Imine reductase from Streptosporangium roseum DSM43021
Enzyme Description
Sequence
MRDTDVTVLGLGLMGQALAGAFLKDGHATTVWNRSEGKAGQLAEQGAVLASSARDAAEASPLVVVCVSDHAAVRAVLDPLGDVLAGRVLVNLTSGTSEQARATAEWAAERGITYLDGAIMAIPQVVGTADAFLLYSGPEAAYEAHEPTLRSLGAGTTYLGADHGLSSLYDVALLGIMWGTLNSFLHGAALLGTAKVEATTFAPFANRWIEAVTGFVSAYAGQVDQGAYPALDATIDTHVATVDHLIHESEAAGVNTELPRLVRTLADRALAGGQGGLGYAAMIEQFRSPSA
Philipp N. Scheller et al. (2016)
β The biochemical characterization of three imine-reducing enzymes from Streptosporangium roseum DSM43021, Streptomyces turgidiscabies and Paenibacillus elgii
Applied Microbiology and Biotechnology
Β· doi:10.1007/s00253-016-7740-0 β
Β· Activity - Classical
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Database ID
NCBI: WP_012887675.1 β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli JW5510
Experimental Data (40 measurements)
40 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent | Methanol | 5% | 100 | 48 | % | 50 mM sodium phosphate buffer | 7 | 30Β°C | 5 mM 2-Methylpyrroline | 2-Methylpyrrolidine | 0.3 Β΅M NADPH | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent | Acetonitrile | 10% | 100 | 6 | % | 50 mM sodium phosphate buffer | 7 | 30Β°C | 5 mM 2-Methylpyrroline | 2-Methylpyrrolidine | 0.3 Β΅M NADPH | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent | Acetonitrile | 15% | 100 | 0 | % | 50 mM sodium phosphate buffer | 7 | 30Β°C | 5 mM 2-Methylpyrroline | 2-Methylpyrrolidine | 0.3 Β΅M NADPH | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent | Acetonitrile | 20% | 100 | 0 | % | 50 mM sodium phosphate buffer | 7 | 30Β°C | 5 mM 2-Methylpyrroline | 2-Methylpyrrolidine | 0.3 Β΅M NADPH | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent | Acetonitrile | 25% | 100 | 0 | % | 50 mM sodium phosphate buffer | 7 | 30Β°C | 5 mM 2-Methylpyrroline | 2-Methylpyrrolidine | 0.3 Β΅M NADPH | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 5% | 100 | 43 | % | 50 mM sodium phosphate buffer | 7 | 30Β°C | 5 mM 2-Methylpyrroline | 2-Methylpyrrolidine | 0.3 Β΅M NADPH | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 10% | 100 | 32 | % | 50 mM sodium phosphate buffer | 7 | 30Β°C | 5 mM 2-Methylpyrroline | 2-Methylpyrrolidine | 0.3 Β΅M NADPH | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 15% | 100 | 18 | % | 50 mM sodium phosphate buffer | 7 | 30Β°C | 5 mM 2-Methylpyrroline | 2-Methylpyrrolidine | 0.3 Β΅M NADPH | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 20% | 100 | 11 | % | 50 mM sodium phosphate buffer | 7 | 30Β°C | 5 mM 2-Methylpyrroline | 2-Methylpyrrolidine | 0.3 Β΅M NADPH | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 25% | 100 | 7 | % | 50 mM sodium phosphate buffer | 7 | 30Β°C | 5 mM 2-Methylpyrroline | 2-Methylpyrrolidine | 0.3 Β΅M NADPH | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent | Acetonitrile | 5% | 100 | 25 | % | 50 mM sodium phosphate buffer | 7 | 30Β°C | 5 mM 2-Methylpyrroline | 2-Methylpyrrolidine | 0.3 Β΅M NADPH | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent | Methanol | 10% | 100 | 33 | % | 50 mM sodium phosphate buffer | 7 | 30Β°C | 5 mM 2-Methylpyrroline | 2-Methylpyrrolidine | 0.3 Β΅M NADPH | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent | Methanol | 15% | 100 | 17 | % | 50 mM sodium phosphate buffer | 7 | 30Β°C | 5 mM 2-Methylpyrroline | 2-Methylpyrrolidine | 0.3 Β΅M NADPH | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent | Methanol | 20% | 100 | 8 | % | 50 mM sodium phosphate buffer | 7 | 30Β°C | 5 mM 2-Methylpyrroline | 2-Methylpyrrolidine | 0.3 Β΅M NADPH | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent | Methanol | 25% | 100 | 2 | % | 50 mM sodium phosphate buffer | 7 | 30Β°C | 5 mM 2-Methylpyrroline | 2-Methylpyrrolidine | 0.3 Β΅M NADPH | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent | Glycerol | 5% | 100 | 61 | % | 50 mM sodium phosphate buffer | 7 | 30Β°C | 5 mM 2-Methylpyrroline | 2-Methylpyrrolidine | 0.3 Β΅M NADPH | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent | Glycerol | 10% | 100 | 53 | % | 50 mM sodium phosphate buffer | 7 | 30Β°C | 5 mM 2-Methylpyrroline | 2-Methylpyrrolidine | 0.3 Β΅M NADPH | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent | Glycerol | 15% | 100 | 36 | % | 50 mM sodium phosphate buffer | 7 | 30Β°C | 5 mM 2-Methylpyrroline | 2-Methylpyrrolidine | 0.3 Β΅M NADPH | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent | Glycerol | 20% | 100 | 35 | % | 50 mM sodium phosphate buffer | 7 | 30Β°C | 5 mM 2-Methylpyrroline | 2-Methylpyrrolidine | 0.3 Β΅M NADPH | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent | Glycerol | 25% | 100 | 16 | % | 50 mM sodium phosphate buffer | 7 | 30Β°C | 5 mM 2-Methylpyrroline | 2-Methylpyrrolidine | 0.3 Β΅M NADPH | β | Classical aqueous control (in %) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.