Flavin reductase (gene MoxB) from a deep-sea sediment metagenomic library

Enzyme Description

Species

Na (deep-sea sediment metagenomic library)

Extremophile

EC Number

1.5.1.29 ↗

Sequence

Length: 310 amino acids

MSSAEPFDAFKFRNALGNFATGVTVVTAKDTNNGYVGTTASSFNSVSMDPPLILWSIDKGARALESYEQAEHFVVNVLAADQVSVSNHFARQQDDKFKDMDYSLSEVGVPVLSGCSAVFECKTAFQYEGGDHIIIVGEVLNFKTTDRNALLFHNGAYAVSEYHPVSSDRMISEEQKDESFAENYLPSLIGRSFYQLLAKLLPFLERQGIEENEYRVLASIAGSPGRTPEELQNYTVLQGAQLDKAVTALLERGLIADKDSLIMLTEKGQETIDPLIRLAKSNEADTLGHFNVEEALLLKSYLKRMVNWLS

Jikun Du et al. (2024) — Functional characterization of a novel flavin reductase from a deep-sea sediment metagenomic library and its application for indirubin production

Applied and Environmental Microbiology · doi:10.1128/aem.00429-24 ↗ · Activity - Classical

6 measurements ▼

Database ID

UniParc: UPI00016529B5 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21

Experimental Data (6 measurements)

6 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	1%	100.0	90.8	%	100 mM Tris-HCl buffer	8	37°C	10 µM FAD, 500 µM NADH	FADH2 , NAD+	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5%	100.0	81.9	%	100 mM Tris-HCl buffer	8	37°C	10 µM FAD, 500 µM NADH	FADH2 , NAD+	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	1%	100.0	89.1	%	100 mM Tris-HCl buffer	8	37°C	10 µM FAD, 500 µM NADH	FADH2 , NAD+	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	5%	100.0	75.1	%	100 mM Tris-HCl buffer	8	37°C	10 µM FAD, 500 µM NADH	FADH2 , NAD+	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	1%	100.0	92.6	%	100 mM Tris-HCl buffer	8	37°C	10 µM FAD, 500 µM NADH	FADH2 , NAD+	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	5%	100.0	78.5	%	100 mM Tris-HCl buffer	8	37°C	10 µM FAD, 500 µM NADH	FADH2 , NAD+	—	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	1%	100.0	90.8	%	100 mM Tris-HCl buffer	8	37°C	10 µM FAD, 500 µM NADH	FADH2, NAD+	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5%	100.0	81.9	%	100 mM Tris-HCl buffer	8	37°C	10 µM FAD, 500 µM NADH	FADH2, NAD+	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	1%	100.0	89.1	%	100 mM Tris-HCl buffer	8	37°C	10 µM FAD, 500 µM NADH	FADH2, NAD+	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	5%	100.0	75.1	%	100 mM Tris-HCl buffer	8	37°C	10 µM FAD, 500 µM NADH	FADH2, NAD+	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	1%	100.0	92.6	%	100 mM Tris-HCl buffer	8	37°C	10 µM FAD, 500 µM NADH	FADH2, NAD+	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	5%	100.0	78.5	%	100 mM Tris-HCl buffer	8	37°C	10 µM FAD, 500 µM NADH	FADH2, NAD+	—	—	Classical aqueous control (in %)

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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