Chitinase (gene ChiA-Ba43) from Bacillus altitudinis KA15

Enzyme Description

Species
Bacillus altitudinis KA15 β†—
Extremophile
No but 'acido-thermostable" enzyme
EC Number
3.2.1.14 β†—

Sequence

Length: 409 amino acids
ASSQKSYKIIAYYPSWGYYGRDWQVWEMDVSKVSHINYAFAYICWEGRHGNPDPTGTNPQTWSCQNENGMIDAPNGTIVMGDPWIWAQKSNPGDVWDEPIRGNFKQLLKLKKSHPHLKTFISVGGWAWSNRFSDVAAQPAARENFAASAVEFLRKYGFDFVDLDWEYPVSGGLPGNSTRPEDKRNYTLLLQEVRKKLDVAEAKDGKEYLLTIASGASQDYVSNTELDKIAQTVDWINIMTYDFNGGWQSISAHNAPLFYDPEYKEAWIPSSAPVNVRVTGKTATSVSLAWDAPSSGANIAEYLVSFENRSISVKETSAEIGGLKPGTYYSFTVSAKDADGKLHAGPTVEVEENSDQACSYDEWKETSAYTGGERVRFNGKVYEAKWWTKGDRPDQSGWWAVWRLIGGCE
Katia-Louiza Asmani et al. (2020) β€” Biochemical and molecular characterization of an acido-thermostable endo-chitinase from Bacillus altitudinis KA15 for industrial degradation of chitinous waste
Carbohydrate Research  Β· doi:10.1016/j.carres.2020.108089 β†—  Β· Stability - Incubation
20 measurements ▼
Database ID
UniProt: A0A7H1K1N9 β†—
Sequence Annotation
Explicit - Provided (34 first residues from UniProt sequence absent from the mature protein)
Protein Source
Recombinant, host bacterium Escherichia coli HB101

Experimental Data (20 measurements)

