Creatine kinase from Oryctolagus cuniculus (rabbit)
Enzyme Description
Sequence
MPFGNTHNKYKLNYKSEEEYPDLSKHNNHMAKVLTPDLYKKLRDKETPSGFTLDDVIQTGVDNPGHPFIMTVGCVAGDEESYTVFKDLFDPIIQDRHGGFKPTDKHKTDLNHENLKGGDDLDPHYVLSSRVRTGRSIKGYTLPPHCSRGERRAVEKLSVEALNSLTGEFKGKYYPLKSMTEQEQQQLIDDHFLFDKPVSPLLLASGMARDWPDARGIWHNDNKSFLVWVNEEDHLRVISMEKGGNMKEVFRRFCVGLQKIEEIFKKAGHPFMWNEHLGYVLTCPSNLGTGLRGGVHVKLAHLSKHPKFEEILTRLRLQKRGTGGVDTAAVGSVFDISNADRLGSSEVEQVQLVVDGVKLMVEMEKKLEKGQSIDDMIPAQK
Wen-bin Ou et al. (2002)
β Conformational changes and inactivation of rabbit muscle creatine kinase in dimethyl sulfoxide solutions
Biochemistry and Cell Biology
Β· doi:10.1139/o02-132 β
Β· Stability - Incubation
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Database ID
UniProt: P00563 β
Sequence Annotation
Inferred - from protein name
Protein Source
Purified, eukaryote Oryctolagus cuniculus (rabbit)
Experimental Data (14 measurements)
14 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (4 hours at 25Β°C) measured by absorbance spectrophotometry (pH colorimetry method, thymol blue indicator abosrbance change by H+ released from reaction absorbance measurement, 597 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 2.5% | 100 | 99 | % | Incubation: 30 mM Tris-HCl, Assay: 5 mM Gly-NaOH buffer, 5 mM Mg2+, 0.01% Thymol Blue (w/v) | Incubation: 8, Assay: 9 | Incubation: 25Β°C, Assay: 25Β°C | ATP (Adenosine triphosphate), 24 mM Creatine | ADP , H+ , Phosphocreatine | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (4 hours at 25Β°C) measured by absorbance spectrophotometry (pH colorimetry method, thymol blue indicator abosrbance change by H+ released from reaction absorbance measurement, 597 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 5% | 100 | 98 | % | Incubation: 30 mM Tris-HCl, Assay: 5 mM Gly-NaOH buffer, 5 mM Mg2+, 0.01% Thymol Blue (w/v) | Incubation: 8, Assay: 9 | Incubation: 25Β°C, Assay: 25Β°C | ATP (Adenosine triphosphate), 24 mM Creatine | ADP , H+ , Phosphocreatine | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (4 hours at 25Β°C) measured by absorbance spectrophotometry (pH colorimetry method, thymol blue indicator abosrbance change by H+ released from reaction absorbance measurement, 597 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 20% | 100 | 97 | % | Incubation: 30 mM Tris-HCl, Assay: 5 mM Gly-NaOH buffer, 5 mM Mg2+, 0.01% Thymol Blue (w/v) | Incubation: 8, Assay: 9 | Incubation: 25Β°C, Assay: 25Β°C | ATP (Adenosine triphosphate), 24 mM Creatine | ADP , H+ , Phosphocreatine | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (4 hours at 25Β°C) measured by absorbance spectrophotometry (pH colorimetry method, thymol blue indicator abosrbance change by H+ released from reaction absorbance measurement, 597 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 30% | 100 | 97 | % | Incubation: 30 mM Tris-HCl, Assay: 5 mM Gly-NaOH buffer, 5 mM Mg2+, 0.01% Thymol Blue (w/v) | Incubation: 8, Assay: 9 | Incubation: 25Β°C, Assay: 25Β°C | ATP (Adenosine triphosphate), 24 mM Creatine | ADP , H+ , Phosphocreatine | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (4 hours at 25Β°C) measured by absorbance spectrophotometry (pH colorimetry method, thymol blue indicator abosrbance change by H+ released from reaction absorbance measurement, 597 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 40% | 100 | 97 | % | Incubation: 30 mM Tris-HCl, Assay: 5 mM Gly-NaOH buffer, 5 mM Mg2+, 0.01% Thymol Blue (w/v) | Incubation: 8, Assay: 9 | Incubation: 25Β°C, Assay: 25Β°C | ATP (Adenosine triphosphate), 24 mM Creatine | ADP , H+ , Phosphocreatine | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (4 hours at 25Β°C) measured by absorbance spectrophotometry (pH colorimetry method, thymol blue indicator abosrbance change by H+ released from reaction absorbance measurement, 597 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 50% | 100 | 96 | % | Incubation: 30 mM Tris-HCl, Assay: 5 mM Gly-NaOH buffer, 5 mM Mg2+, 0.01% Thymol Blue (w/v) | Incubation: 8, Assay: 9 | Incubation: 25Β°C, Assay: 25Β°C | ATP (Adenosine triphosphate), 24 mM Creatine | ADP , H+ , Phosphocreatine | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (4 hours at 25Β°C) measured by absorbance spectrophotometry (pH colorimetry method, thymol