Lipase Ab3 from Sphingobacterium sp.
Enzyme Description
Species
Extremophile
No
but "psychrotolerant" organism
EC Number
Sequence
MNTIDQELLAVISRSAYGEIDYETLLQHNPQKIREQEMQVSLLEADTIIPPQLDIQNRYIPSSDKGRNIRLRTYRSKEKSNLPILLYFHGGAFIYGTPEQYDFIFFRLASDLDLMIVSVDYRLAPEHPFPAAMEDGYDSLKWLVHHASQLGGDPNRVLIGGSSAGATIAASITHLARDKQEIKIRHQLLLYPAMSHLLNTASMESLADAPMQTKNAAKWMWKHYLPHMDQRPAYAVPLLEENFNQLPDATVVVCEFDPLKDEGKMYAEKLRQAAVSTELIEIQGAVHAFDFFESSLSDAFYDRLLNVLTKTVNQDK
You Kiat Ng et al. (2022)
β Characterization of Sphingobacterium sp. Ab3 Lipase and Its Coexpression with LEA Peptides
Microbiology Spectrum
Β· doi:10.1128/spectrum.01422-21 β
Β· Activity - Classical
▼
Database ID
β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (4 measurements)
4 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity after incubation (30 minutes at 15Β°C), measured by absorbance spectrophotometry (Kwan-Rhee method, colorimetric assay with copper reacting wit the free fatty acid products, 715 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 30% | 100.0 | 101.8 | % | 36 mM sodium phosphate buffer, 0.36% polyvynil alcohol, 0.1 MM CaCl2 assay | 7 | 15Β°C | 36% Olive oil | free fatty acid , glycerol | β | Incubation: 180 rpm | Classical aqueous control (in %) | Short preincubation before assay in the presence of organic solvent (30 minutes), assay clearly in the presence of organic solvent |
| Activity - Classical | Activity after incubation (30 minutes at 15Β°C), measured by absorbance spectrophotometry (Kwan-Rhee method, colorimetric assay with copper reacting wit the free fatty acid products, 715 nm) in the presence of organic solvent | Methanol | 30% | 100.0 | 128.8 | % | 36 mM sodium phosphate buffer, 0.36% polyvynil alcohol, 0.1 MM CaCl2 assay | 7 | 15Β°C | 36% Olive oil | free fatty acid , glycerol | β | Incubation: 180 rpm | Classical aqueous control (in %) | Short preincubation before assay in the presence of organic solvent (30 minutes), assay clearly in the presence of organic solvent |
| Activity - Classical | Activity after incubation (30 minutes at 15Β°C), measured by absorbance spectrophotometry (Kwan-Rhee method, colorimetric assay with copper reacting wit the free fatty acid products, 715 nm) in the presence of organic solvent | Ethanol | 30% | 100.0 | 100.0 | % | 36 mM sodium phosphate buffer, 0.36% polyvynil alcohol, 0.1 MM CaCl2 assay | 7 | 15Β°C | 36% Olive oil | free fatty acid , glycerol | β | Incubation: 180 rpm | Classical aqueous control (in %) | Short preincubation before assay in the presence of organic solvent (30 minutes), assay clearly in the presence of organic solvent |
| Activity - Classical | Activity after incubation (30 minutes at 15Β°C), measured by absorbance spectrophotometry (Kwan-Rhee method, colorimetric assay with copper reacting wit the free fatty acid products, 715 nm) in the presence of organic solvent | Isopropanol | 30% | 100.0 | 75.6 | % | 36 mM sodium phosphate buffer, 0.36% polyvynil alcohol, 0.1 MM CaCl2 assay | 7 | 15Β°C | 36% Olive oil | free fatty acid , glycerol | β | Incubation: 180 rpm | Classical aqueous control (in %) | Short preincubation before assay in the presence of organic solvent (30 minutes), assay clearly in the presence of organic solvent |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.