20 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (24 hours at 50Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase 1-Hexanol 25% 100.0 105.1 % Incubation: ?, Assay: 50 mM MES buffer, 0.5 MM CaCl2 Incubation: ?, Assay: 4 Incubation: 50Β°C, Assay: 85Β°C 5 mg/ml Colloidal Chitin Reducing sugars β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 50Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 25% 100.0 125.0 % Incubation: ?, Assay: 50 mM MES buffer, 0.5 MM CaCl2 Incubation: ?, Assay: 4 Incubation: 50Β°C, Assay: 85Β°C 5 mg/ml Colloidal Chitin Reducing sugars β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 50Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Dimethylformamide (DMF) 25% 100.0 150.0 % Incubation: ?, Assay: 50 mM MES buffer, 0.5 MM CaCl2 Incubation: ?, Assay: 4 Incubation: 50Β°C, Assay: 85Β°C 5 mg/ml Colloidal Chitin Reducing sugars β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 50Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Methanol 25% 100.0 100.5 % Incubation: ?, Assay: 50 mM MES buffer, 0.5 MM CaCl2 Incubation: ?, Assay: 4 Incubation: 50Β°C, Assay: 85Β°C 5 mg/ml Colloidal Chitin Reducing sugars β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 50Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Ethanol 25% 100.0 107.1 % Incubation: ?, Assay: 50 mM MES buffer, 0.5 MM CaCl2 Incubation: ?, Assay: 4 Incubation: 50Β°C, Assay: 85Β°C 5 mg/ml Colloidal Chitin Reducing sugars β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 50Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Acetonitrile 25% 100.0 0.0 % Incubation: ?, Assay: 50 mM MES buffer, 0.5 MM CaCl2 Incubation: ?, Assay: 4 Incubation: 50Β°C, Assay: 85Β°C 5 mg/ml Colloidal Chitin Reducing sugars β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 50Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Isopropanol 25% 100.0 92.8 % Incubation: ?, Assay: 50 mM MES buffer, 0.5 MM CaCl2 Incubation: ?, Assay: 4 Incubation: 50Β°C, Assay: 85Β°C 5 mg/ml Colloidal Chitin Reducing sugars β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 50Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Isopropanol 25% 100.0 119.9 % Incubation: ?, Assay: 50 mM MES buffer, 0.5 MM CaCl2 Incubation: ?, Assay: 4 Incubation: 50Β°C, Assay: 85Β°C 5 mg/ml Colloidal Chitin Reducing sugars β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 50Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Ethyl Acetate 25% 100.0 0.0 % Incubation: ?, Assay: 50 mM MES buffer, 0.5 MM CaCl2 Incubation: ?, Assay: 4 Incubation: 50Β°C, Assay: 85Β°C 5 mg/ml Colloidal Chitin Reducing sugars β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 50Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase 1-Butanol 25% 100.0 88.8 % Incubation: ?, Assay: 50 mM MES buffer, 0.5 MM CaCl2 Incubation: ?, Assay: 4 Incubation: 50Β°C, Assay: 85Β°C 5 mg/ml Colloidal Chitin Reducing sugars β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 50Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase n-Hexadecane 25% 100.0 88.3 % Incubation: ?, Assay: 50 mM MES buffer, 0.5 MM CaCl2 Incubation: ?, Assay: 4 Incubation: 50Β°C, Assay: 85Β°C 5 mg/ml Colloidal Chitin Reducing sugars β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 50Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Chloroform 25% 100.0 189.8 % Incubation: ?, Assay: 50 mM MES buffer, 0.5 MM CaCl2 Incubation: ?, Assay: 4 Incubation: 50Β°C, Assay: 85Β°C 5 mg/ml Colloidal Chitin Reducing sugars β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 50Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Benzene 25% 100.0 66.3 % Incubation: ?, Assay: 50 mM MES buffer, 0.5 MM CaCl2 Incubation: ?, Assay: 4 Incubation: 50Β°C, Assay: 85Β°C 5 mg/ml Colloidal Chitin Reducing sugars β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 50Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Toluene 25% 100.0 70.9 % Incubation: ?, Assay: 50 mM MES buffer, 0.5 MM CaCl2 Incubation: ?, Assay: 4 Incubation: 50Β°C, Assay: 85Β°C 5 mg/ml Colloidal Chitin Reducing sugars β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 50Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Cyclohexane 25% 100.0 164.8 % Incubation: ?, Assay: 50 mM MES buffer, 0.5 MM CaCl2 Incubation: ?, Assay: 4 Incubation: 50Β°C, Assay: 85Β°C 5 mg/ml Colloidal Chitin Reducing sugars β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 50Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase n-Hexane 25% 100.0 160.2 % Incubation: ?, Assay: 50 mM MES buffer, 0.5 MM CaCl2 Incubation: ?, Assay: 4 Incubation: 50Β°C, Assay: 85Β°C 5 mg/ml Colloidal Chitin Reducing sugars β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 50Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase n-Heptane 25% 100.0 109.7 % Incubation: ?, Assay: 50 mM MES buffer, 0.5 MM CaCl2 Incubation: ?, Assay: 4 Incubation: 50Β°C, Assay: 85Β°C 5 mg/ml Colloidal Chitin Reducing sugars β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 50Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase n-Octane 25% 100.0 99.0 % Incubation: ?, Assay: 50 mM MES buffer, 0.5 MM CaCl2 Incubation: ?, Assay: 4 Incubation: 50Β°C, Assay: 85Β°C 5 mg/ml Colloidal Chitin Reducing sugars β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 50Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Isooctane 25% 100.0 101.0 % Incubation: ?, Assay: 50 mM MES buffer, 0.5 MM CaCl2 Incubation: ?, Assay: 4 Incubation: 50Β°C, Assay: 85Β°C 5 mg/ml Colloidal Chitin Reducing sugars β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 50Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase n-Decane 25% 100.0 79.1 % Incubation: ?, Assay: 50 mM MES buffer, 0.5 MM CaCl2 Incubation: ?, Assay: 4 Incubation: 50Β°C, Assay: 85Β°C 5 mg/ml Colloidal Chitin Reducing sugars β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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