blue indicator abosrbance change by H+ released from reaction absorbance measurement, 597 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 60% | 100 | 96 | % | Incubation: 30 mM Tris-HCl, Assay: 5 mM Gly-NaOH buffer, 5 mM Mg2+, 0.01% Thymol Blue (w/v) | Incubation: 8, Assay: 9 | Incubation: 25Β°C, Assay: 25Β°C | ATP (Adenosine triphosphate), 24 mM Creatine | ADP , H+ , Phosphocreatine | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (4 hours at 25Β°C) measured by absorbance spectrophotometry (pH colorimetry method, thymol blue indicator abosrbance change by H+ released from reaction absorbance measurement, 597 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 65% | 100 | 96 | % | Incubation: 30 mM Tris-HCl, Assay: 5 mM Gly-NaOH buffer, 5 mM Mg2+, 0.01% Thymol Blue (w/v) | Incubation: 8, Assay: 9 | Incubation: 25Β°C, Assay: 25Β°C | ATP (Adenosine triphosphate), 24 mM Creatine | ADP , H+ , Phosphocreatine | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (4 hours at 25Β°C) measured by absorbance spectrophotometry (pH colorimetry method, thymol blue indicator abosrbance change by H+ released from reaction absorbance measurement, 597 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 70% | 100 | 76 | % | Incubation: 30 mM Tris-HCl, Assay: 5 mM Gly-NaOH buffer, 5 mM Mg2+, 0.01% Thymol Blue (w/v) | Incubation: 8, Assay: 9 | Incubation: 25Β°C, Assay: 25Β°C | ATP (Adenosine triphosphate), 24 mM Creatine | ADP , H+ , Phosphocreatine | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (4 hours at 25Β°C) measured by absorbance spectrophotometry (pH colorimetry method, thymol blue indicator abosrbance change by H+ released from reaction absorbance measurement, 597 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 71% | 100 | 44 | % | Incubation: 30 mM Tris-HCl, Assay: 5 mM Gly-NaOH buffer, 5 mM Mg2+, 0.01% Thymol Blue (w/v) | Incubation: 8, Assay: 9 | Incubation: 25Β°C, Assay: 25Β°C | ATP (Adenosine triphosphate), 24 mM Creatine | ADP , H+ , Phosphocreatine | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (4 hours at 25Β°C) measured by absorbance spectrophotometry (pH colorimetry method, thymol blue indicator abosrbance change by H+ released from reaction absorbance measurement, 597 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 72.5% | 100 | 11 | % | Incubation: 30 mM Tris-HCl, Assay: 5 mM Gly-NaOH buffer, 5 mM Mg2+, 0.01% Thymol Blue (w/v) | Incubation: 8, Assay: 9 | Incubation: 25Β°C, Assay: 25Β°C | ATP (Adenosine triphosphate), 24 mM Creatine | ADP , H+ , Phosphocreatine | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (4 hours at 25Β°C) measured by absorbance spectrophotometry (pH colorimetry method, thymol blue indicator abosrbance change by H+ released from reaction absorbance measurement, 597 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 75% | 100 | 8 | % | Incubation: 30 mM Tris-HCl, Assay: 5 mM Gly-NaOH buffer, 5 mM Mg2+, 0.01% Thymol Blue (w/v) | Incubation: 8, Assay: 9 | Incubation: 25Β°C, Assay: 25Β°C | ATP (Adenosine triphosphate), 24 mM Creatine | ADP , H+ , Phosphocreatine | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (4 hours at 25Β°C) measured by absorbance spectrophotometry (pH colorimetry method, thymol blue indicator abosrbance change by H+ released from reaction absorbance measurement, 597 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 80% | 100 | 8 | % | Incubation: 30 mM Tris-HCl, Assay: 5 mM Gly-NaOH buffer, 5 mM Mg2+, 0.01% Thymol Blue (w/v) | Incubation: 8, Assay: 9 | Incubation: 25Β°C, Assay: 25Β°C | ATP (Adenosine triphosphate), 24 mM Creatine | ADP , H+ , Phosphocreatine | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (4 hours at 25Β°C) measured by absorbance spectrophotometry (pH colorimetry method, thymol blue indicator abosrbance change by H+ released from reaction absorbance measurement, 597 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 90% | 100 | 6 | % | Incubation: 30 mM Tris-HCl, Assay: 5 mM Gly-NaOH buffer, 5 mM Mg2+, 0.01% Thymol Blue (w/v) | Incubation: 8, Assay: 9 | Incubation: 25Β°C, Assay: 25Β°C | ATP (Adenosine triphosphate), 24 mM Creatine | ADP , H+ , Phosphocreatine | β | β | Non-incubated control (in %), assay in aqueous phase |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.