Lipase (gene StSGNH1) from Sphaerobacter thermophilus
Enzyme Description
Sequence
HHHHHHSSGVDLGTENLYFQSNAMRRTRSALMVALLIATALAPLAARSTPRVAAQPALPEQVYLALGDSIAAGLVTSLPRVRGYPWLVRDLMEKHFTSEGAPAVTLINRAVPGETTTSFLAGDQLREARADIEAARARGAEVRAVTVTLGGNDILRLAGSDEAERQAGLVQFRSTFPAALAAIREALGEMPADIVVTTYYDLSEGNPAEEGSDAWWVAQFNEVIRQSAAAEGARVADVEPVFRGNIREWTWYPSDIHANNAGHAEIARLVWQALGYDQEPPTVTIERPAAGPLGRRTPTVRVRADDAVGVTRVELLVDGTYVQDLRYIPSQGAYLGVWDARDWSDASATLTVRATDLAGNQASAEVEVALPGS
Kelsey Minium et al. (2025)
β Characterization of a Highly Solvent-Tolerant SGNH Hydrolase Superfamily Lipolytic Enzyme from Sphaerobacter thermophilus
Biochemistry
Β· doi:10.1021/acs.biochem.5c00057 β
Β· Activity - Classical
▼
Database ID
β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (19 measurements)
19 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | 1-Propanol | 50% | 100 | 101 | % | 50 mM phosphate buffer, 1% Triton-X-100 | 8 | 25Β°C | 1 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Acetonitrile | 50% | 100 | 89 | % | 50 mM phosphate buffer, 1% Triton-X-100 | 8 | 25Β°C | 1 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Acetonitrile | 30% | 100 | 179 | % | 50 mM phosphate buffer, 1% Triton-X-100 | 8 | 25Β°C | 1 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Acetonitrile | 10% | 100 | 108 | % | 50 mM phosphate buffer, 1% Triton-X-100 | 8 | 25Β°C | 1 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Acetone | 50% | 100 | 10 | % | 50 mM phosphate buffer, 1% Triton-X-100 | 8 | 25Β°C | 1 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Acetone | 30% | 100 | 58 | % | 50 mM phosphate buffer, 1% Triton-X-100 | 8 | 25Β°C | 1 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Acetone | 10% | 100 | 121 | % | 50 mM phosphate buffer, 1% Triton-X-100 | 8 | 25Β°C | 1 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Isopropanol | 50% | 100 | 31 | % | 50 mM phosphate buffer, 1% Triton-X-100 | 8 | 25Β°C | 1 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Isopropanol | 30% | 100 | 188 | % | 50 mM phosphate buffer, 1% Triton-X-100 | 8 | 25Β°C | 1 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Isopropanol | 10% | 100 | 138 | % | 50 mM phosphate buffer, 1% Triton-X-100 | 8 | 25Β°C | 1 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Methanol | 10% | 100 | 198 | % | 50 mM phosphate buffer, 1% Triton-X-100 | 8 | 25Β°C | 1 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | 1-Propanol | 30% | 100 | 268 | % | 50 mM phosphate buffer, 1% Triton-X-100 | 8 | 25Β°C | 1 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | 1-Propanol | 10% | 100 | 169 | % | 50 mM phosphate buffer, 1% Triton-X-100 | 8 | 25Β°C | 1 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Ethanol | 50% | 100 | 0 | % | 50 mM phosphate buffer, 1% Triton-X-100 | 8 | 25Β°C | 1 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Ethanol | 30% | 100 | 0 | % | 50 mM phosphate buffer, 1% Triton-X-100 | 8 | 25Β°C | 1 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Ethanol | 10% | 100 | 95 | % | 50 mM phosphate buffer, 1% Triton-X-100 | 8 | 25Β°C | 1 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Methanol | 70% | 100 | 63 | % | 50 mM phosphate buffer, 1% Triton-X-100 | 8 | 25Β°C | 1 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Methanol | 50% | 100 | 462 | % | 50 mM phosphate buffer, 1% Triton-X-100 | 8 | 25Β°C | 1 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Methanol | 30% | 100 | 377 | % | 50 mM phosphate buffer, 1% Triton-X-100 | 8 | 25Β°C | 1 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.