Cytochrome P450 CYP107PQ1 from Meiothermus ruber

Enzyme Description

Species
Meiothermus ruber β†—
Extremophile
Yes "thermophilic" organism
EC Number
1.11.2.- β†—

Sequence

Length: 429 amino acids
GGSSHHHHHHSSGENLYFQGHMHSYHLNINDPAFIYDPYPTLAHLRENLPVFYDEVWNKIFFLRYEDIANLLRDKRLGRSITHILSRDELGWPPPNPLTRDFDHFQENHMLDNEPPKHTRLKGLMMKAFTPARVEGLRGKIQAIVNELLDRAEDLGQMDLLRDYAEPLPVTVIAELLGVPKEDRHLLRPWSAKIVKLYELGFTDEQAREANQAVVEFSAYIRQLADERRRKPGDDLISALVEVEEQGDKLTPDELVANCILLLNAGHEATVNGTTAGFLALSRNPEQMEQAKEAAAKNHPEFFKLAVEELLRYDTPLPMFERWVLEDFEYKGIPLRRGQEVALMYASGNRDPRKFPDPDRLCLTRTENYHLTFGLGIHYCIGAPLARLELQTSFQTLLKRLPNIHLATDRIEYAGGFVIRGHKAMPVAF
Tuhin Das et al. (2024) β€” A Thermostable Heme Protein Fold Adapted for Stereoselective Cβˆ’H Bond Hydroxylation Using Peroxygenase Activity
ChemBioChem  Β· doi:10.1002/cbic.202400737 β†—  Β· Activity + Stability - Product Formation
16 measurements ▼
Database ID
β€”
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (16 measurements)

