLGLMWGALNSFLHGVAIETGGVKAQDFLPWAHMWLDAIKMFTADYAAQIDAGDEKFPANDATLETHLGALKHLVEESEALGVDTELPKYSEALMEGIIAQGHAKNSYASVVKAYRRPAR

Philipp N. Scheller et al. (2016) — The biochemical characterization of three imine-reducing enzymes from Streptosporangium roseum DSM43021, Streptomyces turgidiscabies and Paenibacillus elgii

Applied Microbiology and Biotechnology · doi:10.1007/s00253-016-7740-0 ↗ · Activity - Classical

40 measurements ▼

Database ID

NCBI: WP_006374254.1 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli JW5510

Experimental Data (40 measurements)

40 measurements — page 1 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	5%	100	42	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetonitrile	10%	100	11	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetonitrile	15%	100	3	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetonitrile	20%	100	4	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetonitrile	25%	100	0	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5%	100	32	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	22	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	15%	100	16	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	10	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	25%	100	5	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetonitrile	5%	100	33	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	10%	100	30	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	15%	100	24	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	20%	100	17	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	25%	100	9	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Glycerol	5%	100	44	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Glycerol	10%	100	39	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Glycerol	15%	100	54	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Glycerol	20%	100	47	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Glycerol	25%	100	36	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	5%	100	42	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetonitrile	10%	100	11	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetonitrile	15%	100	3	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetonitrile	20%	100	4	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetonitrile	25%	100	0	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5%	100	32	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	22	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	15%	100	16	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	10	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	25%	100	5	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetonitrile	5%	100	33	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	10%	100	30	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	15%	100	24	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	20%	100	17	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	25%	100	9	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Glycerol	5%	100	44	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Glycerol	10%	100	39	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Glycerol	15%	100	54	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Glycerol	20%	100	47	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Glycerol	25%	100	36	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetone	5%	100	21	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Isopropanol	10%	100	6	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Isopropanol	15%	100	3	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Isopropanol	20%	100	2	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Isopropanol	25%	100	0	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	tert-Butanol	5%	100	12	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	tert-Butanol	10%	100	4	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	tert-Butanol	15%	100	0	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	tert-Butanol	20%	100	0	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	tert-Butanol	25%	100	0	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Isopropanol	5%	100	13	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetone	10%	100	11	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetone	15%	100	4	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetone	20%	100	3	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetone	25%	100	1	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	5%	100	21	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	10%	100	9	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	15%	100	5	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	20%	100	5	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	25%	100	3	%	50 mM sodium phosphate buffer	7	30°C	5 mM 2-Methylpyrroline	2-Methylpyrrolidine	0.3 µM NADPH	—	Classical aqueous control (in %)

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Visualization : Activity — Classical

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