16 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity + Stability - Product Formation Relative product formation after 2 hours incubation at 30Β°C, measued by HPLC in the presence of organic solvent Acetonitrile 10% 100.0 54.1 % 50 mM Tris-HCl buffer 7.5 30Β°C 1 mM Ξ²-Ionone, Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) (S)-4-hydroxy-beta-Ionone , H2O β€” Yes, "Reaction conducted in a shaking incubator" Classical aqueous control (in %)
Activity + Stability - Product Formation Relative product formation after 2 hours incubation at 30Β°C, measued by HPLC in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10% 100.0 98.5 % 50 mM Tris-HCl buffer 7.5 30Β°C 1 mM Ξ²-Ionone, Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) (S)-4-hydroxy-beta-Ionone , H2O β€” Yes, "Reaction conducted in a shaking incubator" Classical aqueous control (in %)
Activity + Stability - Product Formation Relative product formation after 2 hours incubation at 30Β°C, measued by HPLC in the presence of organic solvent Ethanol 10% 100.0 60.7 % 50 mM Tris-HCl buffer 7.5 30Β°C 1 mM Ξ²-Ionone, Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) (S)-4-hydroxy-beta-Ionone , H2O β€” Yes, "Reaction conducted in a shaking incubator" Classical aqueous control (in %)
Activity + Stability - Product Formation Relative product formation after 2 hours incubation at 30Β°C, measued by HPLC in the presence of organic solvent Isopropanol 10% 100.0 78.5 % 50 mM Tris-HCl buffer 7.5 30Β°C 1 mM Ξ²-Ionone, Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) (S)-4-hydroxy-beta-Ionone , H2O β€” Yes, "Reaction conducted in a shaking incubator" Classical aqueous control (in %)
Activity + Stability - Product Formation Relative product formation after 2 hours incubation at 30Β°C, measued by HPLC in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 5% 2.0 2.22 relative units, control equal 2 50 mM Tris-HCl buffer 7.5 30Β°C 1 mM Ξ²-Ionone, Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) (S)-4-hydroxy-beta-Ionone , H2O β€” Yes, "Reaction conducted in a shaking incubator" Classical aqueous control (in relative units, control equal 2)
Activity + Stability - Product Formation Relative product formation after 2 hours incubation at 30Β°C, measued by HPLC in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10% 2.0 2.39 relative units, control equal 2 50 mM Tris-HCl buffer 7.5 30Β°C 1 mM Ξ²-Ionone, Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) (S)-4-hydroxy-beta-Ionone , H2O β€” Yes, "Reaction conducted in a shaking incubator" Classical aqueous control (in relative units, control equal 2)
Activity + Stability - Product Formation Relative product formation after 2 hours incubation at 30Β°C, measued by HPLC in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 20% 2.0 2.56 relative units, control equal 2 50 mM Tris-HCl buffer 7.5 30Β°C 1 mM Ξ²-Ionone, Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) (S)-4-hydroxy-beta-Ionone , H2O β€” Yes, "Reaction conducted in a shaking incubator" Classical aqueous control (in relative units, control equal 2)
Activity + Stability - Product Formation Relative product formation after 2 hours incubation at 30Β°C, measued by HPLC in the presence of organic solvent Isopropanol 5% 2.0 2.7 relative units, control equal 2 50 mM Tris-HCl buffer 7.5 30Β°C 1 mM Ξ²-Ionone, Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) (S)-4-hydroxy-beta-Ionone , H2O β€” Yes, "Reaction conducted in a shaking incubator" Classical aqueous control (in relative units, control equal 2)
Activity + Stability - Product Formation Relative product formation after 2 hours incubation at 30Β°C, measued by HPLC in the presence of organic solvent Isopropanol 10% 2.0 2.81 relative units, control equal 2 50 mM Tris-HCl buffer 7.5 30Β°C 1 mM Ξ²-Ionone, Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) (S)-4-hydroxy-beta-Ionone , H2O β€” Yes, "Reaction conducted in a shaking incubator" Classical aqueous control (in relative units, control equal 2)
Activity + Stability - Product Formation Relative product formation after 2 hours incubation at 30Β°C, measued by HPLC in the presence of organic solvent Isopropanol 20% 2.0 2.37 relative units, control equal 2 50 mM Tris-HCl buffer 7.5 30Β°C 1 mM Ξ²-Ionone, Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) (S)-4-hydroxy-beta-Ionone , H2O β€” Yes, "Reaction conducted in a shaking incubator" Classical aqueous control (in relative units, control equal 2)
Activity + Stability - Product Formation Relative product formation after 2 hours incubation at 30Β°C, measued by HPLC in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 5% 100.0 110.9 % 50 mM Tris-HCl buffer 7.5 30Β°C 2 mM Ξ²-Ionone, Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) (S)-4-hydroxy-beta-Ionone , H2O β€” Yes, "Reaction conducted in a shaking incubator" Classical aqueous control (in %)
Activity + Stability - Product Formation Relative product formation after 2 hours incubation at 30Β°C, measued by HPLC in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10% 100.0 109.0 % 50 mM Tris-HCl buffer 7.5 30Β°C 2 mM Ξ²-Ionone, Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) (S)-4-hydroxy-beta-Ionone , H2O β€” Yes, "Reaction conducted in a shaking incubator" Classical aqueous control (in %)
Activity + Stability - Product Formation Relative product formation after 2 hours incubation at 30Β°C, measued by HPLC in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 20% 100.0 117.5 % 50 mM Tris-HCl buffer 7.5 30Β°C 2 mM Ξ²-Ionone, Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) (S)-4-hydroxy-beta-Ionone , H2O β€” Yes, "Reaction conducted in a shaking incubator" Classical aqueous control (in %)
Activity + Stability - Product Formation Relative product formation after 2 hours incubation at 30Β°C, measued by HPLC in the presence of organic solvent Isopropanol 5% 100.0 123.8 % 50 mM Tris-HCl buffer 7.5 30Β°C 2 mM Ξ²-Ionone, Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) (S)-4-hydroxy-beta-Ionone , H2O β€” Yes, "Reaction conducted in a shaking incubator" Classical aqueous control (in %)
Activity + Stability - Product Formation Relative product formation after 2 hours incubation at 30Β°C, measued by HPLC in the presence of organic solvent Isopropanol 10% 100.0 130.9 % 50 mM Tris-HCl buffer 7.5 30Β°C 2 mM Ξ²-Ionone, Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) (S)-4-hydroxy-beta-Ionone , H2O β€” Yes, "Reaction conducted in a shaking incubator" Classical aqueous control (in %)
Activity + Stability - Product Formation Relative product formation after 2 hours incubation at 30Β°C, measued by HPLC in the presence of organic solvent Isopropanol 20% 100.0 111.4 % 50 mM Tris-HCl buffer 7.5 30Β°C 2 mM Ξ²-Ionone, Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) (S)-4-hydroxy-beta-Ionone , H2O β€” Yes, "Reaction conducted in a shaking incubator" Classical aqueous control (in %)

Visualization : Activity + Stability β€” Product Formation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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